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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI396
Library VH
(Link to library)
Clone ID VHI396
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U16363-1
Original site URL
Representative seq. ID VHI396P
(Link to Original site)
Representative DNA sequence 
>VHI396 (VHI396Q) /CSM/VH/VHI3-D/VHI396Q.Seq.d/
AAAAAGAATGAAAAGATCAATAGTATTTATTTATTCATTGGTAATTTTATTATTATCAGT
TGGGTTTATTGATGCATTCAAAATATCAATAGAAAATCATATAAAATTATCAGATGATTC
ATCTTATCAAATTGGTACAGGTATTTATGATATTACCGGACCAGGTGCAGAGACTAATAT
GATGGGCTATGCAATGCCAGGCCAAATTACTGGAGGAATTCATTTTAGACAACGTGCTAG
AGCATTTGTATTTATAGATTCAGAAGGTAATAGAGCAGTATATGTTAGTACTGATTCATG
TATGATTTTCCAAGAAGTTAAAATTCAAGTTATTCAAGATTTACAAGAAATTTTTGGACC
AACTTTATATACACATGATAATGTATTATTAAGCGGTACACATACTCATTCAGGTCCTGC
TGGTTTTTCAGAGTATGCATTATATGGTATTACAGCATTAGGTTTCTATAAAAAGAATTT
CGATACAATTTGTGATGGTATTGTTCAAGCAATTGTTAAAGCTCATAAATCGGTTCAACC
TGCAAGAATGTTAACTCAACAXXXXXXXXXXGGTCAAATTGTTTTAGTCGCTGTTCCAGG
TGAATTCACAACAATGTCTGGTAGAAGATTAAGAAATACCGTTAGAGAAATCATTGGTCA
ATCAATTGAAAATCCAATTGTTTTAATTGCTGGTTTAGCAAATACTTATTCAGGTTATAT
TGCAACATTTGAAGAGTTTCAAGTTCAAAGATATGAAGGTGCATCAACCGTTTTTGGACC
TCATACTTTAGGAGCATATCAACAAGAATTTGCAAATCTTGCACAATCAATTGTAGATGG
TAGTCAAGCTGATCCAGGAACATTCCCAAGAAATATGTCTGGTCATACACCATTCTTTTT
ACCACCAGTCATTGTTGATGTAGCTCCAAAATTCGACGATTTCGGTGATATCTATACTGA
TGTCTCTACAACTACACCATATTCAATCAATCAAACTGTTACCGTCATTTTCTATGGTGC
CAATCTTAGAAATAATTTCATGACTGAATCTTCTTTCCTCACTGTTGATCAATTACAATC
TAATGGTCAATGGACTACAATTTTAAATGATGGTGATTGGGATACAAAATTATATTGGAA
AATGCATGATTTAGGTTTTTCACTCATCACTGTTGATTGGACAATCTCTCCAATCACTCA
ACCAGGTACTTATAGAATAACTCATAGTGGTTATGCTAAGAAAAATCCATTCTCTGATAA
TTTAACTTTTTATCAAGGTATTTCAAGTAATTTTAATGTTCAATAAAAATTAAAATAAAT
AAAAAAAGGATTTTTTTTT
sequence update 2002.10.25
Translated Amino Acid sequence 
KRMKRSIVFIYSLVILLLSVGFIDAFKISIENHIKLSDDSSYQIGTGIYDITGPGAETNM
MGYAMPGQITGGIHFRQRARAFVFIDSEGNRAVYVSTDSCMIFQEVKIQVIQDLQEIFGP
TLYTHDNVLLSGTHTHSGPAGFSEYALYGITALGFYKKNFDTICDGIVQAIVKAHKSVQP
ARMLTQ---

---GQIVLVAVPGEFTTMSGRRLRNTVREIIGQSIENPIVLIAGLANTYSGYIATFEEFQ
VQRYEGASTVFGPHTLGAYQQEFANLAQSIVDGSQADPGTFPRNMSGHTPFFLPPVIVDV
APKFDDFGDIYTDVSTTTPYSINQTVTVIFYGANLRNNFMTESSFLTVDQLQSNGQWTTI
LNDGDWDTKLYWKMHDLGFSLITVDWTISPITQPGTYRITHSGYAKKNPFSDNLTFYQGI
SSNFNVQ*klk*ikkgfff
Translated Amino Acid sequence (All Frames) 
Frame A:
kknekinsiylfignfiiiswvy*ciqninrksykiir*filsnwyryl*yyrtrcrd*y
dglcnarpnywrnsf*ttc*siciyrfrr**ssic*y*fmydfprs*nssysrftrnfwt
nfiyt**ciikrytysfrscwffrvciiwyysirfl*kefrynl*wycssnc*ss*igst
cknvnst---

