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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI257
Library VH
(Link to library)
Clone ID VHI257
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16531-1
Original site URL
Representative seq. ID VHI257P
(Link to Original site)
Representative DNA sequence 
>VHI257 (VHI257Q) /CSM/VH/VHI2-C/VHI257Q.Seq.d/
TTGGTGATATGAATTTCACATGTAATGATCAAACTGCAATTCCAAATGGTAATTACATTG
CATCTAATGGTAGTACAATGTCTTATCAAGATCAATATAGAGCTTGTCCATCTGCCGGTG
CTATCGAAGGTCAAATGGTTAATGGTGAATTTTATGTTGCTGGTTCAAACTATATAGATG
CTGCACTTGATTTCAAAAGTGATGACCGTACATTAAATGTCATTATCACTTTTTTATGGT
GGATATTTTTTGTAATTATCAATATGATTGCTTTGGAATTATTCGATTGGACAAGTGGTG
GTATGCCTCATAAAGTCTATAAAAGAGGAAAGGCTCCTAAAATCAACGATGACGAAGAAG
AGAGACAACAAAATGCAATGGTTGAAAATGCTACAAGTAAAATGAAAGATACTCTTAAAA
TGAGAGAAAGTTGTTTCACTTGGAATCATATTCACTATACCGTTCAATTAAATGGCAAAG
ATTTATTATTGTTAAATGATGTTGAAGGTTGGATTAAACCTGGTCAAATGACTGCATTAA
TGGGTTCATCTGGTGCGGGTAAAACAACATTACTCGATGTATTGGCAAAXXXXXXXXXXT
TGGGTATTCTCTACATCTTTATTGCAATTCCTCAATTTTTAATTCAAAAAGCTTACTTTA
AGAAAGATTATGCCTCTAAATTTTATTCTTGGTGTCCATTTGCCATTTCAATTGTCATTG
TTGAATTACCTTTTGTTGCAGTCGCTGGAACAATTTGTTTCTTTTGTTCTTTTTGGACAG
CAGGTATTTATTACAATGGTGAATATGATTTTTATTTTTATATAACTTTCATCCTCTTCC
TTTTCATTTGTGTTTCACTTGGTCAAGTTGTATCTGCCTTTTGTTTCAATGTTATGTTGG
CTCAAACAATTTTACCACTTTTATTAGTAATGTTATTTCTTTTTTGTGGAGTACTCGTTC
CTTATGAACAAATTCCAAACTTTTGGAAATTCGTTTATCATTCAAATCCATGCCGTTATT
TCTTAGAAGGTGTTGTAACATCAGTTTTAAAGAATGTTTTTGTTGATTGTTCAAATGAAG
ATTTAACCAAATTCTCAAACCCAACAAATTTAACCTGTAAAGAATATTTCAAGCCAACCT
ATGGAAATGTTCGTGCTGTTACCAAAGGCGATGAAAGTGAATGTGGTTATTGTGTTTTTA
AATCAGGTGAAGAATATTATAAAACTCTTGGTTGGTCTTATGAAAACCGTTTAAGAAACT
ATGGTATTTTATGGGCTTTCTTTATTTTCAACATAATTAGGTTGTTTCCATTGTTTATTT
AACAAAAAAACCAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
GDMNFTCNDQTAIPNGNYIASNGSTMSYQDQYRACPSAGAIEGQMVNGEFYVAGSNYIDA
ALDFKSDDRTLNVIITFLWWIFFVIINMIALELFDWTSGGMPHKVYKRGKAPKINDDEEE
RQQNAMVENATSKMKDTLKMRESCFTWNHIHYTVQLNGKDLLLLNDVEGWIKPGQMTALM
GSSGAGKTTLLDVLA---

---LGILYIFIAIPQFLIQKAYFKKDYASKFYSWCPFAISIVIVELPFVAVAGTICFFCS
FWTAGIYYNGEYDFYFYITFILFLFICVSLGQVVSAFCFNVMLAQTILPLLLVMLFLFCG
VLVPYEQIPNFWKFVYHSNPCRYFLEGVVTSVLKNVFVDCSNEDLTKFSNPTNLTCKEYF
KPTYGNVRAVTKGDESECGYCVFKSGEEYYKTLGWSYENRLRNYGILWAFFIFNIIRLFP
LFI*qknq
Translated Amino Acid sequence (All Frames) 
Frame A:
lvi*ishvmiklqfqmvitlhlmvvqclikinielvhlpvlskvkwlmvnfmllvqti*m
lhliskvmtvh*mslslfyggyfl*lsi*llwnysigqvvvcliksikeerllkstmtkk
rdnkmqwlkmlqvk*killk*ekvvslgiiftipfn*makiyyc*mmlkvglnlvk*lh*
wvhlvrvkqhysmywq---

