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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO225
Library AF
(Link to library)
Clone ID AFO225
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11477-1
Original site URL
Representative seq. ID AFO225P
(Link to Original site)
Representative DNA sequence 
>AFO225 (AFO225Q) /CSM/AF/AFO2-B/AFO225Q.Seq.d/
AAAGAATTCATTACGTGCTCGTTTAGCTTGGGAAGGTACTGATTGGAAATTACCAAATAA
AGAAATTCTCAATACTAAATTTGTATATGTTGTTGATTGTGTCACTGAAATCTTTGTTTG
GGTTGGTAAAGAATCATCATCAATTCAAAGAAAAATGGCAACTAAAGTTGCATTGGTATT
ACAAGCTCAAAAAGATCGTGTAGATTGGACAAAGATAACAAGATTAACCGAATTTGGTGA
AAATAATCTCTTCAAAGAGAAATTTGCAAATTATCCAGGTATGTTGCCAATTTCAACCAC
TCGTCAAGAGATTAAAAGTCATGTTGCAACCTCAAAGGTTGAGCATAAAATTGAAACATT
GGCATCACGTATGACAACTCCATTCATTGATAATGAAGCCTTATTCACAATACCAATTGA
TGAAGGTGGTCGTGTTAAAGTTTGGAAAATTGAAGACTATGAAAAGATTGATCATCCACA
ACATTTATATTCTCAATTCTTTTCATCAGATAGTTATATCGTTCTCTATACCTATATGCA
AAATAATAAGGAAGCTCATGTTATCTATTATTATTTAGGTAGAGATTCTTCAATCAATGA
AAAAGGTACTTCCGCTTATTTAACAGTTGATCTCAATGAATCATTGGTTGGTGCTXXXXX
XXXXXAACAAGTACTTAAAGATTATCTCAAAGAAATCTATACCTATGAAGNAGTTTATTG
GCCGATCCATTACCTGCTGGCGTTGACTCTACTAAATTGGATACTTATCTCAATGATGAA
GATTTTGAAAAAGTTTTCAAAATGACTAGAACTGAATGGTTAAAGATTCCAGCTTGGAAG
AGAGAAGGTATTAAAAAAGAATTATTCTTATTTTAAATAATAAAAATAATCTCTATCTAT
AATATACATTCATATAAATACCTATATACATATATTTAATACATATAAATTTACTATTTA
AAATAAATAAAAAAAATAAAAAAAAAATTAAATA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
KNSLRARLAWEGTDWKLPNKEILNTKFVYVVDCVTEIFVWVGKESSSIQRKMATKVALVL
QAQKDRVDWTKITRLTEFGENNLFKEKFANYPGMLPISTTRQEIKSHVATSKVEHKIETL
ASRMTTPFIDNEALFTIPIDEGGRVKVWKIEDYEKIDHPQHLYSQFFSSDSYIVLYTYMQ
NNKEAHVIYYYLGRDSSINEKGTSAYLTVDLNESLVGA---

---QVLKDYLKEIYTYEXVYWPIHYLLALTLLNWILISMMKILKKFSK*lelng*rfqlg
rekvlkknysyfk**k*slsiiyihintyihifntykfti*nk*kk*kkn*i
Translated Amino Acid sequence (All Frames) 
Frame A:
kefitcsfslgry*leitk*rnsqy*icicc*lch*nlclgw*riiinskkngn*scigi
tsskrscrldkdnkinriw*k*slqreicklsryvanfnhssrd*ksccnlkg*a*n*ni
gitydnsih***slihntn**rwsc*slen*rl*kd*ssttfifsilfir*lyrslylya
k**gsscylllfr*rffnq*kryfrlfns*sq*iigwc---

---nkylkiiskksipmkxfigrsitcwr*ly*igylsq**rf*ksfqnd*n*mvkdssl
eerry*kriililnnknnlyl*ytfi*ipiyiylihinllfkinkknkkkik

Frame B:
KNSLRARLAWEGTDWKLPNKEILNTKFVYVVDCVTEIFVWVGKESSSIQRKMATKVALVL
QAQKDRVDWTKITRLTEFGENNLFKEKFANYPGMLPISTTRQEIKSHVATSKVEHKIETL
ASRMTTPFIDNEALFTIPIDEGGRVKVWKIEDYEKIDHPQHLYSQFFSSDSYIVLYTYMQ
NNKEAHVIYYYLGRDSSINEKGTSAYLTVDLNESLVGA---

---tst*rlsqrnlyl*xslladplpagvdstkldtylndedfekvfkmtrtewlkipaw
kregikkelflf*iikiisiynihsykylytyi*yi*iyylk*ikkikkkln

