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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI379
Library VH
(Link to library)
Clone ID VHI379
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U12138-1
Original site URL
Representative seq. ID VHI379P
(Link to Original site)
Representative DNA sequence 
>VHI379 (VHI379Q) /CSM/VH/VHI3-D/VHI379Q.Seq.d/
ATAATTCAAATGATACAACATCATCCATTAATAGTACAACAGCAACAAACACAAACACAA
CAAATACAACCACAACAAATACAACAACAACAACAACAACAACAAATGGATTAAGTAATA
TTGTTAAACCACCACAAAGAGGTAAACATATAACATGGAGTTATGATGATATAATTGAAA
TTCATAAGAGAAGACATGTTTTAAAGAATAATGCAATAGAGATATTTTTAGGTAGTGGTG
TACCACATAAAACTTATTTATTTGCATTTAATAAACCATCAGATAGAGATATAGTTTATG
ATTTAATTATGTCAAAACCATTACCAAATCGTGTTGATTATGCAGCAGAGGTTCATGGTA
ACATTTTGAAGATGTCAATTACAAAGAAATGGCAATCTGGTTTAATTTCAAATTTCGAAT
ATTTAATGCATTTAAATACATTGGCAGGTCGTTCATTTAATGATTTAACACAGTATCCAA
TTTTCCCATTCATTCTAAGGGATTATGAATCAGAGGTATTGGATTTAGAGAATCCAAATA
CATTTAGAGATTTCACAAAACCAATGGGTGCTCAAGATCCAAAACGTTTAGAGAAATTCA
TAGAGAAATATAATGATCTATTAGAGATGAATGAAAAACCTTATCATTATGGTTCACATT
ATTXXXXXXXXXXTTGAATGATGATATCATCTGTGGTGACATTACAAAGAATGGTCGTTT
ATTCGTCACTGGTGGCACTGCTGGTACTGTTAAGGTTTGGAAACGTTGTAACAATGATGG
TACCATCATGACTAGAAAAGAGCGTGGTGATAATCTAAGTCTTTGGTCAACACTCTATGG
TCATACAAATTCTATACTTTGTGTAACCGTTAGTCAAGAGTATAGTATTATAGTTAGTGG
TTCAAAAGATTCAAATTGTATCATTTGGGATTTAAATAGATTAACTTATATCAATAGTTT
ACAACATGATCATCCTGTAACTTGTGTTCAAGTTTCTCCAACTTTTGGTTATATTGCAAC
TTTTGAAACCAATATATATAATAAACAAAATAATAATAGTGGTGGTGGTGGTTCAAAGAA
TGACAATAGTATTAATGGTTCAGGTTGTTTAAGACTTTGGAGTATTAATGGTACCTTACT
TGCAAAACAAAACTTTGTAAATGATCGTGTAAATTGCATGATCTTTACATCAACAATTCA
AGGTGTTAATACTAATCTTTTAATAACTGGTATGGAATCTGGAACTATCATACTTTGGAA
TGCTTGGAATCTTCAAAAAATTAGAACTTTAGTATCAAAATCTACAATCACTGCTTTAGC
CGTTAGTAAAGATAATACTCAATTAATTTCTGGTGATATTAATGGTCTCATTGAATGTTG
GTCAAGTCGTTCTTTNNAGGGTTATTCTTCAAT
sequence update 2002.10.25
Translated Amino Acid sequence 
NSNDTTSSINSTTATNTNTTNTTTTNTTTTTTTTNGLSNIVKPPQRGKHITWSYDDIIEI
HKRRHVLKNNAIEIFLGSGVPHKTYLFAFNKPSDRDIVYDLIMSKPLPNRVDYAAEVHGN
ILKMSITKKWQSGLISNFEYLMHLNTLAGRSFNDLTQYPIFPFILRDYESEVLDLENPNT
FRDFTKPMGAQDPKRLEKFIEKYNDLLEMNEKPYHYGSHY---

