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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI263
Library VH
(Link to library)
Clone ID VHI263
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16423-1
Original site URL
Representative seq. ID VHI263P
(Link to Original site)
Representative DNA sequence 
>VHI263 (VHI263Q) /CSM/VH/VHI2-C/VHI263Q.Seq.d/
GGAATTTTCATCCACGCGGATAAATTTTTTTAAAGAATTAGTCATTTTATGCCAATTGGA
AATAACAAAGATAGCGATGGAACTACCCTGAATATCTCAGGGTTAACCATCTCTGATAAG
AGTTCGTCAACTTCATCAAACAACAACACAACAACAACAACAACACAAACTAGTGAACCA
TATGTCCCACCATCTCGTAGAAACAAGATGAATAATAGTTCAGGTTCATCAAGAGACGAT
TATCCATCACCATCTGACCGTGATAATCGTGATTCACCATTCAATCGTGACCGTCGTGAC
GATGGTTACAGAGGTGGTAACAATTTTAGGGAAAATAACTCTAATAACAACAACAACAGT
AGAGATAACTACAAAAGTCCATCATTCTCAAGAAACAATAATAGTAATGGTGGTGGTAGT
AGTAGTGGTAGCGGTGGTTGGGATAATGGTCCAAGAGAACAAGGCCCACCAAGAGGTAAT
AGTTACTCTTATTCACCAAATAATTCATTCTCTCGTGGTGGTAATCAAGGTGGTTATGGT
AATAGAGGCGGAAGTGGCAGTAGTTTTGGTAXXXXXXXXXXTTGGTGTTGTTGGTACCAC
TCAAAATATCACACAAAGAATTGAATACGTAGTTGAAGAGGATAAAAACAGTTACTTGTT
AGATTATCTTAGTGGTTTGAAATCTGATGGTCTTTGTCTCATTTTCGTTGAAACTAAACG
TTCTTGTGATACTCTTACCTACTTTTTAAATCAAAGAAATTTCCCAACTACCTGTATTCA
TGGTGATTTAACTCAACCAGAAAGAGAAAATGCACTTCAATCTTTCCGTTCTTTTGCAAC
TCCATTCTTAGTAGCAACTGATATTGCATCAAGAGGTCTCCACATCGGTAATGTTAATTT
GGTTATCAATTTCGACTTGCCTACCGACATACATATCTACGTTCATCGTATTGGTAGAAC
CGGAAGAGCAGGAAAAAAAGGTTTAGCAATTTCCTTCTTCAATGAAAAGAATAAACCAGT
TGGTGCTGAACTTTTGAAATTAATGAAAGCTTCAAATCAAGATATTCCAGATTGGTTTGA
AAAGATGGTACACAATCTCCGTATGTCTAAAGGTCCATCAAACTCAAAATCAAATAGTCC
ATTCAACAAATCTTACAATAGTCATCACAATAGAGATGATAACCGTGGTGGTTATGGTGG
TGGTGGTGGTGGTAATGGAGGTTACAGTAACAACCGTAATGATAGAAGAGAAGATAAATC
AGATTACTCAATGCATCACCCATACTTTAGCAACAATGGTGGTTCATATAATTAAACATT
CACCTCATGTCATTCGTTTAATAAAAAATAATAAATCCATTTNATTCAATA
sequence update 2002.10.25
Translated Amino Acid sequence 
gifihadkff*RISHFMPIGNNKDSDGTTLNISGLTISDKSSSTSSNNNTTTTTTQTSEP
YVPPSRRNKMNNSSGSSRDDYPSPSDRDNRDSPFNRDRRDDGYRGGNNFRENNSNNNNNS
RDNYKSPSFSRNNNSNGGGSSSGSGGWDNGPREQGPPRGNSYSYSPNNSFSRGGNQGGYG
NRGGSGSSFG---

---GVVGTTQNITQRIEYVVEEDKNSYLLDYLSGLKSDGLCLIFVETKRSCDTLTYFLNQ
RNFPTTCIHGDLTQPERENALQSFRSFATPFLVATDIASRGLHIGNVNLVINFDLPTDIH
IYVHRIGRTGRAGKKGLAISFFNEKNKPVGAELLKLMKASNQDIPDWFEKMVHNLRMSKG
PSNSKSNSPFNKSYNSHHNRDDNRGGYGGGGGGNGGYSNNRNDRREDKSDYSMHHPYFSN
NGGSYN*tftschsfnkk**ihxiq
Translated Amino Acid sequence (All Frames) 
Frame A:
gifihadkff*RISHFMPIGNNKDSDGTTLNISGLTISDKSSSTSSNNNTTTTTTQTSEP
YVPPSRRNKMNNSSGSSRDDYPSPSDRDNRDSPFNRDRRDDGYRGGNNFRENNSNNNNNS
RDNYKSPSFSRNNNSNGGGSSSGSGGWDNGPREQGPPRGNSYSYSPNNSFSRGGNQGGYG
NRGGSGSSFG---

