>VHI484 (VHI484Q) /CSM/VH/VHI4-D/VHI484Q.Seq.d/
XXXXXXXXXXTCTTGGTGATAGTATTACCACTGATCATATTTCACCAGCCGGTAACATCA
ATAGAAAATCATCAGCCGCTCGTTATTTAGAGTCCAAAGGTGTAAAACCAGAAGATTTTA
ACACCTATGGATCTCGTAGAGGTAATGATGAAATTATGGTTAGAGGTACCTTTGCCAATA
CTCGTATTGTTAATAAATTGGCTCCAGCCGTTGGTCCACAAACAACCTATGTACCAACTG
GTGAGTTAATGTTTATTTCAGATGCTGCAGAAAAATATCAATCAGAAGGTCATCAACTCA
TTGTTTTGGCTGGTAGTGATTATGGCTCTGGTAGTTCTAGAGATTGGGCTGCCAAAGGTC
CATACCTTCAAGGAATTAAGTGTGTCATTGCCATCTCATTTGAACGTATTCATAGATCCA
ATCTCGTTGGTATGGGTATTATCCCATTGCAATTCCAACCAGGTCAAAATGCCTCTACTT
TGGGTCTCACTGGTAAAGAACAATTCAATATCGAGTTACCAACTGATAAATCCCTCATTA
AAACTGGTCAAACCGTAAAGGTTACCACTAATTGTGGTAAATCTTTTGAAACTATCCTTC
GTTTCGATACTCCAATCGAAGTTNACATG

---LGDSITTDHISPAGNINRKSSAARYLESKGVKPEDFNTYGSRRGNDEIMVRGTFANT
RIVNKLAPAVGPQTTYVPTGELMFISDAAEKYQSEGHQLIVLAGSDYGSGSSRDWAAKGP
YLQGIKCVIAISFERIHRSNLVGMGIIPLQFQPGQNASTLGLTGKEQFNIELPTDKSLIK
TGQTVKVTTNCGKSFETILRFDTPIEVXM

Frame A:
---LGDSITTDHISPAGNINRKSSAARYLESKGVKPEDFNTYGSRRGNDEIMVRGTFANT
RIVNKLAPAVGPQTTYVPTGELMFISDAAEKYQSEGHQLIVLAGSDYGSGSSRDWAAKGP
YLQGIKCVIAISFERIHRSNLVGMGIIPLQFQPGQNASTLGLTGKEQFNIELPTDKSLIK
TGQTVKVTTNCGKSFETILRFDTPIEVXM


Frame B:
---lvivlpliifhqpvtsienhqplvi*spkv*nqkiltpmdlvevmmklwlevplpil
vllinwlqplvhkqpmyqlvs*clfqmlqkninqkvinslfwlvvimalvvleiglpkvh
tfkelsvslpshlnvfidpislvwvlshcnsnqvkmpllwvslvknnsissyqlinpslk
lvkp*rlplivvnllklsfvsilqskxt


Frame C:
---w**yyh*syftsr*hq*kiisrslfrvqrcktrrf*hlwis*r***nyg*rylcqys
yc**igssrwstnnlctnw*vnvyfrccrkisirrssthcfgw**lwlw*f*rlgcqrsi
psrn*vchchli*tys*iqsrwygyypiaiptrskclyfgshw*rtiqyrvtn**iph*n
wsnrkgyh*lw*if*nypsfrysnrsxh

