>AFO224 (AFO224Q) /CSM/AF/AFO2-A/AFO224Q.Seq.d/
TTTTAAAAAAAATCATTTGGGAAATTTAAAAATTTTTAATATAAAAAAATAAAAAAAATC
ATTTTTAACAACTTTTGTATACGCAGAAGAAAAAAAAAGAAATGAAAAATATATTATTAT
TCGGTTCTGGCTTTGTTGCAAAGCCAGCACTTGATTACCTCTTAAAAAGAGAAGATTATT
TTGTAACAATTGTTAGTTTATTCCAAAATGAATTAGACTCAATTACAAAAGGTCACGATA
CATCAAAATTCAAAACAATTCAATTAGATGTTATGAATAAATTGAATGAATTAGAAGAAT
ATTTCCCAAAATCTGATTGTGTAATTAGTTTAATTCCAGCAACATTACATTCAACAGTTG
CAAAATTATGTATTAAACATAAAACACATTTAGTTACAGCAAGTTATATTTCAGATGATA
TGAAAGCATTATCAGAAGAAGCAAAGGAGGCAGGTGTTTTATTATTGAATGAACTTGGTC
TTGATCCAGGTATTGATCATATGTCATCAATGAAGATTATTGATCATGCTAAGGAAAATG
GTGGTAAGGTCACATCATTCGTTTCAXXXXXXXXXXTAGAGGTACATTACGTTGGAAGGG
TGGTTTTGGAATCATGATCCGTGCACTTGTTGCCGTTGGTTTATTCTCCACTGAAGTTGA
TGCACGTCTCGCTGTTGAGGGTGGTATTTCATGGCGTAACTATCTCGTTCAGCTATTGGG
TTGCAATGATAATGATAGTGACCTCCTATATTGTGTGGAATCCACCATTAAAGAGTACTT
TGAAAAATTAAAGACTGAGAGAGACGGCTTACAATTCCATTTCCCAATTATTCCAAGAGA
TATTGAAAAAGATGTTCAACATGCCGTTGAAGGTTTCAAATGGTTGGGTTTATTAAGTGC
TGATGAAAAAGTTGTAAATAAGAATACTCCAATCGATAGTCTTTGCGCTTTACTCGAAAA
GAAACTCTCTTACAAAGCTGGTGAACGTGATGTAGTTGTCCTTGAACATAACTTTGTCGT
CCAATACGCTGATCGTACAGAAAAAGAAGTTTCATCACTCATTTGTTATGGTATTCCAAA
TGGTAGTTCTGCAACTTCACTCACTGTTGGTGTTCCAGTTGGTATCGCAACTGAACTCAT
CGCTGATGGTAAAACTACAACTAGAGGTGTTGTTGGTCCAGTAACTCCTGAATTTTATTT
ACCAATTTTAGAGAAATTAAAATCTAAAATATT

lkkiiwei*kfli*knkknhf*QLLYTQKKKKEMKNILLFGSGFVAKPALDYLLKREDYF
VTIVSLFQNELDSITKGHDTSKFKTIQLDVMNKLNELEEYFPKSDCVISLIPATLHSTVA
KLCIKHKTHLVTASYISDDMKALSEEAKEAGVLLLNELGLDPGIDHMSSMKIIDHAKENG
GKVTSFVS---

---RGTLRWKGGFGIMIRALVAVGLFSTEVDARLAVEGGISWRNYLVQLLGCNDNDSDLL
YCVESTIKEYFEKLKTERDGLQFHFPIIPRDIEKDVQHAVEGFKWLGLLSADEKVVNKNT
PIDSLCALLEKKLSYKAGERDVVVLEHNFVVQYADRTEKEVSSLICYGIPNGSSATSLTV
GVPVGIATELIADGKTTTRGVVGPVTPEFYLPILEKLKSKI

Frame A:
f*kksfgkfknf*ykkikkiifnnfcirrrkkkk*kiyyysvlallqsqhlits*kekii
l*qllvyskmn*tqlqkvtihqnskqfn*ml*in*mn*knisqnliv*lv*fqqhyiqql
qnyvlnikhi*lqqvifqmi*khyqkkqrrqvfyy*mnlvliqvliichq*rllimlrkm
vvrshhsf---