Frame C:
rihyvlv*lgkvlignyqikkfsilnlymllivslkslfglvknhhqfkekwqlklhwyy
klkkiv*igqr*qd*pnlvkiisskrnlqiiqvccqfqplvkrlkvmlqpqrlsiklkhw
hhv*qlhslimkpysqyqlmkvvvlkfgklktmkrliihniyilnsfhqivisfsipick
iirklmlsiii*veilqsmkkvlpli*qlismnhwlv---

---QVLKDYLKEIYTYEXVYWPIHYLLALTLLNWILISMMKILKKFSK*lelng*rfqlg
rekvlkknysyfk**k*slsiiyihintyihifntykfti*nk*kk*kkn*i
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO225 (AFO225Q) /CSM/AF/AFO2-B/AFO225Q.Seq.d/                       1653   0.0
AFI116 (AFI116Q) /CSM/AF/AFI1-A/AFI116Q.Seq.d/                        743   0.0
AHO612 (AHO612Q) /CSM/AH/AHO6-A/AHO612Q.Seq.d/                        533   e-150
SSL281 (SSL281Q) /CSM/SS/SSL2-D/SSL281Q.Seq.d/                        369   e-101
SSD748 (SSD748Q) /CSM/SS/SSD7-B/SSD748Q.Seq.d/                        369   e-101
SSB880 (SSB880Q) /CSM/SS/SSB8-D/SSB880Q.Seq.d/                        369   e-101
SHK802 (SHK802Q) /CSM/SH/SHK8-A/SHK802Q.Seq.d/                        369   e-101
CHI516 (CHI516Q) /CSM/CH/CHI5-A/CHI516Q.Seq.d/                        369   e-101
AHI567 (AHI567Q) /CSM/AH/AHI5-C/AHI567Q.Seq.d/                        353   3e-96
AHC554 (AHC554Q) /CSM/AH/AHC5-C/AHC554Q.Seq.d/                        351   1e-95
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AJ427856|AJ427856.1 Dictyostelium discoideum ORF encoding villidin.  1298  0.0  3
U78754|U78754.1 Dictyostelium discoideum villin (vilA) mRNA, partial cds.   303  2e-97  2
BZ393782|BZ393782.1 EINAM68TR EI_10_12_KB Entamoeba invadens genomic clone EINAM68, DNA sequence.    48  0.023  2
AY220913|AY220913.1 Dictyostelium discoideum ComD (comD) gene, complete cds.    40  0.11  2
AZ010756|AZ010756.1 RPCI-23-376A4.TV RPCI-23 Mus musculus genomic clone RPCI-23-376A4, genomic survey sequence.    48  0.21  1
AC113225|AC113225.2 Rattus norvegicus clone RP31-276E17 strain Brown Norway, WORKING DRAFT SEQUENCE, 7 ordered pieces.    38  0.28  3
AC109666|AC109666.7 Rattus norvegicus 4 BAC CH230-252C14 (Children's Hospital Oakland Research Institute) complete sequence.    38  0.42  3
AL391423|AL391423.16 Human DNA sequence from clone RP13-43J18 on chromosome 10.    42  0.50  3
AC091614|AC091614.3 Homo sapiens chromosome 1 clone RP4-665J23, complete sequence.    34  0.62  6
AC101756|AC101756.3 Mus musculus clone RP24-512C9, WORKING DRAFT SEQUENCE, 9 unordered pieces.    48  0.69  2
dna update 2003. 9.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AJ427856_1(AJ427856|pid:none) Dictyostelium discoideum ORF encod...   442   e-123
(Q8WQ85) RecName: Full=Villidin;                                      442   e-123
AF118397_1(AF118397|pid:none) Entamoeba histolytica actin-bindin...   117   6e-25
(P36418) RecName: Full=Protovillin; AltName: Full=100 kDa actin-...   102   2e-20
EU334660_1(EU334660|pid:none) Strongylocentrotus purpuratus vill...    98   4e-19
BT055016_1(BT055016|pid:none) Zea mays full-length cDNA clone ZM...    97   1e-18
AF175294_1(AF175294|pid:none) Danio rerio gelsolin mRNA, complet...    96   1e-18
BC125898_1(BC125898|pid:none) Danio rerio scinderin like a, mRNA...    96   2e-18
BC135025_1(BC135025|pid:none) Danio rerio gelsolin b, mRNA (cDNA...    95   4e-18
BC155581_1(BC155581|pid:none) Danio rerio scinderin like a, mRNA...    95   4e-18
protein update 2009. 4.26
PSORT 

