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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI150
Library VH
(Link to library)
Clone ID VHI150
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16439-1
Original site URL
Representative seq. ID VHI150P
(Link to Original site)
Representative DNA sequence 
>VHI150 (VHI150Q) /CSM/VH/VHI1-C/VHI150Q.Seq.d/
CTGGAAAAAATTTAATTTCATTTACAATTTGTTTATAATTACAAATAACTATTTTTATAT
TTTTATATTTATTATTTTTTTTAATATATTTTTTTATTTTCATTTTTTTGTATAAACTTA
CATAAAAAGATAAAAAGAAAAAAATGTCTAAACCAATGGATGTAGAACAAGAACCAAAAA
TTGATGATGCTTTATATAGCAGACAACTTTATGCATTATCTCATGAAACAATGAAAAAAA
TTACATCAACTTCAGTTTTAGTTGTAGGTTTACAAGGTCTTGGTATTGAAATTGTTAAAG
ATTTAAGTTTANCAGGTGTTAAATCTGTTACATTGTATGATAAAGAATTAGTTGAAATTA
AAGATTTGTCTTCTCAATTTTATTTTTCACCAGAACAAGTTGGTAAAGTTGGTAGAGCAG
ATGCATGTTTCCAAAAAGTTGTAGATTTAAATAATTATGTTAGAATTGATGTACATAATG
GAGAATTAAGTGATGAATTCTTAAAGAAATTCAATGTTGTAGTATTAGCCAATCAACCAT
TAGCATTACAACTCAAAGTGAATGAATTTTGTCATGCAAATAAAATTCATTTCATTAGTG
TTGAAACCAGAGGTGTTTTTGGTCAATTATTCAATGATTTTGGTGAACAATTTACAATXX
XXXXXXXXCAAGCTGCCAATGTTATTGTTCCAGATTTCACTCCAAAGAAGGTTAAGATTC
AAACNTCTGAAAATGAACCAGCTCCATCTTCAAACACTCAACAAGCTGGTGGTGATGCTG
AAGATGATCAATGTGATACCATTTTATCACAACTTCCACAACCAAGTGAAATGGCTGGTT
ACAAAATTAACTCTATTCAATTTGAAAAGGATGATGATACCAATCATCATATCGATTTCA
TTACCGCTACCTCAAATTTAAGAGCTACCAACTATGCAATCAGTCCAGCTGATAAACATA
AGACCAAAGGTATCGCCGGTAAAATCATTCCAGCTTTAGTTACCACTACTGCCGTAGTTG
CTGGTTTCGTTTGTATCGAATTGATTAAAGTTATTCAAAATAAAGCTTTGGAGAAATATA
AGAGCACCTTTATGAATTTAGGTATCCCATTCTTTGGTTTCGTTGAACCAATTGCCGCCC
CAAAGAACAAAATTCGTGAAGGCTGGACTTGGACACTTTGGGATCGTTTCGATGTTGATG
GTGATATCACTCTCAAGGAGTTTTTAGATCTCTTTGAAAAGAAACATGGTTTGGATATTT
CAATGCTCTCTTGTAAAGTTACACTTTTATATGCTCTCTTCACTGATAAGAAAACTAAAG
AGGAAAGATTAAAGATGAAAATCTCACAACTCTATGAAACCCTCTCAAAGAAACCATTAC
CAAAAGATAAGAAATATT
sequence update 2002.10.25
Translated Amino Acid sequence 
gknlisfticl*lqitififlyllffliyffififlyklt*KDKKKKMSKPMDVEQEPKI
DDALYSRQLYALSHETMKKITSTSVLVVGLQGLGIEIVKDLSLXGVKSVTLYDKELVEIK
DLSSQFYFSPEQVGKVGRADACFQKVVDLNNYVRIDVHNGELSDEFLKKFNVVVLANQPL
ALQLKVNEFCHANKIHFISVETRGVFGQLFNDFGEQFT---

---QAANVIVPDFTPKKVKIQTSENEPAPSSNTQQAGGDAEDDQCDTILSQLPQPSEMAG
YKINSIQFEKDDDTNHHIDFITATSNLRATNYAISPADKHKTKGIAGKIIPALVTTTAVV
AGFVCIELIKVIQNKALEKYKSTFMNLGIPFFGFVEPIAAPKNKIREGWTWTLWDRFDVD
GDITLKEFLDLFEKKHGLDISMLSCKVTLLYALFTDKKTKEERLKMKISQLYETLSKKPL
PKDKKY
Translated Amino Acid sequence (All Frames) 
Frame A:
leki*fhlqfvynyk*lflyfyiyyff*yiflfsffcinlhkkikrkkclnqwm*nknqk
lmmlyiadnfmhylmkq*kklhqlqf*l*vykvlvlkllki*vxqvlnllhcmikn*lkl
kicllnfifhqnklvklveqmhvskkl*i*iimlelmyimen*vmns*rnsml*y*pinh
*hynsk*mnfvmqikfislvlkpevflvnysmilvnnlq---

