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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI301
Library VH
(Link to library)
Clone ID VHI301
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11299-1
Original site URL
Representative seq. ID VHI301P
(Link to Original site)
Representative DNA sequence 
>VHI301 (VHI301Q) /CSM/VH/VHI3-A/VHI301Q.Seq.d/
AAAGTTTTTCAAAATTAAAGAAAAAAAGAAAATCCAAAAGAAAAAGAAGAATAAATTATT
AACAGCAAGTTTAACACCATCGTCATCGAGTTCAACTGCTGCAATTCTATCATCTATTCA
AACAACTGGTGGACCAATTCCAGTTGCTCATTCAAAATCTTCACCATTGGTTGCGACAAA
ACACCAAAGAAGATCATCATTATCGACATCGACACCGGGATTAAATTCAATTTTAAAAGC
CCATACTAAACCACCCATCTTAGGTGGTAGTAATAGTAATAGTAGTAATAATATCGTTGG
TTTAGGTTCTTCATCCATATCACCATTGAATGGTCATCATGGTACTGGTATTCAACATTT
AAATGCTATTAAAGATAAATTAGCTCATCAACAACAAAAGCATTTACAACATAGAATTCA
CTTACATCAACAAGCTCAACAACAAGCACAACAACAACAAGCACACCAACCACAACAAGA
ATTAAAATTATTCCCAACAACTAGTATTCCTGATCAAATTCCAGTTACCGCCACTGGACA
ACCATCAACACCAACTATTAAATTAACAAGTGTTGATTCAACACCAGAGTTTGAATCACC
AAXXXXXXXXXXCCTTACAAGATTCTATTCGCTTGCAATGATGGTGGTATTCTAGATAGT
GATCATAAGGCCATACCAGTGGTATATATGGATCAAGATTATCAAACCTTGATAGAGGCC
GACCATACAACCAATCGTCGTAGGGTTCAATTGAAACAAATGAAAAGAATCGTTGATAAT
TTACCATCATCCACAACGGTTGTCGTCACTTCCATTGAAAATGTCACACATCAATTGTTG
TTCCCTCGTTTAGCCAATGGTACTATCATTAAAAAGTCTGAAGAGAACAAAGTATTAATA
TTTGATAATACAAATTATCAAAACATCGATATGGATAGACTCAAAGAATTGATTGAAAAT
AGTTTTGGAAAAATGTTATCCACCGATTATTTCGATTACTTGAAAGATTCCCTCTATAGA
TTATATCTTTGTGTTTCATCCGATGGTGAATACAATGGTTGTGCAATCGTAACTTTTAAA
GAACCAGATTATATTCCTTATCTTTGTAAGTTTTGTGTGAAAAAGAGTGCTCAAGGTTTG
GGTTTAGGTGATCTCGTTTGGAGAGCTCTTCAAAGAGATAATGAGAAACTCTATTGGAGA
TCAAGAAATAGTAATCCATTAAATCCTTGGTATTTCCAAAGATGTCAAGGTAGCTTTAGA
GCTGATGAATCTTTCACTATCTTTTGGTATGGTGATGATAAATTTGAAGATGGTAGTTTC
TNGGTGAAACGCTTTAAGTAAACCAGTTACAATATTCCAAATCCAACAGTTTCATAATA
sequence update 2002.10.25
Translated Amino Acid sequence 
KFFKIKEKKKIQKKKKNKLLTASLTPSSSSSTAAILSSIQTTGGPIPVAHSKSSPLVATK
HQRRSSLSTSTPGLNSILKAHTKPPILGGSNSNSSNNIVGLGSSSISPLNGHHGTGIQHL
NAIKDKLAHQQQKHLQHRIHLHQQAQQQAQQQQAHQPQQELKLFPTTSIPDQIPVTATGQ
PSTPTIKLTSVDSTPEFESP---

---PYKILFACNDGGILDSDHKAIPVVYMDQDYQTLIEADHTTNRRRVQLKQMKRIVDNL
PSSTTVVVTSIENVTHQLLFPRLANGTIIKKSEENKVLIFDNTNYQNIDMDRLKELIENS
FGKMLSTDYFDYLKDSLYRLYLCVSSDGEYNGCAIVTFKEPDYIPYLCKFCVKKSAQGLG
LGDLVWRALQRDNEKLYWRSRNSNPLNPWYFQRCQGSFRADESFTIFWYGDDKFEDGSFX
VKRFK*tsynipnptvs*
Translated Amino Acid sequence (All Frames) 
Frame A:
kvfqn*rkkenpkekee*iinskfntiviefnccnsiiysnnwwtnsscsfkiftigcdk
tpkkiiiididtgikfnfkspy*tthlrw********yrwfrffihitiewsswywystf
kcy*r*isssttkaftt*nsltstssttsttttstptttrikiipnn*ys*snssyrhwt
tintny*inkc*fntrv*it---

