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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI230
Library VH
(Link to library)
Clone ID VHI230
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16423-1
Original site URL
Representative seq. ID VHI230P
(Link to Original site)
Representative DNA sequence 
>VHI230 (VHI230Q) /CSM/VH/VHI2-B/VHI230Q.Seq.d/
AATTTTCATCCACGCGGATAAATTTTTTTAAAGAATTAGTCATTTTATGCCAATTGGAAA
TAACAAAGATAACGATGGAACTACCCTGAATATCTCAGGGTTAACCATCTCTGATAAGAG
TTCGTCAACTTCATCAAACAACAACACAACAACAACAACAACACAAACTAGTGAACCATA
TGTCCCACCATCTCGTAGAAACAAGATGAATAATAGTTCAGGTTCATCAAGAGACGATTA
TCCATCACCATCTGACCGTGATAATCGTGATTCACCATTCAATCGTGACCGXXXXXXXXX
XGTTGAAACTAAACGTTCTTGTGATACTCTTACCTACTTTTTAAATCAAAGAAATTTCCC
AACTACCTGTATTCATGGTGATTTAACTCAACCAGAAAGAGAAAATGCACTTCAATCTTT
CCGTTCTTTTGCAACTCCATTCTTAGTAGCAACTGATATTGCATCAAGAGGTCTCCACAT
CGGTAATGTTAATTTGGTTATCAATTTCGACTTGCCTACCGACATACATATCTACGTTCA
TCGTATTGGTAGAACCGGAAGAGCAGGAAAAAAAGGTTTAGCAATTTCCTTCTTCAATGA
AAAGAATAAACCAGTTGGTGCTGAACTTTTGAAATTAATGAAAGCTTCAAATCAAGATAT
TCCAGATTGGTTTGAAAAGATGGTACACAATCTCCGTATGTCTAAAGGTCCATCAAACTC
AAAATCAAATAGTCCATTCAACAAATCTTACAATAGTCATCACAATAGAGATGATAACCG
TGGTGGTTATGGTGGTGGTGGTGGTGGTAATGGAGGTTACAGTAACAACCGTAATGATAG
AAGAGAAGATAAATCAGATTACTCAATGCATCACCCATACTTTAGCAACAATGGTGGTTC
ATATAATTAAACATTCACCTCATGTCATTCGTTTAATAAAAAATAATAATCCATTTNATT
CAATAT
sequence update 2002.10.25
Translated Amino Acid sequence 
ifihadkff*RISHFMPIGNNKDNDGTTLNISGLTISDKSSSTSSNNNTTTTTTQTSEPY
VPPSRRNKMNNSSGSSRDDYPSPSDRDNRDSPFNRD---

---VETKRSCDTLTYFLNQRNFPTTCIHGDLTQPERENALQSFRSFATPFLVATDIASRG
LHIGNVNLVINFDLPTDIHIYVHRIGRTGRAGKKGLAISFFNEKNKPVGAELLKLMKASN
QDIPDWFEKMVHNLRMSKGPSNSKSNSPFNKSYNSHHNRDDNRGGYGGGGGGNGGYSNNR
NDRREDKSDYSMHHPYFSNNGGSYN*tftschsfnkk**sixfn
Translated Amino Acid sequence (All Frames) 
Frame A:
nfhprg*iflkn*sfyanwk*qr*rwnypeylrvnhl**efvnfikqqhnnnnntn**ti
cptis*kqde**frfikrrlsiti*p**s*ftiqs*p---

---VETKRSCDTLTYFLNQRNFPTTCIHGDLTQPERENALQSFRSFATPFLVATDIASRG
LHIGNVNLVINFDLPTDIHIYVHRIGRTGRAGKKGLAISFFNEKNKPVGAELLKLMKASN
QDIPDWFEKMVHNLRMSKGPSNSKSNSPFNKSYNSHHNRDDNRGGYGGGGGGNGGYSNNR
NDRREDKSDYSMHHPYFSNNGGSYN*tftschsfnkk**sixfn

Frame B:
ifihadkff*RISHFMPIGNNKDNDGTTLNISGLTISDKSSSTSSNNNTTTTTTQTSEPY
VPPSRRNKMNNSSGSSRDDYPSPSDRDNRDSPFNRD---

---lklnvlvillptf*ikeisqlpvfmvi*lnqkekmhfnlsvllqlhs**qlilhqev
stsvmliwlsistclptyistfivlvepeeqekkv*qfpssmkrinqlvlnf*n**klqi
kifqiglkrwytisvclkvhqtqnqivhstnltivitiemitvvvmvvvvvvmevtvttv
mieekinqitqcithtlatmvvhiikhsphvirliknnnpfxsi