---QAANVIVPDFTPKKVKIQTSENEPAPSSNTQQAGGDAEDDQCDTILSQLPQPSEMAG
YKINSIQFEKDDDTNHHIDFITATSNLRATNYAISPADKHKTKGIAGKIIPALVTTTAVV
AGFVCIELIKVIQNKALEKYKSTFMNLGIPFFGFVEPIAAPKNKIREGWTWTLWDRFDVD
GDITLKEFLDLFEKKHGLDISMLSCKVTLLYALFTDKKTKEERLKMKISQLYETLSKKPL
PKDKKY

Frame B:
wkkfnfiynlfiitnnyfyififiiffniffyfhffv*tyikr*keknv*tngcrtrtkn
**cfi*qttlciis*nneknyinfsfscrftrswy*nc*rfkfxrc*icyiv**ris*n*
rfvfsilfftrtsw*sw*srcmfpkscrfk*lc*n*ct*wrik**ilkeiqccsisqsti
sittqse*ilsck*nsfh*c*nqrcfwsiiq*fw*tiyn---

---klpmllfqislqrrlrfkxlkmnqlhlqtlnklvvmlkminvipfyhnfhnqvkwlv
tkltlfnlkrmmipiiisislplpqi*elptmqsvqlinirpkvspvksfql*lpllp*l
lvsfvsn*lklfkiklwrnirapl*i*vshslvslnqlppqrtkfvkaglghfgivsmlm
vislsrsf*islkrnmvwifqcslvklhfymlsslirklkrkd*r*kshnsmkpsqrnhy
qkirni

Frame C:
gknlisfticl*lqitififlyllffliyffififlyklt*KDKKKKMSKPMDVEQEPKI
DDALYSRQLYALSHETMKKITSTSVLVVGLQGLGIEIVKDLSLXGVKSVTLYDKELVEIK
DLSSQFYFSPEQVGKVGRADACFQKVVDLNNYVRIDVHNGELSDEFLKKFNVVVLANQPL
ALQLKVNEFCHANKIHFISVETRGVFGQLFNDFGEQFT---

