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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI223
Library VH
(Link to library)
Clone ID VHI223
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHI223P
(Link to Original site)
Representative DNA sequence 
>VHI223 (VHI223Q) /CSM/VH/VHI2-A/VHI223Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATATGGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACT
GAAGAAGCTTATCTCTCTTTXXXXXXXXXXTCCAGAAGGTAACGGTGCCAATCTCCTCGT
CAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTT
CCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAA
TCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCC
AACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTNTNTTAGAACC
AATNTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACT
CAATCGTCGTANAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTC
TGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCA
CACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGT
CAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGC
CCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACT
TTGAGTTTTCAAAAATAGNAATCAAATCTAAAAAAAATTTTAAAAAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*SLSIASKVFAYKPKLYYVKPSLNVSNQSYMVNKVDRFLLELQLNT
EEAYLS---

---PEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLH
TDAIHRGGGQIIPTARRVLYAAELTASPTXLEPXYLVEITAPENAIGGIYSVLNRRXGIV
IGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKAT
EVALATRKRKGLAPEIPDLDKFHEKL*ti*val*vfknxnqi*kkf*kk
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*SLSIASKVFAYKPKLYYVKPSLNVSNQSYMVNKVDRFLLELQLNT
EEAYLS---

---srr*rcqsprqchqrcsife*n*rffrwcfpmghqrrccl**kherypfqsl*chpp
h*chpqrwwsnhpncssctlcc*inclpnxxrtnxlsrnycprkrywwyllctqssxryc
yw*rtsyrfpiilcqssftsirilrfhr*ftfphcrssfptmclrslgfnwccqqr*esh
*srfshqkekrfsprnprs*qiprktlnnlsrtlsfqk*xsnlkkilkkk

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtsliwstksivsy*nsn*tl
kklisl---

---PEGNGANLLVNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLH
TDAIHRGGGQIIPTARRVLYAAELTASPTXLEPXYLVEITAPENAIGGIYSVLNRRXGIV
IGEERRIGSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKAT
EVALATRKRKGLAPEIPDLDKFHEKL*ti*val*vfknxnqi*kkf*kk

Frame C:
lssakkvkykmvnftidqiraimdrrenirnmsviahvdhgkttlsdsliqragiiadkv
sgdmrymscradeqergitiksssvslhfempkedklpagctsheflinlidspghvdfs
sevtaalrvtdgalvvidcvegvcvqtetvlrqavaerikpvlygqqsrsflirtpikh*
rslslf---

---qkvtvpisssmspkvfni*mklkilslvlsngppkkvlfvmkt*evsvsifmmspst
lmpstevvvkssqllvvysmlln*lppqlx*nqxt**kllpqktllvvftlysivvxvll
lvknvvsvphyslskliyqy*npsvspliyvptlpvklshnvssiiglqlvlstkirkpl
ksl*pperekv*pqksqiltnstknskqfkshfefskixikskknfkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI223 (VHI223Q) /CSM/VH/VHI2-A/VHI223Q.Seq.d/                       2307   0.0
VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                       1277   0.0
VHP832 (VHP832Q) /CSM/VH/VHP8-B/VHP832Q.Seq.d/                       1277   0.0
VHP776 (VHP776Q) /CSM/VH/VHP7-D/VHP776Q.Seq.d/                       1277   0.0
VHP213 (VHP213Q) /CSM/VH/VHP2-A/VHP213Q.Seq.d/                       1277   0.0
VHP166 (VHP166Q) /CSM/VH/VHP1-C/VHP166Q.Seq.d/                       1277   0.0
VHO565 (VHO565Q) /CSM/VH/VHO5-C/VHO565Q.Seq.d/                       1277   0.0
VHK684 (VHK684Q) /CSM/VH/VHK6-D/VHK684Q.Seq.d/                       1277   0.0
VHK214 (VHK214Q) /CSM/VH/VHK2-A/VHK214Q.Seq.d/                       1277   0.0
VHJ652 (VHJ652Q) /CSM/VH/VHJ6-C/VHJ652Q.Seq.d/                       1277   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ446473) Dictyostelium discoideum cDNA clone:ddv62h19, 3' ...  1277   0.0    1
(BJ446358) Dictyostelium discoideum cDNA clone:ddv62p08, 3' ...  1277   0.0    1
(BJ446062) Dictyostelium discoideum cDNA clone:ddv61c17, 3' ...  1277   0.0    1
(BJ445933) Dictyostelium discoideum cDNA clone:ddv61i04, 3' ...  1277   0.0    1
(BJ445738) Dictyostelium discoideum cDNA clone:ddv60a17, 3' ...  1277   0.0    1
(BJ443409) Dictyostelium discoideum cDNA clone:ddv52g22, 3' ...  1277   0.0    1
(BJ442845) Dictyostelium discoideum cDNA clone:ddv51k04, 3' ...  1277   0.0    1
(BJ442655) Dictyostelium discoideum cDNA clone:ddv50g14, 3' ...  1277   0.0    1
(BJ441752) Dictyostelium discoideum cDNA clone:ddv47a24, 3' ...  1277   0.0    1
(BJ441605) Dictyostelium discoideum cDNA clone:ddv47a12, 3' ...  1277   0.0    1
dna update 2008.11.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   263   7e-69
DQ848932_1(DQ848932|pid:none) Scophthalmus maximus clone kbc101 ...   262   2e-68
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   261   5e-68
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   259   1e-67
A40411(A40411) translation elongation factor eEF-2 - Caenorhabdi...   259   1e-67
BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   259   1e-67
BT059038_1(BT059038|pid:none) Salmo salar clone ssal-rgf-521-328...   259   1e-67
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   258   2e-67
BT072304_1(BT072304|pid:none) Salmo salar clone ssal-rgf-520-280...   258   3e-67
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   257   5e-67
protein update 2009. 7.13
PSORT 

 psg: 0.67  gvh: 0.40  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: nuclear
	 28.0 %: cytoplasmic
	 20.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for VHI223 is nuc
5' end seq. ID VHI223F
5' end seq. 
>VHI223F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATATGGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACT
GAAGAAGCTTATCTCTCTTTNNNNNNNNNN
Length of 5' end seq. 570
3' end seq. ID VHI223Z
3' end seq. 
>VHI223Z.Seq
NNNNNNNNNNTCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTC
AATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAG
GTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCC
ACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCT
ATGCTGCTGAATTAACTGCCTCCCCAACTNTNTTAGAACCAATNTACTTAGTAGAAATTA
CTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTANAGGTATTG
TTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAG
TATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCC
CACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCA
CTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTG
ACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTTTGAGTTTTCAAAAATAGNA
ATCAAATCTAAAAAAAATTTTAAAAAAAAAA
Length of 3' end seq. 691
Connected seq. ID VHI223P
Connected seq. 
>VHI223P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATATGGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACT
GAAGAAGCTTATCTCTCTTT----------TCCAGAAGGTAACGGTGCCAATCTCCTCGT
CAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTT
CCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAA
TCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCC
AACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTNTNTTAGAACC
AATNTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACT
CAATCGTCGTANAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTC
TGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCA
CACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGT
CAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGC
CCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACT
TTGAGTTTTCAAAAATAGNAATCAAATCTAAAAAAAATTTTAAAAAAAAAA
Length of connected seq. 1241
Full length Seq ID -
Full length Seq. -
Length of full length seq. -