NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI283
Library VH
(Link to library)
Clone ID VHI283
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16171-1
Original site URL
Representative seq. ID VHI283E
(Link to Original site)
Representative DNA sequence 
>VHI283 (VHI283Q) /CSM/VH/VHI2-D/VHI283Q.Seq.d/
ACAACTCTAATATATATATATAATAAAATGAATAAGTTAACAATTTTATTCTTAATTTTG
GCTTTAATTTCAGTCATTTATGCTCAACACAAATTCCCATCATCATCTGAAGATTCATCA
TCAAATGATTCAAATAGCCAAGTAACTGGTTCACAATCATACTCTGGTTCACAATCCGAT
TCAAACTCAGGATCAGAATCCCACACAATTAACACCGGTTCATCATATTCTGGTTCTGGT
TCTGGTTCAAGTGGTATTTCAGGCGGTTCTGGTTCAGGTTCAGGTTCAGGTTCAGGCTCA
GGTAGCGGTTCAGGTTCAGGTTCAGGTGCTGTCAGTGGTTCACAATCAGGTTCAGGTGCT
GTCAGTGGTTCACAATCAGGTTCAGGTGCTGTTAGTGGTTCACAATCTGGTGTCCAAACT
GGTTCACAATCAGGTGCTGGTAGTGCTTCAGGTGCTTTCACTGGTAACCCATCTGGTTCT
CAATCTCAAGAAATTAATACTGGTTCATCATACAGTGGTAGTGGTAGTGGTGCCCCAACT
GGTGCCGCTACTGGTTCAGGTTCAGGTTCAGGTTCATCAGGAACAGTTTACTATTAAATA
AATTTTAAATTATAACGAA
sequence update 2002.10.27
Translated Amino Acid sequence 
TTLIYIYNKMNKLTILFLILALISVIYAQHKFPSSSEDSSSNDSNSQVTGSQSYSGSQSD
SNSGSESHTINTGSSYSGSGSGSSGISGGSGSGSGSGSGSGSGSGSGSGAVSGSQSGSGA
VSGSQSGSGAVSGSQSGVQTGSQSGAGSASGAFTGNPSGSQSQEINTGSSYSGSGSGAPT
GAATGSGSGSGSSGTVYY*infkl*r
Translated Amino Acid sequence (All Frames) 
Frame A:
TTLIYIYNKMNKLTILFLILALISVIYAQHKFPSSSEDSSSNDSNSQVTGSQSYSGSQSD
SNSGSESHTINTGSSYSGSGSGSSGISGGSGSGSGSGSGSGSGSGSGSGAVSGSQSGSGA
VSGSQSGSGAVSGSQSGVQTGSQSGAGSASGAFTGNPSGSQSQEINTGSSYSGSGSGAPT
GAATGSGSGSGSSGTVYY*infkl*r


Frame B:
ql*yiyiik*is*qfys*fwl*fqsfmlntnshhhlkihhqmiqiak*lvhnhtlvhnpi
qtqdqnptqltpvhhilvlvlvqvvfqavlvqvqvqvqaqvavqvqvqvlsvvhnqvqvl
svvhnqvqvllvvhnlvsklvhnqvlvvlqvlslvthlvlnlkklilvhhtvvvvvvpql
vpllvqvqvqvhqeqftik*ilnyne


Frame C:
nsniyi**ne*vnnfilnfgfnfshlcstqipiii*rfiik*fk*psnwftiilwftirf
klririphn*hrfiifwfwfwfkwyfrrfwfrfrfrfrlr*rfrfrfrccqwftirfrcc
qwftirfrcc*wftiwcpnwftircw*cfrcfhw*piwfsisrn*ywfiiqw*w*wcpnw
crywfrfrfrfirnslllnkf*iit
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSK169 (VSK169Q) /CSM/VS/VSK1-C/VSK169Q.Seq.d/                        579   e-164
VSG609 (VSG609Q) /CSM/VS/VSG6-A/VSG609Q.Seq.d/                        579   e-164
VSD364 (VSD364Q) /CSM/VS/VSD3-C/VSD364Q.Seq.d/                        579   e-164
VHQ154 (VHQ154Q) /CSM/VH/VHQ1-C/VHQ154Q.Seq.d/                        579   e-164
VHJ422 (VHJ422Q) /CSM/VH/VHJ4-A/VHJ422Q.Seq.d/                        579   e-164
VHI283 (VHI283Q) /CSM/VH/VHI2-D/VHI283Q.Seq.d/                        579   e-164
VFA868 (VFA868Q) /CSM/VF/VFA8-C/VFA868Q.Seq.d/                        579   e-164
SLF225 (SLF225Q) /CSM/SL/SLF2-B/SLF225Q.Seq.d/                        579   e-164
VSC419 (VSC419Q) /CSM/VS/VSC4-A/VSC419Q.Seq.d/                        551   e-156
VFB716 (VFB716Q) /CSM/VF/VFB7-A/VFB716Q.Seq.d/                        537   e-152
own update 2002.12.31
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ441765) Dictyostelium discoideum cDNA clone:ddv47e22, 3' ...   490   0.0    2
(BJ446721) Dictyostelium discoideum cDNA clone:ddv63k13, 3' ...   490   0.0    2
(BJ442208) Dictyostelium discoideum cDNA clone:ddv49l06, 3' ...   490   0.0    2
(BJ429612) Dictyostelium discoideum cDNA clone:ddv4p03, 3' e...   398   0.0    3
