NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI325
Library VH
(Link to library)
Clone ID VHI325
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U10930-1
Original site URL
Representative seq. ID VHI325P
(Link to Original site)
Representative DNA sequence 
>VHI325 (VHI325Q) /CSM/VH/VHI3-B/VHI325Q.Seq.d/
ATCACAGATTATCATTCTTTTTTATTTTATTAATTTTTTTTTTATTTGTATATTTATAAA
GAAAAAGGAGGAAAATATATTGTATATATTTTTTGTANAATAAATAAATTATAGAAATGT
TATCATCAGCATTAAAATTAACTAAAAAAGTTTGCTCAACCAAATCAAATGGATTAATTA
NAAGTTTTTCAACACAAACTCAAAGTAGAGATTATGCAGTTGTANATCATACATATGATG
CAATTGTTGTTGGTGCAGGTGGTGCAGGTCTTAAAGCAGCCTTAGGTTTAACAGAAAAAG
GTTACAAGACAGCATGTATCACTAAATTATTCCCAACTCGTTCCCATACAGTCGCAGCAC
AAGGNGGTATTAATGCTGCTTTAGGAAATGCAGATCAAGATGATTGGANATGGCACGCAT
ATGATACAGTAAAGGGAAGTGATTTTTTAGGTGATCAANACGCCATTCATTACATGTGTA
AAGAAGCAGTTCCAACCGTTTTANAATTANAACAATATGGTGTACCATTCTCACGTATGG
ATGATGGTCGTATCTATCAAAGAGCATTCGGTGGTCAAAGTAAAAATTTTGGTAAAGGTG
XXXXXXXXXXTTGTGTCTCTGTCCATGGTGCCAATCGTTTAGGTGCCAACTCTCTATTGG
ATATCGTTGTCTTTGGTAGAGCTGTAGCCAATGAAATCGAAAATACCCTCGCCAAGGATA
CCCCACACAAACCATTACCACCAAATGNTGGTGAAGAATCAATTGCAAATATTGATGCCA
TTCGTTTCTCTAATGGTACTCGTTCANCCGCTGAAATTCGTTTAGAGATGCAAAAGATTA
TGCAAAGAAATGCTGCCGTCTTTAGAGATGGTCAAGTCTTAAAGGAAGGTGTTGAATTAA
TTGATAAATGTGCTCGTTCACTCATAAATGATCTCAAAACCACCGATCGTACCATGATTT
GGAATACCGATTTAATCGAATCATTAGAACTCCAAAATCTTATGACTCAAGCCGTCCTCA
ANTATGCACTNT
sequence update 2002.10.25
Translated Amino Acid sequence 
hrlsfffillifflfvyl*rkrrkiyciyflxnk*IIEMLSSALKLTKKVCSTKSNGLIX
SFSTQTQSRDYAVVXHTYDAIVVGAGGAGLKAALGLTEKGYKTACITKLFPTRSHTVAAQ
GGINAALGNADQDDWXWHAYDTVKGSDFLGDQXAIHYMCKEAVPTVLXLXQYGVPFSRMD
DGRIYQRAFGGQSKNFGKG---

---CVSVHGANRLGANSLLDIVVFGRAVANEIENTLAKDTPHKPLPPNXGEESIANIDAI
RFSNGTRSXAEIRLEMQKIMQRNAAVFRDGQVLKEGVELIDKCARSLINDLKTTDRTMIW
NTDLIESLELQNLMTQAVLXYAL
Translated Amino Acid sequence (All Frames) 
Frame A:
itdyhsflfy*fffylyiykekggkyivyifcxinkl*kcyhqh*n*lkkfaqpnqmd*l
xvfqhklkveimqlxiihmmqlllvqvvqvlkqp*v*qkkvtrqhvslnysqlvpiqsqh
kxvlmll*emqikmigxgthmiq*revif*vixtpfitcvkkqfqpfxnxnnmvyhshvw
mmvvsikehsvvkvkilvkv---

---lclcpwcqsfrcqlsigyrclw*scsq*nrkyprqgyptqtittkxw*rincky*ch
sfl*wysfxr*nsfrdakdyakkccrl*rwsslkgrc*in**mcsfthk*sqnhrsyhdl
eyrfnriirtpksydssrpqxct

Frame B:
sqiiilfyfinffficifikkkeenilyiffvx*inyrnviisikin*ksllnqikwinx
kffntnsk*rlcscxsyi*cnccwcrwcrs*sslrfnrkrlqdsmyh*iipnsfpysrst
rxy*ccfrkcrsr*lxmari*yskgk*ffr*sxrhslhv*rsssnrfxixtiwctiltyg
*wsylsksirwsk*kfw*r---