---lvllvplkishkelnt*lkriktvtc*iilvv*nlmvfvsfslklnvlvillptf*i
keisqlpvfmvi*lnqkekmhfnlsvllqlhs**qlilhqevstsvmliwlsistclpty
istfivlvepeeqekkv*qfpssmkrinqlvlnf*n**klqikifqiglkrwytisvclk
vhqtqnqivhstnltivitiemitvvvmvvvvvvmevtvttvmieekinqitqcithtla
tmvvhiikhsphvirliknnksixfn

Frame B:
efsstrinffkelvilcqleitkiamelp*isqg*pslirvrqlhqtttqqqqqhklvnh
mshhlvetr*iivqvhqetiihhhltviivihhsivtvvtmvtevvtilgkitlittttv
eittkvhhsqetiivmvvvvvvvavvgimvqenkahqevivtlihqiihslvvvikvvmv
ieaevavvlv---

---wccwyhskyhtkn*irs*rg*kqllvrls*wfei*wslshfr*n*tfl*ysyllfks
kkfpnylysw*fnstrkrkctsifpffcnsilssn*ycikrsphr*c*fgyqfrlayrht
ylrssyw*nrksrkkrfsnfllq*ke*tswc*tfeinesfksrysrlv*kdgtqspyv*r
siklkik*siqqilq*ssq*r**pwwlwwwwww*wrlq*qp***krr*irllnaspil*q
qwwfi*lnihlmsfv**kiinpfxsi

Frame C:
nfhprg*iflkn*sfyanwk*qr*rwnypeylrvnhl**efvnfikqqhnnnnntn**ti
cptis*kqde**frfikrrlsiti*p**s*ftiqs*ps*rwlqrw*qf*gk*l**qqqq*
r*lqksiilkkq***www***w*rwlg*wskrtrptkr**lllftk*filsww*srwlw*
*rrkwq*fw---

---GVVGTTQNITQRIEYVVEEDKNSYLLDYLSGLKSDGLCLIFVETKRSCDTLTYFLNQ
RNFPTTCIHGDLTQPERENALQSFRSFATPFLVATDIASRGLHIGNVNLVINFDLPTDIH
IYVHRIGRTGRAGKKGLAISFFNEKNKPVGAELLKLMKASNQDIPDWFEKMVHNLRMSKG
PSNSKSNSPFNKSYNSHHNRDDNRGGYGGGGGGNGGYSNNRNDRREDKSDYSMHHPYFSN
NGGSYN*tftschsfnkk**ihxiq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI263 (VHI263Q) /CSM/VH/VHI2-C/VHI263Q.Seq.d/                       1909   0.0
AFC476 (AFC476Q) /CSM/AF/AFC4-D/AFC476Q.Seq.d/                       1154   0.0
CHF591 (CHF591Q) /CSM/CH/CHF5-D/CHF591Q.Seq.d/                       1152   0.0
CHH489 (CHH489Q) /CSM/CH/CHH4-D/CHH489Q.Seq.d/                       1094   0.0
CHQ411 (CHQ411Q) /CSM/CH/CHQ4-A/CHQ411Q.Seq.d/                       1078   0.0
VHP121 (VHP121Q) /CSM/VH/VHP1-A/VHP121Q.Seq.d/                       1066   0.0
CHQ257 (CHQ257Q) /CSM/CH/CHQ2-C/CHQ257Q.Seq.d/                       1039   0.0
CHL756 (CHL756Q) /CSM/CH/CHL7-C/CHL756Q.Seq.d/                        952   0.0
AFM278 (AFM278Q) /CSM/AF/AFM2-D/AFM278Q.Seq.d/                        946   0.0
VHQ404 (VHQ404Q) /CSM/VH/VHQ4-A/VHQ404Q.Seq.d/                        934   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ441733) Dictyostelium discoideum cDNA clone:ddv47m16, 3' ...   775   0.0    3
(BJ379108) Dictyostelium discoideum cDNA clone:ddc33e23, 3' ...   773   0.0    3
(BJ358087) Dictyostelium discoideum cDNA clone:dda65p14, 3' ...   726   0.0    5
(BJ380023) Dictyostelium discoideum cDNA clone:ddc36b24, 3' ...   716   0.0    3
(BJ385167) Dictyostelium discoideum cDNA clone:ddc54f04, 3' ...   706   0.0    3
(BJ445934) Dictyostelium discoideum cDNA clone:ddv61i05, 3' ...   700   0.0    3
(BJ385297) Dictyostelium discoideum cDNA clone:ddc54a16, 3' ...   672   0.0    3
(BJ382734) Dictyostelium discoideum cDNA clone:ddc45p13, 3' ...   573   0.0    3
(BJ347026) Dictyostelium discoideum cDNA clone:dda25k20, 3' ...   565   0.0    3
(BJ446556) Dictyostelium discoideum cDNA clone:ddv63h02, 3' ...   555   0.0    3
dna update 2008.11.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54QS3) RecName: Full=Probable ATP-dependent RNA helicase ddx3;...   390   e-107
(Q6Z4K6) RecName: Full=DEAD-box ATP-dependent RNA helicase 52B; ...   186   1e-45
AM048814_1(AM048814|pid:none) Platynereis dumerilii mRNA for PL1...   185   4e-45
(Q75HJ0) RecName: Full=DEAD-box ATP-dependent RNA helicase 37;  ...   184   5e-45
(O13370) RecName: Full=ATP-dependent RNA helicase ded1;         ...   184   5e-45
BT085991_1(BT085991|pid:none) Zea mays full-length cDNA clone ZM...   183   1e-44
EU953399_1(EU953399|pid:none) Zea mays clone 1406878 unknown mRNA.    182   2e-44
(A1CXK7) RecName: Full=ATP-dependent RNA helicase ded1;         ...   181   4e-44
CU640366_940(CU640366|pid:none) Podospora anserina genomic DNA c...   181   5e-44
(A1CH78) RecName: Full=ATP-dependent RNA helicase ded1;         ...   181   5e-44
protein update 2009. 7.13
PSORT 