(AU270748) Dictyostelium discoideum vegetative cDNA clone:VS...   424   0.0    2
(AU270749) Dictyostelium discoideum vegetative cDNA clone:VS...   490   0.0    2
(AU266248) Dictyostelium discoideum vegetative cDNA clone:VS...   406   0.0    2
(BJ423015) Dictyostelium discoideum cDNA clone:ddv47e22, 5' ...   406   0.0    2
(BJ427796) Dictyostelium discoideum cDNA clone:ddv63k13, 5' ...   406   0.0    2
(BJ423426) Dictyostelium discoideum cDNA clone:ddv49l06, 5' ...   406   0.0    2
dna update 2008.11.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54T37) RecName: Full=Uncharacterized transmembrane protein DDB...    64   3e-09
CP000393_2258(CP000393|pid:none) Trichodesmium erythraeum IMS101...    49   1e-04
CP000828_875(CP000828|pid:none) Acaryochloris marina MBIC11017, ...    47   3e-04
BA000004_971(BA000004|pid:none) Bacillus halodurans C-125 DNA, c...    47   3e-04
CP001325_267(CP001325|pid:none) Micromonas sp. RCC299 chromosome...    46   0.001
U00051_3(U00051|pid:none) Caenorhabditis elegans cosmid F42G9, c...    45   0.002
U00051_4(U00051|pid:none) Caenorhabditis elegans cosmid F42G9, c...    45   0.002
AM407005_1(AM407005|pid:none) Staphylococcus aureus partial clfB...    45   0.002
AM407050_1(AM407050|pid:none) Staphylococcus aureus partial clfB...    45   0.002
AM407093_1(AM407093|pid:none) Staphylococcus aureus partial clfB...    45   0.002
protein update 2009. 7.13
PSORT 

 psg: 0.79  gvh: 0.84  alm: 0.22  top: 0.23  tms: 0.07  mit: 0.33  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.77  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: cytoplasmic
	 20.0 %: Golgi
	 20.0 %: endoplasmic reticulum
	 16.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for VHI283 is cyt
5' end seq. ID VHI283F
5' end seq. 
>VHI283F.Seq
ACAACTCTAATATATATATATAATAAAATGAATAAGTTAACAATTTTATTCTTAATTTTG
GCTTTAATTTCAGTCATTTATGCTCAACACAAATTCCCATCATCATCTGAAGATTCATCA
TCAAATGATTCAAATAGCCAAGTAACTGGTTCACAATCATACTCTGGTTCACAATCCGAT
TCAAACTCAGGATCAGAATCCCACACAATTAACACCGGTTCATCATATTCTGGTTCTGGT
TCTGGTTCAAGTGGTATTTCAGGCGGTTCTGGTTCAGGTTCAGGTTCAGGTTCAGGCTCA
GGTAGCGGTTCAGGTTCAGGTTCAGGTGCTGTCAGTGGTTCACAATCAGGTTCAGGTGCT
GTCAGTGGTTCACAATCAGGTTCAGGTGCTGTTAGTGGTTCACAATCTGGTGTCCAAACT
GGTTCACAATCAGGTGCTGGTAGTGCTTCAGGTGCTTTCACTGGTAACCCATCTGGTTCT
CAATCTCAAGAAATTAATACTGGTTCATCATACAGTGGTAGTGGTAGTGGTGCCCCAACT
GGTGCCGCTACTGGTTCAGGTTCAGGTTCAGGTTCANNNNNNNNNN
Length of 5' end seq. 586
3' end seq. ID VHI283Z
3' end seq. 
>VHI283Z.Seq
NNNNNNNNNNACAACTCTAATATATATATATAATAAAATGAATAAGTTAACAATTTTATT
CTTAATTTTGGCTTTAATTTCAGTCATTTATGCTCAACACAAATTCCCATCATCATCTGA
AGATTCATCATCAAATGATTCAAATAGCCAAGTAACTGGTTCACAATCATACTCTGGTTC
ACAATCCGATTCAAACTCAGGATCAGAATCCCACACAATTAACACCGGTTCATCATATTC
TGGTTCTGGTTCTGGTTCAAGTGGTATTTCAGGCGGTTCTGGTTCAGGTTCAGGTTCAGG
TTCAGGCTCAGGTAGCGGTTCAGGTTCAGGTTCAGGTGCTGTCAGTGGTTCACAATCAGG
TTCAGGTGCTGTCAGTGGTTCACAATCAGGTTCAGGTGCTGTTAGTGGTTCACAATCTGG
TGTCCAAACTGGTTCACAATCAGGTGCTGGTAGTGCTTCAGGTGCTTTCACTGGTAACCC
ATCTGGTTCTCAATCTCAAGAAATTAATACTGGTTCATCATACAGTGGTAGTGGTAGTGG
TGCCCCAACTGGTGCCGCTACTGGTTCAGGTTCAGGTTCAGGTTCATCAGGAACAGTTTA
CTATTAAATAAATTTTAAATTATAACGAA
Length of 3' end seq. 629
Connected seq. ID VHI283P
Connected seq. 
