NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI525
Library VH
(Link to library)
Clone ID VHI525
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15722-1
Original site URL
Representative seq. ID VHI525P
(Link to Original site)
Representative DNA sequence 
>VHI525 (VHI525Q) /CSM/VH/VHI5-B/VHI525Q.Seq.d/
AAATTTCAATTTAAAAAAAATAAAGGAAAAAACACACCAAAATTAAATAAGTAAGAATAA
AAATGAAATATTTATTAGTATATTTACACATATTACTATTTTGTTTATTAAAGATCAATA
GCCAAAAGTATTGCACTTATAGTGGTGTTGGAGAAGATTATAATATCAAATTTGATGTCT
TACAAACAAATGGTGGTATTGGTTTTCTATACTCATATTTAAGTGTTATTTTTACAACTT
GTAATTATACTATTCCTTGTGGTAGTAATAGTAATAATGTATTGTGTGGATCATATCATT
CAACTGTTCCACGCAAGTATCTTTCATTGGGATTTGTTAATGGCAGTACACATAGTTATA
GCAAAGACGGACTAATGATAACATATATATCACAGGATACAACAAATGATAATGATTGCA
AAAGATATACAACAAATGTGATTTTTCAATGTAGACCAGATGGTGATGTATCCGATTCAT
TAGATGTTGGCATCGACAATATACTTATTGATTCATGTATTGTTGAATTCACACTCTATT
TACGTACAGTGTGTCAAACATGXXXXXXXXXXAAATGGGANCTTGTGATAATAATNCTGG
GAATGGTACATGCCATAATGAACATTTTGGNAATGGTTGTGAATTTACTCGATGTCCACT
CGATTGTTNGACACCAAATGGGTACTTGTGATAATAATACTGGTAATTGTACATGCCATA
ATGAACATTTTGGTAATTCTTGTGAATTTACTCGATGTCCACTCGATTGTTNGACACCAA
ATGGTACTTGTGATAATAATACTGGTAATTGTACATGCCATAATGAACATTTTGGTAATG
GTTGTGAATTCACTCAATGCCCACTCTATTGTTCAACACCAAATGGTACTTGTGATATTA
ATAGTGGTATTTGTACATGTGATAATGAACATATTGGTAATGGTTGCGAAATTAAATTTA
TTGAATGTAAACATAAATGTTCAACCAAACATGGAATATGTGATAATGATAGTGGTAATT
GTAAATGTGATACTCAAACAAAAGGATTAACGTGTGAAGAATCAAGATTATTAATTGAAT
CATTAGACTCAATCAATTCAAAAGGTGGTACAATAAATATAATTGGTTATTTTGGAAATA
CAACATCATTATTAACAATTAAAATTGGTGAATCACAATGTAAAAATATCAAAGTATTCA
ATGAAACAACATTAAAATGTGATATTGGTGAAGGTAAAGGTATTCATAATATTACAATCA
TTGATGATGACCTTTCGTTCACAGCAATCAATAAGTTCCAATATCTCGATGAAAAAACAA
AAATCTCAAAAATATCGAGAGGTGCAATTGCTGGTATTGTTGTTGGTTGTGTTGTTGCTG
TTTCATTAATTGGTTCTATTGCTTTTTATTATCATCACAAAAATAAAAAGGTTAATCTTT
TCTTCTAAAAACTAAAATAAAATTTAACCATTA
sequence update 2002.10.25
Translated Amino Acid sequence 
isi*kk*rkkhtkik*VRIKMKYLLVYLHILLFCLLKINSQKYCTYSGVGEDYNIKFDVL
QTNGGIGFLYSYLSVIFTTCNYTIPCGSNSNNVLCGSYHSTVPRKYLSLGFVNGSTHSYS
KDGLMITYISQDTTNDNDCKRYTTNVIFQCRPDGDVSDSLDVGIDNILIDSCIVEFTLYL
RTVCQT---

---KWXLVIIXLGMVHAIMNILXMVVNLLDVHSIVXHQMGTCDNNTGNCTCHNEHFGNSC
EFTRCPLDCXTPNGTCDNNTGNCTCHNEHFGNGCEFTQCPLYCSTPNGTCDINSGICTCD
NEHIGNGCEIKFIECKHKCSTKHGICDNDSGNCKCDTQTKGLTCEESRLLIESLDSINSK
GGTINIIGYFGNTTSLLTIKIGESQCKNIKVFNETTLKCDIGEGKGIHNITIIDDDLSFT
AINKFQYLDEKTKISKISRGAIAGIVVGCVVAVSLIGSIAFYYHHKNKKVNLFF*klk*n
lti
Translated Amino Acid sequence (All Frames) 
Frame A:
kfqfkknkgkntpklnk*e*k*niy*yiytyyyfvy*rsiaksialivvlekiiisnlms
ykqmvvlvfythi*vlflqlviilflvvvivimycvdhiiqlfhasifhwdllmavhivi
aktd***hiyhriqqmimiakdiqqm*ffnvdqmvmypih*mlastiyllihvllnshsi
yvqcvkh---

