>AFO250 (AFO250Q) /CSM/AF/AFO2-C/AFO250Q.Seq.d/
XXXXXXXXXXTAGCAGATCATCCAACCATCGAACGTATTATTACTCCACGTCTTGCATTG
ACCACCGCCGAATATCTTGCATATCAATGTGAAAAACACGTTTTAGTTCTTTTAACCGAT
ATGTCTTCATATGCTGATGCTCTTCGTGAGGTATCTGCTGCTCGTGAAGAAGTACCAGGT
CGTCGTGGTTATCCAGGTTATATGTATACAGATCTTTCAACCATTTACGAACGTGCTGGT
CTATTCAAGGTCGTAATGGTTCTATCACTCAAATTCCAATTTTGACTATGCCAAATGATG
ATATTACCCATCCAATTCCAGATTTAACTGGTTACATTACTGAAGGTCAAATTTTCATTG
ATCGTCAAATCAATAACAGACGAATCTACCCACCAATCAATGTATTACCATCACTTTCTC
GTCTTATGAAATCCGCCATTGGTGACGACATGACCCGTGGCGATCACTCTGAAGTCTCAA
ATCAAATGTATGCCAACTATGCCATCGGTAAAGATGTCCAAGCTATGAAAGCTGTCGTTG
GTGAGGAAGCACTTTCTTCTGAAGATAAACTCTACTTAGAATTTTTAGAACGTTTCGAAT
CTAGCTTTGTCGGTCAAAACCATTATGAAAATCGTGATATCTTTAATTCATTGGATCTTG
GTTGGTCTTTATTAAGAACTTTCCCAAGCAATCTCCTCAAACGTATCACTGAAAAAACTA
TTAAACAATACTATTCACGTTCATCAAAGGGTACTGTAGATTCAACTAACTAAATTTTAA
AAANAAAAAAATA

---srssnhrtyyystscidhrriscism*ktrfssfnryvfic*css*giccs*RSTRS
SWLSRLYVYRSFNHLRTCWSIQGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIFID
RQINNRRIYPPINVLPSLSRLMKSAIGDDMTRGDHSEVSNQMYANYAIGKDVQAMKAVVG
EEALSSEDKLYLEFLERFESSFVGQNHYENRDIFNSLDLGWSLLRTFPSNLLKRITEKTI
KQYYSRSSKGTVDSTN*ilkxkk

Frame A:
---srssnhrtyyystscidhrriscism*ktrfssfnryvfic*css*giccs*RSTRS
SWLSRLYVYRSFNHLRTCWSIQGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIFID
RQINNRRIYPPINVLPSLSRLMKSAIGDDMTRGDHSEVSNQMYANYAIGKDVQAMKAVVG
EEALSSEDKLYLEFLERFESSFVGQNHYENRDIFNSLDLGWSLLRTFPSNLLKRITEKTI
KQYYSRSSKGTVDSTN*ilkxkk


Frame B:
---adhptieriitprlalttaeylayqcekhvlvlltdmssyadalrevsaareevpgr
rgypgymytdlstiyeraglfkvvmvlslkfqf*lcqmmilpiqfqi*lvtllkvkfsli
vksitdesthqsmyyhhflvl*npplvtt*pvaitlksqikcmptmpsvkmskl*klslv
rkhfllkinst*nf*nvsnlalsvktimkivislihwilvgly*elsqaissnvslkkll
nntihvhqrvl*iqltkf*kxkn


Frame C:
---qiiqpsnvlllhvlh*pppnilhinvkntf*ff*piclhmlmlfvrylllvkkyqvv
vviqviciqifqpftnvlvysrs*wfyhsnsnfdyak**yypsnsrfnwlhy*rsnfh*s
snq*qtnlptnqcititfssyeirhw*rhdpwrsl*slksnvcqlchr*rcpsyescrw*
gstff*r*tllrifrtfri*lcrskpl*ks*yl*figswlvfiknfpkqspqtyh*kny*
tilftfikgycrfn*lnfkxkki

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO250 (AFO250Q) /CSM/AF/AFO2-C/AFO250Q.Seq.d/                       1522   0.0
AFM777 (AFM777Q) /CSM/AF/AFM7-D/AFM777Q.Seq.d/                       1501   0.0
AFM756 (AFM756Q) /CSM/AF/AFM7-C/AFM756Q.Seq.d/                       1477   0.0
AFO705 (AFO705Q) /CSM/AF/AFO7-A/AFO705Q.Seq.d/                       1471   0.0
CFC406 (CFC406Q) /CSM/CF/CFC4-A/CFC406Q.Seq.d/                       1469   0.0
VFA667 (VFA667Q) /CSM/VF/VFA6-C/VFA667Q.Seq.d/                       1455   0.0
AFK222 (AFK222Q) /CSM/AF/AFK2-A/AFK222Q.Seq.d/                       1443   0.0
SFC779 (SFC779Q) /CSM/SF/SFC7-D/SFC779Q.Seq.d/                       1435   0.0
AFK619 (AFK619Q) /CSM/AF/AFK6-A/AFK619Q.Seq.d/                       1433   0.0
AFC794 (AFC794Q) /CSM/AF/AFC7-D/AFC794Q.Seq.d/                       1423   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116963|AC116963.1 Dictyostelium discoideum chromosome 2 map 4790445-4846595 strain AX4, *** SEQUENCING IN PROGRESS ***.  1057  0.0  2
AC117076|AC117076.2 Dictyostelium discoideum chromosome 2 map 5862124-6045772 strain AX4, complete sequence.  1057  0.0  2
U63317|U63317.1 Dictyostelium discoideum vacuolar H+ ATPase B subunit (dvacB) mRNA, partial cds.   940  0.0  2
BI516063|BI516063.1 BB160021A20C05 Bee Brain Normalized Library, BB16 Apis mellifera cDNA clone BB160021A20C05 5', mRNA sequence.   151  4e-72  3
BI703972|BI703972.1 kt46e07.y3 Strongyloides ratti L1 pAMP1 v3 Chiapelli McCarter Strongyloides ratti cDNA 5' similar to SW:VATB_CAEEL Q19626 PROBABLE VACUOLAR ATP SYNTHASE SUBUNIT B ;, mRNA sequence.   176  3e-71  4
BI703973|BI703973.1 kt46f03.y3 Strongyloides ratti L1 pAMP1 v3 Chiapelli McCarter Strongyloides ratti cDNA 5' similar to SW:VATB_CAEEL Q19626 PROBABLE VACUOLAR ATP SYNTHASE SUBUNIT B ;, mRNA sequence.   176  9e-70  3
AZ535060|AZ535060.1 ENTBW36TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.   115  4e-55  4
AJ567912|AJ567912.1 Mucor circinelloides partial mRNA for vacuolar ATP synthase subunit  B (vasB gene).   119  6e-53  3
AY231438|AY231438.1 Suaeda maritima subsp. salsa vacuolar H(+)-ATPase subunit B (VHA-B) mRNA, complete cds.    86  9e-52  5
AZ540793|AZ540793.1 ENTFK06TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.   115  5e-51  4

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q76NU1) RecName: Full=V-type proton ATPase subunit B;          ...   352   e-135
U63317_1(U63317|pid:none) Dictyostelium discoideum vacuolar H+ A...   332   e-128
(Q38681) RecName: Full=V-type proton ATPase subunit B 1;        ...   273   e-108
AP002901_17(AP002901|pid:none) Oryza sativa Japonica Group genom...   270   e-107
A31886(A31886) H+-exporting ATPase (EC 3.6.3.6) 57K chain - Arab...   268   e-107
EF153483_1(EF153483|pid:none) Medicago truncatula vacuolar H+-AT...   268   e-107
AK176408_1(AK176408|pid:none) Arabidopsis thaliana mRNA for vacu...   268   e-107
EF114316_1(EF114316|pid:none) Kalidium foliatum vacuolar ATPase ...   268   e-107
(P11574) RecName: Full=V-type proton ATPase subunit B;          ...   268   e-107
AL035540_7(AL035540|pid:none) Arabidopsis thaliana DNA chromosom...   268   e-107

 psg: 0.66  gvh: 0.43  alm: 0.51  top: 0.53  tms: 0.00  mit: 0.64  mip: 0.06
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 88.0 %: mitochondrial
	  8.0 %: nuclear
	  4.0 %: cytoplasmic

>> prediction for AFO250 is mit

>AFO250Z.Seq
----------TAGCAGATCATCCAACCATCGAACGTATTATTACTCCACGTCTTGCATTG
ACCACCGCCGAATATCTTGCATATCAATGTGAAAAACACGTTTTAGTTCTTTTAACCGAT
ATGTCTTCATATGCTGATGCTCTTCGTGAGGTATCTGCTGCTCGTGAAGAAGTACCAGGT
CGTCGTGGTTATCCAGGTTATATGTATACAGATCTTTCAACCATTTACGAACGTGCTGGT
CTATTCAAGGTCGTAATGGTTCTATCACTCAAATTCCAATTTTGACTATGCCAAATGATG
ATATTACCCATCCAATTCCAGATTTAACTGGTTACATTACTGAAGGTCAAATTTTCATTG
ATCGTCAAATCAATAACAGACGAATCTACCCACCAATCAATGTATTACCATCACTTTCTC
GTCTTATGAAATCCGCCATTGGTGACGACATGACCCGTGGCGATCACTCTGAAGTCTCAA
ATCAAATGTATGCCAACTATGCCATCGGTAAAGATGTCCAAGCTATGAAAGCTGTCGTTG
GTGAGGAAGCACTTTCTTCTGAAGATAAACTCTACTTAGAATTTTTAGAACGTTTCGAAT
CTAGCTTTGTCGGTCAAAACCATTATGAAAATCGTGATATCTTTAATTCATTGGATCTTG
GTTGGTCTTTATTAAGAACTTTCCCAAGCAATCTCCTCAAACGTATCACTGAAAAAACTA
TTAAACAATACTATTCACGTTCATCAAAGGGTACTGTAGATTCAACTAACTAAATTTTAA
AAANAAAAAAATA