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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI227
Library VH
(Link to library)
Clone ID VHI227
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHI227P
(Link to Original site)
Representative DNA sequence 
>VHI227 (VHI227Q) /CSM/VH/VHI2-B/VHI227Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGAXXXXXXXXXXATCTCGCTGATAACCACGAATGGGATAAAAATGATGCCATGAA
CATTTGGTCTTTCGGTCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGG
TGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAA
AGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCAC
CCTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGT
ACTCTATGCTGCTGAATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGA
AATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGG
TATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTT
ACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGC
TTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAA
AGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGA
TCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTNAGTTTTCAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTE---

---LADNHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGV
VCDENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPTLLEPIYLVEITA
PENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQ
CVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti*valxfs
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlk---

---islittngikmmp*tfglsvqkvtvpisssmspkvfni*mklkilslvlsngppkkv
lfvmkt*evsvsifmmspstlmpstevvvkssqllvvysmlln*lppqls*nqst**kll
pqktllvvftlysivvevlllvknvvsvphyslskliyqy*npsvspliyvptlpvklsh
nvssiiglqlvlstkirkplksl*pperekv*pqksqiltnstknskqfkshxsfq

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*h*r---

---sr**prmg*k*chehlvfrsrr*rcqsprqchqrcsife*n*rffrwcfpmghqrrc
cl**kherypfqsl*chpph*chpqrwwsnhpncssctlcc*inclpnslrtnllsrnyc
prkrywwyllctqss*rycyw*rtsyrfpiilcqssftsirilrfhr*ftfphcrssfpt
mclrslgfnwccqqr*esh*srfshqkekrfsprnprs*qiprktlnnlsrtxvfk

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTE---

---LADNHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGV
VCDENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPTLLEPIYLVEITA
PENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQ
CVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti*valxfs
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ491 (VHQ491Q) /CSM/VH/VHQ4-D/VHQ491Q.Seq.d/                       1382   0.0
VHQ483 (VHQ483Q) /CSM/VH/VHQ4-D/VHQ483Q.Seq.d/                       1382   0.0
VHQ476 (VHQ476Q) /CSM/VH/VHQ4-D/VHQ476Q.Seq.d/                       1382   0.0
VHQ473 (VHQ473Q) /CSM/VH/VHQ4-D/VHQ473Q.Seq.d/                       1382   0.0
VHQ453 (VHQ453Q) /CSM/VH/VHQ4-C/VHQ453Q.Seq.d/                       1382   0.0
VHQ434 (VHQ434Q) /CSM/VH/VHQ4-B/VHQ434Q.Seq.d/                       1382   0.0
VHQ410 (VHQ410Q) /CSM/VH/VHQ4-A/VHQ410Q.Seq.d/                       1382   0.0
VHQ359 (VHQ359Q) /CSM/VH/VHQ3-C/VHQ359Q.Seq.d/                       1382   0.0
VHQ346 (VHQ346Q) /CSM/VH/VHQ3-B/VHQ346Q.Seq.d/                       1382   0.0
VHQ323 (VHQ323Q) /CSM/VH/VHQ3-A/VHQ323Q.Seq.d/                       1382   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ446824) Dictyostelium discoideum cDNA clone:ddv63o22, 3' ...  1382   0.0    1
(BJ446813) Dictyostelium discoideum cDNA clone:ddv63m22, 3' ...  1382   0.0    1
(BJ446806) Dictyostelium discoideum cDNA clone:ddv63k22, 3' ...  1382   0.0    1
(BJ446805) Dictyostelium discoideum cDNA clone:ddv63k21, 3' ...  1382   0.0    1
(BJ446797) Dictyostelium discoideum cDNA clone:ddv63i24, 3' ...  1382   0.0    1
(BJ446787) Dictyostelium discoideum cDNA clone:ddv63h20, 3' ...  1382   0.0    1
(BJ446785) Dictyostelium discoideum cDNA clone:ddv63g24, 3' ...  1382   0.0    1
(BJ446779) Dictyostelium discoideum cDNA clone:ddv63f24, 3' ...  1382   0.0    1
(BJ446777) Dictyostelium discoideum cDNA clone:ddv63f22, 3' ...  1382   0.0    1
(BJ446756) Dictyostelium discoideum cDNA clone:ddv63b20, 3' ...  1382   0.0    1
dna update 2008.11.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   291   3e-77
BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   289   2e-76
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   289   2e-76
BT059038_1(BT059038|pid:none) Salmo salar clone ssal-rgf-521-328...   289   2e-76
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   288   3e-76
BT072304_1(BT072304|pid:none) Salmo salar clone ssal-rgf-520-280...   288   4e-76
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   287   7e-76
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   285   3e-75
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   285   3e-75
AK298498_1(AK298498|pid:none) Homo sapiens cDNA FLJ56548 complet...   285   3e-75
protein update 2009. 7.13
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHI227 is cyt
5' end seq. ID VHI227F
5' end seq. 
>VHI227F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGANNNNNNNNNN
Length of 5' end seq. 617
3' end seq. ID VHI227Z
3' end seq. 
>VHI227Z.Seq
NNNNNNNNNNATCTCGCTGATAACCACGAATGGGATAAAAATGATGCCATGAACATTTGG
TCTTTCGGTCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAA
TATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGT
GTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCAC
ACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTAT
GCTGCTGAATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACT
GCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTT
ATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTA
TTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCA
CAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACT
GAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGAC
AAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTNAGTTTTCAAA
Length of 3' end seq. 710
Connected seq. ID VHI227P
Connected seq. 
>VHI227P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGA----------ATCTCGCTGATAACCACGAATGGGATAAAAATGATGCCATGAA
CATTTGGTCTTTCGGTCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGG
TGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAA
AGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCAC
CCTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGT
ACTCTATGCTGCTGAATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGA
AATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGG
TATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTT
ACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGC
TTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAA
AGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGA
TCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTNAGTTTTCAAA
Length of connected seq. 1307
Full length Seq ID -
Full length Seq. -
Length of full length seq. -