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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI411
Library VH
(Link to library)
Clone ID VHI411
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VHI411P
(Link to Original site)
Representative DNA sequence 
>VHI411 (VHI411Q) /CSM/VH/VHI4-A/VHI411Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGANATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGXXXXXXXXXXATATCTCATCCAAAGATGATCCAAAAGCTCGTGCTAACTATCTC
GCTGATAACCACGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAA
GGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATT
AAAGATTCTTCCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAA
AACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCAC
AGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACT
GCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCT
ATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGT
CGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGT
TTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGAT
CATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCC
ACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAA
CTCTAAACAATTTAAGTCGCACTTGAGTTTTCAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRXMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTE---

---ISSKDDPKARANYLADNHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDS
SVGAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASP
TLLEPIYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTA
DLRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*t
i*valefs
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkisxicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlk---

---ishpkmiqklvltislittngikmmp*tfglsvqkvtvpisssmspkvfni*mklki
lplvlsngppkkvlfvmkt*evsvsifmmspstlmpstevvvkssqllvvysmlln*lpp
qls*nqst**kllpqktllvvftlysivvevlllvknvvsvphyslskliyqy*npsvsp
liyvptlpvklshnvssiiglqlvlstkirkplksl*pperekv*pqksqiltnstknsk
qfkshlsfq

Frame B:
tleckkskvqng*fhnrsnschygps*kyqxyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*h*---

---yliqr*skssc*lsr**prmg*k*chehlvfrsrr*rcqsprqchqrcsife*n*rf
frwcfpmghqrrccl**kherypfqsl*chpph*chpqrwwsnhpncssctlcc*inclp
nslrtnllsrnycprkrywwyllctqss*rycyw*rtsyrfpiilcqssftsirilrfhr
*ftfphcrssfptmclrslgfnwccqqr*esh*srfshqkekrfsprnprs*qiprktln
nlsrt*vfk

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRXMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTE---

---ISSKDDPKARANYLADNHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDS
SVGAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASP
TLLEPIYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTA
DLRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*t
i*valefs
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI411 (VHI411Q) /CSM/VH/VHI4-A/VHI411Q.Seq.d/                       2581   0.0
VHK562 (VHK562Q) /CSM/VH/VHK5-C/VHK562Q.Seq.d/                       2567   0.0
VHP324 (VHP324Q) /CSM/VH/VHP3-A/VHP324Q.Seq.d/                       1457   0.0
VHH853 (VHH853Q) /CSM/VH/VHH8-C/VHH853Q.Seq.d/                       1457   0.0
VHC653 (VHC653Q) /CSM/VH/VHC6-C/VHC653Q.Seq.d/                       1457   0.0
VHC551 (VHC551Q) /CSM/VH/VHC5-C/VHC551Q.Seq.d/                       1457   0.0
VHO742 (VHO742Q) /CSM/VH/VHO7-B/VHO742Q.Seq.d/                       1455   0.0
VHB665 (VHB665Q) /CSM/VH/VHB6-C/VHB665Q.Seq.d/                       1455   0.0
VHP519 (VHP519Q) /CSM/VH/VHP5-A/VHP519Q.Seq.d/                       1453   0.0
VHO865 (VHO865Q) /CSM/VH/VHO8-C/VHO865Q.Seq.d/                       1453   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ441552) Dictyostelium discoideum cDNA clone:ddv47f04, 3' ...  1465   0.0    1
(BJ445968) Dictyostelium discoideum cDNA clone:ddv61p05, 3' ...  1457   0.0    1
(BJ441409) Dictyostelium discoideum cDNA clone:ddv46j14, 3' ...  1457   0.0    1
(BJ438176) Dictyostelium discoideum cDNA clone:ddv36i14, 3' ...  1457   0.0    1
(BJ438155) Dictyostelium discoideum cDNA clone:ddv36e13, 3' ...  1457   0.0    1
(BJ445681) Dictyostelium discoideum cDNA clone:ddv60d11, 3' ...  1455   0.0    1
(BJ443344) Dictyostelium discoideum cDNA clone:ddv52k15, 3' ...  1455   0.0    1
(BJ437508) Dictyostelium discoideum cDNA clone:ddv34a18, 3' ...  1455   0.0    1
(BJ446222) Dictyostelium discoideum cDNA clone:ddv62e05, 3' ...  1453   0.0    1
(BJ445744) Dictyostelium discoideum cDNA clone:ddv60b18, 3' ...  1453   0.0    1
dna update 2008.11.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   304   6e-81
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   302   2e-80
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   299   2e-79
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   298   4e-79
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   297   5e-79
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   297   5e-79
AK032997_1(AK032997|pid:none) Mus musculus 12 days embryo male w...   297   5e-79
(P05197) RecName: Full=Elongation factor 2;          Short=EF-2;...   297   5e-79
AJ278147_1(AJ278147|pid:none) Rattus norvegicus partial Ef-2 gen...   297   5e-79
BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   297   5e-79
protein update 2009. 7.13
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHI411 is cyt
5' end seq. ID VHI411F
5' end seq. 
>VHI411F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGANATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGNNNNNNNNNN
Length of 5' end seq. 616
3' end seq. ID VHI411Z
3' end seq. 
>VHI411Z.Seq
NNNNNNNNNNATATCTCATCCAAAGATGATCCAAAAGCTCGTGCTAACTATCTCGCTGAT
AACCACGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAAC
GGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGAT
TCTTCCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATG
AGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGT
GGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCC
CCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGT
GGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATC
GGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACC
GCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGG
GCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGA
AAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAA
ACAATTTAAGTCGCACTTGAGTTTTCAAA
Length of 3' end seq. 749
Connected seq. ID VHI411P
Connected seq. 
>VHI411P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGANATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAG----------ATATCTCATCCAAAGATGATCCAAAAGCTCGTGCTAACTATCTC
GCTGATAACCACGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAA
GGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATT
AAAGATTCTTCCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAA
AACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCAC
AGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACT
GCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCT
ATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGT
CGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGT
TTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGAT
CATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCC
ACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAA
CTCTAAACAATTTAAGTCGCACTTGAGTTTTCAAA
Length of connected seq. 1345
Full length Seq ID -
Full length Seq. -
Length of full length seq. -