NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI268
Library VH
(Link to library)
Clone ID VHI268
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16305-1
Original site URL
Representative seq. ID VHI268P
(Link to Original site)
Representative DNA sequence 
>VHI268 (VHI268Q) /CSM/VH/VHI2-C/VHI268Q.Seq.d/
ATTGATAAATAAATACAAAAAAAATGAACAGATTATTTAAAGTTCAATCTGTAATTTCAA
AACCAGTTGGATGGTCAAGCATTAGAAGTTTTCACTCATCTAGTGCAAACTATATGTTTG
AAAAGATTTTAATTGCAAATAGAGGTGAAATTGCATGTAGAGTTATGGAAACATGTAAAA
AGATGGGTATTAAAACAGTAGCAATTCATAGTGATGTTGATAAGAGTGCTAAACATGTAA
ATATGGCAGATGAAGCAATTTGTGTTGGACCAGCACCAACATCAGAATCATATTTGAATA
TTGATGCAATTGTAGAGGCAATTAAGATGACAGGTGCACAAGCAGTACATCCAGGTTATG
GATTTTTATCAGAGAATTCAAGATTTGTAAAAGAGTTGGAGAATATTGGTGTAACATTCA
TTGGACCAGGATCATATGCTATGCATGCATTAGGAGATAAGATCGAATCAAAACGTATTG
CCAAAGAGGCAGGTGTTAGTGTTGTACCAGGTTACATTGGAGAAGTCGATGGTATGGATC
ATGTTATAAAGATTGCCAACXXXXXXXXXXTCGTCGATGGTAATGTCACTACCGATTTCA
TTCCAAAGGAGTATCCAGAAGGTTTCCAAGGTTATAAATTCTCTCCAAAACAAATCGAAC
AACTCTCAATTTGTGCAGTTTTCTTACGTTTTGTTGAACAACACAAAAATTTGACAAACT
CTTCAAATACAAATCGTACCTCTTCCATTTCACCAGATATTCTTTACATTGCCAAGGTTG
AAAATGTTCGTCACTCCATTCATTTCTATCCACTCGATAATTCTGAAACTGACGCCGCTG
CCAATGGTGCCAATGGTGATGATTCCTCAAGATTCATGGTTCAATTCATCGATCAAGAGA
AAGATGGTCAAGTCATTAAGGAATTTGAATTAAATTTAGTCGATGCAACTCCACAATTTA
TCAAATTAAACATTGGTGGTATCTCTCATCGTTTCCAAATTCACGATAAAACTTCCACAT
CCTACACCCTTCAATTCCATGGTTCAAAGATTCCAGTCACCGTTTTATCTCCAGAAGAAG
ATTTACTCTGCCAATACATGCCAGTTAAAAAAACTGTTGACTCTTCAAACTCTTTAATCT
CACCAATGCCTGGTACTATCCTCTCTTTGGCCGTTAATGTAGGTGATAAAGTTGTCCTTG
GTCAAGAACTTTGTATCGTTGAAGCAATGAAAATGCAAAATGTTCTCAGAGCTCCAAAAG
ATTGGAAATTAAATCAATCAATGTTAAACCGGTCAAGTCGTTTCTGTGNANAAGTTTNA
sequence update 2002.10.25
Translated Amino Acid sequence 
**INTKKMNRLFKVQSVISKPVGWSSIRSFHSSSANYMFEKILIANRGEIACRVMETCKK
MGIKTVAIHSDVDKSAKHVNMADEAICVGPAPTSESYLNIDAIVEAIKMTGAQAVHPGYG
FLSENSRFVKELENIGVTFIGPGSYAMHALGDKIESKRIAKEAGVSVVPGYIGEVDGMDH
VIKIAN---

---VDGNVTTDFIPKEYPEGFQGYKFSPKQIEQLSICAVFLRFVEQHKNLTNSSNTNRTS
SISPDILYIAKVENVRHSIHFYPLDNSETDAAANGANGDDSSRFMVQFIDQEKDGQVIKE
FELNLVDATPQFIKLNIGGISHRFQIHDKTSTSYTLQFHGSKIPVTVLSPEEDLLCQYMP
VKKTVDSSNSLISPMPGTILSLAVNVGDKVVLGQELCIVEAMKMQNVLRAPKDWKLNQSM
LNRSSRFCXXVX
Translated Amino Acid sequence (All Frames) 
Frame A:
idk*iqkk*tdylkfnl*fqnqldgqalevfthlvqticlkrf*lqievklhvelwkhvk
rwvlkq*qfivmlirvlnm*iwqmkqfvldqhqhqnhi*ilmql*rqlr*qvhkqyiqvm
dfyqriqdl*kswrilv*hsldqdhmlcmh*eirsnqnvlpkrqvlvlyqvtleksmvwi
ml*rlp---

