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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI346
Library VH
(Link to library)
Clone ID VHI346
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VHI346P
(Link to Original site)
Representative DNA sequence 
>VHI346 (VHI346Q) /CSM/VH/VHI3-B/VHI346Q.Seq.d/
AATATCATTATTGTTAAAATAAGAGTAAATATTAAATAATTAAATATTAAAAAATGTCAT
CAATTGGTATTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTG
TTGAAATTATTGCAAATGATCAAGGTAATAGAACCACTCCATCTTATGTTGTATTTACTG
ATACAGAACGTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATA
CAGTATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTG
ATATGAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAG
TTGAATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCT
TAAAAATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTA
CCGTTCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTT
CAAAATTAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTX
XXXXXXXXXCTACATACTCTGATAATCAACCAGGTGTATTAATTCAAGTTTATGAAGGTG
AACGTGCTATGACCAAAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCAC
CAGCACCAAGAGGTGTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCT
TAAATGTATCAGCTGAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATG
ATAAAGGTCGTCTTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCA
AACAACAAGATGAACAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATG
CATTCACTGTTAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAG
ATAAATCAACTATTGAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAA
CCGCAGAAAAGGATGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAA
TCATGAGTAAACTCTATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTA
TGTCAAATGATTCACCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATT
TTTCAGAGGAAAACATCTCTATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAATC
AATAAAGCTTCTCATATAT
sequence update 2002.10.25
Translated Amino Acid sequence 
yhyc*nksky*iiky*KMSSIGIDLGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVVFTD
TERLIGDAAKNQVAMNPTNTVFDAKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQV
EFKGETKVFSPEEISSMVLLKMKETAEAYLGKTINNAVITVPAYFNDSQRQATKDAGTIS
KLNVQRIINEPTAAAIAYG---

---TYSDNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQVEVTFDVDANGIL
NVSAEDKSTGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDRVESKNKLENYA
FTVKNSIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKALEAVVNPI
MSKLYQEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisik*is*kiis**nq
*sfsy
Translated Amino Acid sequence (All Frames) 
Frame A:
niiivkirvnik*lnikkchqlvli*vqhihvlvfgkmivlkllqmikvieplhlmlyll
iqnv*lvmlqriklq*iqpiqylmlnv*lvvnlvtkkfkvi*nighsklfqrmvishifk
lnskvklkfshqkkslqwys*k*kkplklilvkpliml*lpfqlismivnvkqpkmlvqf
qn*mfnvllmnqllllllm---

---lhtliinqvy*fkfmkvnvl*pkiiiywvnlnflvfhqhqevyhklklhlmlmqmvs
*myqlkinqlvtnkrlqlpmikvvfqkkklkkwllmqrnsnnkmnnkkivlnqrinwkim
hsllkiqlkmkklqpkfqiqinqllnlklkvysng*nqikpqkrmnmkir*kp*kqlsiq
s*vnsikkevchkvvvcqvvcqmihqrvqttk*tn*idgifqrktslsnrflkk**vski
nkashi

Frame B:
islllk*e*ilnn*ilknvinwy*frynifmcwclak*sc*nyck*sr**nhsilcciy*
yrtfnw*cckesscnesnqysi*c*tfnws*i**qrssk*yetlaiqsyskgw**atyss
*iqr*n*sfltrrnlfngtlknernr*slsw*nh**ccnyrsslfq**stssnqrcwynf
kikcstyy**tncccyclw---

---yil**strcinssl*r*tcydqr**fig*i*tfwyststkrctts*syi*c*ckwyl
kcis*r*inw*qtkdynyq**rssfkrrn*kngc*creiqttr*ttkrsc*ike*igklc
ihc*kfn*r*kscsqnfrfr*iny*i*n*kctqmvriksnrrkg*i*r*deslrsscqsn
he*tlsrrryatrwwyarwyvk*ftkefkqqsrrirlteffrgkhlyqidflknnklvks
iklliy

Frame C:
yhyc*nksky*iiky*KMSSIGIDLGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVVFTD
TERLIGDAAKNQVAMNPTNTVFDAKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQV
EFKGETKVFSPEEISSMVLLKMKETAEAYLGKTINNAVITVPAYFNDSQRQATKDAGTIS
KLNVQRIINEPTAAAIAYG---

---TYSDNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQVEVTFDVDANGIL
NVSAEDKSTGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDRVESKNKLENYA
FTVKNSIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKALEAVVNPI
MSKLYQEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisik*is*kiis**nq
*sfsy
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI346 (VHI346Q) /CSM/VH/VHI3-B/VHI346Q.Seq.d/                       2486   0.0
VHQ260 (VHQ260Q) /CSM/VH/VHQ2-C/VHQ260Q.Seq.d/                       1447   0.0
VHP719 (VHP719Q) /CSM/VH/VHP7-A/VHP719Q.Seq.d/                       1447   0.0
VHP716 (VHP716Q) /CSM/VH/VHP7-A/VHP716Q.Seq.d/                       1447   0.0
VHP437 (VHP437Q) /CSM/VH/VHP4-B/VHP437Q.Seq.d/                       1447   0.0
VHP236 (VHP236Q) /CSM/VH/VHP2-B/VHP236Q.Seq.d/                       1447   0.0
VHO677 (VHO677Q) /CSM/VH/VHO6-D/VHO677Q.Seq.d/                       1447   0.0
VHO566 (VHO566Q) /CSM/VH/VHO5-C/VHO566Q.Seq.d/                       1447   0.0
VHN681 (VHN681Q) /CSM/VH/VHN6-D/VHN681Q.Seq.d/                       1447   0.0
VHK721 (VHK721Q) /CSM/VH/VHK7-A/VHK721Q.Seq.d/                       1447   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(X75263) D.discoideum (AX3) mRNA for heat shock cognate prot...  1447   0.0    1
(BJ446704) Dictyostelium discoideum cDNA clone:ddv63g16, 3' ...  1447   0.0    1
(BJ446275) Dictyostelium discoideum cDNA clone:ddv62p03, 3' ...  1447   0.0    1
(BJ446228) Dictyostelium discoideum cDNA clone:ddv62f05, 3' ...  1447   0.0    1
(BJ446016) Dictyostelium discoideum cDNA clone:ddv61j10, 3' ...  1447   0.0    1
(BJ446000) Dictyostelium discoideum cDNA clone:ddv61g10, 3' ...  1447   0.0    1
(BJ445856) Dictyostelium discoideum cDNA clone:ddv60i20, 3' ...  1447   0.0    1
(BJ445749) Dictyostelium discoideum cDNA clone:ddv60c17, 3' ...  1447   0.0    1
(BJ445191) Dictyostelium discoideum cDNA clone:ddv58a22, 3' ...  1447   0.0    1
(BJ443189) Dictyostelium discoideum cDNA clone:ddv52m03, 3' ...  1447   0.0    1
dna update 2008.11.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   358   2e-97
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   358   2e-97
(Q557E0) RecName: Full=Heat shock cognate 70 kDa protein 2;     ...   335   2e-90
AF025951_1(AF025951|pid:none) Dictyostelium discoideum heat-shoc...   335   2e-90
(Q54BE0) RecName: Full=Heat shock cognate 70 kDa protein 3;           317   6e-85
U71151_1(U71151|pid:none) Pneumocystis carinii heat shock protei...   313   9e-84
U80967_1(U80967|pid:none) Pneumocystis carinii f. sp. carinii he...   313   9e-84
AY495382_1(AY495382|pid:none) Trypanosoma rangeli isolate Tre he...   312   2e-83
(P11145) RecName: Full=Heat shock 70 kDa protein 4; AltName: Ful...   310   6e-83
FB790498_1(FB790498|pid:none) Sequence 9771 from Patent WO200803...   310   8e-83
protein update 2009. 7.13
PSORT 

 psg: 0.87  gvh: 0.35  alm: 0.46  top: 0.27  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VHI346 is cyt
5' end seq. ID VHI346F
5' end seq. 
>VHI346F.Seq
AATATCATTATTGTTAAAATAAGAGTAAATATTAAATAATTAAATATTAAAAAATGTCAT
CAATTGGTATTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTG
TTGAAATTATTGCAAATGATCAAGGTAATAGAACCACTCCATCTTATGTTGTATTTACTG
ATACAGAACGTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATA
CAGTATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTG
ATATGAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAG
TTGAATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCT
TAAAAATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTA
CCGTTCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTT
CAAAATTAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTN
NNNNNNNNN
Length of 5' end seq. 609
3' end seq. ID VHI346Z
3' end seq. 
>VHI346Z.Seq
NNNNNNNNNNCTACATACTCTGATAATCAACCAGGTGTATTAATTCAAGTTTATGAAGGT
GAACGTGCTATGACCAAAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCA
CCAGCACCAAGAGGTGTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATC
TTAAATGTATCAGCTGAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAAT
GATAAAGGTCGTCTTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTC
AAACAACAAGATGAACAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTAT
GCATTCACTGTTAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCA
GATAAATCAACTATTGAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAA
ACCGCAGAAAAGGATGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCA
ATCATGAGTAAACTCTATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGT
ATGTCAAATGATTCACCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAAT
TTTTCAGAGGAAAACATCTCTATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAAT
CAATAAAGCTTCTCATATAT
Length of 3' end seq. 740
Connected seq. ID VHI346P
Connected seq. 
>VHI346P.Seq
AATATCATTATTGTTAAAATAAGAGTAAATATTAAATAATTAAATATTAAAAAATGTCAT
CAATTGGTATTGATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTG
TTGAAATTATTGCAAATGATCAAGGTAATAGAACCACTCCATCTTATGTTGTATTTACTG
ATACAGAACGTTTAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATA
CAGTATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTG
ATATGAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAG
TTGAATTCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCT
TAAAAATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTA
CCGTTCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTT
CAAAATTAAATGTTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGT-
---------CTACATACTCTGATAATCAACCAGGTGTATTAATTCAAGTTTATGAAGGTG
AACGTGCTATGACCAAAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCAC
CAGCACCAAGAGGTGTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCT
TAAATGTATCAGCTGAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATG
ATAAAGGTCGTCTTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCA
AACAACAAGATGAACAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATG
CATTCACTGTTAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAG
ATAAATCAACTATTGAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAA
CCGCAGAAAAGGATGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAA
TCATGAGTAAACTCTATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTA
TGTCAAATGATTCACCAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATT
TTTCAGAGGAAAACATCTCTATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAATC
AATAAAGCTTCTCATATAT
Length of connected seq. 1329
Full length Seq ID -
Full length Seq. -
Length of full length seq. -