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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI269
Library VH
(Link to library)
Clone ID VHI269
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHI269P
(Link to Original site)
Representative DNA sequence 
>VHI269 (VHI269Q) /CSM/VH/VHI2-C/VHI269Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATCTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAGTCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTXXXX
XXXXXXCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATAT
TTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTT
GTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACT
GATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCT
GCTGAATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCC
CCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATT
GGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTTATTTACCAGTATTA
GAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAA
TGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAA
GTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAA
TTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTNAGTTTTCAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHLDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVG---

---PEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLH
TDAIHRGGGQIIPTARRVLYAAELTASPTLLEPIYLVEITAPENAIGGIYSVLNRRRGIV
IGEERRIGSPLFSVKAYLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKAT
EVALATRKRKGLAPEIPDLDKFHEKL*ti*valxfs
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllisitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplslhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsll---

---PEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLH
TDAIHRGGGQIIPTARRVLYAAELTASPTLLEPIYLVEITAPENAIGGIYSVLNRRRGIV
IGEERRIGSPLFSVKAYLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKAT
EVALATRKRKGLAPEIPDLDKFHEKL*ti*valxfs

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycssrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*vficitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw---

---qkvtvpisssmspkvfni*mklkilslvlsngppkkvlfvmkt*evsvsifmmspst
lmpstevvvkssqllvvysmlln*lppqls*nqst**kllpqktllvvftlysivvevll
lvknvvsvphyslskliyqy*npsvspliyvptlpvklshnvssiiglqlvlstkirkpl
ksl*pperekv*pqksqiltnstknskqfkshxsfq

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHLDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVG---

---rr*rcqsprqchqrcsife*n*rffrwcfpmghqrrccl**kherypfqsl*chpph
*chpqrwwsnhpncssctlcc*inclpnslrtnllsrnycprkrywwyllctqss*rycy
w*rtsyrfpiilcqslftsirilrfhr*ftfphcrssfptmclrslgfnwccqqr*esh*
srfshqkekrfsprnprs*qiprktlnnlsrtxvfk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI269 (VHI269Q) /CSM/VH/VHI2-C/VHI269Q.Seq.d/                       2367   0.0
VHQ491 (VHQ491Q) /CSM/VH/VHQ4-D/VHQ491Q.Seq.d/                       1257   0.0
VHQ483 (VHQ483Q) /CSM/VH/VHQ4-D/VHQ483Q.Seq.d/                       1257   0.0
VHQ476 (VHQ476Q) /CSM/VH/VHQ4-D/VHQ476Q.Seq.d/                       1257   0.0
VHQ473 (VHQ473Q) /CSM/VH/VHQ4-D/VHQ473Q.Seq.d/                       1257   0.0
VHQ453 (VHQ453Q) /CSM/VH/VHQ4-C/VHQ453Q.Seq.d/                       1257   0.0
VHQ434 (VHQ434Q) /CSM/VH/VHQ4-B/VHQ434Q.Seq.d/                       1257   0.0
VHQ410 (VHQ410Q) /CSM/VH/VHQ4-A/VHQ410Q.Seq.d/                       1257   0.0
VHQ359 (VHQ359Q) /CSM/VH/VHQ3-C/VHQ359Q.Seq.d/                       1257   0.0
VHQ351 (VHQ351Q) /CSM/VH/VHQ3-C/VHQ351Q.Seq.d/                       1257   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ441715) Dictyostelium discoideum cDNA clone:ddv47i18, 3' ...  1265   0.0    1
(BJ446824) Dictyostelium discoideum cDNA clone:ddv63o22, 3' ...  1257   0.0    1
(BJ446821) Dictyostelium discoideum cDNA clone:ddv63o19, 3' ...  1257   0.0    1
(BJ446813) Dictyostelium discoideum cDNA clone:ddv63m22, 3' ...  1257   0.0    1
(BJ446806) Dictyostelium discoideum cDNA clone:ddv63k22, 3' ...  1257   0.0    1
(BJ446805) Dictyostelium discoideum cDNA clone:ddv63k21, 3' ...  1257   0.0    1
(BJ446797) Dictyostelium discoideum cDNA clone:ddv63i24, 3' ...  1257   0.0    1
(BJ446787) Dictyostelium discoideum cDNA clone:ddv63h20, 3' ...  1257   0.0    1
(BJ446785) Dictyostelium discoideum cDNA clone:ddv63g24, 3' ...  1257   0.0    1
(BJ446779) Dictyostelium discoideum cDNA clone:ddv63f24, 3' ...  1257   0.0    1
dna update 2008.11.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   269   1e-70
DQ848932_1(DQ848932|pid:none) Scophthalmus maximus clone kbc101 ...   267   5e-70
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   266   9e-70
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   265   2e-69
A40411(A40411) translation elongation factor eEF-2 - Caenorhabdi...   265   3e-69
BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   265   3e-69
BT059038_1(BT059038|pid:none) Salmo salar clone ssal-rgf-521-328...   265   3e-69
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   264   6e-69
BT072304_1(BT072304|pid:none) Salmo salar clone ssal-rgf-520-280...   263   7e-69
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   263   1e-68
protein update 2009. 7.13
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHI269 is cyt
5' end seq. ID VHI269F
5' end seq. 
>VHI269F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATCTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAGTCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTNNNN
NNNNNN
Length of 5' end seq. 606
3' end seq. ID VHI269Z
3' end seq. 
>VHI269Z.Seq
NNNNNNNNNNCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCA
ATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGG
TGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCA
CACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTA
TGCTGCTGAATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTAC
TGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGT
TATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTTATTTACCAGT
ATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCC
ACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCAC
TGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGA
CAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTNAGTTTTCAAA
Length of 3' end seq. 651
Connected seq. ID VHI269P
Connected seq. 
>VHI269P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATCTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAGTCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGT----
------CCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATAT
TTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTT
GTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACT
GATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCT
GCTGAATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCC
CCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATT
GGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTTATTTACCAGTATTA
GAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAA
TGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAA
GTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAA
TTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTNAGTTTTCAAA
Length of connected seq. 1237
Full length Seq ID -
Full length Seq. -
Length of full length seq. -