---ssmvmslpisfqrsiqkvskvinslqnksnnsqfvqfsyvllnntki*qtlqiqivp
lpfhqifftlprlkmfvtpfisihsiilkltplpmvpmvmipqdswfnssikrkmvkslr
nln*i*smqlhnlsn*tlvvslivskftiklphptpfnsmvqrfqspfylqkkiysantc
qlkklltlqtl*shqclvlsslwplm*viklslvknfvslkq*kckmfselqkign*inq
c*tgqvvsvxkf

Frame B:
linkykkneqii*ssicnfktswmvkh*kfsli*cklyv*kdfnck*r*ncm*sygnm*k
dgy*nssns**c**ec*tckygr*snlcwtstniriifey*cncrgn*ddrctsstsrlw
ifirefkickrvgeywcnihwtriicyacirr*driktycqrgrc*cctrlhwrsrwygs
cykdcq---

---rrw*chyrfhskgvsrrfprl*ilsktnrttlnlcsfltfc*ttqkfdklfkyksyl
fhftryslhcqg*kcsslhsflstr*f*n*rrcqwcqw**flkihgsihrsrerwssh*g
i*ikfsrcnstiyqikhwwylssfpnsr*nfhilhpsipwfkdsshrfisrrrftlpiha
s*knc*lfklfnltnawyyplfgr*cr**scpwsrtlyr*snenakcsqsskrleiksin
vkpvksflxxsx

Frame C:
**INTKKMNRLFKVQSVISKPVGWSSIRSFHSSSANYMFEKILIANRGEIACRVMETCKK
MGIKTVAIHSDVDKSAKHVNMADEAICVGPAPTSESYLNIDAIVEAIKMTGAQAVHPGYG
FLSENSRFVKELENIGVTFIGPGSYAMHALGDKIESKRIAKEAGVSVVPGYIGEVDGMDH
VIKIAN---