---LGILYIFIAIPQFLIQKAYFKKDYASKFYSWCPFAISIVIVELPFVAVAGTICFFCS
FWTAGIYYNGEYDFYFYITFILFLFICVSLGQVVSAFCFNVMLAQTILPLLLVMLFLFCG
VLVPYEQIPNFWKFVYHSNPCRYFLEGVVTSVLKNVFVDCSNEDLTKFSNPTNLTCKEYF
KPTYGNVRAVTKGDESECGYCVFKSGEEYYKTLGWSYENRLRNYGILWAFFIFNIIRLFP
LFI*qknq

Frame B:
w*yefhm**sncnskw*lhci*w*ynvlsrsi*slsicrcyrrsng*w*ilccwfklyrc
ct*fqk**pyikchyhffmvdifcnyqydcfgiirldkwwyas*sl*krkgs*nqr*rrr
ettkcng*kcyk*nerys*nerklfhlesyslyrsikwqrfiivk*c*rld*twsndcin
gfiwcg*nnitrcigk---

---wvfstsllqflnf*fkkltlrkimplnfilgvhlpfqlsllnylllqsleqfvsfvl
fgqqvfitmvnmififi*lssssfsfvfhlvklylpfvsmlcwlkqfyhfy**cyfffve
ysflmnkfqtfgnsfiiqihavis*kvl*hqf*rmfllivqmki*pnsqtqqi*pvknis
sqpmemfvllpkamkvnvvivflnqvkniikllvglmktv*etmvfyglslfst*lgcfh
clfnkktk

Frame C:
GDMNFTCNDQTAIPNGNYIASNGSTMSYQDQYRACPSAGAIEGQMVNGEFYVAGSNYIDA
ALDFKSDDRTLNVIITFLWWIFFVIINMIALELFDWTSGGMPHKVYKRGKAPKINDDEEE
RQQNAMVENATSKMKDTLKMRESCFTWNHIHYTVQLNGKDLLLLNDVEGWIKPGQMTALM
GSSGAGKTTLLDVLA---

