>AFO251 (AFO251Q) /CSM/AF/AFO2-C/AFO251Q.Seq.d/
XXXXXXXXXXTTANATTNANCTGGTNGTATNNNNNTNATNNCATNATNNNNNTNTTANNT
GNNNGTGGTTANTCATTCNCANCNNNTGNTGAAANAGNNATCGTTAGAGATATTAAAGAG
AAATTAGCATACGTTGCATTGGATTTTGAAGCTGAAATGCAAACTGCAGCCTCATCATCT
GCATTAGAGAAATCATATGAATTACCAGATGGTCAAGTTATTACAATTGGTAATGAACGT
TTCCGTTGTCCAGAAGCACTTTTCCAACCATCATTCTTGGGTATGGAGTCTGCTGGTATT
CATGAAACAACCTATAATTCAATCATGAAATGTGATGTTGATATTCGTAAAGATTTATAT
GGTAATGTTGTATTATCAGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAAT
AAAGAATTAACTGCACTTGCACCATCAACAATGAAAATTAAAATTATTGCNCCNCCAGAA
NGNAAAAA

---XXXWXYXXXXXXXXLXXXGXSFXXXXEXXIVRDIKEKLAYVALDFEAEMQTAASSSA
LEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYG
NVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPEXK

Frame A:
---xixlvvxxxxhxxxxxxxwlxixxxx*xxxr*ry*reisircigf*s*nancsliic
ireii*itrwssyynw**tfplsrstfptiilgygvcwys*nnl*fnhem*c*ys*rfiw
*cciirwynyvpryc*sye*rinctctinnen*nycxxrxxk


Frame B:
---XXXWXYXXXXXXXXLXXXGXSFXXXXEXXIVRDIKEKLAYVALDFEAEMQTAASSSA
LEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIHETTYNSIMKCDVDIRKDLYG
NVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPEXK


Frame C:
---xxxgxxxxxxxxxxxxxvvxhsxxxxkxxsleilkrn*htlhwilklkcklqphhlh
*rnhmnyqmvkllqlvmnvsvvqkhfsnhhswvwsllvfmkqpiiqs*nvmlifvkiymv
mlyyqvvqlcsqvlliv*ikn*lhlhhqq*klkllxxqxxk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFK545 (SFK545Q) /CSM/SF/SFK5-B/SFK545Q.Seq.d/                        757   0.0
SFJ308 (SFJ308Q) /CSM/SF/SFJ3-A/SFJ308Q.Seq.d/                        757   0.0
SFD477 (SFD477Q) /CSM/SF/SFD4-D/SFD477Q.Seq.d/                        757   0.0
CHE439 (CHE439Q) /CSM/CH/CHE4-B/CHE439Q.Seq.d/                        757   0.0
CHE339 (CHE339Q) /CSM/CH/CHE3-B/CHE339Q.Seq.d/                        757   0.0
CHD733 (CHD733Q) /CSM/CH/CHD7-B/CHD733Q.Seq.d/                        757   0.0
CFF539 (CFF539Q) /CSM/CF/CFF5-B/CFF539Q.Seq.d/                        757   0.0
AFO259 (AFO259Q) /CSM/AF/AFO2-C/AFO259Q.Seq.d/                        757   0.0
AFO251 (AFO251Q) /CSM/AF/AFO2-C/AFO251Q.Seq.d/                        757   0.0
AFN621 (AFN621Q) /CSM/AF/AFN6-A/AFN621Q.Seq.d/                        757   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X03283|X03283.1 Dictyostelium discoideum gene for actin A3-S1.   618  e-173  1
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   587  e-164  1
X03284|X03284.1 Dictyostelium discoideum gene for actin A3-S2.   521  e-144  1
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   517  e-143  7
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   517  e-143  1
X03282|X03282.1 dictyostelium discoideum gene for actin A12.     509  e-141  1
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   470  e-129  1
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.        426  e-115  1
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.   426  e-115  1
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   396  e-107  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   259   2e-68
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   259   2e-68
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        259   2e-68
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   255   3e-67
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   255   3e-67
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   255   3e-67
EF455784_1(EF455784|pid:none) Hyperamoeba sp. ATCC PRA-39 actin ...   255   4e-67
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          254   5e-67
AF508263_1(AF508263|pid:none) Pyrocystis lunula actin mRNA, comp...   252   3e-66
AY221950_1(AY221950|pid:none) Pseudo-nitzschia multiseries actin...   252   3e-66

 psg: 0.84  gvh: 0.59  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 28.0 %: nuclear
	 12.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: Golgi

>> prediction for AFO251 is cyt

>AFO251Z.Seq
----------TTANATTNANCTGGTNGTATNNNNNTNATNNCATNATNNNNNTNTTANNT
GNNNGTGGTTANTCATTCNCANCNNNTGNTGAAANAGNNATCGTTAGAGATATTAAAGAG
AAATTAGCATACGTTGCATTGGATTTTGAAGCTGAAATGCAAACTGCAGCCTCATCATCT
GCATTAGAGAAATCATATGAATTACCAGATGGTCAAGTTATTACAATTGGTAATGAACGT
TTCCGTTGTCCAGAAGCACTTTTCCAACCATCATTCTTGGGTATGGAGTCTGCTGGTATT
CATGAAACAACCTATAATTCAATCATGAAATGTGATGTTGATATTCGTAAAGATTTATAT
GGTAATGTTGTATTATCAGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAAT
AAAGAATTAACTGCACTTGCACCATCAACAATGAAAATTAAAATTATTGCNCCNCCAGAA
NGNAAAAA