---LNDDIICGDITKNGRLFVTGGTAGTVKVWKRCNNDGTIMTRKERGDNLSLWSTLYGH
TNSILCVTVSQEYSIIVSGSKDSNCIIWDLNRLTYINSLQHDHPVTCVQVSPTFGYIATF
ETNIYNKQNNNSGGGGSKNDNSINGSGCLRLWSINGTLLAKQNFVNDRVNCMIFTSTIQG
VNTNLLITGMESGTIILWNAWNLQKIRTLVSKSTITALAVSKDNTQLISGDINGLIECWS
SRSXXGYSS
Translated Amino Acid sequence (All Frames) 
Frame A:
iiqmiqhhplivqqqqtqtqqiqpqqiqqqqqqqqmd*villnhhkevni*hgvmmi*lk
firedmf*rimq*ryf*vvvyhikliylhlinhqiei*fmi*lcqnhyqivlimqqrfmv
tf*rcqlqrngnlv*fqisni*ci*ihwqvvhlmi*hsiqfshsf*gimnqrywi*riqi
hleisqnqwvlkiqnv*rns*rnimiy*r*mknliimvhii---

---LNDDIICGDITKNGRLFVTGGTAGTVKVWKRCNNDGTIMTRKERGDNLSLWSTLYGH
TNSILCVTVSQEYSIIVSGSKDSNCIIWDLNRLTYINSLQHDHPVTCVQVSPTFGYIATF
ETNIYNKQNNNSGGGGSKNDNSINGSGCLRLWSINGTLLAKQNFVNDRVNCMIFTSTIQG
VNTNLLITGMESGTIILWNAWNLQKIRTLVSKSTITALAVSKDNTQLISGDINGLIECWS
SRSXXGYSS

Frame B:
*fk*yniih**ynsnkhkhnkynhnkynnnnnnnkwik*yc*tttkr*tynmel**yn*n
s*ektcfke*cnrdifr*wctt*nlfici**tir*rysl*fnyvktitksc*lcsrgsw*
hfedvnykemaiwfnfkfrifnafkyigrsfi**fntvsnfpihskgl*irgigfresky
i*rfhktngcsrsktfreihrei**sirde*ktlslwftl---

---*mmissvvtlqrmvvysslvallvllrfgnvvtmmvps*leksvvii*vfgqhsmvi
qilyfv*plvksivl*lvvqkiqivsfgi*id*lisivynmiil*lvfkflqllvilqll
kpiyiinkiiivvvvvqrmtivlmvqvv*dfgvlmvpylqnktl*miv*ia*slhqqfkv
lilif**lvwnlelsyfgmlgifkklel*yqnlqsll*plvkiiln*flvilmvslnvgq
vvlxrvilq

Frame C:
NSNDTTSSINSTTATNTNTTNTTTTNTTTTTTTTNGLSNIVKPPQRGKHITWSYDDIIEI
HKRRHVLKNNAIEIFLGSGVPHKTYLFAFNKPSDRDIVYDLIMSKPLPNRVDYAAEVHGN
ILKMSITKKWQSGLISNFEYLMHLNTLAGRSFNDLTQYPIFPFILRDYESEVLDLENPNT
FRDFTKPMGAQDPKRLEKFIEKYNDLLEMNEKPYHYGSHY---

