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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI533
Library VH
(Link to library)
Clone ID VHI533
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16421-1
Original site URL
Representative seq. ID VHI533P
(Link to Original site)
Representative DNA sequence 
>VHI533 (VHI533Q) /CSM/VH/VHI5-B/VHI533Q.Seq.d/
TCAAGAGGAATACATTAGAGGTTCAATGAATCGTAACCCATATACTTCCAATACCTTTGG
TGGTCCACTCATGAGAGATGTAAAGAATAAGATTTGTAAAGCTTTAGATTTAGGTAGTTT
CTTAGATGATGATAATGGTATGGAGTTATTAGTCGATAATAAGATCATTAAATTGGATTT
ACCAATTAAAAAAGTTTACGAATTAGTATGGAAAAAGAGTCCACAAGCATTAAGAACAGC
AGATATCAATATTCCAATGAATGTAGTTTATCGTCTTCAAGGTTTAGATGGTGAAGCAAC
TGAAGAAATCATTGAAACTCTTAATGATAATAACTCTGAAGAAAAGGATCCAGAAGTTGA
ATATGAAATCACAAGTGTTATGGCTGAATGCGGTGGTTTGGAATCAATGATCTCAATGAT
TGAACGTATCAATGATTTCTCAATCGAGAAGGAATTGGCACAATTAGTAATTAAATTACT
CTACCATTGTTGTAAGATTAAAATTAATCGTCAAAAATTACTTACACTCAATACTGTTGG
TAGATTATTGGAGAAATTAAAACAAGCTTTCCATCAACCAGAACTTTCCGAACXXXXXXX
XXXTTTGGCAAAGGATGAATCTTTCAGTACCGATTNTAAAGGTGGTGGCAAAGAAAGTAA
TATTAGAATTGCACCAATGTTTGTCCAACTTGGTATGTTTTTATTGGATCAAAAGTTGGT
TGGAGGTAACTCTGCCAATCAATTAAGACGTCCACAATTTGAAAAGACTTTAGCACAATT
CACAACTTTACCATTGGAAACTGCAATCACTTCAATGTATCAAATGCTATCCGATAATGT
ACCATACTTTTTAGTGGTTTCAATCTTTTTACATTCACCAAAAGAATTTGAAAATCAAAA
ATTCAATTACCTCACAAAACTATTGGCTTATGCTTTTGTTGATTATCTCACCACTAATAC
AAAGAATGGTTTAAGTTCTCCAGATGAACCATCAAAAGATAAACTTTTCGATATCACTCG
TCCTTGGTTAATATTNTTCTCTTTGTTATCAAAATTCCATTCAATCATTAAACCATCCTC
ATGTACCGAAGAAAATTGGATTCAAGAAANTAAAAATCATTTATCAACAAATTGTACAAA
GATTCAGGTAGATGTTAAAGAATTATTAAATTGTTTCGAAAATGAATTAAAAGAATTCCA
AGATGAAATGGAATTCTTTGATGATGAAGGTCTCTTAAAAAATGTTTTATTAAATAACAA
AGATTCAAATGAATATTTATTAAAATTANATAAATCTGTTAAATCAAATAAAAAATAATT
AAAAAAAATAAAAAAAAATAAATAAATAA
sequence update 2002.10.25
Translated Amino Acid sequence 
QEEYIRGSMNRNPYTSNTFGGPLMRDVKNKICKALDLGSFLDDDNGMELLVDNKIIKLDL
PIKKVYELVWKKSPQALRTADINIPMNVVYRLQGLDGEATEEIIETLNDNNSEEKDPEVE
YEITSVMAECGGLESMISMIERINDFSIEKELAQLVIKLLYHCCKIKINRQKLLTLNTVG
RLLEKLKQAFHQPELSE---

---LAKDESFSTDXKGGGKESNIRIAPMFVQLGMFLLDQKLVGGNSANQLRRPQFEKTLA
QFTTLPLETAITSMYQMLSDNVPYFLVVSIFLHSPKEFENQKFNYLTKLLAYAFVDYLTT
NTKNGLSSPDEPSKDKLFDITRPWLIXFSLLSKFHSIIKPSSCTEENWIQEXKNHLSTNC
TKIQVDVKELLNCFENELKEFQDEMEFFDDEGLLKNVLLNNKDSNEYLLKLXKSVKSNKK
*lkkikknk*i
Translated Amino Acid sequence (All Frames) 
Frame A:
srgih*rfnes*piyfqylwwsthercke*dl*sfrfr*flr***wygvisr**dh*igf
tn*kslrismekestsiknsryqysnecslsssrfrw*sn*rnh*ns****l*rkgsrs*
i*nhkcyg*mrwfgindlnd*tyq*flnregigtisn*itlpll*d*n*sskitytqycw
*iigeiktsfpstrtfr---