Frame C:
fsstrinffkelvilcqleitkitmelp*isqg*pslirvrqlhqtttqqqqqhklvnhm
shhlvetr*iivqvhqetiihhhltviivihhsivt---

---*n*tfl*ysyllfkskkfpnylysw*fnstrkrkctsifpffcnsilssn*ycikrs
phr*c*fgyqfrlayrhtylrssyw*nrksrkkrfsnfllq*ke*tswc*tfeinesfks
rysrlv*kdgtqspyv*rsiklkik*siqqilq*ssq*r**pwwlwwwwww*wrlq*qp*
**krr*irllnaspil*qqwwfi*lnihlmsfv**kiiihxiqy
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI230 (VHI230Q) /CSM/VH/VHI2-B/VHI230Q.Seq.d/                       1110   0.0
VHQ404 (VHQ404Q) /CSM/VH/VHQ4-A/VHQ404Q.Seq.d/                        906   0.0
VHP734 (VHP734Q) /CSM/VH/VHP7-B/VHP734Q.Seq.d/                        906   0.0
VHP681 (VHP681Q) /CSM/VH/VHP6-D/VHP681Q.Seq.d/                        906   0.0
VHP588 (VHP588Q) /CSM/VH/VHP5-D/VHP588Q.Seq.d/                        906   0.0
VHP118 (VHP118Q) /CSM/VH/VHP1-A/VHP118Q.Seq.d/                        906   0.0
VHO596 (VHO596Q) /CSM/VH/VHO5-D/VHO596Q.Seq.d/                        906   0.0
VHM740 (VHM740Q) /CSM/VH/VHM7-B/VHM740Q.Seq.d/                        906   0.0
VHL839 (VHL839Q) /CSM/VH/VHL8-B/VHL839Q.Seq.d/                        906   0.0
VHL166 (VHL166Q) /CSM/VH/VHL1-C/VHL166Q.Seq.d/                        906   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ446556) Dictyostelium discoideum cDNA clone:ddv63h02, 3' ...   525   0.0    3
(BJ446510) Dictyostelium discoideum cDNA clone:ddv62o21, 3' ...   525   0.0    3
(BJ446439) Dictyostelium discoideum cDNA clone:ddv62a22, 3' ...   525   0.0    3
(BJ446293) Dictyostelium discoideum cDNA clone:ddv62d09, 3' ...   525   0.0    3
(BJ445908) Dictyostelium discoideum cDNA clone:ddv61c05, 3' ...   525   0.0    3
(BJ445889) Dictyostelium discoideum cDNA clone:ddv60o23, 3' ...   525   0.0    3
(BJ445008) Dictyostelium discoideum cDNA clone:ddv58k06, 3' ...   525   0.0    3
(BJ444467) Dictyostelium discoideum cDNA clone:ddv56p09, 3' ...   525   0.0    3
(BJ444461) Dictyostelium discoideum cDNA clone:ddv56o08, 3' ...   525   0.0    5
(BJ444269) Dictyostelium discoideum cDNA clone:ddv55g21, 3' ...   525   0.0    4
dna update 2008.11.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54QS3) RecName: Full=Probable ATP-dependent RNA helicase ddx3;...   310   5e-83
(Q6Z4K6) RecName: Full=DEAD-box ATP-dependent RNA helicase 52B; ...   156   9e-37
BT085991_1(BT085991|pid:none) Zea mays full-length cDNA clone ZM...   152   1e-35
(Q75HJ0) RecName: Full=DEAD-box ATP-dependent RNA helicase 37;  ...   152   2e-35
FN357514_12(FN357514|pid:none) Schistosoma mansoni genome sequen...   152   2e-35
EU953399_1(EU953399|pid:none) Zea mays clone 1406878 unknown mRNA.    152   2e-35
(Q2R1M8) RecName: Full=DEAD-box ATP-dependent RNA helicase 52C; ...   150   8e-35
AY062446_1(AY062446|pid:none) Arabidopsis thaliana ATP-dependent...   149   1e-34
AM048814_1(AM048814|pid:none) Platynereis dumerilii mRNA for PL1...   149   1e-34
AM444101_1(AM444101|pid:none) Vitis vinifera contig VV78X103110....   149   2e-34
protein update 2009. 7.13
PSORT 

