NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI401
Library VH
(Link to library)
Clone ID VHI401
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHI401P
(Link to Original site)
Representative DNA sequence 
>VHI401 (VHI401Q) /CSM/VH/VHI4-A/VHI401Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTGTATCCCCAGXXXXXXXXXXAACGGTGCCAA
TCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGT
TGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTAT
CCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGGTGGTCA
AATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTCT
CTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTA
CTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCC
ATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTT
ACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAAT
TGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAA
AGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTA
AGTCGCACTTNAGTTTTCAAANATAGTAATCAAATCTAAAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTEDKEFGDVTVSP---

---NGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLHTDA
IHRGGGQIIPTARRVLYAAELTASPTLLEPIYLVEITAPENAIGGIYSVLNRRRGIVIGE
ERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVA
LATRKRKGLAPEIPDLDKFHEKL*ti*valxfsxivikskk
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlkirnsvmslypq---

---NGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLHTDA
IHRGGGQIIPTARRVLYAAELTASPTLLEPIYLVEITAPENAIGGIYSVLNRRRGIVIGE
ERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVA
LATRKRKGLAPEIPDLDKFHEKL*ti*valxfsxivikskk

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*h*r*gir*chcip---

---tvpisssmspkvfni*mklkilslvlsngppkkvlfvmkt*evsvsifmmspstlmp
stevvvkssqllvvysmlln*lppqls*nqst**kllpqktllvvftlysivvevlllvk
nvvsvphyslskliyqy*npsvspliyvptlpvklshnvssiiglqlvlstkirkplksl
*pperekv*pqksqiltnstknskqfkshxsfqx**snlkk

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTEDKEFGDVTVSP---

---rcqsprqchqrcsife*n*rffrwcfpmghqrrccl**kherypfqsl*chpph*ch
pqrwwsnhpncssctlcc*inclpnslrtnllsrnycprkrywwyllctqss*rycyw*r
tsyrfpiilcqssftsirilrfhr*ftfphcrssfptmclrslgfnwccqqr*esh*srf
shqkekrfsprnprs*qiprktlnnlsrtxvfkxsnqi*kk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ491 (VHQ491Q) /CSM/VH/VHQ4-D/VHQ491Q.Seq.d/                       1273   0.0
VHQ476 (VHQ476Q) /CSM/VH/VHQ4-D/VHQ476Q.Seq.d/                       1273   0.0
VHQ453 (VHQ453Q) /CSM/VH/VHQ4-C/VHQ453Q.Seq.d/                       1273   0.0
VHQ434 (VHQ434Q) /CSM/VH/VHQ4-B/VHQ434Q.Seq.d/                       1273   0.0
VHQ410 (VHQ410Q) /CSM/VH/VHQ4-A/VHQ410Q.Seq.d/                       1273   0.0
VHQ359 (VHQ359Q) /CSM/VH/VHQ3-C/VHQ359Q.Seq.d/                       1273   0.0
VHQ351 (VHQ351Q) /CSM/VH/VHQ3-C/VHQ351Q.Seq.d/                       1273   0.0
VHQ212 (VHQ212Q) /CSM/VH/VHQ2-A/VHQ212Q.Seq.d/                       1273   0.0
VHQ208 (VHQ208Q) /CSM/VH/VHQ2-A/VHQ208Q.Seq.d/                       1273   0.0
VHQ191 (VHQ191Q) /CSM/VH/VHQ1-D/VHQ191Q.Seq.d/                       1273   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ446821) Dictyostelium discoideum cDNA clone:ddv63o19, 3' ...  1273   0.0    1
(BJ446805) Dictyostelium discoideum cDNA clone:ddv63k21, 3' ...  1273   0.0    1
(BJ446787) Dictyostelium discoideum cDNA clone:ddv63h20, 3' ...  1273   0.0    1
(BJ446779) Dictyostelium discoideum cDNA clone:ddv63f24, 3' ...  1273   0.0    1
(BJ446772) Dictyostelium discoideum cDNA clone:ddv63e23, 3' ...  1273   0.0    1
(BJ446716) Dictyostelium discoideum cDNA clone:ddv63j14, 3' ...  1273   0.0    1
(BJ446712) Dictyostelium discoideum cDNA clone:ddv63i13, 3' ...  1273   0.0    1
(BJ446698) Dictyostelium discoideum cDNA clone:ddv63f15, 3' ...  1273   0.0    1
(BJ446696) Dictyostelium discoideum cDNA clone:ddv63f13, 3' ...  1273   0.0    1
(BJ446612) Dictyostelium discoideum cDNA clone:ddv63d10, 3' ...  1273   0.0    1
dna update 2008.11.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   273   1e-71
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   269   2e-70
AM494972_26(AM494972|pid:none) Leishmania braziliensis chromosom...   269   2e-70
CT005272_19(CT005272|pid:none) Leishmania major strain Friedlin,...   268   2e-70
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   268   3e-70
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   268   4e-70
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   266   9e-70
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   266   2e-69
AY618550_1(AY618550|pid:none) Trypanosoma cruzi elongation facto...   265   2e-69
CU638744_366(CU638744|pid:none) Podospora anserina genomic DNA c...   264   5e-69
protein update 2009. 7.13
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHI401 is cyt
5' end seq. ID VHI401F
5' end seq. 
>VHI401F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTGTATCCCCAGNNNNNNNNNN
Length of 5' end seq. 649
3' end seq. ID VHI401Z
3' end seq. 
>VHI401Z.Seq
NNNNNNNNNNAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAA
TGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTG
TGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGC
CATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGA
ATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGA
AAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGA
AGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATC
CTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGT
CTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGC
TTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCA
CGAAAAACTCTAAACAATTTAAGTCGCACTTNAGTTTTCAAANATAGTAATCAAATCTAA
AAAAAA
Length of 3' end seq. 666
Connected seq. ID VHI401P
Connected seq. 
>VHI401P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTGTATCCCCAG----------AACGGTGCCAA
TCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGT
TGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTAT
CCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGGTGGTCA
AATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTCT
CTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTA
CTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCC
ATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTT
ACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAAT
TGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAA
AGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTA
AGTCGCACTTNAGTTTTCAAANATAGTAATCAAATCTAAAAAAAA
Length of connected seq. 1295
Full length Seq ID -
Full length Seq. -
Length of full length seq. -