---fgkg*ifqyrx*rwwqrk*y*nctnvcptwyvfigskvgwr*lcqsiktsti*kdfs
tihnftigncnhfnvsnair*ctilfsgfnlftftkri*kskiqlphktiglcfc*lshh
*ykewfkfsr*tikr*tfryhsslvnixlfvikipfnh*tilmyrrkldsrx*ksfinkl
ykdsgrc*riiklfrk*ikripr*ngil***rslkkcfik*qrfk*ifikix*ic*ik*k
iikknkkk*in

Frame B:
QEEYIRGSMNRNPYTSNTFGGPLMRDVKNKICKALDLGSFLDDDNGMELLVDNKIIKLDL
PIKKVYELVWKKSPQALRTADINIPMNVVYRLQGLDGEATEEIIETLNDNNSEEKDPEVE
YEITSVMAECGGLESMISMIERINDFSIEKELAQLVIKLLYHCCKIKINRQKLLTLNTVG
RLLEKLKQAFHQPELSE---

---LAKDESFSTDXKGGGKESNIRIAPMFVQLGMFLLDQKLVGGNSANQLRRPQFEKTLA
QFTTLPLETAITSMYQMLSDNVPYFLVVSIFLHSPKEFENQKFNYLTKLLAYAFVDYLTT
NTKNGLSSPDEPSKDKLFDITRPWLIXFSLLSKFHSIIKPSSCTEENWIQEXKNHLSTNC
TKIQVDVKELLNCFENELKEFQDEMEFFDDEGLLKNVLLNNKDSNEYLLKLXKSVKSNKK
*lkkikknk*i

Frame C:
krntlevq*ivthilpiplvvhs*em*rirfvkl*i*vvs*mmimvwsy*siirslnwiy
qlkkftn*ygkrvhkh*eqqisifq*m*fivfkv*mvkqlkkslkllmiitlkkriqkln
mksqvlwlnavvwnq*sq*lnvsmisqsrrnwhn**lnystivvrlklivknylhsillv
dywrn*nklsinqnfpn---