---CVSVHGANRLGANSLLDIVVFGRAVANEIENTLAKDTPHKPLPPNXGEESIANIDAI
RFSNGTRSXAEIRLEMQKIMQRNAAVFRDGQVLKEGVELIDKCARSLINDLKTTDRTMIW
NTDLIESLELQNLMTQAVLXYAL

Frame C:
hrlsfffillifflfvyl*rkrrkiyciyflxnk*IIEMLSSALKLTKKVCSTKSNGLIX
SFSTQTQSRDYAVVXHTYDAIVVGAGGAGLKAALGLTEKGYKTACITKLFPTRSHTVAAQ
GGINAALGNADQDDWXWHAYDTVKGSDFLGDQXAIHYMCKEAVPTVLXLXQYGVPFSRMD
DGRIYQRAFGGQSKNFGKG---

---vslsmvpiv*vptlywislslvel*pmkskipspriphtnhyhqmxvknqlqilmpf
vslmvlvxplkfv*rckrlckemlpslemvks*rkvln*linvlvhs*miskppivp*fg
ipi*snh*nskil*lkpssxmhx
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI325 (VHI325Q) /CSM/VH/VHI3-B/VHI325Q.Seq.d/                       1832   0.0
AFN559 (AFN559Q) /CSM/AF/AFN5-C/AFN559Q.Seq.d/                        975   0.0
AFK828 (AFK828Q) /CSM/AF/AFK8-B/AFK828Q.Seq.d/                        868   0.0
VHI140 (VHI140Q) /CSM/VH/VHI1-B/VHI140Q.Seq.d/                        841   0.0
SFK519 (SFK519Q) /CSM/SF/SFK5-A/SFK519Q.Seq.d/                        803   0.0
VHP153 (VHP153Q) /CSM/VH/VHP1-C/VHP153Q.Seq.d/                        801   0.0
VFK559 (VFK559Q) /CSM/VF/VFK5-C/VFK559Q.Seq.d/                        801   0.0
AHD463 (AHD463Q) /CSM/AH/AHD4-C/AHD463Q.Seq.d/                        801   0.0
AFL443 (AFL443Q) /CSM/AF/AFL4-B/AFL443Q.Seq.d/                        801   0.0
AFI160 (AFI160Q) /CSM/AF/AFI1-C/AFI160Q.Seq.d/                        801   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AF211482) Dictyostelium discoideum SdhA (sdhA) gene, comple...   904   0.0    3
(BJ422852) Dictyostelium discoideum cDNA clone:ddv47b07, 5' ...   912   0.0    2
(BJ328535) Dictyostelium discoideum cDNA clone:dda28e15, 5' ...   904   0.0    1
(BJ441606) Dictyostelium discoideum cDNA clone:ddv47b07, 3' ...   815   0.0    1
(BJ446084) Dictyostelium discoideum cDNA clone:ddv61i13, 3' ...   801   0.0    1
(BJ433656) Dictyostelium discoideum cDNA clone:ddv22e15, 3' ...   801   0.0    1
(BJ401069) Dictyostelium discoideum cDNA clone:dds22e05, 3' ...   801   0.0    1
(BJ390333) Dictyostelium discoideum cDNA clone:dds22e05, 5' ...   801   0.0    1
(BJ349798) Dictyostelium discoideum cDNA clone:dda37n16, 3' ...   801   0.0    1
(BJ345703) Dictyostelium discoideum cDNA clone:dda28e15, 3' ...   801   0.0    1
dna update 2008.11.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CR954214_12(CR954214|pid:none) Ostreococcus tauri strain OTTH059...   189   1e-46
CP001331_489(CP001331|pid:none) Micromonas sp. RCC299 chromosome...   187   5e-46
(O82663) RecName: Full=Succinate dehydrogenase [ubiquinone] flav...   186   9e-46
EU970939_1(EU970939|pid:none) Zea mays clone 354307 succinate de...   185   2e-45
BT056014_1(BT056014|pid:none) Zea mays full-length cDNA clone ZM...   185   2e-45
CR382137_303(CR382137|pid:none) Debaryomyces hansenii strain CBS...   184   4e-45
FN392322_764(FN392322|pid:none) Pichia pastoris GS115 chromosome...   183   7e-45
CP000600_8(CP000600|pid:none) Ostreococcus lucimarinus CCE9901 c...   183   7e-45
(Q9UTJ7) RecName: Full=Probable succinate dehydrogenase [ubiquin...   182   1e-44
FJ217746_1(FJ217746|pid:none) Botryotinia fuckeliana isolate I1 ...   182   1e-44
protein update 2009. 7.13
PSORT 

 psg: 0.68  gvh: 0.56  alm: 0.35  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.06
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 24.0 %: mitochondrial
	 20.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system