 psg: 0.88  gvh: 0.39  alm: 0.43  top: 0.30  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.11  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 44.0 %: nuclear
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VHI263 is cyt
5' end seq. ID VHI263F
5' end seq. 
>VHI263F.Seq
GGAATTTTCATCCACGCGGATAAATTTTTTTAAAGAATTAGTCATTTTATGCCAATTGGA
AATAACAAAGATAGCGATGGAACTACCCTGAATATCTCAGGGTTAACCATCTCTGATAAG
AGTTCGTCAACTTCATCAAACAACAACACAACAACAACAACAACACAAACTAGTGAACCA
TATGTCCCACCATCTCGTAGAAACAAGATGAATAATAGTTCAGGTTCATCAAGAGACGAT
TATCCATCACCATCTGACCGTGATAATCGTGATTCACCATTCAATCGTGACCGTCGTGAC
GATGGTTACAGAGGTGGTAACAATTTTAGGGAAAATAACTCTAATAACAACAACAACAGT
AGAGATAACTACAAAAGTCCATCATTCTCAAGAAACAATAATAGTAATGGTGGTGGTAGT
AGTAGTGGTAGCGGTGGTTGGGATAATGGTCCAAGAGAACAAGGCCCACCAAGAGGTAAT
AGTTACTCTTATTCACCAAATAATTCATTCTCTCGTGGTGGTAATCAAGGTGGTTATGGT
AATAGAGGCGGAAGTGGCAGTAGTTTTGGTANNNNNNNNNN
Length of 5' end seq. 581
3' end seq. ID VHI263Z
3' end seq. 
>VHI263Z.Seq
NNNNNNNNNNTTGGTGTTGTTGGTACCACTCAAAATATCACACAAAGAATTGAATACGTA
GTTGAAGAGGATAAAAACAGTTACTTGTTAGATTATCTTAGTGGTTTGAAATCTGATGGT
CTTTGTCTCATTTTCGTTGAAACTAAACGTTCTTGTGATACTCTTACCTACTTTTTAAAT
CAAAGAAATTTCCCAACTACCTGTATTCATGGTGATTTAACTCAACCAGAAAGAGAAAAT
GCACTTCAATCTTTCCGTTCTTTTGCAACTCCATTCTTAGTAGCAACTGATATTGCATCA
AGAGGTCTCCACATCGGTAATGTTAATTTGGTTATCAATTTCGACTTGCCTACCGACATA
CATATCTACGTTCATCGTATTGGTAGAACCGGAAGAGCAGGAAAAAAAGGTTTAGCAATT
TCCTTCTTCAATGAAAAGAATAAACCAGTTGGTGCTGAACTTTTGAAATTAATGAAAGCT
TCAAATCAAGATATTCCAGATTGGTTTGAAAAGATGGTACACAATCTCCGTATGTCTAAA
GGTCCATCAAACTCAAAATCAAATAGTCCATTCAACAAATCTTACAATAGTCATCACAAT
AGAGATGATAACCGTGGTGGTTATGGTGGTGGTGGTGGTGGTAATGGAGGTTACAGTAAC
AACCGTAATGATAGAAGAGAAGATAAATCAGATTACTCAATGCATCACCCATACTTTAGC
AACAATGGTGGTTCATATAATTAAACATTCACCTCATGTCATTCGTTTAATAAAAAATAA
TAAATCCATTTNATTCAATA
Length of 3' end seq. 800
Connected seq. ID VHI263P
Connected seq. 
>VHI263P.Seq
GGAATTTTCATCCACGCGGATAAATTTTTTTAAAGAATTAGTCATTTTATGCCAATTGGA
AATAACAAAGATAGCGATGGAACTACCCTGAATATCTCAGGGTTAACCATCTCTGATAAG
AGTTCGTCAACTTCATCAAACAACAACACAACAACAACAACAACACAAACTAGTGAACCA
TATGTCCCACCATCTCGTAGAAACAAGATGAATAATAGTTCAGGTTCATCAAGAGACGAT
TATCCATCACCATCTGACCGTGATAATCGTGATTCACCATTCAATCGTGACCGTCGTGAC
GATGGTTACAGAGGTGGTAACAATTTTAGGGAAAATAACTCTAATAACAACAACAACAGT
AGAGATAACTACAAAAGTCCATCATTCTCAAGAAACAATAATAGTAATGGTGGTGGTAGT
AGTAGTGGTAGCGGTGGTTGGGATAATGGTCCAAGAGAACAAGGCCCACCAAGAGGTAAT
AGTTACTCTTATTCACCAAATAATTCATTCTCTCGTGGTGGTAATCAAGGTGGTTATGGT
AATAGAGGCGGAAGTGGCAGTAGTTTTGGTA----------TTGGTGTTGTTGGTACCAC
TCAAAATATCACACAAAGAATTGAATACGTAGTTGAAGAGGATAAAAACAGTTACTTGTT
AGATTATCTTAGTGGTTTGAAATCTGATGGTCTTTGTCTCATTTTCGTTGAAACTAAACG
TTCTTGTGATACTCTTACCTACTTTTTAAATCAAAGAAATTTCCCAACTACCTGTATTCA
TGGTGATTTAACTCAACCAGAAAGAGAAAATGCACTTCAATCTTTCCGTTCTTTTGCAAC
TCCATTCTTAGTAGCAACTGATATTGCATCAAGAGGTCTCCACATCGGTAATGTTAATTT
GGTTATCAATTTCGACTTGCCTACCGACATACATATCTACGTTCATCGTATTGGTAGAAC
CGGAAGAGCAGGAAAAAAAGGTTTAGCAATTTCCTTCTTCAATGAAAAGAATAAACCAGT
TGGTGCTGAACTTTTGAAATTAATGAAAGCTTCAAATCAAGATATTCCAGATTGGTTTGA
AAAGATGGTACACAATCTCCGTATGTCTAAAGGTCCATCAAACTCAAAATCAAATAGTCC
ATTCAACAAATCTTACAATAGTCATCACAATAGAGATGATAACCGTGGTGGTTATGGTGG
TGGTGGTGGTGGTAATGGAGGTTACAGTAACAACCGTAATGATAGAAGAGAAGATAAATC
AGATTACTCAATGCATCACCCATACTTTAGCAACAATGGTGGTTCATATAATTAAACATT
CACCTCATGTCATTCGTTTAATAAAAAATAATAAATCCATTTNATTCAATA
Length of connected seq. 1361
Full length Seq ID -
Full length Seq. -
Length of full length seq. -