---VDGNVTTDFIPKEYPEGFQGYKFSPKQIEQLSICAVFLRFVEQHKNLTNSSNTNRTS
SISPDILYIAKVENVRHSIHFYPLDNSETDAAANGANGDDSSRFMVQFIDQEKDGQVIKE
FELNLVDATPQFIKLNIGGISHRFQIHDKTSTSYTLQFHGSKIPVTVLSPEEDLLCQYMP
VKKTVDSSNSLISPMPGTILSLAVNVGDKVVLGQELCIVEAMKMQNVLRAPKDWKLNQSM
LNRSSRFCXXVX
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI268 (VHI268Q) /CSM/VH/VHI2-C/VHI268Q.Seq.d/                       2450   0.0
VHJ518 (VHJ518Q) /CSM/VH/VHJ5-A/VHJ518Q.Seq.d/                       1402   0.0
VHP471 (VHP471Q) /CSM/VH/VHP4-C/VHP471Q.Seq.d/                       1400   0.0
AHN143 (AHN143Q) /CSM/AH/AHN1-B/AHN143Q.Seq.d/                       1392   0.0
VHO520 (VHO520Q) /CSM/VH/VHO5-A/VHO520Q.Seq.d/                       1390   0.0
VHM613 (VHM613Q) /CSM/VH/VHM6-A/VHM613Q.Seq.d/                       1298   0.0
AFK260 (AFK260Q) /CSM/AF/AFK2-C/AFK260Q.Seq.d/                       1291   0.0
VHC816 (VHC816Q) /CSM/VH/VHC8-A/VHC816Q.Seq.d/                       1257   0.0
VFC516 (VFC516Q) /CSM/VF/VFC5-A/VFC516Q.Seq.d/                       1245   0.0
VHN793 (VHN793Q) /CSM/VH/VHN7-D/VHN793Q.Seq.d/                       1241   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ441710) Dictyostelium discoideum cDNA clone:ddv47g18, 3' ...  1063   0.0    2
(BJ422955) Dictyostelium discoideum cDNA clone:ddv47g18, 5' ...  1063   0.0    2
(BJ446112) Dictyostelium discoideum cDNA clone:ddv61n18, 3' ...  1057   0.0    3
(BJ427781) Dictyostelium discoideum cDNA clone:ddv63h13, 5' ...  1055   0.0    1
(BJ426738) Dictyostelium discoideum cDNA clone:ddv60g05, 5' ...  1055   0.0    2
(BJ425269) Dictyostelium discoideum cDNA clone:ddv55n04, 5' ...  1055   0.0    1
(BJ424589) Dictyostelium discoideum cDNA clone:ddv52n20, 5' ...  1055   0.0    2
(BJ424576) Dictyostelium discoideum cDNA clone:ddv52l19, 5' ...  1055   0.0    2
(BJ421240) Dictyostelium discoideum cDNA clone:ddv41n01, 5' ...  1055   0.0    2
(BJ419495) Dictyostelium discoideum cDNA clone:ddv36d12, 5' ...  1055   0.0    2
dna update 2008.11.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BX088652_4(BX088652|pid:none) Zebrafish DNA sequence from clone ...   214   4e-54
BC006915_1(BC006915|pid:none) Mus musculus propionyl-Coenzyme A ...   210   1e-52
AY046947_1(AY046947|pid:none) Mus musculus propionyl CoA-carboxy...   210   1e-52
(Q91ZA3) RecName: Full=Propionyl-CoA carboxylase alpha chain, mi...   210   1e-52
M22631_1(M22631|pid:none) Rat alpha-propionyl-CoA carboxylase mR...   208   4e-52
A34337(A34337;A30871)propionyl-CoA carboxylase (EC 6.4.1.3) alph...   208   4e-52
AB171076_1(AB171076|pid:none) Macaca fascicularis brain cDNA clo...   203   1e-50
(P05165) RecName: Full=Propionyl-CoA carboxylase alpha chain, mi...   203   1e-50
AF385926_1(AF385926|pid:none) Homo sapiens propionyl-CoA carboxy...   203   1e-50
FN357591_3(FN357591|pid:none) Schistosoma mansoni genome sequenc...   202   2e-50
protein update 2009. 7.13
PSORT 

 psg: 0.72  gvh: 0.47  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.49  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 72.0 %: mitochondrial
	 16.0 %: nuclear
	  8.0 %: cytoplasmic
	  4.0 %: cytoskeletal