---scqcycsrfhskeg*dsnx*k*tssifkhstsww*c*r*sm*yhfittsttk*ngwl
qn*lysi*kg**yqssyrfhyrylkfksyqlcnqss**t*dqryrr*nhssfsyhycrsc
wfrlyrid*sysk*sfgei*ehlyefrypilwfr*tncrpkeqns*rldldtlgsfrc*w
*yhsqgvfrsl*ketwfgyfnall*sytficslh**en*rgkikdenlttl*nplketit
kr*ei
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI150 (VHI150Q) /CSM/VH/VHI1-C/VHI150Q.Seq.d/                       2373   0.0
VHB744 (VHB744Q) /CSM/VH/VHB7-B/VHB744Q.Seq.d/                       1441   0.0
VHA132 (VHA132Q) /CSM/VH/VHA1-B/VHA132Q.Seq.d/                       1441   0.0
SHD220 (SHD220Q) /CSM/SH/SHD2-A/SHD220Q.Seq.d/                       1441   0.0
CHQ706 (CHQ706Q) /CSM/CH/CHQ7-A/CHQ706Q.Seq.d/                       1441   0.0
AHQ886 (AHQ886Q) /CSM/AH/AHQ8-D/AHQ886Q.Seq.d/                       1441   0.0
AHH559 (AHH559Q) /CSM/AH/AHH5-C/AHH559Q.Seq.d/                       1441   0.0
AHN324 (AHN324Q) /CSM/AH/AHN3-A/AHN324Q.Seq.d/                       1439   0.0
VHA328 (VHA328Q) /CSM/VH/VHA3-B/VHA328Q.Seq.d/                       1437   0.0
CHS124 (CHS124Q) /CSM/CH/CHS1-A/CHS124Q.Seq.d/                       1433   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ441686) Dictyostelium discoideum cDNA clone:ddv47c13, 3' ...  1441   0.0    1
(BJ437464) Dictyostelium discoideum cDNA clone:ddv34h11, 3' ...  1441   0.0    1
(BJ436527) Dictyostelium discoideum cDNA clone:ddv31o07, 3' ...  1441   0.0    1
(BJ404932) Dictyostelium discoideum cDNA clone:dds30g06, 3' ...  1441   0.0    1
(BJ385494) Dictyostelium discoideum cDNA clone:ddc55l01, 3' ...  1441   0.0    1
(BJ357850) Dictyostelium discoideum cDNA clone:dda64l22, 3' ...  1441   0.0    1
(BJ352374) Dictyostelium discoideum cDNA clone:dda46e15, 3' ...  1441   0.0    1
(BJ355478) Dictyostelium discoideum cDNA clone:dda57p05, 3' ...  1439   0.0    1
(BJ436490) Dictyostelium discoideum cDNA clone:ddv31h07, 3' ...  1437   0.0    1
(BJ386426) Dictyostelium discoideum cDNA clone:ddc58o05, 3' ...  1433   0.0    1
dna update 2008.11.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55C16) RecName: Full=Ubiquitin-like modifier-activating enzyme...   491   e-137
EF468345_1(EF468345|pid:none) Pisum sativum ubiquitin activating...   257   6e-67
AE017341_129(AE017341|pid:none) Cryptococcus neoformans var. neo...   248   3e-64
BX908809_46(BX908809|pid:none) Neurospora crassa DNA linkage gro...   244   5e-63
CR382138_755(CR382138|pid:none) Debaryomyces hansenii strain CBS...   240   1e-61
AM270347_41(AM270347|pid:none) Aspergillus niger contig An15c020...   239   2e-61
AE016819_666(AE016819|pid:none) Ashbya gossypii (= Eremothecium ...   237   9e-61
CP000496_820(CP000496|pid:none) Pichia stipitis CBS 6054 chromos...   236   2e-60
(P22515) RecName: Full=Ubiquitin-activating enzyme E1 1;  &S3804...   235   3e-60
FN392321_874(FN392321|pid:none) Pichia pastoris GS115 chromosome...   235   3e-60
protein update 2009. 7.13
PSORT 

 psg: 0.50  gvh: 0.52  alm: 0.27  top: 0.43  tms: 0.07  mit: 0.21  mip: 0.00
 nuc: 0.13  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: nuclear
	 24.0 %: mitochondrial
	 16.0 %: cytoplasmic
	 12.0 %: endoplasmic reticulum
	  8.0 %: cytoskeletal
	  8.0 %: Golgi
	  4.0 %: vacuolar

