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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI375
Library VH
(Link to library)
Clone ID VHI375
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16251-1
Original site URL
Representative seq. ID VHI375P
(Link to Original site)
Representative DNA sequence 
>VHI375 (VHI375Q) /CSM/VH/VHI3-D/VHI375Q.Seq.d/
TTGGTACTTTTAGATACTTTTTAGAATTTCCATCATTGAATACCTTTTCAATTGATGTCG
ATAAAGATTCAATTGATAATCTTACATTATTCTTACGTTTAGTTGATCTATCTACAATTG
GTGATAGAGAAACTATTGTTAGAAATAATAAAATTTTTGTTCAAAAGATTTTCAAAGAGC
CAAAATTGTTATCACCATCAAATAATTACGAAAAGGATACAAATAATTTATATTTGAATA
CAAATAGTAATTTAGATTTTTCATTCCAATGCAAAGAGAAATTACCACATGGTTCCGTCG
AAATTAAAGTTATGTCAACTGGTATCAACTATAAAGATAATCTTTTCTATAGAGGTTTAT
TACCACAAGAAATCTTTACAAAAGGTGATATTTATTCTCCACCATTCGGTTTAGAATGTG
CTGGTTATATTACACGTGTTGCACCAAGTGGCGTTACTCGTTTCAAAGTTGGTGATCAAG
TTGTTGGTTTTGCAAGCCATTCTTTAAGTTCACTAGCTATTACTCATCAAGATAAAATTG
TATTAAAACCTGAAAATATTTCATTCAATGAAGCTGCTGCCGTTTGTGTAGTTTATGCAA
CAAGTTATTATTCAATTTTCCATATTGGTGCATTTATGGCCGATXXXXXXXXXXTTCATC
AATTGGTGCTATCATTGGTGGTTCAAAACAATGTGCATATAGTAGTGCTAATTTAGTATT
GGATTCATTATCAAACTATAGAAAGAGTATTGGTCTTGCTTCAACTTCAATTAATTGGGG
TGGTTTGGATGCTGGTGGTGTTGCCGCCACTGATAAATCAGTCGCAAGTTTCTTAGAGGG
TCAAGGTATTTTATTGGTTTCTTTATCAAAGATTTTAGGTTGTCTTGATTCAGTTTTCCA
ACCATCTAATTCCCATTTATCAAACTTTATGCTTTCAAGTTTCAATATTGATAATCTTTT
ATCTTCTGCACCTCAAATGAAGAGAAAAATGGGTCACCACCTAACAAATTATAAAACTTC
ATCTGCTAGCTCTGATGATTCATTGGGTGATTCTAGTTCTACTCAAGCAAAAGTCATTTC
TACAATCTCTGAATTATTATCAATTCATCCATCAAAATTAAATTTAGATACAAGATTAAA
AGACTATGGTATTGATTCTTTATTAACAGTACAATTAAAGAATTGGATAGATAAAGAATT
TACAAAGAATTTATTCACTCATTTACAATTATCATCAAGTAGTATTAATAGTATCATTCA
GAGAATTAGTTCAAAATCAACTTCAACATCTACTCCCAATCCAACTAACACCACAAAACA
AACCGCAACAACAAAAACATAAGTAGTTCAAACAACTCCAACTACATTNGACACTT
sequence update 2002.10.25
Translated Amino Acid sequence 
GTFRYFLEFPSLNTFSIDVDKDSIDNLTLFLRLVDLSTIGDRETIVRNNKIFVQKIFKEP
KLLSPSNNYEKDTNNLYLNTNSNLDFSFQCKEKLPHGSVEIKVMSTGINYKDNLFYRGLL
PQEIFTKGDIYSPPFGLECAGYITRVAPSGVTRFKVGDQVVGFASHSLSSLAITHQDKIV
LKPENISFNEAAAVCVVYATSYYSIFHIGAFMAD---

---SSIGAIIGGSKQCAYSSANLVLDSLSNYRKSIGLASTSINWGGLDAGGVAATDKSVA
SFLEGQGILLVSLSKILGCLDSVFQPSNSHLSNFMLSSFNIDNLLSSAPQMKRKMGHHLT
NYKTSSASSDDSLGDSSSTQAKVISTISELLSIHPSKLNLDTRLKDYGIDSLLTVQLKNW
IDKEFTKNLFTHLQLSSSSINSIIQRISSKSTSTSTPNPTNTTKQTATTKT*vvqttptt
xdt
Translated Amino Acid sequence (All Frames) 
Frame A:
lvlldtf*nfhh*ipfqlmsikiqliilhysyv*liylqlviekllleiikflfkrfsks
qncyhhqiitkriqiiyi*iqivi*ifhsnakrnyhmvpsklklcqlvstikiifsievy
yhkkslqkvifilhhsv*nvlvilhvlhqvallvsklvikllvlqail*vh*lllikikl
y*nlkifhsmkllpfv*fmqqviiqfsilvhlwp---