 psg: 0.59  gvh: 0.35  alm: 0.31  top: 0.83  tms: 0.07  mit: 0.30  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 1.00  pox: 0.00  px2: 0.00  vac: 0.33  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 1.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 20.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  8.0 %: endoplasmic reticulum
	  8.0 %: plasma membrane
	  8.0 %: vesicles of secretory system
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for AFO225 is cyt
5' end seq. ID AFO225F
5' end seq. 
>AFO225F.Seq
AAAGAATTCATTACGTGCTCGTTTAGCTTGGGAAGGTACTGATTGGAAATTACCAAATAA
AGAAATTCTCAATACTAAATTTGTATATGTTGTTGATTGTGTCACTGAAATCTTTGTTTG
GGTTGGTAAAGAATCATCATCAATTCAAAGAAAAATGGCAACTAAAGTTGCATTGGTATT
ACAAGCTCAAAAAGATCGTGTAGATTGGACAAAGATAACAAGATTAACCGAATTTGGTGA
AAATAATCTCTTCAAAGAGAAATTTGCAAATTATCCAGGTATGTTGCCAATTTCAACCAC
TCGTCAAGAGATTAAAAGTCATGTTGCAACCTCAAAGGTTGAGCATAAAATTGAAACATT
GGCATCACGTATGACAACTCCATTCATTGATAATGAAGCCTTATTCACAATACCAATTGA
TGAAGGTGGTCGTGTTAAAGTTTGGAAAATTGAAGACTATGAAAAGATTGATCATCCACA
ACATTTATATTCTCAATTCTTTTCATCAGATAGTTATATCGTTCTCTATACCTATATGCA
AAATAATAAGGAAGCTCATGTTATCTATTATTATTTAGGTAGAGATTCTTCAATCAATGA
AAAAGGTACTTCCGCTTATTTAACAGTTGATCTCAATGAATCATTGGTTGGTGCT-----
-----
Length of 5' end seq. 655
3' end seq. ID AFO225Z
3' end seq. 
>AFO225Z.Seq
----------AACAAGTACTTAAAGATTATCTCAAAGAAATCTATACCTATGAAGNAGTT
TATTGGCCGATCCATTACCTGCTGGCGTTGACTCTACTAAATTGGATACTTATCTCAATG
ATGAAGATTTTGAAAAAGTTTTCAAAATGACTAGAACTGAATGGTTAAAGATTCCAGCTT
GGAAGAGAGAAGGTATTAAAAAAGAATTATTCTTATTTTAAATAATAAAAATAATCTCTA
TCTATAATATACATTCATATAAATACCTATATACATATATTTAATACATATAAATTTACT
ATTTAAAATAAATAAAAAAAATAAAAAAAAAATTAAATA
Length of 3' end seq. 329
Connected seq. ID AFO225P
Connected seq. 
>AFO225P.Seq
AAAGAATTCATTACGTGCTCGTTTAGCTTGGGAAGGTACTGATTGGAAATTACCAAATAA
AGAAATTCTCAATACTAAATTTGTATATGTTGTTGATTGTGTCACTGAAATCTTTGTTTG
GGTTGGTAAAGAATCATCATCAATTCAAAGAAAAATGGCAACTAAAGTTGCATTGGTATT
ACAAGCTCAAAAAGATCGTGTAGATTGGACAAAGATAACAAGATTAACCGAATTTGGTGA
AAATAATCTCTTCAAAGAGAAATTTGCAAATTATCCAGGTATGTTGCCAATTTCAACCAC
TCGTCAAGAGATTAAAAGTCATGTTGCAACCTCAAAGGTTGAGCATAAAATTGAAACATT
GGCATCACGTATGACAACTCCATTCATTGATAATGAAGCCTTATTCACAATACCAATTGA
TGAAGGTGGTCGTGTTAAAGTTTGGAAAATTGAAGACTATGAAAAGATTGATCATCCACA
ACATTTATATTCTCAATTCTTTTCATCAGATAGTTATATCGTTCTCTATACCTATATGCA
AAATAATAAGGAAGCTCATGTTATCTATTATTATTTAGGTAGAGATTCTTCAATCAATGA
AAAAGGTACTTCCGCTTATTTAACAGTTGATCTCAATGAATCATTGGTTGGTGCT-----
-----AACAAGTACTTAAAGATTATCTCAAAGAAATCTATACCTATGAAGNAGTTTATTG
GCCGATCCATTACCTGCTGGCGTTGACTCTACTAAATTGGATACTTATCTCAATGATGAA
GATTTTGAAAAAGTTTTCAAAATGACTAGAACTGAATGGTTAAAGATTCCAGCTTGGAAG
AGAGAAGGTATTAAAAAAGAATTATTCTTATTTTAAATAATAAAAATAATCTCTATCTAT
AATATACATTCATATAAATACCTATATACATATATTTAATACATATAAATTTACTATTTA
AAATAAATAAAAAAAATAAAAAAAAAATTAAATA
Length of connected seq. 984
Full length Seq ID -
Full length Seq. -
Length of full length seq. -