>VHI281 (VHI281Q) /CSM/VH/VHI2-D/VHI281Q.Seq.d/
XXXXXXXXXXATTCAAGTAACCAAATACAGATAAAGATGGTATTATAACAAAATCAAATA
ATGTCATTTGGAAATATAGTAAAGATAAATTAGCAGAATCCTATAAATACACCAATAAAG
TTGATGGTGTTGAATTATGTTTCACCGATTCATCAATGAATATTGCAAACATTTCATTGA
ATGGTAGTAAATCAGATGGTCCATTTTCAAATGCTTATTATAACAAATACTTCTTTGGTG
ATAATTCAATTGCAAGATTATTCACTGATCAATCCTATTTGAATCAATTTACAATTTCAA
CCACCTATACAAACATTGATGGATTAAATTTAACTACATTATCAAAAGAAAGTGATAATT
CAAATGTTTATTACTTCCCAGCCGGTTGGAGTTTCGAAACTTTTGATACCACAAACACTG
ACCTTTCCACTCCATTATTAATTACAAAGGTAAATAATGGTTCAACTGAAACTTGGAGAA
TCATTAAATATGATAACACTGTCGATAGTGATTTATACTCTAAATGTCAACCACCAAAGA
AAGGAGGAATTCATGTTAATATCTCCAAAAATGGTATCATTGCCGTTGTCGTCATTGTTG
TCGTTGTTATTATTGGTGTTGGTGCTATAG

---fk*PNTDKDGIITKSNNVIWKYSKDKLAESYKYTNKVDGVELCFTDSSMNIANISLN
GSKSDGPFSNAYYNKYFFGDNSIARLFTDQSYLNQFTISTTYTNIDGLNLTTLSKESDNS
NVYYFPAGWSFETFDTTNTDLSTPLLITKVNNGSTETWRIIKYDNTVDSDLYSKCQPPKK
GGIHVNISKNGIIAVVVIVVVVIIGVGAI

Frame A:
---fk*PNTDKDGIITKSNNVIWKYSKDKLAESYKYTNKVDGVELCFTDSSMNIANISLN
GSKSDGPFSNAYYNKYFFGDNSIARLFTDQSYLNQFTISTTYTNIDGLNLTTLSKESDNS
NVYYFPAGWSFETFDTTNTDLSTPLLITKVNNGSTETWRIIKYDNTVDSDLYSKCQPPKK
GGIHVNISKNGIIAVVVIVVVVIIGVGAI


Frame B:
---ssnqiqikmvl*qnqimsfgnivkin*qnpintpiklmvlnyvspihq*ilqtfh*m
vvnqmvhfqmliitntslviiqlqdyslinpi*inlqfqppiqtlmd*i*lhyqkkviiq
mfitsqpvgvskllipqtltfplhy*lqr*imvqlklgeslnmitlsiviytlnvnhqrk
eefmlispkmvslplssllsllllvlvl*


Frame C:
---qvtkyr*rwyynkik*chlei**r*isril*ihq*s*wc*imfhrfineyckhfiew
**irwsifkcll*qillw**fnckiih*silfesiynfnhlykh*wikfnyiikrk**fk
clllpsrlefrnf*yhkh*pfhsiinykgk*wfn*nlenh*i**hcr**fil*msttker
rnsc*ylqkwyhcrcrhccrcyywcwcy

