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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI485
Library VH
(Link to library)
Clone ID VHI485
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16251-1
Original site URL
Representative seq. ID VHI485P
(Link to Original site)
Representative DNA sequence 
>VHI485 (VHI485Q) /CSM/VH/VHI4-D/VHI485Q.Seq.d/
GAACAACAACAACCACCATCAATTTTAATAGTTCAAACTGAAAAGAGAGATATTGAATCA
ATGTCATTGACTTTCTCATCAGATCCAGAATCATTAAATTCTTCATATAGTAATTGTATA
TTCATAGTTTCAAAAGAACAAAAAGAAAATCCAACTTCATATATTCAAGAATATTTTGAT
ATTACTGAAGTATTCTGTGATAACACTACAATTATTGAAGCTGGTGATAGTGAATTATTA
ACAAAGACAATTGAATCAGGTATTGGTAAAAATGATATTATTTTCTTTTTAGTTTCACTT
GAAGAATTAACAATTGAAAATTATAAACAAGTTACAATGCAATATACATTAGTTAATCAA
ATACTTTTAAGAAATAATCTTTCAACTAGATTTGCTTTATTAACTTACGATTCTCAAAAT
GGTGGTAAAAATTATTTAGGATCATCATTAATTGGTACTTTTAGATACTTTTTAGAATTT
CCATCATTGAATACCTTTTCAATTGATGTCGATAAAGATTCAATTGATAATCTTACATTA
TTCTTACGTTTAGTTGATCTATCTACAATTGGTGATAGAGAXXXXXXXXXXGAAAAAGAT
TGGAAATTAGAAAACTTTATTCTCTTTTCATCAATTGGTGCTATCATTGGTGGTTCAAAA
CAATGTGCATATAGTAGTGCTAATTTAGTATTGGATTCATTATCAAACTATAGAAAGAGT
ATTGGTCTTGCTTCAACTTCAATTAATTGGGGTGGTTTGGATGCTGGTGGTGTTGCCGCC
ACTGATAAATCAGTCGCAAGTTTCTTAGAGGGTCAAGGTATTTTATTGGTTTCTTTATCA
AAGATTTTAGGTTGTCTTGATTCAGTTTTCCAACCATCTAATTCCCATTTATCAAACTTT
ATGCTTTCAAGTTTCAATATTGATAATCTTTTATCTTCTGCACCTCAAATGAAGAGAAAA
ATGGGTCACCACCTAACAAATTATAAAACTTCATCTGCTAGCTCTGATGATTCATTGGGT
GATTCTAGTTCTACTCAAGCAAAAGTCATTTCTACAATCTCTGAATTATTATCAATTCAT
CCATCAAAATTAAATTTAGATACAAGATTAAAAGACTATGGTATTGATTCTTTATTAACA
GTACAATTAAAGAATTGGATAGATAAAGAATTTACAAAGAATTTATTCACTCATTTACAA
TTATCATCAAGTAGTATTAATAGTATCATTCAGAGAATTAGTTCAAAATCAACTTCAACA
TCTACTCCCAATCCAACTAACACCACAAAACAAACCGCAACAACAAAAACATAAGTAGTT
CAAACAACTCCAACTACATT
sequence update 2002.10.25
Translated Amino Acid sequence 
EQQQPPSILIVQTEKRDIESMSLTFSSDPESLNSSYSNCIFIVSKEQKENPTSYIQEYFD
ITEVFCDNTTIIEAGDSELLTKTIESGIGKNDIIFFLVSLEELTIENYKQVTMQYTLVNQ
ILLRNNLSTRFALLTYDSQNGGKNYLGSSLIGTFRYFLEFPSLNTFSIDVDKDSIDNLTL
FLRLVDLSTIGDR---

---EKDWKLENFILFSSIGAIIGGSKQCAYSSANLVLDSLSNYRKSIGLASTSINWGGLD
AGGVAATDKSVASFLEGQGILLVSLSKILGCLDSVFQPSNSHLSNFMLSSFNIDNLLSSA
PQMKRKMGHHLTNYKTSSASSDDSLGDSSSTQAKVISTISELLSIHPSKLNLDTRLKDYG
IDSLLTVQLKNWIDKEFTKNLFTHLQLSSSSINSIIQRISSKSTSTSTPNPTNTTKQTAT
TKT*vvqttptt
Translated Amino Acid sequence (All Frames) 
Frame A:
EQQQPPSILIVQTEKRDIESMSLTFSSDPESLNSSYSNCIFIVSKEQKENPTSYIQEYFD
ITEVFCDNTTIIEAGDSELLTKTIESGIGKNDIIFFLVSLEELTIENYKQVTMQYTLVNQ
ILLRNNLSTRFALLTYDSQNGGKNYLGSSLIGTFRYFLEFPSLNTFSIDVDKDSIDNLTL
FLRLVDLSTIGDR---

