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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI259
Library VH
(Link to library)
Clone ID VHI259
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11746-1
Original site URL
Representative seq. ID VHI259P
(Link to Original site)
Representative DNA sequence 
>VHI259 (VHI259Q) /CSM/VH/VHI2-C/VHI259Q.Seq.d/
AAACAACGCGTAACTATAAAATGCCTTCAGTACAAAAAACCGTCGTAAAGAAATCACATA
AATTCGTCATTGATTGNACCGCCCCAGCCGGTAAAATCGTCGATGTTGCTGCTTTCGAAA
AGTACTTACACGATAGAATCAAAGTTGACAACAAAGTTGGTAACCTCGGTTCAACTGTTG
TTATCTCAAAAGATAAATCAAAGATCATCATCAACACCACCATCCCATTCTCAAAGAGAT
ACCTCAAATATTTAACCAAGAAATTCTTAAAATTCAAACAAATCAGAGATTTCTTACGTG
TTGTTGCCACCACCAAAAACACCTACGAACTCAGATACTTCAACATTGGTGACTCTGAAT
CACAAGAATAAACACTTTGGTCCCAACCTTTGATCAAAATGGTAAACGAATAAATCTGGT
TTAACATTTAAAAAAAAAAXXXXXXXXXXTCGTCGCCGGCACTCCATTAGATAAAGCATA
CACCAGCGAGAATATCGAGCTCCTAAAGAAAGGTGTCAGTAATCTTGCCCATCATATTAA
AAACTTAAAGAAATTTGGTGTCGGTGTAGTGGTCGCCATCAATAAATTCCATACCGATTC
CGATGCCGAAGTTAACCTTTTGGTTGAGGGCTTCTTTAT
sequence update 2002.10.25
Translated Amino Acid sequence 
kqrvtikclqykkps*rnhinsslixppqpvkssmlllskstytiesklttklvtsvqll
lsqkinqrssstppshsqrdtsni*prns*nsnkseisyvllpppktptnsdtstlvtln
hknkhfgpnl*skw*TNKSGLTFKKK---

---VAGTPLDKAYTSENIELLKKGVSNLAHHIKNLKKFGVGVVVAINKFHTDSDAEVNLL
VEGFF
Translated Amino Acid sequence (All Frames) 
Frame A:
kqrvtikclqykkps*rnhinsslixppqpvkssmlllskstytiesklttklvtsvqll
lsqkinqrssstppshsqrdtsni*prns*nsnkseisyvllpppktptnsdtstlvtln
hknkhfgpnl*skw*TNKSGLTFKKK---

---sspalh*ikhtparisss*rkvsvilpiilkt*rnlvsv*wspsinsipipmpkltf
wlrasl

Frame B:
nna*l*nafstknrrkeit*irh*lxrpsr*nrrcccfrkvltr*nqs*qqsw*prfncc
ylkr*ikdhhqhhhpilkeipqifnqeilkiqtnqrfltccchhqkhlrtqilqhw*l*i
trintlvptfdqngkrinlv*hlkkk---

---rrrhsir*sihqreyrapkercq*scpsy*klkeiwcrcsgrhq*ipyrfrcrs*pf
g*glly

Frame C:
ttrnykmpsvqktvvkkshkfvidxtapagkivdvaafekylhdrikvdnkvgnlgstvv
iskdkskiiinttipfskrylkyltkkflkfkqirdflrvvattkntyelryfnigdses
qe*tlwsqplikmvne*iwfni*kk---