---finwcyhwwfktmci**c*fsigfiikl*keywscfnfn*lgwfgcwwccrh**isr
kflrgsryfigffikdfrls*fsfpti*fpfiklyafkfqy**sfifctsneekngsppn
kl*nfic*l**fig*f*fysskshfynl*iiinssikikfrykikrlwy*ffinstikel
dr*riykefihsftiiik*y**yhsen*fkinfniysqsn*hhktnrnnknisssnnsny
ixh

Frame B:
wyf*ilfrisiieylfn*cr*rfn**syiiltfs*siynw**rnyc*k**nfcskdfqra
kivitik*lrkgyk*fifeyk**frffipmqreittwfrrn*syvnwyql*r*sfl*rfi
ttrnlykr*ylfstirfrmcwlyytcctkwrysfqsw*sscwfckpffkftsyyssr*nc
ikt*kyfiq*sccrlcslcnkllfnfpywciygr---

---SSIGAIIGGSKQCAYSSANLVLDSLSNYRKSIGLASTSINWGGLDAGGVAATDKSVA
SFLEGQGILLVSLSKILGCLDSVFQPSNSHLSNFMLSSFNIDNLLSSAPQMKRKMGHHLT
NYKTSSASSDDSLGDSSSTQAKVISTISELLSIHPSKLNLDTRLKDYGIDSLLTVQLKNW
IDKEFTKNLFTHLQLSSSSINSIIQRISSKSTSTSTPNPTNTTKQTATTKT*vvqttptt
xdt

Frame C:
GTFRYFLEFPSLNTFSIDVDKDSIDNLTLFLRLVDLSTIGDRETIVRNNKIFVQKIFKEP
KLLSPSNNYEKDTNNLYLNTNSNLDFSFQCKEKLPHGSVEIKVMSTGINYKDNLFYRGLL
PQEIFTKGDIYSPPFGLECAGYITRVAPSGVTRFKVGDQVVGFASHSLSSLAITHQDKIV
LKPENISFNEAAAVCVVYATSYYSIFHIGAFMAD---