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI281 (VHI281Q) /CSM/VH/VHI2-D/VHI281Q.Seq.d/                       1229   0.0
VHN487 (VHN487Q) /CSM/VH/VHN4-D/VHN487Q.Seq.d/                       1209   0.0
VHJ180 (VHJ180Q) /CSM/VH/VHJ1-D/VHJ180Q.Seq.d/                       1209   0.0
AHO295 (AHO295Q) /CSM/AH/AHO2-D/AHO295Q.Seq.d/                       1209   0.0
VHP795 (VHP795Q) /CSM/VH/VHP7-D/VHP795Q.Seq.d/                       1201   0.0
VFC253 (VFC253Q) /CSM/VF/VFC2-C/VFC253Q.Seq.d/                       1187   0.0
VHK874 (VHK874Q) /CSM/VH/VHK8-D/VHK874Q.Seq.d/                       1174   0.0
VHE114 (VHE114Q) /CSM/VH/VHE1-A/VHE114Q.Seq.d/                       1148   0.0
AFL483 (AFL483Q) /CSM/AF/AFL4-D/AFL483Q.Seq.d/                       1140   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC117075|AC117075.2 Dictyostelium discoideum chromosome 2 map 5201047-5455781 strain AX4, complete sequence.    34   9e-06  16
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.    42   2e-05  12
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    44   3e-04  10
BD309267|BD309267.1 Drug targets in Candida albicans.              56   6e-04  1
AX489330|AX489330.1 Sequence 6630 from Patent WO02053728.          56   6e-04  1
AL929353|AL929353.1 Plasmodium falciparum strain 3D7, chromosome 5, segment 3/4.    42   0.002  8
AL844506|AL844506.1 Plasmodium falciparum chromosome  7.           34   0.008  16
AC136652|AC136652.1 Rattus norvegicus clone CH230-359N16, *** SEQUENCING IN PROGRESS ***, 46 unordered pieces.    52   0.010  1
AC116988|AC116988.2 Dictyostelium discoideum chromosome 2 map 6445720-6776760 strain AX4, complete sequence.    34   0.012  12
AC115599|AC115599.2 Dictyostelium discoideum chromosome 2 map 4229098-4354721 strain AX4, complete sequence.    34   0.016  10

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116957_18(AC116957|pid:none) Dictyostelium discoideum chromoso...    40   0.042
CP000408_2109(CP000408|pid:none) Streptococcus suis 98HAH33, com...    39   0.094
AL034559_46(AL034559|pid:none) Plasmodium falciparum MAL3P7, com...    38   0.21
AE014188_55(AE014188|pid:none) Plasmodium falciparum 3D7 chromos...    38   0.21
AK304309_1(AK304309|pid:none) Homo sapiens cDNA FLJ54663 complet...    34   3.0
AE017198_392(AE017198|pid:none) Lactobacillus johnsonii NCC 533,...    34   3.0
BX640635_1(BX640635|pid:none) Homo sapiens mRNA; cDNA DKFZp779L0...    34   3.0
BVEBCZ(A28181) cysZ protein - Salmonella typhimurium  &M21450_1(...    34   3.0
AF516672_1(AF516672|pid:none) Homo sapiens putative non-ribosoma...    34   3.0
AY422212_1(AY422212|pid:none) Homo sapiens putative non-ribosoma...    34   3.0

 psg: 0.64  gvh: 0.29  alm: 0.06  top: 0.30  tms: 0.07  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.03  gpi: 0.00  myr: 0.00
 dna: 0.12  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 1.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: cytoplasmic
	 20.0 %: nuclear
	 16.0 %: vesicles of secretory system
	 12.0 %: endoplasmic reticulum
	 12.0 %: plasma membrane
	  8.0 %: Golgi
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHI281 is cyt

>VHI281Z.Seq
NNNNNNNNNNATTCAAGTAACCAAATACAGATAAAGATGGTATTATAACAAAATCAAATA
ATGTCATTTGGAAATATAGTAAAGATAAATTAGCAGAATCCTATAAATACACCAATAAAG
TTGATGGTGTTGAATTATGTTTCACCGATTCATCAATGAATATTGCAAACATTTCATTGA
ATGGTAGTAAATCAGATGGTCCATTTTCAAATGCTTATTATAACAAATACTTCTTTGGTG
ATAATTCAATTGCAAGATTATTCACTGATCAATCCTATTTGAATCAATTTACAATTTCAA
CCACCTATACAAACATTGATGGATTAAATTTAACTACATTATCAAAAGAAAGTGATAATT
CAAATGTTTATTACTTCCCAGCCGGTTGGAGTTTCGAAACTTTTGATACCACAAACACTG
ACCTTTCCACTCCATTATTAATTACAAAGGTAAATAATGGTTCAACTGAAACTTGGAGAA
TCATTAAATATGATAACACTGTCGATAGTGATTTATACTCTAAATGTCAACCACCAAAGA
AAGGAGGAATTCATGTTAATATCTCCAAAAATGGTATCATTGCCGTTGTCGTCATTGTTG
TCGTTGTTATTATTGGTGTTGGTGCTATAG