---gyslhlycnssifnsksll*erlcl*ilflvsichfnchc*itfccsrwnnlfllff
ldsryllqw*i*flflynfhplpfhlcftwsscicllfqcyvgsnnfttfisnvisflws
trsl*tnsklleirlsfksmplflrrccnisfkecfc*lfk*rfnqilkpnkfnl*rifq
anlwkcsccyqrr*k*mwllcf*ir*ril*nswlvl*kpfkklwyfmgflyfqhn*vvsi
vyltkkp
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI257 (VHI257Q) /CSM/VH/VHI2-C/VHI257Q.Seq.d/                       2563   0.0
VHI319 (VHI319Q) /CSM/VH/VHI3-A/VHI319Q.Seq.d/                       1429   0.0
VHD657 (VHD657Q) /CSM/VH/VHD6-C/VHD657Q.Seq.d/                       1419   0.0
VHB406 (VHB406Q) /CSM/VH/VHB4-A/VHB406Q.Seq.d/                       1419   0.0
VHA156 (VHA156Q) /CSM/VH/VHA1-C/VHA156Q.Seq.d/                       1413   0.0
SHF280 (SHF280Q) /CSM/SH/SHF2-D/SHF280Q.Seq.d/                       1413   0.0
AHH593 (AHH593Q) /CSM/AH/AHH5-D/AHH593Q.Seq.d/                       1413   0.0
VHK421 (VHK421Q) /CSM/VH/VHK4-A/VHK421Q.Seq.d/                       1411   0.0
CHF640 (CHF640Q) /CSM/CH/CHF6-B/CHF640Q.Seq.d/                       1411   0.0
SHJ852 (SHJ852Q) /CSM/SH/SHJ8-C/SHJ852Q.Seq.d/                       1407   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ441679) Dictyostelium discoideum cDNA clone:ddv47a16, 3' ...  1435   0.0    1
(BJ441553) Dictyostelium discoideum cDNA clone:ddv47f05, 3' ...  1429   0.0    1
(BJ438790) Dictyostelium discoideum cDNA clone:ddv38a16, 3' ...  1407   0.0    1
(BJ437086) Dictyostelium discoideum cDNA clone:ddv33l02, 3' ...  1407   0.0    1
(BJ407769) Dictyostelium discoideum cDNA clone:dds43h14, 3' ...  1407   0.0    1
(BJ352471) Dictyostelium discoideum cDNA clone:dda46i23, 3' ...  1407   0.0    1
(BJ442841) Dictyostelium discoideum cDNA clone:ddv51j06, 3' ...  1405   0.0    1
(BJ438352) Dictyostelium discoideum cDNA clone:ddv37m14, 3' ...  1405   0.0    1
(BJ437197) Dictyostelium discoideum cDNA clone:ddv33c13, 3' ...  1405   0.0    1
(BJ436610) Dictyostelium discoideum cDNA clone:ddv31o13, 3' ...  1402   0.0    1
dna update 2008.11.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF482389_1(AF482389|pid:none) Dictyostelium discoideum ABC trans...   426   e-117
AF482396_1(AF482396|pid:none) Dictyostelium discoideum ABC trans...   253   8e-66
AF482391_1(AF482391|pid:none) Dictyostelium discoideum ABC trans...   252   2e-65
AF482390_1(AF482390|pid:none) Dictyostelium discoideum ABC trans...   244   7e-63
AF037042_1(AF037042|pid:none) Dictyostelium discoideum ras-relat...   231   4e-59
AF482393_1(AF482393|pid:none) Dictyostelium discoideum ABC trans...   229   2e-58
AF482395_1(AF482395|pid:none) Dictyostelium discoideum ABC trans...   213   1e-53
AB091269_1(AB091269|pid:none) Magnaporthe grisea ABC2 gene for A...   114   1e-23
AM920427_990(AM920427|pid:none) Penicillium chrysogenum Wisconsi...   110   1e-22
CR382122_390(CR382122|pid:none) Kluyveromyces lactis strain NRRL...   110   1e-22
protein update 2009. 7.13
PSORT 

 psg: 0.75  gvh: 0.35  alm: 0.13  top: 0.63  tms: 0.40  mit: 0.16  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 1.00  m_ : 0.00 

	 56.0 %: plasma membrane
	 16.0 %: vacuolar
	 12.0 %: endoplasmic reticulum
	  8.0 %: mitochondrial
	  4.0 %: Golgi
	  4.0 %: nuclear