---KWXLVIIXLGMVHAIMNILXMVVNLLDVHSIVXHQMGTCDNNTGNCTCHNEHFGNSC
EFTRCPLDCXTPNGTCDNNTGNCTCHNEHFGNGCEFTQCPLYCSTPNGTCDINSGICTCD
NEHIGNGCEIKFIECKHKCSTKHGICDNDSGNCKCDTQTKGLTCEESRLLIESLDSINSK
GGTINIIGYFGNTTSLLTIKIGESQCKNIKVFNETTLKCDIGEGKGIHNITIIDDDLSFT
AINKFQYLDEKTKISKISRGAIAGIVVGCVVAVSLIGSIAFYYHHKNKKVNLFF*klk*n
lti

Frame B:
nfnlkkikekthqn*isknkneifisifthitilfikdq*pkvlhl*wcwrrl*yqi*cl
tnkwwywfsilifkcyfynl*lyyslw*****civwiisfncstqvsfigic*wqyt*l*
qrrtndniyitgynk***lqkiynkcdfsm*trw*cirfircwhrqyty*fmyc*ihtlf
tysvsnm---

---ngxl***xwewymp**tfwxwl*iysmstrlxdtkwvlviiilvivhaimnilvilv
nlldvhsivxhqmvlviiilvivhaimnilvmvvnslnahsivqhqmvlvilivvfvhvi
mnilvmvaklnllnvninvqpnmeyvimivvivnvilkqkd*rvknqdy*lnh*tqsiqk
vvq*i*lvileiqhhy*qlklvnhnvkiskysmkqh*nvilvkvkvfiilqslmmtfrsq
qsissnismkkqksqkyrevqllvlllvvlllfh*lvlllfiiitkikrlifsskn*nki
*pl

Frame C:
isi*kk*rkkhtkik*VRIKMKYLLVYLHILLFCLLKINSQKYCTYSGVGEDYNIKFDVL
QTNGGIGFLYSYLSVIFTTCNYTIPCGSNSNNVLCGSYHSTVPRKYLSLGFVNGSTHSYS
KDGLMITYISQDTTNDNDCKRYTTNVIFQCRPDGDVSDSLDVGIDNILIDSCIVEFTLYL
RTVCQT---