---*ryitlegwfwnhdpctccrwfilh*s*ctsrc*gwyfma*lsrsaiglq*****pp
ilcgihh*rvl*kikd*errltipfpnyskry*krcstcr*rfqmvgfikc**ksck*ey
snr*slrftrketllqsw*t*cscp*t*lcrpir*syrkrsfithllwyskw*fcnfthc
wcsswyrn*thr*w*nyn*rccwssns*ilftnfreiki*ni

Frame B:
fkknhlgnlkifnikk*kksflttfvyaeekkrnekyiiirfwlcckast*lplkkrrlf
cnnc*fipk*irlnykrsryikiqnnsircye*ie*irrifpki*lcn*fnssnitfnsc
kimy*t*ntfsysklyfr*yesiirrskggrcfiie*tws*sry*syvinedy*sc*gkw
w*ghiirf---

---RGTLRWKGGFGIMIRALVAVGLFSTEVDARLAVEGGISWRNYLVQLLGCNDNDSDLL
YCVESTIKEYFEKLKTERDGLQFHFPIIPRDIEKDVQHAVEGFKWLGLLSADEKVVNKNT
PIDSLCALLEKKLSYKAGERDVVVLEHNFVVQYADRTEKEVSSLICYGIPNGSSATSLTV
GVPVGIATELIADGKTTTRGVVGPVTPEFYLPILEKLKSKI

Frame C:
lkkiiwei*kfli*knkknhf*QLLYTQKKKKEMKNILLFGSGFVAKPALDYLLKREDYF
VTIVSLFQNELDSITKGHDTSKFKTIQLDVMNKLNELEEYFPKSDCVISLIPATLHSTVA
KLCIKHKTHLVTASYISDDMKALSEEAKEAGVLLLNELGLDPGIDHMSSMKIIDHAKENG
GKVTSFVS---

---evhyvgrvvles*svhllplvysplklmhvsllrvvfhgvtisfsywvamimivtsy
ivwnpplkstlkn*rlretaynsisqlfqeilkkmfnmplkvsngwvy*vlmkkl*iril
qsivfalyskrnsltklvnvm*lslnitlssntlivqkkkfhhsfvmvfqmvvlqlhsll
vfqlvsqlnsslmvklqlevllvq*llnfiyqf*rn*nlky

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO224 (AFO224Q) /CSM/AF/AFO2-A/AFO224Q.Seq.d/                       2216   0.0
AFH782 (AFH782Q) /CSM/AF/AFH7-D/AFH782Q.Seq.d/                       1298   0.0
VFM605 (VFM605Q) /CSM/VF/VFM6-A/VFM605Q.Seq.d/                       1294   0.0
VFJ362 (VFJ362Q) /CSM/VF/VFJ3-C/VFJ362Q.Seq.d/                       1294   0.0
CFI658 (CFI658Q) /CSM/CF/CFI6-C/CFI658Q.Seq.d/                       1294   0.0
AFF496 (AFF496Q) /CSM/AF/AFF4-D/AFF496Q.Seq.d/                       1294   0.0
AFO144 (AFO144Q) /CSM/AF/AFO1-B/AFO144Q.Seq.d/                       1292   0.0
AFO793 (AFO793Q) /CSM/AF/AFO7-D/AFO793Q.Seq.d/                       1291   0.0
SFE805 (SFE805Q) /CSM/SF/SFE8-A/SFE805Q.Seq.d/                       1289   0.0
VFF694 (VFF694Q) /CSM/VF/VFF6-D/VFF694Q.Seq.d/                       1287   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AL394098|AL394098.1 T3 end of clone AR0AA015A07 of library AR0AA from strain CBS 732 of Zygosaccharomyces rouxii.    46  9e-04  2
AX573242|AX573242.1 Sequence 88 from Patent WO02074992.           46  0.18  4
AL499626|AL499626.5 Human DNA sequence from clone RP3-381A2 on chromosome 6.    48  0.26  1
AC021509|AC021509.6 Homo sapiens chromosome 18 clone RP11-255L11 map 18, WORKING DRAFT SEQUENCE, 3 unordered pieces.    40  0.39  7
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    34  0.51  12
AE010592|AE010592.1 Fusobacterium nucleatum subsp. nucleatum ATCC 25586, section 134 of 197 of the complete genome.    34  0.77  4
AP004531|AP004531.1 Lotus japonicus genomic DNA, chromosome 3, clone:LjT13M14, TM0059.    48  0.85  4
AL591849|AL591849.7 Human DNA sequence from clone RP11-321G1 on chromosome Xq22.3-24.    36  0.88  3
AC022375|AC022375.2 Homo sapiens chromosome 3, WORKING DRAFT SEQUENCE, 21 unordered pieces.    46  1.0  1
BB826079|BB826079.1 Mus musculus mammary gland RCB-0526 Jyg-MC(A) cDNA, RIKEN full-length enriched library, clone:G830041E09, 3' end partial sequence.    34  1.1  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54NG9) RecName: Full=Probable saccharopine dehydrogenase [NADP...   405   e-111
BC073642_1(BC073642|pid:none) Xenopus laevis MGC82978 protein, m...   132   2e-29
FM992690_618(FM992690|pid:none) Candida dubliniensis CD36 chromo...   128   3e-28
AM989986_8(AM989986|pid:none) Zygosaccharomyces rouxii strain CB...   125   3e-27
(A2VCW9) RecName: Full=Alpha-aminoadipic semialdehyde synthase, ...   124   8e-27
(A8E657) RecName: Full=Alpha-aminoadipic semialdehyde synthase, ...   124   8e-27
CR382130_918(CR382130|pid:none) Yarrowia lipolytica strain CLIB1...   123   1e-26
AM398681_2309(AM398681|pid:none) Flavobacterium psychrophilum JI...   123   1e-26
AY751465_1(AY751465|pid:none) Oncorhynchus mykiss lysine ketoglu...   122   2e-26
(O59711) RecName: Full=Saccharopine dehydrogenase [NADP+, L-glut...   121   4e-26

 psg: 0.79  gvh: 0.46  alm: 0.33  top: 0.53  tms: 0.00  mit: 0.17  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.40  pox: 0.30  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 24.0 %: nuclear
	 16.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: endoplasmic reticulum
	  4.0 %: peroxisomal

>> prediction for AFO224 is cyt

>AFO224F.Seq
TTTTAAAAAAAATCATTTGGGAAATTTAAAAATTTTTAATATAAAAAAATAAAAAAAATC
ATTTTTAACAACTTTTGTATACGCAGAAGAAAAAAAAAGAAATGAAAAATATATTATTAT
TCGGTTCTGGCTTTGTTGCAAAGCCAGCACTTGATTACCTCTTAAAAAGAGAAGATTATT
TTGTAACAATTGTTAGTTTATTCCAAAATGAATTAGACTCAATTACAAAAGGTCACGATA
CATCAAAATTCAAAACAATTCAATTAGATGTTATGAATAAATTGAATGAATTAGAAGAAT
ATTTCCCAAAATCTGATTGTGTAATTAGTTTAATTCCAGCAACATTACATTCAACAGTTG
CAAAATTATGTATTAAACATAAAACACATTTAGTTACAGCAAGTTATATTTCAGATGATA
TGAAAGCATTATCAGAAGAAGCAAAGGAGGCAGGTGTTTTATTATTGAATGAACTTGGTC
TTGATCCAGGTATTGATCATATGTCATCAATGAAGATTATTGATCATGCTAAGGAAAATG
GTGGTAAGGTCACATCATTCGTTTCA----------

>AFO224Z.Seq
----------TAGAGGTACATTACGTTGGAAGGGTGGTTTTGGAATCATGATCCGTGCAC
TTGTTGCCGTTGGTTTATTCTCCACTGAAGTTGATGCACGTCTCGCTGTTGAGGGTGGTA
TTTCATGGCGTAACTATCTCGTTCAGCTATTGGGTTGCAATGATAATGATAGTGACCTCC
TATATTGTGTGGAATCCACCATTAAAGAGTACTTTGAAAAATTAAAGACTGAGAGAGACG
GCTTACAATTCCATTTCCCAATTATTCCAAGAGATATTGAAAAAGATGTTCAACATGCCG
TTGAAGGTTTCAAATGGTTGGGTTTATTAAGTGCTGATGAAAAAGTTGTAAATAAGAATA
CTCCAATCGATAGTCTTTGCGCTTTACTCGAAAAGAAACTCTCTTACAAAGCTGGTGAAC
GTGATGTAGTTGTCCTTGAACATAACTTTGTCGTCCAATACGCTGATCGTACAGAAAAAG
AAGTTTCATCACTCATTTGTTATGGTATTCCAAATGGTAGTTCTGCAACTTCACTCACTG
TTGGTGTTCCAGTTGGTATCGCAACTGAACTCATCGCTGATGGTAAAACTACAACTAGAG
GTGTTGTTGGTCCAGTAACTCCTGAATTTTATTTACCAATTTTAGAGAAATTAAAATCTA
AAATATT

>AFO224P.Seq
TTTTAAAAAAAATCATTTGGGAAATTTAAAAATTTTTAATATAAAAAAATAAAAAAAATC
ATTTTTAACAACTTTTGTATACGCAGAAGAAAAAAAAAGAAATGAAAAATATATTATTAT
TCGGTTCTGGCTTTGTTGCAAAGCCAGCACTTGATTACCTCTTAAAAAGAGAAGATTATT
TTGTAACAATTGTTAGTTTATTCCAAAATGAATTAGACTCAATTACAAAAGGTCACGATA
CATCAAAATTCAAAACAATTCAATTAGATGTTATGAATAAATTGAATGAATTAGAAGAAT
ATTTCCCAAAATCTGATTGTGTAATTAGTTTAATTCCAGCAACATTACATTCAACAGTTG
CAAAATTATGTATTAAACATAAAACACATTTAGTTACAGCAAGTTATATTTCAGATGATA
TGAAAGCATTATCAGAAGAAGCAAAGGAGGCAGGTGTTTTATTATTGAATGAACTTGGTC
TTGATCCAGGTATTGATCATATGTCATCAATGAAGATTATTGATCATGCTAAGGAAAATG
GTGGTAAGGTCACATCATTCGTTTCA----------TAGAGGTACATTACGTTGGAAGGG
TGGTTTTGGAATCATGATCCGTGCACTTGTTGCCGTTGGTTTATTCTCCACTGAAGTTGA
TGCACGTCTCGCTGTTGAGGGTGGTATTTCATGGCGTAACTATCTCGTTCAGCTATTGGG
TTGCAATGATAATGATAGTGACCTCCTATATTGTGTGGAATCCACCATTAAAGAGTACTT
TGAAAAATTAAAGACTGAGAGAGACGGCTTACAATTCCATTTCCCAATTATTCCAAGAGA
TATTGAAAAAGATGTTCAACATGCCGTTGAAGGTTTCAAATGGTTGGGTTTATTAAGTGC
TGATGAAAAAGTTGTAAATAAGAATACTCCAATCGATAGTCTTTGCGCTTTACTCGAAAA
GAAACTCTCTTACAAAGCTGGTGAACGTGATGTAGTTGTCCTTGAACATAACTTTGTCGT
CCAATACGCTGATCGTACAGAAAAAGAAGTTTCATCACTCATTTGTTATGGTATTCCAAA
TGGTAGTTCTGCAACTTCACTCACTGTTGGTGTTCCAGTTGGTATCGCAACTGAACTCAT
CGCTGATGGTAAAACTACAACTAGAGGTGTTGTTGGTCCAGTAACTCCTGAATTTTATTT
ACCAATTTTAGAGAAATTAAAATCTAAAATATT