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI484 (VHI484Q) /CSM/VH/VHI4-D/VHI484Q.Seq.d/                       1221   0.0
VHQ461 (VHQ461Q) /CSM/VH/VHQ4-C/VHQ461Q.Seq.d/                       1215   0.0
VHQ432 (VHQ432Q) /CSM/VH/VHQ4-B/VHQ432Q.Seq.d/                       1215   0.0
VHQ267 (VHQ267Q) /CSM/VH/VHQ2-C/VHQ267Q.Seq.d/                       1215   0.0
VHO844 (VHO844Q) /CSM/VH/VHO8-B/VHO844Q.Seq.d/                       1215   0.0
VHO678 (VHO678Q) /CSM/VH/VHO6-D/VHO678Q.Seq.d/                       1215   0.0
VHB664 (VHB664Q) /CSM/VH/VHB6-C/VHB664Q.Seq.d/                       1215   0.0
VHB318 (VHB318Q) /CSM/VH/VHB3-A/VHB318Q.Seq.d/                       1215   0.0
VHA714 (VHA714Q) /CSM/VH/VHA7-A/VHA714Q.Seq.d/                       1215   0.0
VHA182 (VHA182Q) /CSM/VH/VHA1-D/VHA182Q.Seq.d/                       1215   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BQ511380|BQ511380.2 EST618795 Generation of a set of potato cDNA clones for microarray5' end, mRNA sequence.    58   6e-17  3
BI927352|BI927352.1 EST547241 tomato flower, 3 - 8 mm buds Lycopersicon esculentum cDNA clone cTOB22E7 5' end, mRNA sequence.    58   1e-15  3
AW622039|AW622039.1 EST312837 tomato root during/after fruit set, Cornell University Lycopersicon esculentum cDNA clone cLEX14G21 5', mRNA sequence.    58   2e-15  3
BG889764|BG889764.1 EST515615 cSTD Solanum tuberosum cDNA clone cSTD15K20 5' sequence, mRNA sequence.    58   2e-15  3
AF194945|AF194945.1 Nicotiana tabacum cytosolic aconitase (ACO) mRNA, complete cds.    66   2e-15  3
AI775579|AI775579.1 EST256679 tomato resistant, Cornell Lycopersicon esculentum cDNA clone cLER15P4, mRNA sequence.    58   4e-14  2
AW455290|AW455290.1 EST311828 tomato root during/after fruit set, Cornell University Lycopersicon esculentum cDNA clone cLEX10C9 5', mRNA sequence.    58   5e-13  2
BM062502|BM062502.1 KS01043F01 KS01 Capsicum annuum cDNA, mRNA sequence.    58   6e-13  2
CK270637|CK270637.1 EST716715 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POACU43 5' end, mRNA sequence.    58   9e-13  2
BU032661|BU032661.1 QHJ21N12.yg.ab1 QH_EFGHJ sunflower RHA280 Helianthus annuus cDNA clone QHJ21N12, mRNA sequence.    54   5e-12  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54X73) RecName: Full=Probable cytoplasmic aconitate hydratase;...   409   e-113
AB236788_1(AB236788|pid:none) Trifolium pratense RNA for putativ...   275   1e-72
BT060665_1(BT060665|pid:none) Zea mays full-length cDNA clone ZM...   273   2e-72
BT060820_1(BT060820|pid:none) Zea mays full-length cDNA clone ZM...   273   3e-72
AP008209_256(AP008209|pid:none) Oryza sativa (japonica cultivar-...   273   3e-72
(Q23500) RecName: Full=Probable cytoplasmic aconitate hydratase;...   271   1e-71
(Q9SIB9) RecName: Full=Aconitate hydratase 2, mitochondrial;    ...   270   3e-71
(P49608) RecName: Full=Aconitate hydratase, cytoplasmic;        ...   268   9e-71
AY050485_1(AY050485|pid:none) Prunus avium putative aconitase mR...   267   2e-70
DQ294230_1(DQ294230|pid:none) Glossina morsitans morsitans TC165...   266   4e-70

 psg: 0.75  gvh: 0.26  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 36.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for VHI484 is nuc

>VHI484Z.Seq
NNNNNNNNNNTCTTGGTGATAGTATTACCACTGATCATATTTCACCAGCCGGTAACATCA
ATAGAAAATCATCAGCCGCTCGTTATTTAGAGTCCAAAGGTGTAAAACCAGAAGATTTTA
ACACCTATGGATCTCGTAGAGGTAATGATGAAATTATGGTTAGAGGTACCTTTGCCAATA
CTCGTATTGTTAATAAATTGGCTCCAGCCGTTGGTCCACAAACAACCTATGTACCAACTG
GTGAGTTAATGTTTATTTCAGATGCTGCAGAAAAATATCAATCAGAAGGTCATCAACTCA
TTGTTTTGGCTGGTAGTGATTATGGCTCTGGTAGTTCTAGAGATTGGGCTGCCAAAGGTC
CATACCTTCAAGGAATTAAGTGTGTCATTGCCATCTCATTTGAACGTATTCATAGATCCA
ATCTCGTTGGTATGGGTATTATCCCATTGCAATTCCAACCAGGTCAAAATGCCTCTACTT
TGGGTCTCACTGGTAAAGAACAATTCAATATCGAGTTACCAACTGATAAATCCCTCATTA
AAACTGGTCAAACCGTAAAGGTTACCACTAATTGTGGTAAATCTTTTGAAACTATCCTTC
GTTTCGATACTCCAATCGAAGTTNACATG