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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI220
Library VH
(Link to library)
Clone ID VHI220
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>VHI220 (VHI220Q) /CSM/VH/VHI2-A/VHI220Q.Seq.d/
XXXXXXXXXXCGNTGATTTACGTTNCCNCACTGCCGGTCAAGNTTTNCCNCAANGNGTTT
TNGATCATTGGGNTTCAATTGGNGTTGTCAACAAAAATAAAAAAGCCNCTGAAGTNGCTT
TAGCCNCCAAAAAAAAAAAANGTTTAGCCCCANAAATCCCANATNTTGACAAATTCCNCG
AAAAACTNTAAACNATTTAAGTCGCCCTTTGAGTTTTCAAAA
sequence update 2002. 9.10
Translated Amino Acid sequence 
---XDLRXXTAGQXXPQXVXDHWXSIGVVNKNKKAXEVALAXKKKKXLAPXIPXXDKFXE
KL*ti*val*vfk
Translated Amino Acid sequence (All Frames) 
Frame A:
---XDLRXXTAGQXXPQXVXDHWXSIGVVNKNKKAXEVALAXKKKKXLAPXIPXXDKFXE
KL*ti*val*vfk


Frame B:
---xiyvxxlpvkxxxxxfxiigxqlxlstkikkplkxl*ppkkkxv*pxksxxltnsxk
nxkxfkspfefsk


Frame C:
---*ftxphcrsxfxxxxfxslgfnwxcqqk*ksx*sxfsxqkkkxfspxnpxx*qiprk
txnxlsrplsfq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ383 (VHQ383Q) /CSM/VH/VHQ3-D/VHQ383Q.Seq.d/                        125   3e-28
VHO348 (VHO348Q) /CSM/VH/VHO3-B/VHO348Q.Seq.d/                        125   3e-28
VHI220 (VHI220Q) /CSM/VH/VHI2-A/VHI220Q.Seq.d/                        125   3e-28
VHG125 (VHG125Q) /CSM/VH/VHG1-B/VHG125Q.Seq.d/                        125   3e-28
VHD216 (VHD216Q) /CSM/VH/VHD2-A/VHD216Q.Seq.d/                        125   3e-28
VHC122 (VHC122Q) /CSM/VH/VHC1-A/VHC122Q.Seq.d/                        125   3e-28
VHP241 (VHP241Q) /CSM/VH/VHP2-B/VHP241Q.Seq.d/                        123   1e-27
VHN745 (VHN745Q) /CSM/VH/VHN7-B/VHN745Q.Seq.d/                        123   1e-27
VHO447 (VHO447Q) /CSM/VH/VHO4-B/VHO447Q.Seq.d/                        119   2e-26
VHK889 (VHK889Q) /CSM/VH/VHK8-D/VHK889Q.Seq.d/                        119   2e-26
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M26017|M26017.1 D.discoideum elongation factor 2 mRNA, complete cds.    90   2e-31  3
dna update 2003. 1. 9
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P28996) RecName: Full=Elongation factor 2;          Short=EF-2;...    32   7.0
AF213663_1(AF213663|pid:none) Euglena gracilis elongation factor...    32   9.2
protein update 2009. 3.11
PSORT 

 psg: 0.75  gvh: 0.35  alm: 0.58  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 40.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal

>> prediction for VHI220 is nuc
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID VHI220Z
3' end seq. 
>VHI220Z.Seq
NNNNNNNNNNCGNTGATTTACGTTNCCNCACTGCCGGTCAAGNTTTNCCNCAANGNGTTT
TNGATCATTGGGNTTCAATTGGNGTTGTCAACAAAAATAAAAAAGCCNCTGAAGTNGCTT
TAGCCNCCAAAAAAAAAAAANGTTTAGCCCCANAAATCCCANATNTTGACAAATTCCNCG
AAAAACTNTAAACNATTTAAGTCGCCCTTTGAGTTTTCAAAA
Length of 3' end seq. 222
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -