>AFB666 (AFB666Q) /CSM/AF/AFB6-C/AFB666Q.Seq.d/
XXXXXXXXXXTGTCCAAATGGTTACATTTGTACAATAGTAAATCAATTAGCAGTATGTGT
TTCTCCATCATCCTCTTCGTCAAGTTCTTCATCAACCACTGGCTCACATACTACCACTGG
TGGTTCAACCACTGGCTCACATACTACTACTGGTGGTTCAACCACTGGCTCACATACTAC
TACTGGTGGTTCAACCACTGGCTCACATACTACTACTGGTGGTTCAACCACTGGCTCACA
TACTACTACTGGTGGTTCAACTACTGGTCCAACATGTGGTAATGTTAACTGCCCAAGAGG
TTACCATTGTGAAGTTAGAGGTTCCCAAGCTGTTTGTGTTGCCGATGAATTTGACTCATG
TGCCAATGTTGATTGTGGTTCAGGTTACCATTGTAAGAATGGTGAATGTATTCGTGATAA
AGTAGAATGCGATGCATGTGATCAAATTAACTGCCCAAAGGGTC

---PNGYICTIVNQLAVCVSPSSSSSSSSSTTGSHTTTGGSTTGSHTTTGGSTTGSHTTT
GGSTTGSHTTTGGSTTGSHTTTGGSTTGPTCGNVNCPRGYHCEVRGSQAVCVADEFDSCA
NVDCGSGYHCKNGECIRDKVECDACDQINCPKG

Frame A:
---vqmvtfvq**in*qyvflhhplrqvlhqplahilplvvqplahilllvvqplahill
lvvqplahilllvvqplahilllvvqllvqhvvmltaqevtivklevpklfvlpmnlthv
pmlivvqvtivrmvnvfvik*namhvikltaqrv


Frame B:
---skwlhlynsksissmcfsiilfvkffinhwltyyhwwfnhwltyyywwfnhwltyyy
wwfnhwltyyywwfnhwltyyywwfnywsnmw*c*lpkrlpl*s*rfpsclccr*i*lmc
qc*lwfrlpl*ew*mys**srmrcm*sn*lpkg


Frame C:
---PNGYICTIVNQLAVCVSPSSSSSSSSSTTGSHTTTGGSTTGSHTTTGGSTTGSHTTT
GGSTTGSHTTTGGSTTGSHTTTGGSTTGPTCGNVNCPRGYHCEVRGSQAVCVADEFDSCA
NVDCGSGYHCKNGECIRDKVECDACDQINCPKG

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SFK627 (SFK627Q) /CSM/SF/SFK6-B/SFK627Q.Seq.d/                        900   0.0
CFF146 (CFF146Q) /CSM/CF/CFF1-B/CFF146Q.Seq.d/                        900   0.0
CFD603 (CFD603Q) /CSM/CF/CFD6-A/CFD603Q.Seq.d/                        900   0.0
AFB666 (AFB666Q) /CSM/AF/AFB6-C/AFB666Q.Seq.d/                        900   0.0
SFD365 (SFD365Q) /CSM/SF/SFD3-C/SFD365Q.Seq.d/                        878   0.0
CFD115 (CFD115Q) /CSM/CF/CFD1-A/CFD115Q.Seq.d/                        856   0.0
SFJ181 (SFJ181Q) /CSM/SF/SFJ1-D/SFJ181Q.Seq.d/                        833   0.0
CHD791 (CHD791Q) /CSM/CH/CHD7-D/CHD791Q.Seq.d/                        751   0.0
CFE566 (CFE566Q) /CSM/CF/CFE5-C/CFE566Q.Seq.d/                        751   0.0
CFC468 (CFC468Q) /CSM/CF/CFC4-C/CFC468Q.Seq.d/                        751   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

M26238|M26238.1 D.discoideum spore coat protein SP70 gene, complete cds.   894  0.0  2
AC115584|AC115584.2 Dictyostelium discoideum chromosome 2 map complement(3622543-3568102) strain AX4, complete sequence.   884  0.0  4
X16491|X16491.1 Dictyostelium discoideum sp96 gene for spore coat protein SP96.    34  9e-07  5
S72639|S72639.1 PspB=prespore-specific gene {5' region} [Dictyostelium discoideum, Genomic, 1918 nt].    44  0.025  3
AL414226|AL414226.1 T7 end of clone AW0AA029F08 of library AW0AA from strain CLIB 89 of Yarrowia lipolytica.    28  0.15  4
AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    44  0.83  9
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.          44  1.5  1
AF066071|AF066071.1 Dictyostelium discoideum SP85 (pspB) gene, complete cds.    44  1.5  1
BI222143|BI222143.1 602939084F1 NCI_CGAP_Li9 Mus musculus cDNA clone IMAGE:5102253 5', mRNA sequence.    36  3.7  2
AC118092|AC118092.9 Rattus norvegicus clone CH230-286O15, WORKING DRAFT SEQUENCE, 4 unordered pieces.    30  3.8  7

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P15269) RecName: Full=Spore coat protein SP70; AltName: Full=Pr...   154   9e-37
Z81540_13(Z81540|pid:none) Caenorhabditis elegans Cosmid F46B3, ...    58   8e-08
T22274(T22274)hypothetical protein F46B3.9 - Caenorhabditis eleg...    58   8e-08
S07638(S07638;A60942;B60942) spore coat protein SP96 precursor -...    54   1e-06
(P14328) RecName: Full=Spore coat protein SP96;  &AC117075_49(AC...    54   1e-06
S72639_1(S72639|pid:none) PspB=prespore-specific gene {5' region...    50   2e-05
(P54704) RecName: Full=Spore coat protein SP85; AltName: Full=Ce...    50   2e-05
T22273(T22273)hypothetical protein F46B3.14 - Caenorhabditis ele...    50   2e-05
(P15270) RecName: Full=Spore coat protein SP60; Flags: Precursor...    50   2e-05
X52105_1(X52105|pid:none) Dictyostelium discoideum SP60 gene for...    50   2e-05

 psg: 0.75  gvh: 0.62  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.52  mip: 0.16
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 80.0 %: mitochondrial
	 12.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: cytoplasmic

>> prediction for AFB666 is mit

>AFB666Z.Seq
----------TGTCCAAATGGTTACATTTGTACAATAGTAAATCAATTAGCAGTATGTGT
TTCTCCATCATCCTCTTCGTCAAGTTCTTCATCAACCACTGGCTCACATACTACCACTGG
TGGTTCAACCACTGGCTCACATACTACTACTGGTGGTTCAACCACTGGCTCACATACTAC
TACTGGTGGTTCAACCACTGGCTCACATACTACTACTGGTGGTTCAACCACTGGCTCACA
TACTACTACTGGTGGTTCAACTACTGGTCCAACATGTGGTAATGTTAACTGCCCAAGAGG
TTACCATTGTGAAGTTAGAGGTTCCCAAGCTGTTTGTGTTGCCGATGAATTTGACTCATG
TGCCAATGTTGATTGTGGTTCAGGTTACCATTGTAAGAATGGTGAATGTATTCGTGATAA
AGTAGAATGCGATGCATGTGATCAAATTAACTGCCCAAAGGGTC