>> prediction for VHI150 is nuc
5' end seq. ID VHI150F
5' end seq. 
>VHI150F.Seq
CTGGAAAAAATTTAATTTCATTTACAATTTGTTTATAATTACAAATAACTATTTTTATAT
TTTTATATTTATTATTTTTTTTAATATATTTTTTTATTTTCATTTTTTTGTATAAACTTA
CATAAAAAGATAAAAAGAAAAAAATGTCTAAACCAATGGATGTAGAACAAGAACCAAAAA
TTGATGATGCTTTATATAGCAGACAACTTTATGCATTATCTCATGAAACAATGAAAAAAA
TTACATCAACTTCAGTTTTAGTTGTAGGTTTACAAGGTCTTGGTATTGAAATTGTTAAAG
ATTTAAGTTTANCAGGTGTTAAATCTGTTACATTGTATGATAAAGAATTAGTTGAAATTA
AAGATTTGTCTTCTCAATTTTATTTTTCACCAGAACAAGTTGGTAAAGTTGGTAGAGCAG
ATGCATGTTTCCAAAAAGTTGTAGATTTAAATAATTATGTTAGAATTGATGTACATAATG
GAGAATTAAGTGATGAATTCTTAAAGAAATTCAATGTTGTAGTATTAGCCAATCAACCAT
TAGCATTACAACTCAAAGTGAATGAATTTTGTCATGCAAATAAAATTCATTTCATTAGTG
TTGAAACCAGAGGTGTTTTTGGTCAATTATTCAATGATTTTGGTGAACAATTTACAATNN
NNNNNNNN
Length of 5' end seq. 668
3' end seq. ID VHI150Z
3' end seq. 
>VHI150Z.Seq
NNNNNNNNNNCAAGCTGCCAATGTTATTGTTCCAGATTTCACTCCAAAGAAGGTTAAGAT
TCAAACNTCTGAAAATGAACCAGCTCCATCTTCAAACACTCAACAAGCTGGTGGTGATGC
TGAAGATGATCAATGTGATACCATTTTATCACAACTTCCACAACCAAGTGAAATGGCTGG
TTACAAAATTAACTCTATTCAATTTGAAAAGGATGATGATACCAATCATCATATCGATTT
CATTACCGCTACCTCAAATTTAAGAGCTACCAACTATGCAATCAGTCCAGCTGATAAACA
TAAGACCAAAGGTATCGCCGGTAAAATCATTCCAGCTTTAGTTACCACTACTGCCGTAGT
TGCTGGTTTCGTTTGTATCGAATTGATTAAAGTTATTCAAAATAAAGCTTTGGAGAAATA
TAAGAGCACCTTTATGAATTTAGGTATCCCATTCTTTGGTTTCGTTGAACCAATTGCCGC
CCCAAAGAACAAAATTCGTGAAGGCTGGACTTGGACACTTTGGGATCGTTTCGATGTTGA
TGGTGATATCACTCTCAAGGAGTTTTTAGATCTCTTTGAAAAGAAACATGGTTTGGATAT
TTCAATGCTCTCTTGTAAAGTTACACTTTTATATGCTCTCTTCACTGATAAGAAAACTAA
AGAGGAAAGATTAAAGATGAAAATCTCACAACTCTATGAAACCCTCTCAAAGAAACCATT
ACCAAAAGATAAGAAATATT
Length of 3' end seq. 740
Connected seq. ID VHI150P
Connected seq. 
>VHI150P.Seq
CTGGAAAAAATTTAATTTCATTTACAATTTGTTTATAATTACAAATAACTATTTTTATAT
TTTTATATTTATTATTTTTTTTAATATATTTTTTTATTTTCATTTTTTTGTATAAACTTA
CATAAAAAGATAAAAAGAAAAAAATGTCTAAACCAATGGATGTAGAACAAGAACCAAAAA
TTGATGATGCTTTATATAGCAGACAACTTTATGCATTATCTCATGAAACAATGAAAAAAA
TTACATCAACTTCAGTTTTAGTTGTAGGTTTACAAGGTCTTGGTATTGAAATTGTTAAAG
ATTTAAGTTTANCAGGTGTTAAATCTGTTACATTGTATGATAAAGAATTAGTTGAAATTA
AAGATTTGTCTTCTCAATTTTATTTTTCACCAGAACAAGTTGGTAAAGTTGGTAGAGCAG
ATGCATGTTTCCAAAAAGTTGTAGATTTAAATAATTATGTTAGAATTGATGTACATAATG
GAGAATTAAGTGATGAATTCTTAAAGAAATTCAATGTTGTAGTATTAGCCAATCAACCAT
TAGCATTACAACTCAAAGTGAATGAATTTTGTCATGCAAATAAAATTCATTTCATTAGTG
TTGAAACCAGAGGTGTTTTTGGTCAATTATTCAATGATTTTGGTGAACAATTTACAAT--
--------CAAGCTGCCAATGTTATTGTTCCAGATTTCACTCCAAAGAAGGTTAAGATTC
AAACNTCTGAAAATGAACCAGCTCCATCTTCAAACACTCAACAAGCTGGTGGTGATGCTG
AAGATGATCAATGTGATACCATTTTATCACAACTTCCACAACCAAGTGAAATGGCTGGTT
ACAAAATTAACTCTATTCAATTTGAAAAGGATGATGATACCAATCATCATATCGATTTCA
TTACCGCTACCTCAAATTTAAGAGCTACCAACTATGCAATCAGTCCAGCTGATAAACATA
AGACCAAAGGTATCGCCGGTAAAATCATTCCAGCTTTAGTTACCACTACTGCCGTAGTTG
CTGGTTTCGTTTGTATCGAATTGATTAAAGTTATTCAAAATAAAGCTTTGGAGAAATATA
AGAGCACCTTTATGAATTTAGGTATCCCATTCTTTGGTTTCGTTGAACCAATTGCCGCCC
CAAAGAACAAAATTCGTGAAGGCTGGACTTGGACACTTTGGGATCGTTTCGATGTTGATG
GTGATATCACTCTCAAGGAGTTTTTAGATCTCTTTGAAAAGAAACATGGTTTGGATATTT
CAATGCTCTCTTGTAAAGTTACACTTTTATATGCTCTCTTCACTGATAAGAAAACTAAAG
AGGAAAGATTAAAGATGAAAATCTCACAACTCTATGAAACCCTCTCAAAGAAACCATTAC
CAAAAGATAAGAAATATT
Length of connected seq. 1388
Full length Seq ID -
Full length Seq. -
Length of full length seq. -