>> prediction for VHI325 is nuc
5' end seq. ID VHI325F
5' end seq. 
>VHI325F.Seq
ATCACAGATTATCATTCTTTTTTATTTTATTAATTTTTTTTTTATTTGTATATTTATAAA
GAAAAAGGAGGAAAATATATTGTATATATTTTTTGTANAATAAATAAATTATAGAAATGT
TATCATCAGCATTAAAATTAACTAAAAAAGTTTGCTCAACCAAATCAAATGGATTAATTA
NAAGTTTTTCAACACAAACTCAAAGTAGAGATTATGCAGTTGTANATCATACATATGATG
CAATTGTTGTTGGTGCAGGTGGTGCAGGTCTTAAAGCAGCCTTAGGTTTAACAGAAAAAG
GTTACAAGACAGCATGTATCACTAAATTATTCCCAACTCGTTCCCATACAGTCGCAGCAC
AAGGNGGTATTAATGCTGCTTTAGGAAATGCAGATCAAGATGATTGGANATGGCACGCAT
ATGATACAGTAAAGGGAAGTGATTTTTTAGGTGATCAANACGCCATTCATTACATGTGTA
AAGAAGCAGTTCCAACCGTTTTANAATTANAACAATATGGTGTACCATTCTCACGTATGG
ATGATGGTCGTATCTATCAAAGAGCATTCGGTGGTCAAAGTAAAAATTTTGGTAAAGGTG
NNNNNNNNNN
Length of 5' end seq. 610
3' end seq. ID VHI325Z
3' end seq. 
>VHI325Z.Seq
NNNNNNNNNNTTGTGTCTCTGTCCATGGTGCCAATCGTTTAGGTGCCAACTCTCTATTGG
ATATCGTTGTCTTTGGTAGAGCTGTAGCCAATGAAATCGAAAATACCCTCGCCAAGGATA
CCCCACACAAACCATTACCACCAAATGNTGGTGAAGAATCAATTGCAAATATTGATGCCA
TTCGTTTCTCTAATGGTACTCGTTCANCCGCTGAAATTCGTTTAGAGATGCAAAAGATTA
TGCAAAGAAATGCTGCCGTCTTTAGAGATGGTCAAGTCTTAAAGGAAGGTGTTGAATTAA
TTGATAAATGTGCTCGTTCACTCATAAATGATCTCAAAACCACCGATCGTACCATGATTT
GGAATACCGATTTAATCGAATCATTAGAACTCCAAAATCTTATGACTCAAGCCGTCCTCA
ANTATGCACTNT
Length of 3' end seq. 432
Connected seq. ID VHI325P
Connected seq. 
>VHI325P.Seq
ATCACAGATTATCATTCTTTTTTATTTTATTAATTTTTTTTTTATTTGTATATTTATAAA
GAAAAAGGAGGAAAATATATTGTATATATTTTTTGTANAATAAATAAATTATAGAAATGT
TATCATCAGCATTAAAATTAACTAAAAAAGTTTGCTCAACCAAATCAAATGGATTAATTA
NAAGTTTTTCAACACAAACTCAAAGTAGAGATTATGCAGTTGTANATCATACATATGATG
CAATTGTTGTTGGTGCAGGTGGTGCAGGTCTTAAAGCAGCCTTAGGTTTAACAGAAAAAG
GTTACAAGACAGCATGTATCACTAAATTATTCCCAACTCGTTCCCATACAGTCGCAGCAC
AAGGNGGTATTAATGCTGCTTTAGGAAATGCAGATCAAGATGATTGGANATGGCACGCAT
ATGATACAGTAAAGGGAAGTGATTTTTTAGGTGATCAANACGCCATTCATTACATGTGTA
AAGAAGCAGTTCCAACCGTTTTANAATTANAACAATATGGTGTACCATTCTCACGTATGG
ATGATGGTCGTATCTATCAAAGAGCATTCGGTGGTCAAAGTAAAAATTTTGGTAAAGGTG
----------TTGTGTCTCTGTCCATGGTGCCAATCGTTTAGGTGCCAACTCTCTATTGG
ATATCGTTGTCTTTGGTAGAGCTGTAGCCAATGAAATCGAAAATACCCTCGCCAAGGATA
CCCCACACAAACCATTACCACCAAATGNTGGTGAAGAATCAATTGCAAATATTGATGCCA
TTCGTTTCTCTAATGGTACTCGTTCANCCGCTGAAATTCGTTTAGAGATGCAAAAGATTA
TGCAAAGAAATGCTGCCGTCTTTAGAGATGGTCAAGTCTTAAAGGAAGGTGTTGAATTAA
TTGATAAATGTGCTCGTTCACTCATAAATGATCTCAAAACCACCGATCGTACCATGATTT
GGAATACCGATTTAATCGAATCATTAGAACTCCAAAATCTTATGACTCAAGCCGTCCTCA
ANTATGCACTNT
Length of connected seq. 1022
Full length Seq ID -
Full length Seq. -
Length of full length seq. -