---EKDWKLENFILFSSIGAIIGGSKQCAYSSANLVLDSLSNYRKSIGLASTSINWGGLD
AGGVAATDKSVASFLEGQGILLVSLSKILGCLDSVFQPSNSHLSNFMLSSFNIDNLLSSA
PQMKRKMGHHLTNYKTSSASSDDSLGDSSSTQAKVISTISELLSIHPSKLNLDTRLKDYG
IDSLLTVQLKNWIDKEFTKNLFTHLQLSSSSINSIIQRISSKSTSTSTPNPTNTTKQTAT
TKT*vvqttptt

Frame B:
nnnnhhqf**fklkreilnqch*lshqiqnh*ilhivivys*fqknkkkiqlhifknili
llkysvitlqllklvivny*qrqlnqvlvkmilfsf*fhlkn*qlkiinklqcnih*lik
yf*eiifqldlly*ltilkmvvkii*dhh*lvlldtf*nfhh*ipfqlmsikiqliilhy
syv*liylqlvie---

---kkign*ktlfsfhqlvlslvvqnnvhivvli*ywihyqtiervlvllqlqligvvwm
lvvlpplinqsqvs*rvkvfywflyqrf*vvliqfsnhlipiyqtlcfqvsiliifyllh
lk*rekwvtt*qiiklhllalmihwvilvllkqksflqslnyyqfihqn*i*iqd*ktmv
lily*qyn*rig*iknlqriysliynyhqvvlivsfrelvqnqlqhllpiqltpqnkpqq
qkhk*fkqlqlh

Frame C:
tttttinfnssn*kery*invidflirsriikffi**lyihsfkrtkrksnfiysrif*y
y*sil**hyny*sw***iinkdn*iryw*k*yyflfsft*rinn*kl*tsynaiyis*sn
tfkk*sfn*icfinlrfskww*klfriiinwyf*ilfrisiieylfn*cr*rfn**syii
ltfs*siynw**r---

