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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI204
Library VH
(Link to library)
Clone ID VHI204
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>VHI204 (VHI204Q) /CSM/VH/VHI2-A/VHI204Q.Seq.d/
GAAATGCCAAAGGAAGACAAATTACCAGCTGGTTGTACTTCACACGAATTTTTAATCAAT
CTTATCGATTCCCCAGGTCACGTTGATTTCAGTTCAGAAGTAACTGCTGCCCTCCGTGTT
ACTGATGGTGCTTTAGTCGTTATCGATTGCGTCGAAGGTGGTTTGCGTACXXXXXXXXXX
sequence update 2002.10.25
Translated Amino Acid sequence 
EMPKEDKLPAGCTSHEFLINLIDSPGHVDFSSEVTAALRVTDGALVVIDCVEGGLR---
Translated Amino Acid sequence (All Frames) 
Frame A:
EMPKEDKLPAGCTSHEFLINLIDSPGHVDFSSEVTAALRVTDGALVVIDCVEGGLR---


Frame B:
kcqrktnyqlvvlhtnf*silsipqvtlisvqk*llpsvllmvl*slsiaskvvcv---


Frame C:
nakgrqitswlyftrifnqsyrfprsr*fqfrsnccppcy*wcfsryrlrrrwfa---
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI204 (VHI204Q) /CSM/VH/VHI2-A/VHI204Q.Seq.d/                        337   3e-92
VHP874 (VHP874Q) /CSM/modify/h12/F/treated/VHP874Q.Seq.d/             321   2e-87
VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                        321   2e-87
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                        321   2e-87
VHP874 (VHP874Q) /CSM/modify/h12/F/treated/VHP874Q.Seq.d/             321   2e-87
VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                        321   2e-87
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                        321   2e-87
VHQ483 (VHQ483Q) /CSM/VH/VHQ4-D/VHQ483Q.Seq.d/                        321   2e-87
VHQ476 (VHQ476Q) /CSM/VH/VHQ4-D/VHQ476Q.Seq.d/                        321   2e-87
VHQ473 (VHQ473Q) /CSM/VH/VHQ4-D/VHQ473Q.Seq.d/                        321   2e-87
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ422796) Dictyostelium discoideum cDNA clone:ddv47g02, 5' ...   337   7e-89  1
(M26017) D.discoideum elongation factor 2 mRNA, complete cds.     317   7e-83  1
(AU061213) Dictyostelium discoideum slug cDNA, clone SLD549.      317   7e-83  1
(BJ427897) Dictyostelium discoideum cDNA clone:ddv63p23, 5' ...   317   7e-83  1
(BJ427892) Dictyostelium discoideum cDNA clone:ddv63o22, 5' ...   317   7e-83  1
(BJ427891) Dictyostelium discoideum cDNA clone:ddv63o21, 5' ...   317   7e-83  1
(BJ427889) Dictyostelium discoideum cDNA clone:ddv63o19, 5' ...   317   7e-83  1
(BJ427888) Dictyostelium discoideum cDNA clone:ddv63n23, 5' ...   317   7e-83  1
(BJ427882) Dictyostelium discoideum cDNA clone:ddv63m22, 5' ...   317   7e-83  1
(BJ427875) Dictyostelium discoideum cDNA clone:ddv63k24, 5' ...   317   7e-83  1
dna update 2008.11.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CP000881_15(CP000881|pid:none) Hemiselmis andersenii chromosome ...    84   2e-15
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...    84   2e-15
EU624179_1(EU624179|pid:none) Lomentaria orcadensis elongation f...    82   5e-15
EF033522_1(EF033522|pid:none) Hildenbrandia rubra voucher LLG133...    82   5e-15
D21259_1(D21259|pid:none) Entamoeba histolytica gene for elongat...    82   6e-15
(Q06193) RecName: Full=Elongation factor 2;          Short=EF-2;...    82   6e-15
EF033579_1(EF033579|pid:none) Sebdenia flabellata voucher GWS002...    81   1e-14
EF033564_1(EF033564|pid:none) Pachymenia carnosa voucher GWS0003...    80   2e-14
EF033529_1(EF033529|pid:none) Amphiroa fragilissima voucher LLG0...    80   2e-14
EF033551_1(EF033551|pid:none) Dilsea carnosa voucher GWS000746 e...    80   2e-14
protein update 2009. 7.13
PSORT 

 psg: 0.75  gvh: 0.38  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.14  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VHI204 is cyt
5' end seq. ID VHI204F
5' end seq. 
>VHI204F.Seq
GAAATGCCAAAGGAAGACAAATTACCAGCTGGTTGTACTTCACACGAATTTTTAATCAAT
CTTATCGATTCCCCAGGTCACGTTGATTTCAGTTCAGAAGTAACTGCTGCCCTCCGTGTT
ACTGATGGTGCTTTAGTCGTTATCGATTGCGTCGAAGGTGGTTTGCGTACNNNNNNNNNN
Length of 5' end seq. 180
3' end seq. ID -
3' end seq. -
Length of 3' end seq. -
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -