>VHI253 (VHI253Q) /CSM/VH/VHI2-C/VHI253Q.Seq.d/
XXXXXXXXXXGNNGATTTANGTTCCCACANTGCCGGTCAAGCTTTCCCACAATGTGTGTT
NGATCATTGGGNTTCAATTGGTGTTGTCATCAAAGATAAGAAAGCCAGTGAAG

---XIXVPTXPVKLSHNVCXIIGXQLVLSSKIRKPVK

Frame A:
---XIXVPTXPVKLSHNVCXIIGXQLVLSSKIRKPVK


Frame B:
---xfxfpxcrssfptmcvxslgfnwcchqr*esq*


Frame C:
---dlxshxagqafpqcvxdhwxsigvvikdkkase

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI253 (VHI253Q) /CSM/VH/VHI2-C/VHI253Q.Seq.d/                        174   2e-43
VHP339 (VHP339Q) /CSM/VH/VHP3-B/VHP339Q.Seq.d/                        153   6e-37
VHN382 (VHN382Q) /CSM/VH/VHN3-D/VHN382Q.Seq.d/                        153   6e-37
VHM830 (VHM830Q) /CSM/VH/VHM8-B/VHM830Q.Seq.d/                        153   6e-37
VHM814 (VHM814Q) /CSM/VH/VHM8-A/VHM814Q.Seq.d/                        153   6e-37
VHM687 (VHM687Q) /CSM/VH/VHM6-D/VHM687Q.Seq.d/                        153   6e-37
VHM506 (VHM506Q) /CSM/VH/VHM5-A/VHM506Q.Seq.d/                        153   6e-37
VHL836 (VHL836Q) /CSM/VH/VHL8-B/VHL836Q.Seq.d/                        153   6e-37
VHL516 (VHL516Q) /CSM/VH/VHL5-A/VHL516Q.Seq.d/                        153   6e-37
VHL310 (VHL310Q) /CSM/VH/VHL3-A/VHL310Q.Seq.d/                        153   6e-37

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.   151   2e-33  1
CK273320|CK273320.1 EST719398 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POADA55 5' end, mRNA sequence.    72   2e-09  1
CK265485|CK265485.1 EST711563 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POABZ02 3' end, mRNA sequence.    72   2e-09  1
CK273321|CK273321.1 EST719399 potato abiotic stress cDNA library Solanum tuberosum cDNA clone POADA55 3' end, mRNA sequence.    72   2e-09  1
AU303519|AU303519.1 Zinnia elegans cDNA, clone:Z12358.             68   2e-08  1
BG524677|BG524677.1 5-70 Stevia field grown leaf cDNA Stevia rebaudiana cDNA 5', mRNA sequence.    68   2e-08  1
BM065200|BM065200.1 KS07001A06 KS07 Capsicum annuum cDNA, mRNA sequence.    68   2e-08  1
AJ299248|AJ299248.1 Nicotiana tabacum partial mRNA for elongation factor 2 (ef2 gene),  clone 3cT7.    68   2e-08  1
BG525773|BG525773.1 49-58 Stevia field grown leaf cDNA Stevia rebaudiana cDNA 5', mRNA sequence.    68   2e-08  1
CF096045|CF096045.1 QHN21O22.yg.ab1 QH_N sunflower H.argophyllus (drought stress) Helianthus argophyllus cDNA clone QHN21O22, mRNA sequence.    60   6e-06  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q06193) RecName: Full=Elongation factor 2;          Short=EF-2;...    40   0.026
AY831447_1(AY831447|pid:none) Monocercomonoides sp. PA203 transl...    40   0.034
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...    37   0.29
AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...    37   0.29
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...    37   0.29
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...    37   0.29
CR859604_1(CR859604|pid:none) Pongo abelii mRNA; cDNA DKFZp468K0...    37   0.29
DQ630927_1(DQ630927|pid:none) Gryllus pennsylvanicus elongation ...    37   0.29
DQ848932_1(DQ848932|pid:none) Scophthalmus maximus clone kbc101 ...    37   0.29
AK298498_1(AK298498|pid:none) Homo sapiens cDNA FLJ56548 complet...    37   0.29

 psg: 0.87  gvh: 0.50  alm: 0.43  top: 0.57  tms: 0.00  mit: 0.38  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: mitochondrial
	 24.0 %: cytoplasmic
	 24.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for VHI253 is mit

>VHI253Z.Seq
NNNNNNNNNNGNNGATTTANGTTCCCACANTGCCGGTCAAGCTTTCCCACAATGTGTGTT
NGATCATTGGGNTTCAATTGGTGTTGTCATCAAAGATAAGAAAGCCAGTGAAG