>> prediction for VHI257 is pla
5' end seq. ID VHI257F
5' end seq. 
>VHI257F.Seq
TTGGTGATATGAATTTCACATGTAATGATCAAACTGCAATTCCAAATGGTAATTACATTG
CATCTAATGGTAGTACAATGTCTTATCAAGATCAATATAGAGCTTGTCCATCTGCCGGTG
CTATCGAAGGTCAAATGGTTAATGGTGAATTTTATGTTGCTGGTTCAAACTATATAGATG
CTGCACTTGATTTCAAAAGTGATGACCGTACATTAAATGTCATTATCACTTTTTTATGGT
GGATATTTTTTGTAATTATCAATATGATTGCTTTGGAATTATTCGATTGGACAAGTGGTG
GTATGCCTCATAAAGTCTATAAAAGAGGAAAGGCTCCTAAAATCAACGATGACGAAGAAG
AGAGACAACAAAATGCAATGGTTGAAAATGCTACAAGTAAAATGAAAGATACTCTTAAAA
TGAGAGAAAGTTGTTTCACTTGGAATCATATTCACTATACCGTTCAATTAAATGGCAAAG
ATTTATTATTGTTAAATGATGTTGAAGGTTGGATTAAACCTGGTCAAATGACTGCATTAA
TGGGTTCATCTGGTGCGGGTAAAACAACATTACTCGATGTATTGGCAAANNNNNNNNNN
Length of 5' end seq. 599
3' end seq. ID VHI257Z
3' end seq. 
>VHI257Z.Seq
NNNNNNNNNNTTGGGTATTCTCTACATCTTTATTGCAATTCCTCAATTTTTAATTCAAAA
AGCTTACTTTAAGAAAGATTATGCCTCTAAATTTTATTCTTGGTGTCCATTTGCCATTTC
AATTGTCATTGTTGAATTACCTTTTGTTGCAGTCGCTGGAACAATTTGTTTCTTTTGTTC
TTTTTGGACAGCAGGTATTTATTACAATGGTGAATATGATTTTTATTTTTATATAACTTT
CATCCTCTTCCTTTTCATTTGTGTTTCACTTGGTCAAGTTGTATCTGCCTTTTGTTTCAA
TGTTATGTTGGCTCAAACAATTTTACCACTTTTATTAGTAATGTTATTTCTTTTTTGTGG
AGTACTCGTTCCTTATGAACAAATTCCAAACTTTTGGAAATTCGTTTATCATTCAAATCC
ATGCCGTTATTTCTTAGAAGGTGTTGTAACATCAGTTTTAAAGAATGTTTTTGTTGATTG
TTCAAATGAAGATTTAACCAAATTCTCAAACCCAACAAATTTAACCTGTAAAGAATATTT
CAAGCCAACCTATGGAAATGTTCGTGCTGTTACCAAAGGCGATGAAAGTGAATGTGGTTA
TTGTGTTTTTAAATCAGGTGAAGAATATTATAAAACTCTTGGTTGGTCTTATGAAAACCG
TTTAAGAAACTATGGTATTTTATGGGCTTTCTTTATTTTCAACATAATTAGGTTGTTTCC
ATTGTTTATTTAACAAAAAAACCAAA
Length of 3' end seq. 746
Connected seq. ID VHI257P
Connected seq. 
>VHI257P.Seq
TTGGTGATATGAATTTCACATGTAATGATCAAACTGCAATTCCAAATGGTAATTACATTG
CATCTAATGGTAGTACAATGTCTTATCAAGATCAATATAGAGCTTGTCCATCTGCCGGTG
CTATCGAAGGTCAAATGGTTAATGGTGAATTTTATGTTGCTGGTTCAAACTATATAGATG
CTGCACTTGATTTCAAAAGTGATGACCGTACATTAAATGTCATTATCACTTTTTTATGGT
GGATATTTTTTGTAATTATCAATATGATTGCTTTGGAATTATTCGATTGGACAAGTGGTG
GTATGCCTCATAAAGTCTATAAAAGAGGAAAGGCTCCTAAAATCAACGATGACGAAGAAG
AGAGACAACAAAATGCAATGGTTGAAAATGCTACAAGTAAAATGAAAGATACTCTTAAAA
TGAGAGAAAGTTGTTTCACTTGGAATCATATTCACTATACCGTTCAATTAAATGGCAAAG
ATTTATTATTGTTAAATGATGTTGAAGGTTGGATTAAACCTGGTCAAATGACTGCATTAA
TGGGTTCATCTGGTGCGGGTAAAACAACATTACTCGATGTATTGGCAAA----------T
TGGGTATTCTCTACATCTTTATTGCAATTCCTCAATTTTTAATTCAAAAAGCTTACTTTA
AGAAAGATTATGCCTCTAAATTTTATTCTTGGTGTCCATTTGCCATTTCAATTGTCATTG
TTGAATTACCTTTTGTTGCAGTCGCTGGAACAATTTGTTTCTTTTGTTCTTTTTGGACAG
CAGGTATTTATTACAATGGTGAATATGATTTTTATTTTTATATAACTTTCATCCTCTTCC
TTTTCATTTGTGTTTCACTTGGTCAAGTTGTATCTGCCTTTTGTTTCAATGTTATGTTGG
CTCAAACAATTTTACCACTTTTATTAGTAATGTTATTTCTTTTTTGTGGAGTACTCGTTC
CTTATGAACAAATTCCAAACTTTTGGAAATTCGTTTATCATTCAAATCCATGCCGTTATT
TCTTAGAAGGTGTTGTAACATCAGTTTTAAAGAATGTTTTTGTTGATTGTTCAAATGAAG
ATTTAACCAAATTCTCAAACCCAACAAATTTAACCTGTAAAGAATATTTCAAGCCAACCT
ATGGAAATGTTCGTGCTGTTACCAAAGGCGATGAAAGTGAATGTGGTTATTGTGTTTTTA
AATCAGGTGAAGAATATTATAAAACTCTTGGTTGGTCTTATGAAAACCGTTTAAGAAACT
ATGGTATTTTATGGGCTTTCTTTATTTTCAACATAATTAGGTTGTTTCCATTGTTTATTT
AACAAAAAAACCAAA
Length of connected seq. 1325
Full length Seq ID -
Full length Seq. -
Length of full length seq. -