 psg: 0.88  gvh: 0.39  alm: 0.46  top: 0.30  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.08  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 44.0 %: nuclear
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VHI230 is cyt
5' end seq. ID VHI230F
5' end seq. 
>VHI230F.Seq
AATTTTCATCCACGCGGATAAATTTTTTTAAAGAATTAGTCATTTTATGCCAATTGGAAA
TAACAAAGATAACGATGGAACTACCCTGAATATCTCAGGGTTAACCATCTCTGATAAGAG
TTCGTCAACTTCATCAAACAACAACACAACAACAACAACAACACAAACTAGTGAACCATA
TGTCCCACCATCTCGTAGAAACAAGATGAATAATAGTTCAGGTTCATCAAGAGACGATTA
TCCATCACCATCTGACCGTGATAATCGTGATTCACCATTCAATCGTGACCGNNNNNNNNN
N
Length of 5' end seq. 301
3' end seq. ID VHI230Z
3' end seq. 
>VHI230Z.Seq
NNNNNNNNNNGTTGAAACTAAACGTTCTTGTGATACTCTTACCTACTTTTTAAATCAAAG
AAATTTCCCAACTACCTGTATTCATGGTGATTTAACTCAACCAGAAAGAGAAAATGCACT
TCAATCTTTCCGTTCTTTTGCAACTCCATTCTTAGTAGCAACTGATATTGCATCAAGAGG
TCTCCACATCGGTAATGTTAATTTGGTTATCAATTTCGACTTGCCTACCGACATACATAT
CTACGTTCATCGTATTGGTAGAACCGGAAGAGCAGGAAAAAAAGGTTTAGCAATTTCCTT
CTTCAATGAAAAGAATAAACCAGTTGGTGCTGAACTTTTGAAATTAATGAAAGCTTCAAA
TCAAGATATTCCAGATTGGTTTGAAAAGATGGTACACAATCTCCGTATGTCTAAAGGTCC
ATCAAACTCAAAATCAAATAGTCCATTCAACAAATCTTACAATAGTCATCACAATAGAGA
TGATAACCGTGGTGGTTATGGTGGTGGTGGTGGTGGTAATGGAGGTTACAGTAACAACCG
TAATGATAGAAGAGAAGATAAATCAGATTACTCAATGCATCACCCATACTTTAGCAACAA
TGGTGGTTCATATAATTAAACATTCACCTCATGTCATTCGTTTAATAAAAAATAATAATC
CATTTNATTCAATAT
Length of 3' end seq. 675
Connected seq. ID VHI230P
Connected seq. 
>VHI230P.Seq
AATTTTCATCCACGCGGATAAATTTTTTTAAAGAATTAGTCATTTTATGCCAATTGGAAA
TAACAAAGATAACGATGGAACTACCCTGAATATCTCAGGGTTAACCATCTCTGATAAGAG
TTCGTCAACTTCATCAAACAACAACACAACAACAACAACAACACAAACTAGTGAACCATA
TGTCCCACCATCTCGTAGAAACAAGATGAATAATAGTTCAGGTTCATCAAGAGACGATTA
TCCATCACCATCTGACCGTGATAATCGTGATTCACCATTCAATCGTGACCG---------
-GTTGAAACTAAACGTTCTTGTGATACTCTTACCTACTTTTTAAATCAAAGAAATTTCCC
AACTACCTGTATTCATGGTGATTTAACTCAACCAGAAAGAGAAAATGCACTTCAATCTTT
CCGTTCTTTTGCAACTCCATTCTTAGTAGCAACTGATATTGCATCAAGAGGTCTCCACAT
CGGTAATGTTAATTTGGTTATCAATTTCGACTTGCCTACCGACATACATATCTACGTTCA
TCGTATTGGTAGAACCGGAAGAGCAGGAAAAAAAGGTTTAGCAATTTCCTTCTTCAATGA
AAAGAATAAACCAGTTGGTGCTGAACTTTTGAAATTAATGAAAGCTTCAAATCAAGATAT
TCCAGATTGGTTTGAAAAGATGGTACACAATCTCCGTATGTCTAAAGGTCCATCAAACTC
AAAATCAAATAGTCCATTCAACAAATCTTACAATAGTCATCACAATAGAGATGATAACCG
TGGTGGTTATGGTGGTGGTGGTGGTGGTAATGGAGGTTACAGTAACAACCGTAATGATAG
AAGAGAAGATAAATCAGATTACTCAATGCATCACCCATACTTTAGCAACAATGGTGGTTC
ATATAATTAAACATTCACCTCATGTCATTCGTTTAATAAAAAATAATAATCCATTTNATT
CAATAT
Length of connected seq. 956
Full length Seq ID -
Full length Seq. -
Length of full length seq. -