---wqrmnlsvpixkvvakkvilelhqclsnlvcfywikswlevtlpin*dvhnlkrl*h
nsqlyhwklqslqcikcypimyhtf*wfqsfyihqknlkiknsitsqnywlmllliispl
iqrmv*vlqmnhqkinfsislvlg*yxslcyqnsiqslnhphvpkkigfkkxkiiyqqiv
qrfr*mlkny*ivskmn*knskmkwnslmmkvs*kmfy*itkiqmniy*nxinllnqikn
n*kk*kkink*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI533 (VHI533Q) /CSM/VH/VHI5-B/VHI533Q.Seq.d/                       2504   0.0
SHG845 (SHG845Q) /CSM/SH/SHG8-B/SHG845Q.Seq.d/                       1368   0.0
CHG323 (CHG323Q) /CSM/CH/CHG3-A/CHG323Q.Seq.d/                       1368   0.0
AHM536 (AHM536Q) /CSM/AH/AHM5-B/AHM536Q.Seq.d/                       1368   0.0
AHC341 (AHC341Q) /CSM/AH/AHC3-B/AHC341Q.Seq.d/                       1362   0.0
AHK226 (AHK226Q) /CSM/AH/AHK2-B/AHK226Q.Seq.d/                       1350   0.0
CFF581 (CFF581Q) /CSM/CF/CFF5-D/CFF581Q.Seq.d/                       1348   0.0
AHQ309 (AHQ309Q) /CSM/AH/AHQ3-A/AHQ309Q.Seq.d/                       1348   0.0
AHO796 (AHO796Q) /CSM/AH/AHO7-D/AHO796Q.Seq.d/                       1346   0.0
AHK460 (AHK460Q) /CSM/AH/AHK4-C/AHK460Q.Seq.d/                       1336   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ441908) Dictyostelium discoideum cDNA clone:ddv48a09, 3' ...  1267   0.0    1
(BJ407072) Dictyostelium discoideum cDNA clone:dds37j12, 3' ...  1267   0.0    1
(BJ379232) Dictyostelium discoideum cDNA clone:ddc34n05, 3' ...  1267   0.0    1
(BJ356642) Dictyostelium discoideum cDNA clone:dda60p23, 3' ...  1267   0.0    1
(BJ355288) Dictyostelium discoideum cDNA clone:dda56g09, 3' ...  1267   0.0    1
(BJ353734) Dictyostelium discoideum cDNA clone:dda51c08, 3' ...  1265   0.0    1
(BJ357271) Dictyostelium discoideum cDNA clone:dda63b03, 3' ...  1261   0.0    1
(BJ349058) Dictyostelium discoideum cDNA clone:dda35b11, 3' ...  1261   0.0    1
(BJ353835) Dictyostelium discoideum cDNA clone:dda51h16, 3' ...  1251   0.0    1
(BJ372384) Dictyostelium discoideum cDNA clone:ddc12m08, 3' ...  1225   0.0    1
dna update 2008.11.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC151523_5(AC151523|pid:none) Medicago truncatula chromosome 7 c...   197   9e-49
DQ415922_13(DQ415922|pid:none) Cleome spinosa clone BAC Cs1, com...   183   1e-44
AF348492_1(AF348492|pid:none) Homo sapiens p600 mRNA, complete c...   178   5e-43
AB007931_1(AB007931|pid:none) Homo sapiens mRNA for KIAA0462 pro...   178   5e-43
AL137127_2(AL137127|pid:none) Human DNA sequence from clone RP5-...   178   5e-43
(Q2TL32) RecName: Full=E3 ubiquitin-protein ligase UBR4;        ...   176   2e-42
AY850927_1(AY850927|pid:none) Rattus norvegicus ZUBR1 mRNA, comp...   176   2e-42
BC040468_1(BC040468|pid:none) Mus musculus ubiquitin protein lig...   174   5e-42
BC059890_1(BC059890|pid:none) Mus musculus ubiquitin protein lig...   174   5e-42
BC057625_1(BC057625|pid:none) Mus musculus ubiquitin protein lig...   174   5e-42
protein update 2009. 3.11
PSORT 