---hqlvlslvvqnnvhivvli*ywihyqtiervlvllqlqligvvwmlvvlpplinqsq
vs*rvkvfywflyqrf*vvliqfsnhlipiyqtlcfqvsiliifyllhlk*rekwvtt*q
iiklhllalmihwvilvllkqksflqslnyyqfihqn*i*iqd*ktmvlily*qyn*rig
*iknlqriysliynyhqvvlivsfrelvqnqlqhllpiqltpqnkpqqqkhk*fkqlqlh
xtl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI375 (VHI375Q) /CSM/VH/VHI3-D/VHI375Q.Seq.d/                       2662   0.0
VHO568 (VHO568Q) /CSM/VH/VHO5-C/VHO568Q.Seq.d/                       1415   0.0
VHO116 (VHO116Q) /CSM/VH/VHO1-A/VHO116Q.Seq.d/                       1415   0.0
VHN484 (VHN484Q) /CSM/VH/VHN4-D/VHN484Q.Seq.d/                       1415   0.0
VHK683 (VHK683Q) /CSM/VH/VHK6-D/VHK683Q.Seq.d/                       1415   0.0
VHJ243 (VHJ243Q) /CSM/VH/VHJ2-B/VHJ243Q.Seq.d/                       1415   0.0
VHI617 (VHI617Q) /CSM/VH/VHI6-A/VHI617Q.Seq.d/                       1415   0.0
VHI485 (VHI485Q) /CSM/VH/VHI4-D/VHI485Q.Seq.d/                       1415   0.0
VHH639 (VHH639Q) /CSM/VH/VHH6-B/VHH639Q.Seq.d/                       1415   0.0
VHD848 (VHD848Q) /CSM/VH/VHD8-B/VHD848Q.Seq.d/                       1415   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC116305) Dictyostelium discoideum chromosome 2 map 1005175...  1277   0.0    1
(BJ423018) Dictyostelium discoideum cDNA clone:ddv47f19, 5' ...  1277   0.0    1
(BJ446822) Dictyostelium discoideum cDNA clone:ddv63o20, 3' ...  1235   0.0    1
(BJ445768) Dictyostelium discoideum cDNA clone:ddv60g17, 3' ...  1235   0.0    1
(BJ445347) Dictyostelium discoideum cDNA clone:ddv59o03, 3' ...  1235   0.0    1
(BJ444912) Dictyostelium discoideum cDNA clone:ddv57h22, 3' ...  1235   0.0    1
(BJ444858) Dictyostelium discoideum cDNA clone:ddv57l17, 3' ...  1235   0.0    1
(BJ443979) Dictyostelium discoideum cDNA clone:ddv54f22, 3' ...  1235   0.0    1
(BJ443717) Dictyostelium discoideum cDNA clone:ddv53j22, 3' ...  1235   0.0    1
(BJ443397) Dictyostelium discoideum cDNA clone:ddv52e22, 3' ...  1235   0.0    1
dna update 2008.11.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q869W9) RecName: Full=Probable polyketide synthase 16;         ...   402   e-110
(Q869X2) RecName: Full=Probable polyketide synthase 17;         ...   372   e-101
AC116963_19(AC116963|pid:none) Dictyostelium discoideum chromoso...   145   3e-33
AC116963_16(AC116963|pid:none) Dictyostelium discoideum chromoso...   142   3e-32
(Q55DM7) RecName: Full=Probable polyketide synthase 2;          ...   135   3e-30
(Q54TW0) RecName: Full=Probable polyketide synthase 18;         ...   132   4e-29
(Q54B49) RecName: Full=Probable polyketide synthase 45;         ...   129   2e-28
AC117075_22(AC117075|pid:none) Dictyostelium discoideum chromoso...   127   1e-27
AC117176_58(AC117176|pid:none) Dictyostelium discoideum chromoso...   122   4e-26
(Q54ED6) RecName: Full=Probable polyketide synthase 41;         ...   120   9e-26
protein update 2009. 7.13
PSORT 

 psg: 0.83  gvh: 0.53  alm: 0.31  top: 0.50  tms: 0.07  mit: 0.33  mip: 0.02
 nuc: 0.04  erl: 0.00  erm: 0.80  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.04  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 40.0 %: mitochondrial
	 20.0 %: nuclear
	 16.0 %: cytoplasmic
	 12.0 %: Golgi
	  8.0 %: endoplasmic reticulum
	  4.0 %: vacuolar