>VHI283P.Seq
ACAACTCTAATATATATATATAATAAAATGAATAAGTTAACAATTTTATTCTTAATTTTG
GCTTTAATTTCAGTCATTTATGCTCAACACAAATTCCCATCATCATCTGAAGATTCATCA
TCAAATGATTCAAATAGCCAAGTAACTGGTTCACAATCATACTCTGGTTCACAATCCGAT
TCAAACTCAGGATCAGAATCCCACACAATTAACACCGGTTCATCATATTCTGGTTCTGGT
TCTGGTTCAAGTGGTATTTCAGGCGGTTCTGGTTCAGGTTCAGGTTCAGGTTCAGGCTCA
GGTAGCGGTTCAGGTTCAGGTTCAGGTGCTGTCAGTGGTTCACAATCAGGTTCAGGTGCT
GTCAGTGGTTCACAATCAGGTTCAGGTGCTGTTAGTGGTTCACAATCTGGTGTCCAAACT
GGTTCACAATCAGGTGCTGGTAGTGCTTCAGGTGCTTTCACTGGTAACCCATCTGGTTCT
CAATCTCAAGAAATTAATACTGGTTCATCATACAGTGGTAGTGGTAGTGGTGCCCCAACT
GGTGCCGCTACTGGTTCAGGTTCAGGTTCAGGTTCA----------ACAACTCTAATATA
TATATATAATAAAATGAATAAGTTAACAATTTTATTCTTAATTTTGGCTTTAATTTCAGT
CATTTATGCTCAACACAAATTCCCATCATCATCTGAAGATTCATCATCAAATGATTCAAA
TAGCCAAGTAACTGGTTCACAATCATACTCTGGTTCACAATCCGATTCAAACTCAGGATC
AGAATCCCACACAATTAACACCGGTTCATCATATTCTGGTTCTGGTTCTGGTTCAAGTGG
TATTTCAGGCGGTTCTGGTTCAGGTTCAGGTTCAGGTTCAGGCTCAGGTAGCGGTTCAGG
TTCAGGTTCAGGTGCTGTCAGTGGTTCACAATCAGGTTCAGGTGCTGTCAGTGGTTCACA
ATCAGGTTCAGGTGCTGTTAGTGGTTCACAATCTGGTGTCCAAACTGGTTCACAATCAGG
TGCTGGTAGTGCTTCAGGTGCTTTCACTGGTAACCCATCTGGTTCTCAATCTCAAGAAAT
TAATACTGGTTCATCATACAGTGGTAGTGGTAGTGGTGCCCCAACTGGTGCCGCTACTGG
TTCAGGTTCAGGTTCAGGTTCATCAGGAACAGTTTACTATTAAATAAATTTTAAATTATA
ACGAA
Length of connected seq. 1195
Full length Seq ID VHI283E
Full length Seq. 
>VHI283E.Seq
ACAACTCTAATATATATATATAATAAAATGAATAAGTTAACAATTTTATTCTTAATTTTG
GCTTTAATTTCAGTCATTTATGCTCAACACAAATTCCCATCATCATCTGAAGATTCATCA
TCAAATGATTCAAATAGCCAAGTAACTGGTTCACAATCATACTCTGGTTCACAATCCGAT
TCAAACTCAGGATCAGAATCCCACACAATTAACACCGGTTCATCATATTCTGGTTCTGGT
TCTGGTTCAAGTGGTATTTCAGGCGGTTCTGGTTCAGGTTCAGGTTCAGGTTCAGGCTCA
GGTAGCGGTTCAGGTTCAGGTTCAGGTGCTGTCAGTGGTTCACAATCAGGTTCAGGTGCT
GTCAGTGGTTCACAATCAGGTTCAGGTGCTGTTAGTGGTTCACAATCTGGTGTCCAAACT
GGTTCACAATCAGGTGCTGGTAGTGCTTCAGGTGCTTTCACTGGTAACCCATCTGGTTCT
CAATCTCAAGAAATTAATACTGGTTCATCATACAGTGGTAGTGGTAGTGGTGCCCCAACT
GGTGCCGCTACTGGTTCAGGTTCAGGTTCAGGTTCATCAGGAACAGTTTACTATTAAATA
AATTTTAAATTATAACGAA
Length of full length seq. 619