---mgxcdnnxgngtchnehfgngceftrcpldcxtpngyl***yw*lymp**tfw*fl*
iysmstrlxdtkwyl***yw*lymp**tfw*wl*ihsmptllfntkwyl*y**wylym**
*tyw*wlrn*iy*m*t*mfnqtwnm****w*l*m*ysnkrinv*rikiin*iirlnqfkr
wynkynwlfwkyniiinn*nw*itm*kyqsiq*nnikm*yw*r*rys*yynh***pfvhs
nq*vpisr*knknlkniercncwyccwlccccfinwfycfllssqk*kg*sfllktkikf
nh
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI525 (VHI525Q) /CSM/VH/VHI5-B/VHI525Q.Seq.d/                       2383   0.0
VHI141 (VHI141Q) /CSM/VH/VHI1-B/VHI141Q.Seq.d/                       1386   0.0
VHP265 (VHP265Q) /CSM/VH/VHP2-C/VHP265Q.Seq.d/                       1380   0.0
VHH671 (VHH671Q) /CSM/VH/VHH6-C/VHH671Q.Seq.d/                       1380   0.0
VHO766 (VHO766Q) /CSM/VH/VHO7-C/VHO766Q.Seq.d/                       1374   0.0
VHA893 (VHA893Q) /CSM/VH/VHA8-D/VHA893Q.Seq.d/                       1374   0.0
VHQ143 (VHQ143Q) /CSM/VH/VHQ1-B/VHQ143Q.Seq.d/                       1370   0.0
VHK192 (VHK192Q) /CSM/VH/VHK1-D/VHK192Q.Seq.d/                       1368   0.0
FC-BE20 (FC-BE20Q) /CSM/FC/FC-BE/FC-BE20Q.Seq.d/                     1366   0.0
AHH863 (AHH863Q) /CSM/AH/AHH8-C/AHH863Q.Seq.d/                       1358   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ441906) Dictyostelium discoideum cDNA clone:ddv48a07, 3' ...  1435   0.0    2
(BJ443436) Dictyostelium discoideum cDNA clone:ddv52m21, 3' ...  1356   0.0    2
(BJ438489) Dictyostelium discoideum cDNA clone:ddv37h01, 3' ...  1348   0.0    2
(BJ439244) Dictyostelium discoideum cDNA clone:ddv39h24, 3' ...  1346   0.0    2
(BJ445553) Dictyostelium discoideum cDNA clone:ddv59g24, 3' ...  1340   0.0    3
(BJ446250) Dictyostelium discoideum cDNA clone:ddv62j06, 3' ...  1338   0.0    3
(BJ442511) Dictyostelium discoideum cDNA clone:ddv50i04, 3' ...  1334   0.0    2
(BJ437133) Dictyostelium discoideum cDNA clone:ddv33f08, 3' ...  1332   0.0    2
(BJ436905) Dictyostelium discoideum cDNA clone:ddv32h17, 3' ...  1332   0.0    3
(BJ446323) Dictyostelium discoideum cDNA clone:ddv62j08, 3' ...  1320   0.0    3
dna update 2008.11.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC117075_64(AC117075|pid:none) Dictyostelium discoideum chromoso...   125   3e-27
AC116920_20(AC116920|pid:none) Dictyostelium discoideum chromoso...   108   4e-22
AC116100_9(AC116100|pid:none) Dictyostelium discoideum chromosom...   108   4e-22
AC116963_42(AC116963|pid:none) Dictyostelium discoideum chromoso...   107   5e-22
AC116967_8(AC116967|pid:none) Dictyostelium discoideum chromosom...   106   2e-21
AC116920_24(AC116920|pid:none) Dictyostelium discoideum chromoso...   105   3e-21
AC116956_34(AC116956|pid:none) Dictyostelium discoideum chromoso...   101   6e-20
AC116967_7(AC116967|pid:none) Dictyostelium discoideum chromosom...    94   2e-17
AC116920_25(AC116920|pid:none) Dictyostelium discoideum chromoso...    91   1e-16
AC117081_39(AC117081|pid:none) Dictyostelium discoideum chromoso...    90   2e-16
protein update 2009. 7.13
PSORT 

 psg: 0.93  gvh: 0.70  alm: 0.19  top: 0.40  tms: 0.13  mit: 0.32  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 40.0 %: endoplasmic reticulum
	 20.0 %: mitochondrial
	 12.0 %: plasma membrane
	  8.0 %: extracellular, including cell wall
	  4.0 %: Golgi
	  4.0 %: nuclear
	  4.0 %: cytoplasmic
	  4.0 %: vacuolar
	  4.0 %: vesicles of secretory system