---e**yhlw*hykewsfirhwwhcwyc*gletl*q*wyhhd*kraw**skslvntlwsy
kfytlcnr*srv*yys*wfkrfklyhlgfk*inlyq*ftt*sscnlcssfsnfwlycnf*
nqyi**tk***wwwwfke*q*y*wfrlfktley*wyltcktklck*scklhdlyinnsrc
*y*sfnnwygiwnyhtleclesskn*nfsikiynhcfsr**r*ysinfw*y*wsh*mlvk
sffxglffn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI379 (VHI379Q) /CSM/VH/VHI3-D/VHI379Q.Seq.d/                       2522   0.0
SLC545 (SLC545Q) /CSM/SL/SLC5-B/SLC545Q.Seq.d/                       1070   0.0
VSH731 (VSH731Q) /CSM/VS/VSH7-B/VSH731Q.Seq.d/                        133   8e-30
VSH718 (VSH718Q) /CSM/VS/VSH7-A/VSH718Q.Seq.d/                        131   3e-29
SSK807 (SSK807Q) /CSM/SS/SSK8-A/SSK807Q.Seq.d/                         48   4e-04
SHA515 (SHA515Q) /CSM/SH/SHA5-A/SHA515Q.Seq.d/                         48   4e-04
VHG141 (VHG141Q) /CSM/VH/VHG1-B/VHG141Q.Seq.d/                         46   0.001
VSF354 (VSF354Q) /CSM/VS/VSF3-C/VSF354Q.Seq.d/                         42   0.023
VSE519 (VSE519Q) /CSM/VS/VSE5-A/VSE519Q.Seq.d/                         42   0.023
SLG210 (SLG210Q) /CSM/SL/SLG2-A/SLG210Q.Seq.d/                         42   0.023
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ441805) Dictyostelium discoideum cDNA clone:ddv47n19, 3' ...  1455   0.0    1
(AY159038) Dictyostelium discoideum LvsB (lvsB) gene, comple...  1447   0.0    1
(AC116982) Dictyostelium discoideum chromosome 2 map 3622643...  1439   0.0    1
(BJ423056) Dictyostelium discoideum cDNA clone:ddv47n19, 5' ...  1106   0.0    1
(AU034302) Dictyostelium discoideum slug cDNA, clone SLC545.     1070   0.0    1
(AU060992) Dictyostelium discoideum slug cDNA, clone SLC545.      194   6e-45  1
(AU267885) Dictyostelium discoideum vegetative cDNA clone:VS...   133   2e-26  1
(AU267863) Dictyostelium discoideum vegetative cDNA clone:VS...   131   8e-26  1
(AF088979) Dictyostelium discoideum beige protein homolog (l...    90   6e-21  4
(AU267864) Dictyostelium discoideum vegetative cDNA clone:VS...   113   2e-20  1
dna update 2008.11.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC150246_1(BC150246|pid:none) Homo sapiens WD repeat and FYVE do...   158   4e-37
AK094910_1(AK094910|pid:none) Homo sapiens cDNA FLJ37591 fis, cl...   158   4e-37
(Q9Y2J7) RecName: Full=WD repeat and FYVE domain-containing prot...   158   4e-37
AB271946_1(AB271946|pid:none) Sus scrofa LYST mRNA for lysosomal...   157   9e-37
AK160397_1(AK160397|pid:none) Mus musculus adult male cerebellum...   157   9e-37
AL928828_1(AL928828|pid:none) Zebrafish DNA sequence from clone ...   157   9e-37
AL032675_1(AL032675|pid:none) Caenorhabditis elegans Plasmid VT2...   153   2e-35
AL032675_2(AL032675|pid:none) Caenorhabditis elegans Plasmid VT2...   153   2e-35
AE014134_1026(AE014134|pid:none) Drosophila melanogaster chromos...   152   2e-35
(Q99698) RecName: Full=Lysosomal-trafficking regulator; AltName:...   151   5e-35
protein update 2009. 7.13
PSORT 

 psg: 0.75  gvh: 0.30  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.53  mip: 0.08
 nuc: 0.05  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 76.0 %: mitochondrial
	 12.0 %: cytoplasmic
	 12.0 %: nuclear