>> prediction for VHI375 is mit
5' end seq. ID VHI375F
5' end seq. 
>VHI375F.Seq
TTGGTACTTTTAGATACTTTTTAGAATTTCCATCATTGAATACCTTTTCAATTGATGTCG
ATAAAGATTCAATTGATAATCTTACATTATTCTTACGTTTAGTTGATCTATCTACAATTG
GTGATAGAGAAACTATTGTTAGAAATAATAAAATTTTTGTTCAAAAGATTTTCAAAGAGC
CAAAATTGTTATCACCATCAAATAATTACGAAAAGGATACAAATAATTTATATTTGAATA
CAAATAGTAATTTAGATTTTTCATTCCAATGCAAAGAGAAATTACCACATGGTTCCGTCG
AAATTAAAGTTATGTCAACTGGTATCAACTATAAAGATAATCTTTTCTATAGAGGTTTAT
TACCACAAGAAATCTTTACAAAAGGTGATATTTATTCTCCACCATTCGGTTTAGAATGTG
CTGGTTATATTACACGTGTTGCACCAAGTGGCGTTACTCGTTTCAAAGTTGGTGATCAAG
TTGTTGGTTTTGCAAGCCATTCTTTAAGTTCACTAGCTATTACTCATCAAGATAAAATTG
TATTAAAACCTGAAAATATTTCATTCAATGAAGCTGCTGCCGTTTGTGTAGTTTATGCAA
CAAGTTATTATTCAATTTTCCATATTGGTGCATTTATGGCCGATNNNNNNNNNN
Length of 5' end seq. 654
3' end seq. ID VHI375Z
3' end seq. 
>VHI375Z.Seq
NNNNNNNNNNTTCATCAATTGGTGCTATCATTGGTGGTTCAAAACAATGTGCATATAGTA
GTGCTAATTTAGTATTGGATTCATTATCAAACTATAGAAAGAGTATTGGTCTTGCTTCAA
CTTCAATTAATTGGGGTGGTTTGGATGCTGGTGGTGTTGCCGCCACTGATAAATCAGTCG
CAAGTTTCTTAGAGGGTCAAGGTATTTTATTGGTTTCTTTATCAAAGATTTTAGGTTGTC
TTGATTCAGTTTTCCAACCATCTAATTCCCATTTATCAAACTTTATGCTTTCAAGTTTCA
ATATTGATAATCTTTTATCTTCTGCACCTCAAATGAAGAGAAAAATGGGTCACCACCTAA
CAAATTATAAAACTTCATCTGCTAGCTCTGATGATTCATTGGGTGATTCTAGTTCTACTC
AAGCAAAAGTCATTTCTACAATCTCTGAATTATTATCAATTCATCCATCAAAATTAAATT
TAGATACAAGATTAAAAGACTATGGTATTGATTCTTTATTAACAGTACAATTAAAGAATT
GGATAGATAAAGAATTTACAAAGAATTTATTCACTCATTTACAATTATCATCAAGTAGTA
TTAATAGTATCATTCAGAGAATTAGTTCAAAATCAACTTCAACATCTACTCCCAATCCAA
CTAACACCACAAAACAAACCGCAACAACAAAAACATAAGTAGTTCAAACAACTCCAACTA
CATTNGACACTT
Length of 3' end seq. 732
Connected seq. ID VHI375P
Connected seq. 
>VHI375P.Seq
TTGGTACTTTTAGATACTTTTTAGAATTTCCATCATTGAATACCTTTTCAATTGATGTCG
ATAAAGATTCAATTGATAATCTTACATTATTCTTACGTTTAGTTGATCTATCTACAATTG
GTGATAGAGAAACTATTGTTAGAAATAATAAAATTTTTGTTCAAAAGATTTTCAAAGAGC
CAAAATTGTTATCACCATCAAATAATTACGAAAAGGATACAAATAATTTATATTTGAATA
CAAATAGTAATTTAGATTTTTCATTCCAATGCAAAGAGAAATTACCACATGGTTCCGTCG
AAATTAAAGTTATGTCAACTGGTATCAACTATAAAGATAATCTTTTCTATAGAGGTTTAT
TACCACAAGAAATCTTTACAAAAGGTGATATTTATTCTCCACCATTCGGTTTAGAATGTG
CTGGTTATATTACACGTGTTGCACCAAGTGGCGTTACTCGTTTCAAAGTTGGTGATCAAG
TTGTTGGTTTTGCAAGCCATTCTTTAAGTTCACTAGCTATTACTCATCAAGATAAAATTG
TATTAAAACCTGAAAATATTTCATTCAATGAAGCTGCTGCCGTTTGTGTAGTTTATGCAA
CAAGTTATTATTCAATTTTCCATATTGGTGCATTTATGGCCGAT----------TTCATC
AATTGGTGCTATCATTGGTGGTTCAAAACAATGTGCATATAGTAGTGCTAATTTAGTATT
GGATTCATTATCAAACTATAGAAAGAGTATTGGTCTTGCTTCAACTTCAATTAATTGGGG
TGGTTTGGATGCTGGTGGTGTTGCCGCCACTGATAAATCAGTCGCAAGTTTCTTAGAGGG
TCAAGGTATTTTATTGGTTTCTTTATCAAAGATTTTAGGTTGTCTTGATTCAGTTTTCCA
ACCATCTAATTCCCATTTATCAAACTTTATGCTTTCAAGTTTCAATATTGATAATCTTTT
ATCTTCTGCACCTCAAATGAAGAGAAAAATGGGTCACCACCTAACAAATTATAAAACTTC
ATCTGCTAGCTCTGATGATTCATTGGGTGATTCTAGTTCTACTCAAGCAAAAGTCATTTC
TACAATCTCTGAATTATTATCAATTCATCCATCAAAATTAAATTTAGATACAAGATTAAA
AGACTATGGTATTGATTCTTTATTAACAGTACAATTAAAGAATTGGATAGATAAAGAATT
TACAAAGAATTTATTCACTCATTTACAATTATCATCAAGTAGTATTAATAGTATCATTCA
GAGAATTAGTTCAAAATCAACTTCAACATCTACTCCCAATCCAACTAACACCACAAAACA
AACCGCAACAACAAAAACATAAGTAGTTCAAACAACTCCAACTACATTNGACACTT
Length of connected seq. 1366
Full length Seq ID -
Full length Seq. -
Length of full length seq. -