---GQIVLVAVPGEFTTMSGRRLRNTVREIIGQSIENPIVLIAGLANTYSGYIATFEEFQ
VQRYEGASTVFGPHTLGAYQQEFANLAQSIVDGSQADPGTFPRNMSGHTPFFLPPVIVDV
APKFDDFGDIYTDVSTTTPYSINQTVTVIFYGANLRNNFMTESSFLTVDQLQSNGQWTTI
LNDGDWDTKLYWKMHDLGFSLITVDWTISPITQPGTYRITHSGYAKKNPFSDNLTFYQGI
SSNFNVQ*klk*ikkgfff

Frame B:
KRMKRSIVFIYSLVILLLSVGFIDAFKISIENHIKLSDDSSYQIGTGIYDITGPGAETNM
MGYAMPGQITGGIHFRQRARAFVFIDSEGNRAVYVSTDSCMIFQEVKIQVIQDLQEIFGP
TLYTHDNVLLSGTHTHSGPAGFSEYALYGITALGFYKKNFDTICDGIVQAIVKAHKSVQP
ARMLTQ---

---vklf*slfqvnsqqclved*eiplekslvnqlkiqlf*llv*qiliqvilqhlksfk
fkdmkvhqpfldlil*ehinknlqilhnql*mvvkliqehsqeiclvihhsfyhqsllm*
lqnstisvisilmslqlhhiqsikllpsfsmvpileiis*lnllssllinynlmvnglqf
*mmvigiqnyigkcmi*vfhsslligqslqslnqvlie*livvmlrkihslii*lfikvf
qvilmfnkn*nk*kkdff

Frame C:
ke*kdq*ylfihw*fyyyqlgllmhskyq*kii*nyqmihliklvqvfmilpdqvqrli*
wamqcqaklleefildnvlehlyl*iqkvieqymlvlihv*fskklkfklfkiykkfldq
lyihmimyy*avhiliqvllvfqsmhymvlqh*vsikrisiqfvmvlfkqllklinrfnl
qec*ln---

