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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI132
Library VH
(Link to library)
Clone ID VHI132
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHI132P
(Link to Original site)
Representative DNA sequence 
>VHI132 (VHI132Q) /CSM/VH/VHI1-B/VHI132Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATXXXXXXXXXXCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAA
AGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAA
CATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAG
AGGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTG
CCTCCCCAACTNTNTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTA
TTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTC
GTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTT
TCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATC
ATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCA
CCAGAAANAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAAC
TCTAAACAATTTAAGTCGCACTTTGAGTTTTCAAANATAGTAATCAAATCTAAAAAAAAT
TTTAAAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTED---

---VNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGWW
SNHPNCSSCTLCC*inclpnxxrtnllsrnycprkrywwyllctqss*rycyw*rtsyrf
piilcqssftsirilrfhr*ftfphcrssfptmclrslgfnwccqqr*esh*srfshqkx
krfsprnprs*qiprktlnnlsrtlsfqx**snlkkilkk
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlk---

---rqchqrcsife*n*rffrwcfpmghqrrccl**kherypfqsl*chpph*chpqrvv
vkssqllvvysmlln*lppqlx*nqst**kllpqktllvvftlysivvevlllvknvvsv
physlskliyqy*npsvspliyvptlpvklshnvssiiglqlvlstkirkplksl*ppex
ekv*pqksqiltnstknskqfkshfefsxivikskknfkk

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*h*r---

---VNVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGWW
SNHPNCSSCTLCC*inclpnxxrtnllsrnycprkrywwyllctqss*rycyw*rtsyrf
piilcqssftsirilrfhr*ftfphcrssfptmclrslgfnwccqqr*esh*srfshqkx
krfsprnprs*qiprktlnnlsrtlsfqx**snlkkilkk

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTED---

---smspkvfni*mklkilslvlsngppkkvlfvmkt*evsvsifmmspstlmpsteggg
qiiptarrvlyaaeltasptxlepiylveitapenaiggiysvlnrrrgivigeerrigs
plfsvkahlpvlesfgftadlrshtagqafpqcvfdhwasigvvnkdkkatevalatrxr
kglapeipdldkfhekl*ti*val*vfkxsnqi*kkf*kk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI132 (VHI132Q) /CSM/VH/VHI1-B/VHI132Q.Seq.d/                       2357   0.0
VHP749 (VHP749Q) /CSM/VH/VHP7-C/VHP749Q.Seq.d/                       1215   0.0
VHO803 (VHO803Q) /CSM/VH/VHO8-A/VHO803Q.Seq.d/                       1215   0.0
VHO787 (VHO787Q) /CSM/VH/VHO7-D/VHO787Q.Seq.d/                       1215   0.0
VHO504 (VHO504Q) /CSM/VH/VHO5-A/VHO504Q.Seq.d/                       1215   0.0
VHO104 (VHO104Q) /CSM/VH/VHO1-A/VHO104Q.Seq.d/                       1215   0.0
VHN502 (VHN502Q) /CSM/VH/VHN5-A/VHN502Q.Seq.d/                       1215   0.0
VHK310 (VHK310Q) /CSM/VH/VHK3-A/VHK310Q.Seq.d/                       1215   0.0
VHJ871 (VHJ871Q) /CSM/VH/VHJ8-C/VHJ871Q.Seq.d/                       1215   0.0
VHJ682 (VHJ682Q) /CSM/VH/VHJ6-D/VHJ682Q.Seq.d/                       1215   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ441665) Dictyostelium discoideum cDNA clone:ddv47o07, 3' ...  1231   0.0    1
(BJ427897) Dictyostelium discoideum cDNA clone:ddv63p23, 5' ...  1166   0.0    1
(BJ427892) Dictyostelium discoideum cDNA clone:ddv63o22, 5' ...  1166   0.0    1
(BJ427891) Dictyostelium discoideum cDNA clone:ddv63o21, 5' ...  1166   0.0    1
(BJ427888) Dictyostelium discoideum cDNA clone:ddv63n23, 5' ...  1166   0.0    1
(BJ427874) Dictyostelium discoideum cDNA clone:ddv63k23, 5' ...  1166   0.0    1
(BJ427873) Dictyostelium discoideum cDNA clone:ddv63k22, 5' ...  1166   0.0    1
(BJ427872) Dictyostelium discoideum cDNA clone:ddv63k21, 5' ...  1166   0.0    1
(BJ427858) Dictyostelium discoideum cDNA clone:ddv63h20, 5' ...  1166   0.0    1
(BJ427832) Dictyostelium discoideum cDNA clone:ddv63b20, 5' ...  1166   0.0    1
dna update 2008.11.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   264   6e-69
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   260   8e-68
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   259   1e-67
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   258   3e-67
AY026074_1(AY026074|pid:none) Monosiga brevicollis elongation fa...   257   7e-67
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   257   7e-67
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   256   2e-66
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   254   4e-66
AM494972_26(AM494972|pid:none) Leishmania braziliensis chromosom...   254   4e-66
CT005272_19(CT005272|pid:none) Leishmania major strain Friedlin,...   254   5e-66
protein update 2009. 7.13
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	 20.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VHI132 is cyt
5' end seq. ID VHI132F
5' end seq. 
>VHI132F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATNNNNNNNNNN
Length of 5' end seq. 618
3' end seq. ID VHI132Z
3' end seq. 
>VHI132Z.Seq
NNNNNNNNNNCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTT
TCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAG
GTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGGTGGT
GGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCA
ACTNTNTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGT
ATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGT
TCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCT
GATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCT
TCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAN
AGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACA
ATTTAAGTCGCACTTTGAGTTTTCAAANATAGTAATCAAATCTAAAAAAAATTTTAAAAA
AAA
Length of 3' end seq. 663
Connected seq. ID VHI132P
Connected seq. 
>VHI132P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGAT----------CGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAA
AGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAA
CATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAG
AGGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTG
CCTCCCCAACTNTNTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTA
TTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTC
GTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTT
TCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATC
ATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCA
CCAGAAANAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAAC
TCTAAACAATTTAAGTCGCACTTTGAGTTTTCAAANATAGTAATCAAATCTAAAAAAAAT
TTTAAAAAAAA
Length of connected seq. 1261
Full length Seq ID -
Full length Seq. -
Length of full length seq. -