---krleirklyslfinwcyhwwfktmci**c*fsigfiikl*keywscfnfn*lgwfgc
wwccrh**isrkflrgsryfigffikdfrls*fsfpti*fpfiklyafkfqy**sfifct
sneekngsppnkl*nfic*l**fig*f*fysskshfynl*iiinssikikfrykikrlwy
*ffinstikeldr*riykefihsftiiik*y**yhsen*fkinfniysqsn*hhktnrnn
knisssnnsnyi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI485 (VHI485Q) /CSM/VH/VHI4-D/VHI485Q.Seq.d/                       2597   0.0
VHO568 (VHO568Q) /CSM/VH/VHO5-C/VHO568Q.Seq.d/                       1485   0.0
VHH639 (VHH639Q) /CSM/VH/VHH6-B/VHH639Q.Seq.d/                       1485   0.0
VHD848 (VHD848Q) /CSM/VH/VHD8-B/VHD848Q.Seq.d/                       1485   0.0
VHB382 (VHB382Q) /CSM/VH/VHB3-D/VHB382Q.Seq.d/                       1485   0.0
VHB174 (VHB174Q) /CSM/VH/VHB1-D/VHB174Q.Seq.d/                       1485   0.0
VHB109 (VHB109Q) /CSM/VH/VHB1-A/VHB109Q.Seq.d/                       1485   0.0
VHA188 (VHA188Q) /CSM/VH/VHA1-D/VHA188Q.Seq.d/                       1479   0.0
VHH622 (VHH622Q) /CSM/VH/VHH6-A/VHH622Q.Seq.d/                       1477   0.0
VHD102 (VHD102Q) /CSM/VH/VHD1-A/VHD102Q.Seq.d/                       1477   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC116305) Dictyostelium discoideum chromosome 2 map 1005175...  1302   0.0    1
(BJ445768) Dictyostelium discoideum cDNA clone:ddv60g17, 3' ...  1302   0.0    1
(BJ444858) Dictyostelium discoideum cDNA clone:ddv57l17, 3' ...  1302   0.0    1
(BJ441787) Dictyostelium discoideum cDNA clone:ddv47j22, 3' ...  1302   0.0    1
(BJ441341) Dictyostelium discoideum cDNA clone:ddv46m10, 3' ...  1302   0.0    1
(BJ438786) Dictyostelium discoideum cDNA clone:ddv38p12, 3' ...  1302   0.0    1
(BJ438461) Dictyostelium discoideum cDNA clone:ddv37c01, 3' ...  1302   0.0    1
(BJ437286) Dictyostelium discoideum cDNA clone:ddv33d21, 3' ...  1302   0.0    1
(BJ437279) Dictyostelium discoideum cDNA clone:ddv33c19, 3' ...  1302   0.0    1
(BJ437031) Dictyostelium discoideum cDNA clone:ddv33a03, 3' ...  1302   0.0    1
dna update 2008.11.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q869W9) RecName: Full=Probable polyketide synthase 16;         ...   378   e-103
(Q869X2) RecName: Full=Probable polyketide synthase 17;         ...   366   e-100
AC117075_22(AC117075|pid:none) Dictyostelium discoideum chromoso...   149   3e-34
(Q54KU5) RecName: Full=Probable polyketide synthase 24;         ...   145   4e-33
AC117176_58(AC117176|pid:none) Dictyostelium discoideum chromoso...   144   9e-33
(Q54KU3) RecName: Full=Probable polyketide synthase 25;         ...   143   2e-32
(Q54TW0) RecName: Full=Probable polyketide synthase 18;         ...   142   3e-32
AC116982_20(AC116982|pid:none) Dictyostelium discoideum chromoso...   135   3e-30
AC116982_23(AC116982|pid:none) Dictyostelium discoideum chromoso...   134   7e-30
(Q54ED6) RecName: Full=Probable polyketide synthase 41;         ...   131   5e-29
protein update 2009. 7.13
PSORT 