---sncfsrcsr*ihnnvw*kikkyr*rnhwsin*ksncfncwfskylfrlycni*rvss
ski*rcinrfwtsyfrsistricksctincrw*ss*srnipkkyvwsytilfttshc*cs
skirrfr*yly*clynytifnqsncyrhflwcqs*k*fhd*iffphc*siti*wsmdynf
k*w*lgykiilena*frffthhc*ldnlsnhstryl*nns*wlc*eksil**fnflsryf
k*f*csikikinkkriff
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI396 (VHI396Q) /CSM/VH/VHI3-D/VHI396Q.Seq.d/                       2577   0.0
VHD145 (VHD145Q) /CSM/VH/VHD1-B/VHD145Q.Seq.d/                       1463   0.0
VHA429 (VHA429Q) /CSM/VH/VHA4-B/VHA429Q.Seq.d/                       1455   0.0
VHD412 (VHD412Q) /CSM/VH/VHD4-A/VHD412Q.Seq.d/                       1427   0.0
VHB215 (VHB215Q) /CSM/VH/VHB2-A/VHB215Q.Seq.d/                       1421   0.0
VFM574 (VFM574Q) /CSM/VF/VFM5-D/VFM574Q.Seq.d/                       1403   0.0
AHB843 (AHB843Q) /CSM/AH/AHB8-B/AHB843Q.Seq.d/                       1402   0.0
VHP543 (VHP543Q) /CSM/VH/VHP5-B/VHP543Q.Seq.d/                       1400   0.0
AHM465 (AHM465Q) /CSM/AH/AHM4-C/AHM465Q.Seq.d/                       1400   0.0
SFK788 (SFK788Q) /CSM/SF/SFK7-D/SFK788Q.Seq.d/                       1396   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(U82513) Dictyostelium discoideum random slug cDNA25 protein...  1449   0.0    1
(AB121061) Dictyostelium discoideum dcd2A mRNA for ceramidas...  1449   0.0    1
(BJ441820) Dictyostelium discoideum cDNA clone:ddv47p23, 3' ...  1449   0.0    1
(BJ438581) Dictyostelium discoideum cDNA clone:ddv37i11, 3' ...  1441   0.0    1
(BJ436500) Dictyostelium discoideum cDNA clone:ddv31j08, 3' ...  1423   0.0    1
(BJ437092) Dictyostelium discoideum cDNA clone:ddv33m04, 3' ...  1402   0.0    1
(BJ438492) Dictyostelium discoideum cDNA clone:ddv37h04, 3' ...  1376   0.0    1
(BJ355023) Dictyostelium discoideum cDNA clone:dda55b18, 3' ...  1356   0.0    1
(BJ435316) Dictyostelium discoideum cDNA clone:ddv26c19, 3' ...  1352   0.0    1
(BJ348772) Dictyostelium discoideum cDNA clone:dda34f12, 3' ...  1350   0.0    1
dna update 2008.11.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U82513_1(U82513|pid:none) Dictyostelium discoideum random slug c...   503   e-141
(Q55G11) RecName: Full=Neutral ceramidase B;          Short=N-CD...   384   e-105
EU200974_1(EU200974|pid:none) Triticum aestivum neutral ceramida...   186   2e-45
AC022464_28(AC022464|pid:none) Genomic sequence for Arabidopsis ...   186   2e-45
AP002819_24(AP002819|pid:none) Oryza sativa Japonica Group genom...   185   3e-45
AK099625_1(AK099625|pid:none) Oryza sativa Japonica Group cDNA c...   185   3e-45
AL450382_3(AL450382|pid:none) Human DNA sequence from clone RP11...   184   6e-45
BC167651_1(BC167651|pid:none) Xenopus tropicalis hypothetical pr...   184   8e-45
AL954360_4(AL954360|pid:none) Human DNA sequence from clone RP11...   183   1e-44
(Q304B9) RecName: Full=Neutral ceramidase;          Short=N-CDas...   181   4e-44
protein update 2009. 7.13
PSORT 

 psg: 0.96  gvh: 0.83  alm: 0.38  top: 0.17  tms: 0.00  mit: 0.45  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: extracellular, including cell wall
	 12.0 %: cytoplasmic
	 12.0 %: vacuolar
	 12.0 %: endoplasmic reticulum
	  8.0 %: mitochondrial
	  8.0 %: Golgi
	  4.0 %: nuclear