---PYKILFACNDGGILDSDHKAIPVVYMDQDYQTLIEADHTTNRRRVQLKQMKRIVDNL
PSSTTVVVTSIENVTHQLLFPRLANGTIIKKSEENKVLIFDNTNYQNIDMDRLKELIENS
FGKMLSTDYFDYLKDSLYRLYLCVSSDGEYNGCAIVTFKEPDYIPYLCKFCVKKSAQGLG
LGDLVWRALQRDNEKLYWRSRNSNPLNPWYFQRCQGSFRADESFTIFWYGDDKFEDGSFX
VKRFK*tsynipnptvs*

Frame B:
KFFKIKEKKKIQKKKKNKLLTASLTPSSSSSTAAILSSIQTTGGPIPVAHSKSSPLVATK
HQRRSSLSTSTPGLNSILKAHTKPPILGGSNSNSSNNIVGLGSSSISPLNGHHGTGIQHL
NAIKDKLAHQQQKHLQHRIHLHQQAQQQAQQQQAHQPQQELKLFPTTSIPDQIPVTATGQ
PSTPTIKLTSVDSTPEFESP---

---ltrfyslammvvf*iviirpyqwyiwikiikp**rptiqpivvgfn*nk*kesliiy
hhpqrlsslplkmshinccslv*pmvlslkslkrtky*yliiqiiktsiwidskn*lkiv
lekcyppiisit*kipsidyifvfhpmvntmvvqs*llknqiiflifvsfv*krvlkvwv
*visfgelfkeimrnsigdqeivih*ilgiskdvkvalelmnlslsfgmvminlkmvvsx
*nalskpvtifqiqqfhn

Frame C:
sfsklkkkrkskrkrriny*qqv*hhrhrvqllqfyhlfkqlvdqfqlliqnlhhwlrqn
tkedhhyrhrhrd*iqf*kpilnhps*vvvivivviislv*vlhpyhh*mvimvlvfni*
mllkin*linnksiynieftyinklnnkhnnnkhtnhnkn*nysqqlvflikfqlppldn
hqhqlln*qvliqhqslnhq---