 psg: 0.75  gvh: 0.42  alm: 0.31  top: 0.50  tms: 0.07  mit: 0.13  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.06  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: endoplasmic reticulum
	 20.0 %: nuclear
	 20.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  8.0 %: Golgi
	  8.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for VHI485 is end
5' end seq. ID VHI485F
5' end seq. 
>VHI485F.Seq
GAACAACAACAACCACCATCAATTTTAATAGTTCAAACTGAAAAGAGAGATATTGAATCA
ATGTCATTGACTTTCTCATCAGATCCAGAATCATTAAATTCTTCATATAGTAATTGTATA
TTCATAGTTTCAAAAGAACAAAAAGAAAATCCAACTTCATATATTCAAGAATATTTTGAT
ATTACTGAAGTATTCTGTGATAACACTACAATTATTGAAGCTGGTGATAGTGAATTATTA
ACAAAGACAATTGAATCAGGTATTGGTAAAAATGATATTATTTTCTTTTTAGTTTCACTT
GAAGAATTAACAATTGAAAATTATAAACAAGTTACAATGCAATATACATTAGTTAATCAA
ATACTTTTAAGAAATAATCTTTCAACTAGATTTGCTTTATTAACTTACGATTCTCAAAAT
GGTGGTAAAAATTATTTAGGATCATCATTAATTGGTACTTTTAGATACTTTTTAGAATTT
CCATCATTGAATACCTTTTCAATTGATGTCGATAAAGATTCAATTGATAATCTTACATTA
TTCTTACGTTTAGTTGATCTATCTACAATTGGTGATAGAGANNNNNNNNNN
Length of 5' end seq. 591
3' end seq. ID VHI485Z
3' end seq. 
>VHI485Z.Seq
NNNNNNNNNNGAAAAAGATTGGAAATTAGAAAACTTTATTCTCTTTTCATCAATTGGTGC
TATCATTGGTGGTTCAAAACAATGTGCATATAGTAGTGCTAATTTAGTATTGGATTCATT
ATCAAACTATAGAAAGAGTATTGGTCTTGCTTCAACTTCAATTAATTGGGGTGGTTTGGA
TGCTGGTGGTGTTGCCGCCACTGATAAATCAGTCGCAAGTTTCTTAGAGGGTCAAGGTAT
TTTATTGGTTTCTTTATCAAAGATTTTAGGTTGTCTTGATTCAGTTTTCCAACCATCTAA
TTCCCATTTATCAAACTTTATGCTTTCAAGTTTCAATATTGATAATCTTTTATCTTCTGC
ACCTCAAATGAAGAGAAAAATGGGTCACCACCTAACAAATTATAAAACTTCATCTGCTAG
CTCTGATGATTCATTGGGTGATTCTAGTTCTACTCAAGCAAAAGTCATTTCTACAATCTC
TGAATTATTATCAATTCATCCATCAAAATTAAATTTAGATACAAGATTAAAAGACTATGG
TATTGATTCTTTATTAACAGTACAATTAAAGAATTGGATAGATAAAGAATTTACAAAGAA
TTTATTCACTCATTTACAATTATCATCAAGTAGTATTAATAGTATCATTCAGAGAATTAG
TTCAAAATCAACTTCAACATCTACTCCCAATCCAACTAACACCACAAAACAAACCGCAAC
AACAAAAACATAAGTAGTTCAAACAACTCCAACTACATT
Length of 3' end seq. 759
Connected seq. ID VHI485P
Connected seq. 
>VHI485P.Seq
GAACAACAACAACCACCATCAATTTTAATAGTTCAAACTGAAAAGAGAGATATTGAATCA
ATGTCATTGACTTTCTCATCAGATCCAGAATCATTAAATTCTTCATATAGTAATTGTATA
TTCATAGTTTCAAAAGAACAAAAAGAAAATCCAACTTCATATATTCAAGAATATTTTGAT
ATTACTGAAGTATTCTGTGATAACACTACAATTATTGAAGCTGGTGATAGTGAATTATTA
ACAAAGACAATTGAATCAGGTATTGGTAAAAATGATATTATTTTCTTTTTAGTTTCACTT
GAAGAATTAACAATTGAAAATTATAAACAAGTTACAATGCAATATACATTAGTTAATCAA
ATACTTTTAAGAAATAATCTTTCAACTAGATTTGCTTTATTAACTTACGATTCTCAAAAT
GGTGGTAAAAATTATTTAGGATCATCATTAATTGGTACTTTTAGATACTTTTTAGAATTT
CCATCATTGAATACCTTTTCAATTGATGTCGATAAAGATTCAATTGATAATCTTACATTA
TTCTTACGTTTAGTTGATCTATCTACAATTGGTGATAGAGA----------GAAAAAGAT
TGGAAATTAGAAAACTTTATTCTCTTTTCATCAATTGGTGCTATCATTGGTGGTTCAAAA
CAATGTGCATATAGTAGTGCTAATTTAGTATTGGATTCATTATCAAACTATAGAAAGAGT
ATTGGTCTTGCTTCAACTTCAATTAATTGGGGTGGTTTGGATGCTGGTGGTGTTGCCGCC
ACTGATAAATCAGTCGCAAGTTTCTTAGAGGGTCAAGGTATTTTATTGGTTTCTTTATCA
AAGATTTTAGGTTGTCTTGATTCAGTTTTCCAACCATCTAATTCCCATTTATCAAACTTT
ATGCTTTCAAGTTTCAATATTGATAATCTTTTATCTTCTGCACCTCAAATGAAGAGAAAA
ATGGGTCACCACCTAACAAATTATAAAACTTCATCTGCTAGCTCTGATGATTCATTGGGT
GATTCTAGTTCTACTCAAGCAAAAGTCATTTCTACAATCTCTGAATTATTATCAATTCAT
CCATCAAAATTAAATTTAGATACAAGATTAAAAGACTATGGTATTGATTCTTTATTAACA
GTACAATTAAAGAATTGGATAGATAAAGAATTTACAAAGAATTTATTCACTCATTTACAA
TTATCATCAAGTAGTATTAATAGTATCATTCAGAGAATTAGTTCAAAATCAACTTCAACA
TCTACTCCCAATCCAACTAACACCACAAAACAAACCGCAACAACAAAAACATAAGTAGTT
CAAACAACTCCAACTACATT
Length of connected seq. 1330
Full length Seq ID -
Full length Seq. -
Length of full length seq. -