 psg: 0.84  gvh: 0.33  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for VHI533 is nuc
5' end seq. ID VHI533F
5' end seq. 
>VHI533F.Seq
TCAAGAGGAATACATTAGAGGTTCAATGAATCGTAACCCATATACTTCCAATACCTTTGG
TGGTCCACTCATGAGAGATGTAAAGAATAAGATTTGTAAAGCTTTAGATTTAGGTAGTTT
CTTAGATGATGATAATGGTATGGAGTTATTAGTCGATAATAAGATCATTAAATTGGATTT
ACCAATTAAAAAAGTTTACGAATTAGTATGGAAAAAGAGTCCACAAGCATTAAGAACAGC
AGATATCAATATTCCAATGAATGTAGTTTATCGTCTTCAAGGTTTAGATGGTGAAGCAAC
TGAAGAAATCATTGAAACTCTTAATGATAATAACTCTGAAGAAAAGGATCCAGAAGTTGA
ATATGAAATCACAAGTGTTATGGCTGAATGCGGTGGTTTGGAATCAATGATCTCAATGAT
TGAACGTATCAATGATTTCTCAATCGAGAAGGAATTGGCACAATTAGTAATTAAATTACT
CTACCATTGTTGTAAGATTAAAATTAATCGTCAAAAATTACTTACACTCAATACTGTTGG
TAGATTATTGGAGAAATTAAAACAAGCTTTCCATCAACCAGAACTTTCCGAACNNNNNNN
NNN
Length of 5' end seq. 603
3' end seq. ID VHI533Z
3' end seq. 
>VHI533Z.Seq
NNNNNNNNNNTTTGGCAAAGGATGAATCTTTCAGTACCGATTNTAAAGGTGGTGGCAAAG
AAAGTAATATTAGAATTGCACCAATGTTTGTCCAACTTGGTATGTTTTTATTGGATCAAA
AGTTGGTTGGAGGTAACTCTGCCAATCAATTAAGACGTCCACAATTTGAAAAGACTTTAG
CACAATTCACAACTTTACCATTGGAAACTGCAATCACTTCAATGTATCAAATGCTATCCG
ATAATGTACCATACTTTTTAGTGGTTTCAATCTTTTTACATTCACCAAAAGAATTTGAAA
ATCAAAAATTCAATTACCTCACAAAACTATTGGCTTATGCTTTTGTTGATTATCTCACCA
CTAATACAAAGAATGGTTTAAGTTCTCCAGATGAACCATCAAAAGATAAACTTTTCGATA
TCACTCGTCCTTGGTTAATATTNTTCTCTTTGTTATCAAAATTCCATTCAATCATTAAAC
CATCCTCATGTACCGAAGAAAATTGGATTCAAGAAANTAAAAATCATTTATCAACAAATT
GTACAAAGATTCAGGTAGATGTTAAAGAATTATTAAATTGTTTCGAAAATGAATTAAAAG
AATTCCAAGATGAAATGGAATTCTTTGATGATGAAGGTCTCTTAAAAAATGTTTTATTAA
ATAACAAAGATTCAAATGAATATTTATTAAAATTANATAAATCTGTTAAATCAAATAAAA
AATAATTAAAAAAAATAAAAAAAAATAAATAAATAA
Length of 3' end seq. 756
Connected seq. ID VHI533P
Connected seq. 
>VHI533P.Seq
TCAAGAGGAATACATTAGAGGTTCAATGAATCGTAACCCATATACTTCCAATACCTTTGG
TGGTCCACTCATGAGAGATGTAAAGAATAAGATTTGTAAAGCTTTAGATTTAGGTAGTTT
CTTAGATGATGATAATGGTATGGAGTTATTAGTCGATAATAAGATCATTAAATTGGATTT
ACCAATTAAAAAAGTTTACGAATTAGTATGGAAAAAGAGTCCACAAGCATTAAGAACAGC
AGATATCAATATTCCAATGAATGTAGTTTATCGTCTTCAAGGTTTAGATGGTGAAGCAAC
TGAAGAAATCATTGAAACTCTTAATGATAATAACTCTGAAGAAAAGGATCCAGAAGTTGA
ATATGAAATCACAAGTGTTATGGCTGAATGCGGTGGTTTGGAATCAATGATCTCAATGAT
TGAACGTATCAATGATTTCTCAATCGAGAAGGAATTGGCACAATTAGTAATTAAATTACT
CTACCATTGTTGTAAGATTAAAATTAATCGTCAAAAATTACTTACACTCAATACTGTTGG
TAGATTATTGGAGAAATTAAAACAAGCTTTCCATCAACCAGAACTTTCCGAAC-------
---TTTGGCAAAGGATGAATCTTTCAGTACCGATTNTAAAGGTGGTGGCAAAGAAAGTAA
TATTAGAATTGCACCAATGTTTGTCCAACTTGGTATGTTTTTATTGGATCAAAAGTTGGT
TGGAGGTAACTCTGCCAATCAATTAAGACGTCCACAATTTGAAAAGACTTTAGCACAATT
CACAACTTTACCATTGGAAACTGCAATCACTTCAATGTATCAAATGCTATCCGATAATGT
ACCATACTTTTTAGTGGTTTCAATCTTTTTACATTCACCAAAAGAATTTGAAAATCAAAA
ATTCAATTACCTCACAAAACTATTGGCTTATGCTTTTGTTGATTATCTCACCACTAATAC
AAAGAATGGTTTAAGTTCTCCAGATGAACCATCAAAAGATAAACTTTTCGATATCACTCG
TCCTTGGTTAATATTNTTCTCTTTGTTATCAAAATTCCATTCAATCATTAAACCATCCTC
ATGTACCGAAGAAAATTGGATTCAAGAAANTAAAAATCATTTATCAACAAATTGTACAAA
GATTCAGGTAGATGTTAAAGAATTATTAAATTGTTTCGAAAATGAATTAAAAGAATTCCA
AGATGAAATGGAATTCTTTGATGATGAAGGTCTCTTAAAAAATGTTTTATTAAATAACAA
AGATTCAAATGAATATTTATTAAAATTANATAAATCTGTTAAATCAAATAAAAAATAATT
AAAAAAAATAAAAAAAAATAAATAAATAA
Length of connected seq. 1339
Full length Seq ID -
Full length Seq. -
Length of full length seq. -