>> prediction for VHI268 is mit
5' end seq. ID VHI268F
5' end seq. 
>VHI268F.Seq
ATTGATAAATAAATACAAAAAAAATGAACAGATTATTTAAAGTTCAATCTGTAATTTCAA
AACCAGTTGGATGGTCAAGCATTAGAAGTTTTCACTCATCTAGTGCAAACTATATGTTTG
AAAAGATTTTAATTGCAAATAGAGGTGAAATTGCATGTAGAGTTATGGAAACATGTAAAA
AGATGGGTATTAAAACAGTAGCAATTCATAGTGATGTTGATAAGAGTGCTAAACATGTAA
ATATGGCAGATGAAGCAATTTGTGTTGGACCAGCACCAACATCAGAATCATATTTGAATA
TTGATGCAATTGTAGAGGCAATTAAGATGACAGGTGCACAAGCAGTACATCCAGGTTATG
GATTTTTATCAGAGAATTCAAGATTTGTAAAAGAGTTGGAGAATATTGGTGTAACATTCA
TTGGACCAGGATCATATGCTATGCATGCATTAGGAGATAAGATCGAATCAAAACGTATTG
CCAAAGAGGCAGGTGTTAGTGTTGTACCAGGTTACATTGGAGAAGTCGATGGTATGGATC
ATGTTATAAAGATTGCCAACNNNNNNNNNN
Length of 5' end seq. 570
3' end seq. ID VHI268Z
3' end seq. 
>VHI268Z.Seq
NNNNNNNNNNTCGTCGATGGTAATGTCACTACCGATTTCATTCCAAAGGAGTATCCAGAA
GGTTTCCAAGGTTATAAATTCTCTCCAAAACAAATCGAACAACTCTCAATTTGTGCAGTT
TTCTTACGTTTTGTTGAACAACACAAAAATTTGACAAACTCTTCAAATACAAATCGTACC
TCTTCCATTTCACCAGATATTCTTTACATTGCCAAGGTTGAAAATGTTCGTCACTCCATT
CATTTCTATCCACTCGATAATTCTGAAACTGACGCCGCTGCCAATGGTGCCAATGGTGAT
GATTCCTCAAGATTCATGGTTCAATTCATCGATCAAGAGAAAGATGGTCAAGTCATTAAG
GAATTTGAATTAAATTTAGTCGATGCAACTCCACAATTTATCAAATTAAACATTGGTGGT
ATCTCTCATCGTTTCCAAATTCACGATAAAACTTCCACATCCTACACCCTTCAATTCCAT
GGTTCAAAGATTCCAGTCACCGTTTTATCTCCAGAAGAAGATTTACTCTGCCAATACATG
CCAGTTAAAAAAACTGTTGACTCTTCAAACTCTTTAATCTCACCAATGCCTGGTACTATC
CTCTCTTTGGCCGTTAATGTAGGTGATAAAGTTGTCCTTGGTCAAGAACTTTGTATCGTT
GAAGCAATGAAAATGCAAAATGTTCTCAGAGCTCCAAAAGATTGGAAATTAAATCAATCA
ATGTTAAACCGGTCAAGTCGTTTCTGTGNANAAGTTTNA
Length of 3' end seq. 759
Connected seq. ID VHI268P
Connected seq. 
>VHI268P.Seq
ATTGATAAATAAATACAAAAAAAATGAACAGATTATTTAAAGTTCAATCTGTAATTTCAA
AACCAGTTGGATGGTCAAGCATTAGAAGTTTTCACTCATCTAGTGCAAACTATATGTTTG
AAAAGATTTTAATTGCAAATAGAGGTGAAATTGCATGTAGAGTTATGGAAACATGTAAAA
AGATGGGTATTAAAACAGTAGCAATTCATAGTGATGTTGATAAGAGTGCTAAACATGTAA
ATATGGCAGATGAAGCAATTTGTGTTGGACCAGCACCAACATCAGAATCATATTTGAATA
TTGATGCAATTGTAGAGGCAATTAAGATGACAGGTGCACAAGCAGTACATCCAGGTTATG
GATTTTTATCAGAGAATTCAAGATTTGTAAAAGAGTTGGAGAATATTGGTGTAACATTCA
TTGGACCAGGATCATATGCTATGCATGCATTAGGAGATAAGATCGAATCAAAACGTATTG
CCAAAGAGGCAGGTGTTAGTGTTGTACCAGGTTACATTGGAGAAGTCGATGGTATGGATC
ATGTTATAAAGATTGCCAAC----------TCGTCGATGGTAATGTCACTACCGATTTCA
TTCCAAAGGAGTATCCAGAAGGTTTCCAAGGTTATAAATTCTCTCCAAAACAAATCGAAC
AACTCTCAATTTGTGCAGTTTTCTTACGTTTTGTTGAACAACACAAAAATTTGACAAACT
CTTCAAATACAAATCGTACCTCTTCCATTTCACCAGATATTCTTTACATTGCCAAGGTTG
AAAATGTTCGTCACTCCATTCATTTCTATCCACTCGATAATTCTGAAACTGACGCCGCTG
CCAATGGTGCCAATGGTGATGATTCCTCAAGATTCATGGTTCAATTCATCGATCAAGAGA
AAGATGGTCAAGTCATTAAGGAATTTGAATTAAATTTAGTCGATGCAACTCCACAATTTA
TCAAATTAAACATTGGTGGTATCTCTCATCGTTTCCAAATTCACGATAAAACTTCCACAT
CCTACACCCTTCAATTCCATGGTTCAAAGATTCCAGTCACCGTTTTATCTCCAGAAGAAG
ATTTACTCTGCCAATACATGCCAGTTAAAAAAACTGTTGACTCTTCAAACTCTTTAATCT
CACCAATGCCTGGTACTATCCTCTCTTTGGCCGTTAATGTAGGTGATAAAGTTGTCCTTG
GTCAAGAACTTTGTATCGTTGAAGCAATGAAAATGCAAAATGTTCTCAGAGCTCCAAAAG
ATTGGAAATTAAATCAATCAATGTTAAACCGGTCAAGTCGTTTCTGTGNANAAGTTTNA
Length of connected seq. 1309
Full length Seq ID -
Full length Seq. -
Length of full length seq. -