>> prediction for VHI379 is mit
5' end seq. ID VHI379F
5' end seq. 
>VHI379F.Seq
ATAATTCAAATGATACAACATCATCCATTAATAGTACAACAGCAACAAACACAAACACAA
CAAATACAACCACAACAAATACAACAACAACAACAACAACAACAAATGGATTAAGTAATA
TTGTTAAACCACCACAAAGAGGTAAACATATAACATGGAGTTATGATGATATAATTGAAA
TTCATAAGAGAAGACATGTTTTAAAGAATAATGCAATAGAGATATTTTTAGGTAGTGGTG
TACCACATAAAACTTATTTATTTGCATTTAATAAACCATCAGATAGAGATATAGTTTATG
ATTTAATTATGTCAAAACCATTACCAAATCGTGTTGATTATGCAGCAGAGGTTCATGGTA
ACATTTTGAAGATGTCAATTACAAAGAAATGGCAATCTGGTTTAATTTCAAATTTCGAAT
ATTTAATGCATTTAAATACATTGGCAGGTCGTTCATTTAATGATTTAACACAGTATCCAA
TTTTCCCATTCATTCTAAGGGATTATGAATCAGAGGTATTGGATTTAGAGAATCCAAATA
CATTTAGAGATTTCACAAAACCAATGGGTGCTCAAGATCCAAAACGTTTAGAGAAATTCA
TAGAGAAATATAATGATCTATTAGAGATGAATGAAAAACCTTATCATTATGGTTCACATT
ATTNNNNNNNNNN
Length of 5' end seq. 673
3' end seq. ID VHI379Z
3' end seq. 
>VHI379Z.Seq
NNNNNNNNNNTTGAATGATGATATCATCTGTGGTGACATTACAAAGAATGGTCGTTTATT
CGTCACTGGTGGCACTGCTGGTACTGTTAAGGTTTGGAAACGTTGTAACAATGATGGTAC
CATCATGACTAGAAAAGAGCGTGGTGATAATCTAAGTCTTTGGTCAACACTCTATGGTCA
TACAAATTCTATACTTTGTGTAACCGTTAGTCAAGAGTATAGTATTATAGTTAGTGGTTC
AAAAGATTCAAATTGTATCATTTGGGATTTAAATAGATTAACTTATATCAATAGTTTACA
ACATGATCATCCTGTAACTTGTGTTCAAGTTTCTCCAACTTTTGGTTATATTGCAACTTT
TGAAACCAATATATATAATAAACAAAATAATAATAGTGGTGGTGGTGGTTCAAAGAATGA
CAATAGTATTAATGGTTCAGGTTGTTTAAGACTTTGGAGTATTAATGGTACCTTACTTGC
AAAACAAAACTTTGTAAATGATCGTGTAAATTGCATGATCTTTACATCAACAATTCAAGG
TGTTAATACTAATCTTTTAATAACTGGTATGGAATCTGGAACTATCATACTTTGGAATGC
TTGGAATCTTCAAAAAATTAGAACTTTAGTATCAAAATCTACAATCACTGCTTTAGCCGT
TAGTAAAGATAATACTCAATTAATTTCTGGTGATATTAATGGTCTCATTGAATGTTGGTC
AAGTCGTTCTTTNNAGGGTTATTCTTCAAT
Length of 3' end seq. 750
Connected seq. ID VHI379P
Connected seq. 
>VHI379P.Seq
ATAATTCAAATGATACAACATCATCCATTAATAGTACAACAGCAACAAACACAAACACAA
CAAATACAACCACAACAAATACAACAACAACAACAACAACAACAAATGGATTAAGTAATA
TTGTTAAACCACCACAAAGAGGTAAACATATAACATGGAGTTATGATGATATAATTGAAA
TTCATAAGAGAAGACATGTTTTAAAGAATAATGCAATAGAGATATTTTTAGGTAGTGGTG
TACCACATAAAACTTATTTATTTGCATTTAATAAACCATCAGATAGAGATATAGTTTATG
ATTTAATTATGTCAAAACCATTACCAAATCGTGTTGATTATGCAGCAGAGGTTCATGGTA
ACATTTTGAAGATGTCAATTACAAAGAAATGGCAATCTGGTTTAATTTCAAATTTCGAAT
ATTTAATGCATTTAAATACATTGGCAGGTCGTTCATTTAATGATTTAACACAGTATCCAA
TTTTCCCATTCATTCTAAGGGATTATGAATCAGAGGTATTGGATTTAGAGAATCCAAATA
CATTTAGAGATTTCACAAAACCAATGGGTGCTCAAGATCCAAAACGTTTAGAGAAATTCA
TAGAGAAATATAATGATCTATTAGAGATGAATGAAAAACCTTATCATTATGGTTCACATT
ATT----------TTGAATGATGATATCATCTGTGGTGACATTACAAAGAATGGTCGTTT
ATTCGTCACTGGTGGCACTGCTGGTACTGTTAAGGTTTGGAAACGTTGTAACAATGATGG
TACCATCATGACTAGAAAAGAGCGTGGTGATAATCTAAGTCTTTGGTCAACACTCTATGG
TCATACAAATTCTATACTTTGTGTAACCGTTAGTCAAGAGTATAGTATTATAGTTAGTGG
TTCAAAAGATTCAAATTGTATCATTTGGGATTTAAATAGATTAACTTATATCAATAGTTT
ACAACATGATCATCCTGTAACTTGTGTTCAAGTTTCTCCAACTTTTGGTTATATTGCAAC
TTTTGAAACCAATATATATAATAAACAAAATAATAATAGTGGTGGTGGTGGTTCAAAGAA
TGACAATAGTATTAATGGTTCAGGTTGTTTAAGACTTTGGAGTATTAATGGTACCTTACT
TGCAAAACAAAACTTTGTAAATGATCGTGTAAATTGCATGATCTTTACATCAACAATTCA
AGGTGTTAATACTAATCTTTTAATAACTGGTATGGAATCTGGAACTATCATACTTTGGAA
TGCTTGGAATCTTCAAAAAATTAGAACTTTAGTATCAAAATCTACAATCACTGCTTTAGC
CGTTAGTAAAGATAATACTCAATTAATTTCTGGTGATATTAATGGTCTCATTGAATGTTG
GTCAAGTCGTTCTTTNNAGGGTTATTCTTCAAT
Length of connected seq. 1403
Full length Seq ID -
Full length Seq. -
Length of full length seq. -