>> prediction for VHI525 is end
5' end seq. ID VHI525F
5' end seq. 
>VHI525F.Seq
AAATTTCAATTTAAAAAAAATAAAGGAAAAAACACACCAAAATTAAATAAGTAAGAATAA
AAATGAAATATTTATTAGTATATTTACACATATTACTATTTTGTTTATTAAAGATCAATA
GCCAAAAGTATTGCACTTATAGTGGTGTTGGAGAAGATTATAATATCAAATTTGATGTCT
TACAAACAAATGGTGGTATTGGTTTTCTATACTCATATTTAAGTGTTATTTTTACAACTT
GTAATTATACTATTCCTTGTGGTAGTAATAGTAATAATGTATTGTGTGGATCATATCATT
CAACTGTTCCACGCAAGTATCTTTCATTGGGATTTGTTAATGGCAGTACACATAGTTATA
GCAAAGACGGACTAATGATAACATATATATCACAGGATACAACAAATGATAATGATTGCA
AAAGATATACAACAAATGTGATTTTTCAATGTAGACCAGATGGTGATGTATCCGATTCAT
TAGATGTTGGCATCGACAATATACTTATTGATTCATGTATTGTTGAATTCACACTCTATT
TACGTACAGTGTGTCAAACATGNNNNNNNNNN
Length of 5' end seq. 572
3' end seq. ID VHI525Z
3' end seq. 
>VHI525Z.Seq
NNNNNNNNNNAAATGGGANCTTGTGATAATAATNCTGGGAATGGTACATGCCATAATGAA
CATTTTGGNAATGGTTGTGAATTTACTCGATGTCCACTCGATTGTTNGACACCAAATGGG
TACTTGTGATAATAATACTGGTAATTGTACATGCCATAATGAACATTTTGGTAATTCTTG
TGAATTTACTCGATGTCCACTCGATTGTTNGACACCAAATGGTACTTGTGATAATAATAC
TGGTAATTGTACATGCCATAATGAACATTTTGGTAATGGTTGTGAATTCACTCAATGCCC
ACTCTATTGTTCAACACCAAATGGTACTTGTGATATTAATAGTGGTATTTGTACATGTGA
TAATGAACATATTGGTAATGGTTGCGAAATTAAATTTATTGAATGTAAACATAAATGTTC
AACCAAACATGGAATATGTGATAATGATAGTGGTAATTGTAAATGTGATACTCAAACAAA
AGGATTAACGTGTGAAGAATCAAGATTATTAATTGAATCATTAGACTCAATCAATTCAAA
AGGTGGTACAATAAATATAATTGGTTATTTTGGAAATACAACATCATTATTAACAATTAA
AATTGGTGAATCACAATGTAAAAATATCAAAGTATTCAATGAAACAACATTAAAATGTGA
TATTGGTGAAGGTAAAGGTATTCATAATATTACAATCATTGATGATGACCTTTCGTTCAC
AGCAATCAATAAGTTCCAATATCTCGATGAAAAAACAAAAATCTCAAAAATATCGAGAGG
TGCAATTGCTGGTATTGTTGTTGGTTGTGTTGTTGCTGTTTCATTAATTGGTTCTATTGC
TTTTTATTATCATCACAAAAATAAAAAGGTTAATCTTTTCTTCTAAAAACTAAAATAAAA
TTTAACCATTA
Length of 3' end seq. 911
Connected seq. ID VHI525P
Connected seq. 
>VHI525P.Seq
AAATTTCAATTTAAAAAAAATAAAGGAAAAAACACACCAAAATTAAATAAGTAAGAATAA
AAATGAAATATTTATTAGTATATTTACACATATTACTATTTTGTTTATTAAAGATCAATA
GCCAAAAGTATTGCACTTATAGTGGTGTTGGAGAAGATTATAATATCAAATTTGATGTCT
TACAAACAAATGGTGGTATTGGTTTTCTATACTCATATTTAAGTGTTATTTTTACAACTT
GTAATTATACTATTCCTTGTGGTAGTAATAGTAATAATGTATTGTGTGGATCATATCATT
CAACTGTTCCACGCAAGTATCTTTCATTGGGATTTGTTAATGGCAGTACACATAGTTATA
GCAAAGACGGACTAATGATAACATATATATCACAGGATACAACAAATGATAATGATTGCA
AAAGATATACAACAAATGTGATTTTTCAATGTAGACCAGATGGTGATGTATCCGATTCAT
TAGATGTTGGCATCGACAATATACTTATTGATTCATGTATTGTTGAATTCACACTCTATT
TACGTACAGTGTGTCAAACATG----------AAATGGGANCTTGTGATAATAATNCTGG
GAATGGTACATGCCATAATGAACATTTTGGNAATGGTTGTGAATTTACTCGATGTCCACT
CGATTGTTNGACACCAAATGGGTACTTGTGATAATAATACTGGTAATTGTACATGCCATA
ATGAACATTTTGGTAATTCTTGTGAATTTACTCGATGTCCACTCGATTGTTNGACACCAA
ATGGTACTTGTGATAATAATACTGGTAATTGTACATGCCATAATGAACATTTTGGTAATG
GTTGTGAATTCACTCAATGCCCACTCTATTGTTCAACACCAAATGGTACTTGTGATATTA
ATAGTGGTATTTGTACATGTGATAATGAACATATTGGTAATGGTTGCGAAATTAAATTTA
TTGAATGTAAACATAAATGTTCAACCAAACATGGAATATGTGATAATGATAGTGGTAATT
GTAAATGTGATACTCAAACAAAAGGATTAACGTGTGAAGAATCAAGATTATTAATTGAAT
CATTAGACTCAATCAATTCAAAAGGTGGTACAATAAATATAATTGGTTATTTTGGAAATA
CAACATCATTATTAACAATTAAAATTGGTGAATCACAATGTAAAAATATCAAAGTATTCA
ATGAAACAACATTAAAATGTGATATTGGTGAAGGTAAAGGTATTCATAATATTACAATCA
TTGATGATGACCTTTCGTTCACAGCAATCAATAAGTTCCAATATCTCGATGAAAAAACAA
AAATCTCAAAAATATCGAGAGGTGCAATTGCTGGTATTGTTGTTGGTTGTGTTGTTGCTG
TTTCATTAATTGGTTCTATTGCTTTTTATTATCATCACAAAAATAAAAAGGTTAATCTTT
TCTTCTAAAAACTAAAATAAAATTTAACCATTA
Length of connected seq. 1463
Full length Seq ID -
Full length Seq. -
Length of full length seq. -