>> prediction for VHI396 is exc
5' end seq. ID VHI396F
5' end seq. 
>VHI396F.Seq
AAAAAGAATGAAAAGATCAATAGTATTTATTTATTCATTGGTAATTTTATTATTATCAGT
TGGGTTTATTGATGCATTCAAAATATCAATAGAAAATCATATAAAATTATCAGATGATTC
ATCTTATCAAATTGGTACAGGTATTTATGATATTACCGGACCAGGTGCAGAGACTAATAT
GATGGGCTATGCAATGCCAGGCCAAATTACTGGAGGAATTCATTTTAGACAACGTGCTAG
AGCATTTGTATTTATAGATTCAGAAGGTAATAGAGCAGTATATGTTAGTACTGATTCATG
TATGATTTTCCAAGAAGTTAAAATTCAAGTTATTCAAGATTTACAAGAAATTTTTGGACC
AACTTTATATACACATGATAATGTATTATTAAGCGGTACACATACTCATTCAGGTCCTGC
TGGTTTTTCAGAGTATGCATTATATGGTATTACAGCATTAGGTTTCTATAAAAAGAATTT
CGATACAATTTGTGATGGTATTGTTCAAGCAATTGTTAAAGCTCATAAATCGGTTCAACC
TGCAAGAATGTTAACTCAACANNNNNNNNNN
Length of 5' end seq. 571
3' end seq. ID VHI396Z
3' end seq. 
>VHI396Z.Seq
NNNNNNNNNNGGTCAAATTGTTTTAGTCGCTGTTCCAGGTGAATTCACAACAATGTCTGG
TAGAAGATTAAGAAATACCGTTAGAGAAATCATTGGTCAATCAATTGAAAATCCAATTGT
TTTAATTGCTGGTTTAGCAAATACTTATTCAGGTTATATTGCAACATTTGAAGAGTTTCA
AGTTCAAAGATATGAAGGTGCATCAACCGTTTTTGGACCTCATACTTTAGGAGCATATCA
ACAAGAATTTGCAAATCTTGCACAATCAATTGTAGATGGTAGTCAAGCTGATCCAGGAAC
ATTCCCAAGAAATATGTCTGGTCATACACCATTCTTTTTACCACCAGTCATTGTTGATGT
AGCTCCAAAATTCGACGATTTCGGTGATATCTATACTGATGTCTCTACAACTACACCATA
TTCAATCAATCAAACTGTTACCGTCATTTTCTATGGTGCCAATCTTAGAAATAATTTCAT
GACTGAATCTTCTTTCCTCACTGTTGATCAATTACAATCTAATGGTCAATGGACTACAAT
TTTAAATGATGGTGATTGGGATACAAAATTATATTGGAAAATGCATGATTTAGGTTTTTC
ACTCATCACTGTTGATTGGACAATCTCTCCAATCACTCAACCAGGTACTTATAGAATAAC
TCATAGTGGTTATGCTAAGAAAAATCCATTCTCTGATAATTTAACTTTTTATCAAGGTAT
TTCAAGTAATTTTAATGTTCAATAAAAATTAAAATAAATAAAAAAAGGATTTTTTTTT
Length of 3' end seq. 778
Connected seq. ID VHI396P
Connected seq. 
>VHI396P.Seq
AAAAAGAATGAAAAGATCAATAGTATTTATTTATTCATTGGTAATTTTATTATTATCAGT
TGGGTTTATTGATGCATTCAAAATATCAATAGAAAATCATATAAAATTATCAGATGATTC
ATCTTATCAAATTGGTACAGGTATTTATGATATTACCGGACCAGGTGCAGAGACTAATAT
GATGGGCTATGCAATGCCAGGCCAAATTACTGGAGGAATTCATTTTAGACAACGTGCTAG
AGCATTTGTATTTATAGATTCAGAAGGTAATAGAGCAGTATATGTTAGTACTGATTCATG
TATGATTTTCCAAGAAGTTAAAATTCAAGTTATTCAAGATTTACAAGAAATTTTTGGACC
AACTTTATATACACATGATAATGTATTATTAAGCGGTACACATACTCATTCAGGTCCTGC
TGGTTTTTCAGAGTATGCATTATATGGTATTACAGCATTAGGTTTCTATAAAAAGAATTT
CGATACAATTTGTGATGGTATTGTTCAAGCAATTGTTAAAGCTCATAAATCGGTTCAACC
TGCAAGAATGTTAACTCAACA----------GGTCAAATTGTTTTAGTCGCTGTTCCAGG
TGAATTCACAACAATGTCTGGTAGAAGATTAAGAAATACCGTTAGAGAAATCATTGGTCA
ATCAATTGAAAATCCAATTGTTTTAATTGCTGGTTTAGCAAATACTTATTCAGGTTATAT
TGCAACATTTGAAGAGTTTCAAGTTCAAAGATATGAAGGTGCATCAACCGTTTTTGGACC
TCATACTTTAGGAGCATATCAACAAGAATTTGCAAATCTTGCACAATCAATTGTAGATGG
TAGTCAAGCTGATCCAGGAACATTCCCAAGAAATATGTCTGGTCATACACCATTCTTTTT
ACCACCAGTCATTGTTGATGTAGCTCCAAAATTCGACGATTTCGGTGATATCTATACTGA
TGTCTCTACAACTACACCATATTCAATCAATCAAACTGTTACCGTCATTTTCTATGGTGC
CAATCTTAGAAATAATTTCATGACTGAATCTTCTTTCCTCACTGTTGATCAATTACAATC
TAATGGTCAATGGACTACAATTTTAAATGATGGTGATTGGGATACAAAATTATATTGGAA
AATGCATGATTTAGGTTTTTCACTCATCACTGTTGATTGGACAATCTCTCCAATCACTCA
ACCAGGTACTTATAGAATAACTCATAGTGGTTATGCTAAGAAAAATCCATTCTCTGATAA
TTTAACTTTTTATCAAGGTATTTCAAGTAATTTTAATGTTCAATAAAAATTAAAATAAAT
AAAAAAAGGATTTTTTTTT
Length of connected seq. 1329
Full length Seq ID -
Full length Seq. -
Length of full length seq. -