---VAGTPLDKAYTSENIELLKKGVSNLAHHIKNLKKFGVGVVVAINKFHTDSDAEVNLL
VEGFF
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI259 (VHI259Q) /CSM/VH/VHI2-C/VHI259Q.Seq.d/                        844   0.0
VSJ148 (VSJ148Q) /CSM/VS/VSJ1-B/VSJ148Q.Seq.d/                        842   0.0
VSI831 (VSI831Q) /CSM/VS/VSI8-B/VSI831Q.Seq.d/                        842   0.0
FC-219 (FC-219Q) /CSM/CG/CG-FC/FC-219Q.Seq.d/                         842   0.0
FC-AM01 (FC-AM01Q) /CSM/FC/FC-AM/FC-AM01Q.Seq.d/                      841   0.0
VSF652 (VSF652Q) /CSM/VS/VSF6-C/VSF652Q.Seq.d/                        839   0.0
FC-AT11 (FC-AT11Q) /CSM/FC/FC-AT/FC-AT11Q.Seq.d/                      829   0.0
VSF864 (VSF864Q) /CSM/VS/VSF8-C/VSF864Q.Seq.d/                        827   0.0
FC-BM18 (FC-BM18Q) /CSM/FC/FC-BM/FC-BM18Q.Seq.d/                      811   0.0
VSG473 (VSG473Q) /CSM/VS/VSG4-D/VSG473Q.Seq.d/                        797   0.0
own update 2002.12.31
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ422942) Dictyostelium discoideum cDNA clone:ddv47e16, 5' ...   844   0.0    1
(AU269480) Dictyostelium discoideum vegetative cDNA clone:VS...   842   0.0    1
(AU269300) Dictyostelium discoideum vegetative cDNA clone:VS...   842   0.0    1
(C23665) Dictyostelium discoideum gamete cDNA, clone FC-AM01.     841   0.0    1
(AU265561) Dictyostelium discoideum vegetative cDNA clone:VS...   839   0.0    1
(AU284410) Dictyostelium discoideum gamete cDNA clone:FC-AT1...   779   0.0    2
(AU265915) Dictyostelium discoideum vegetative cDNA clone:VS...   827   0.0    1
(AU284836) Dictyostelium discoideum gamete cDNA clone:FC-BM1...   811   0.0    1
(AU266539) Dictyostelium discoideum vegetative cDNA clone:VS...   797   0.0    1
(AU269481) Dictyostelium discoideum vegetative cDNA clone:VS...   765   0.0    2
dna update 2008.11.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54JE3) RecName: Full=60S ribosomal protein L22 1;                   152   8e-36
(Q54GK6) RecName: Full=60S ribosomal protein L22 2;                   149   9e-35
AF542529_8(AF542529|pid:none) Cryptococcus neoformans var. grubi...    80   4e-14
AF542530_16(AF542530|pid:none) Cryptococcus neoformans var. neof...    80   4e-14
AY710429_17(AY710429|pid:none) Cryptococcus gattii strain E566 M...    79   1e-13
AY710430_6(AY710430|pid:none) Cryptococcus gattii strain WM276 M...    77   3e-13
AM270020_3(AM270020|pid:none) Aspergillus niger contig An02c0250...    77   4e-13
(P50886) RecName: Full=60S ribosomal protein L22;  &(Q28IL6) Rec...    75   1e-12
AP007151_640(AP007151|pid:none) Aspergillus oryzae RIB40 genomic...    74   4e-12
AJ937764_25(AJ937764|pid:none) Uncultured Chloroflexi bacterium ...    74   4e-12
protein update 2009. 7.13
PSORT 

 psg: 0.66  gvh: 0.24  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 32.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: peroxisomal

>> prediction for VHI259 is nuc
5' end seq. ID VHI259F
5' end seq. 
>VHI259F.Seq
AAACAACGCGTAACTATAAAATGCCTTCAGTACAAAAAACCGTCGTAAAGAAATCACATA
AATTCGTCATTGATTGNACCGCCCCAGCCGGTAAAATCGTCGATGTTGCTGCTTTCGAAA
AGTACTTACACGATAGAATCAAAGTTGACAACAAAGTTGGTAACCTCGGTTCAACTGTTG
TTATCTCAAAAGATAAATCAAAGATCATCATCAACACCACCATCCCATTCTCAAAGAGAT
ACCTCAAATATTTAACCAAGAAATTCTTAAAATTCAAACAAATCAGAGATTTCTTACGTG
TTGTTGCCACCACCAAAAACACCTACGAACTCAGATACTTCAACATTGGTGACTCTGAAT
CACAAGAATAAACACTTTGGTCCCAACCTTTGATCAAAATGGTAAACGAATAAATCTGGT
TTAACATTTAAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 449
3' end seq. ID VHI259Z
3' end seq. 
>VHI259Z.Seq
NNNNNNNNNNTCGTCGCCGGCACTCCATTAGATAAAGCATACACCAGCGAGAATATCGAG
CTCCTAAAGAAAGGTGTCAGTAATCTTGCCCATCATATTAAAAACTTAAAGAAATTTGGT
GTCGGTGTAGTGGTCGCCATCAATAAATTCCATACCGATTCCGATGCCGAAGTTAACCTT
TTGGTTGAGGGCTTCTTTAT
Length of 3' end seq. 200
Connected seq. ID VHI259P
Connected seq. 
>VHI259P.Seq
AAACAACGCGTAACTATAAAATGCCTTCAGTACAAAAAACCGTCGTAAAGAAATCACATA
AATTCGTCATTGATTGNACCGCCCCAGCCGGTAAAATCGTCGATGTTGCTGCTTTCGAAA
AGTACTTACACGATAGAATCAAAGTTGACAACAAAGTTGGTAACCTCGGTTCAACTGTTG
TTATCTCAAAAGATAAATCAAAGATCATCATCAACACCACCATCCCATTCTCAAAGAGAT
ACCTCAAATATTTAACCAAGAAATTCTTAAAATTCAAACAAATCAGAGATTTCTTACGTG
TTGTTGCCACCACCAAAAACACCTACGAACTCAGATACTTCAACATTGGTGACTCTGAAT
CACAAGAATAAACACTTTGGTCCCAACCTTTGATCAAAATGGTAAACGAATAAATCTGGT
TTAACATTTAAAAAAAAAA----------TCGTCGCCGGCACTCCATTAGATAAAGCATA
CACCAGCGAGAATATCGAGCTCCTAAAGAAAGGTGTCAGTAATCTTGCCCATCATATTAA
AAACTTAAAGAAATTTGGTGTCGGTGTAGTGGTCGCCATCAATAAATTCCATACCGATTC
CGATGCCGAAGTTAACCTTTTGGTTGAGGGCTTCTTTAT
Length of connected seq. 629
Full length Seq ID -
Full length Seq. -
Length of full length seq. -