---lqdsirlq*wwysr**s*ghtsgiygsrlsnldrgrpynqss*gsietneknr**ft
iihngcrhfh*kchtsivvpsfsqwyyh*kv*reqsini**yklskhryg*tqrid*k*f
wknvihrlfrllerfpl*iislcfirw*iqwlcnrnf*rtrlyslsl*vlcekecsrfgf
r*srlessskr**etlleikk**sikslvfpkmsr*l*s**ifhyllvw***i*rw*flg
etl*vnqlqysksnsfii
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI301 (VHI301Q) /CSM/VH/VHI3-A/VHI301Q.Seq.d/                       1721   0.0
SHA640 (SHA640Q) /CSM/SH/SHA6-B/SHA640Q.Seq.d/                       1465   0.0
AHE495 (AHE495Q) /CSM/AH/AHE4-D/AHE495Q.Seq.d/                       1439   0.0
AHI363 (AHI363Q) /CSM/AH/AHI3-C/AHI363Q.Seq.d/                       1229   0.0
VHO884 (VHO884Q) /CSM/VH/VHO8-D/VHO884Q.Seq.d/                       1225   0.0
VHF395 (VHF395Q) /CSM/VH/VHF3-D/VHF395Q.Seq.d/                       1084   0.0
VHA549 (VHA549Q) /CSM/VH/VHA5-C/VHA549Q.Seq.d/                        737   0.0
VHO716 (VHO716Q) /CSM/VH/VHO7-A/VHO716Q.Seq.d/                         52   2e-05
SHA547 (SHA547Q) /CSM/SH/SHA5-B/SHA547Q.Seq.d/                         52   2e-05
VHP643 (VHP643Q) /CSM/VH/VHP6-B/VHP643Q.Seq.d/                         46   0.001
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ441532) Dictyostelium discoideum cDNA clone:ddv47b01, 3' ...  1515   0.0    1
(BJ403536) Dictyostelium discoideum cDNA clone:dds25o10, 3' ...  1400   0.0    3
(BJ350462) Dictyostelium discoideum cDNA clone:dda39n24, 3' ...  1354   0.0    2
(AC116986) Dictyostelium discoideum chromosome 2 map 2234041...  1257   0.0    4
(BJ445852) Dictyostelium discoideum cDNA clone:ddv60h22, 3' ...  1160   0.0    3
(BJ352722) Dictyostelium discoideum cDNA clone:dda47n15, 3' ...  1142   0.0    4
(BJ439879) Dictyostelium discoideum cDNA clone:ddv41n23, 3' ...  1063   0.0    2
(BJ422770) Dictyostelium discoideum cDNA clone:ddv47b01, 5' ...   664   0.0    2
(BJ334756) Dictyostelium discoideum cDNA clone:dda47n15, 5' ...   648   0.0    2
(BJ332676) Dictyostelium discoideum cDNA clone:dda39n24, 5' ...   664   0.0    2
dna update 2008.11.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM746676_73(AM746676|pid:none) Sorangium cellulosum 'So ce 56' c...   165   4e-39
(Q8N159) RecName: Full=N-acetylglutamate synthase, mitochondrial...   144   8e-33
AY158070_1(AY158070|pid:none) Homo sapiens N-acetylglutamate syn...   144   8e-33
EF429095_1(EF429095|pid:none) Sus scrofa N-acetylglutamate synth...   142   4e-32
CP001111_2721(CP001111|pid:none) Stenotrophomonas maltophilia R5...   139   3e-31
AF462069_1(AF462069|pid:none) Mus musculus N-acetylglutamate syn...   137   9e-31
AK075765_1(AK075765|pid:none) Mus musculus adult male testis cDN...   137   9e-31
AP008229_2518(AP008229|pid:none) Xanthomonas oryzae pv. oryzae M...   137   1e-30
AE013598_2617(AE013598|pid:none) Xanthomonas oryzae pv. oryzae K...   137   1e-30
AM039952_2547(AM039952|pid:none) Xanthomonas campestris pv. vesi...   137   1e-30
protein update 2009. 7.13
PSORT 

 psg: 0.24  gvh: 0.61  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.50  mip: 0.22
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	 20.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal

>> prediction for VHI301 is cyt
5' end seq. ID VHI301F
5' end seq. 
>VHI301F.Seq
AAAGTTTTTCAAAATTAAAGAAAAAAAGAAAATCCAAAAGAAAAAGAAGAATAAATTATT
AACAGCAAGTTTAACACCATCGTCATCGAGTTCAACTGCTGCAATTCTATCATCTATTCA
AACAACTGGTGGACCAATTCCAGTTGCTCATTCAAAATCTTCACCATTGGTTGCGACAAA
ACACCAAAGAAGATCATCATTATCGACATCGACACCGGGATTAAATTCAATTTTAAAAGC
CCATACTAAACCACCCATCTTAGGTGGTAGTAATAGTAATAGTAGTAATAATATCGTTGG
TTTAGGTTCTTCATCCATATCACCATTGAATGGTCATCATGGTACTGGTATTCAACATTT
AAATGCTATTAAAGATAAATTAGCTCATCAACAACAAAAGCATTTACAACATAGAATTCA
CTTACATCAACAAGCTCAACAACAAGCACAACAACAACAAGCACACCAACCACAACAAGA
ATTAAAATTATTCCCAACAACTAGTATTCCTGATCAAATTCCAGTTACCGCCACTGGACA
ACCATCAACACCAACTATTAAATTAACAAGTGTTGATTCAACACCAGAGTTTGAATCACC
AANNNNNNNNNN
Length of 5' end seq. 612
3' end seq. ID VHI301Z
3' end seq. 
>VHI301Z.Seq
NNNNNNNNNNCCTTACAAGATTCTATTCGCTTGCAATGATGGTGGTATTCTAGATAGTGA
TCATAAGGCCATACCAGTGGTATATATGGATCAAGATTATCAAACCTTGATAGAGGCCGA
CCATACAACCAATCGTCGTAGGGTTCAATTGAAACAAATGAAAAGAATCGTTGATAATTT
ACCATCATCCACAACGGTTGTCGTCACTTCCATTGAAAATGTCACACATCAATTGTTGTT
CCCTCGTTTAGCCAATGGTACTATCATTAAAAAGTCTGAAGAGAACAAAGTATTAATATT
TGATAATACAAATTATCAAAACATCGATATGGATAGACTCAAAGAATTGATTGAAAATAG
TTTTGGAAAAATGTTATCCACCGATTATTTCGATTACTTGAAAGATTCCCTCTATAGATT
ATATCTTTGTGTTTCATCCGATGGTGAATACAATGGTTGTGCAATCGTAACTTTTAAAGA
ACCAGATTATATTCCTTATCTTTGTAAGTTTTGTGTGAAAAAGAGTGCTCAAGGTTTGGG
TTTAGGTGATCTCGTTTGGAGAGCTCTTCAAAGAGATAATGAGAAACTCTATTGGAGATC
AAGAAATAGTAATCCATTAAATCCTTGGTATTTCCAAAGATGTCAAGGTAGCTTTAGAGC
TGATGAATCTTTCACTATCTTTTGGTATGGTGATGATAAATTTGAAGATGGTAGTTTCTN
GGTGAAACGCTTTAAGTAAACCAGTTACAATATTCCAAATCCAACAGTTTCATAATA
Length of 3' end seq. 777
Connected seq. ID VHI301P
Connected seq. 
>VHI301P.Seq
AAAGTTTTTCAAAATTAAAGAAAAAAAGAAAATCCAAAAGAAAAAGAAGAATAAATTATT
AACAGCAAGTTTAACACCATCGTCATCGAGTTCAACTGCTGCAATTCTATCATCTATTCA
AACAACTGGTGGACCAATTCCAGTTGCTCATTCAAAATCTTCACCATTGGTTGCGACAAA
ACACCAAAGAAGATCATCATTATCGACATCGACACCGGGATTAAATTCAATTTTAAAAGC
CCATACTAAACCACCCATCTTAGGTGGTAGTAATAGTAATAGTAGTAATAATATCGTTGG
TTTAGGTTCTTCATCCATATCACCATTGAATGGTCATCATGGTACTGGTATTCAACATTT
AAATGCTATTAAAGATAAATTAGCTCATCAACAACAAAAGCATTTACAACATAGAATTCA
CTTACATCAACAAGCTCAACAACAAGCACAACAACAACAAGCACACCAACCACAACAAGA
ATTAAAATTATTCCCAACAACTAGTATTCCTGATCAAATTCCAGTTACCGCCACTGGACA
ACCATCAACACCAACTATTAAATTAACAAGTGTTGATTCAACACCAGAGTTTGAATCACC
AA----------CCTTACAAGATTCTATTCGCTTGCAATGATGGTGGTATTCTAGATAGT
GATCATAAGGCCATACCAGTGGTATATATGGATCAAGATTATCAAACCTTGATAGAGGCC
GACCATACAACCAATCGTCGTAGGGTTCAATTGAAACAAATGAAAAGAATCGTTGATAAT
TTACCATCATCCACAACGGTTGTCGTCACTTCCATTGAAAATGTCACACATCAATTGTTG
TTCCCTCGTTTAGCCAATGGTACTATCATTAAAAAGTCTGAAGAGAACAAAGTATTAATA
TTTGATAATACAAATTATCAAAACATCGATATGGATAGACTCAAAGAATTGATTGAAAAT
AGTTTTGGAAAAATGTTATCCACCGATTATTTCGATTACTTGAAAGATTCCCTCTATAGA
TTATATCTTTGTGTTTCATCCGATGGTGAATACAATGGTTGTGCAATCGTAACTTTTAAA
GAACCAGATTATATTCCTTATCTTTGTAAGTTTTGTGTGAAAAAGAGTGCTCAAGGTTTG
GGTTTAGGTGATCTCGTTTGGAGAGCTCTTCAAAGAGATAATGAGAAACTCTATTGGAGA
TCAAGAAATAGTAATCCATTAAATCCTTGGTATTTCCAAAGATGTCAAGGTAGCTTTAGA
GCTGATGAATCTTTCACTATCTTTTGGTATGGTGATGATAAATTTGAAGATGGTAGTTTC
TNGGTGAAACGCTTTAAGTAAACCAGTTACAATATTCCAAATCCAACAGTTTCATAATA
Length of connected seq. 1369
Full length Seq ID -
Full length Seq. -
Length of full length seq. -