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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI124
Library VH
(Link to library)
Clone ID VHI124
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15897-1
Original site URL
Representative seq. ID VHI124P
(Link to Original site)
Representative DNA sequence 
>VHI124 (VHI124Q) /CSM/VH/VHI1-A/VHI124Q.Seq.d/
ATNATTTCAATGAATATTTAATTAAATTCAATTTTATATATATATATAAAAAGATGAAAA
CTATTAAAAGTTTATTTTTACTGTCATTGTTGATAGTGAATTTATTAATAAGTTCAACAT
ATGGAAGTTCAATTAGAGTAAGCATTGTTGGAGGTGAAGAAGCTGAAGTCATTGAAAAAC
CACGTACTTTTGGAAATAAAAGAGAACTCAAATTAGAGTATAGTCAAATTTATCCAAAGA
AACAATTAAATCAAGAAAATATTAATTTCATGTCTGCCAGAGATACTTTTGTTGATAATT
TAATGTCAAAAATGTCAATCACTGAAAAAATTGGTCAAATGACTCAATTGGATATTACAA
CATTAACATCACCAAATACAATTACAATTAATGAAACAACATTAGCATATTATGCTAAAA
CTTATTATATTGGATCATATTTAAATTCACCAGTATCAGGTGGTTTAGCAGGTGACATTC
ATCATATTAATAGTAGTGTTTGGTTAGATATGATTAATACTATTCAAACCATTGTTXXXX
XXXXXXAGAAGCTGAAACTCCAGGGTGATATTTATGATCTCTCTATGGATCCAAATGAAG
TACTCTTATTACAACAATTGGTTGACACTGGTAAACCAGTAGTTTTAATTTTGGTTGAAG
CTCGTCCAAGAATTTTACCACCAGATCTCGTCTACAGTTGTGCTGCAGTTTTAATGGCTT
ATCTCCCAGGTAGTGAAGGTGGTAAACCAATCGCCAACATTCTCATGGGTAATGTAAATC
CATCTGGTCGTCTTCCTTTAACTTATCCAGGTACTACTGGTGATATCGGTGTTCCATATT
ACCACAAATACAGTGAAAATGGTGTCACCACTCCACTCTTCCAATTTGGTGATGGTCTTT
CTTATACCACTTTTAACTATACCAATCTCGCCTGTAGCAATTGTAAACCAATCTCTGGTC
AATCTGGTAACTACACTGGTGTTTTAGGTCAATCCTATACTTTCACTGTCACTGTCACAA
ACAATGGTAACGTTCAAGGTAAAGACTCTGTTCTCTTATATCTCTCTGATCTTTGGGCTC
AAGTTACTCCAGAAGTTAAAATGTTAAGAGGTTTCCAAAAAGTTGATCTTATGCCAGCTA
AATCTCAACAAATCTCTTTTACCCTCAATGCCTATGAATTCTCCTTTATTGGTGTCGATA
ATAAAATTACTTTAGAATCTGGTCAATTCATCATTAGGTGGTAATCAACAATTAGGT
sequence update 2002.10.25
Translated Amino Acid sequence 
XFNEYLIKFNFIYIYKKMKTIKSLFLLSLLIVNLLISSTYGSSIRVSIVGGEEAEVIEKP
RTFGNKRELKLEYSQIYPKKQLNQENINFMSARDTFVDNLMSKMSITEKIGQMTQLDITT
LTSPNTITINETTLAYYAKTYYIGSYLNSPVSGGLAGDIHHINSSVWLDMINTIQTIV--
-

---KLKLQGDIYDLSMDPNEVLLLQQLVDTGKPVVLILVEARPRILPPDLVYSCAAVLMA
YLPGSEGGKPIANILMGNVNPSGRLPLTYPGTTGDIGVPYYHKYSENGVTTPLFQFGDGL
SYTTFNYTNLACSNCKPISGQSGNYTGVLGQSYTFTVTVTNNGNVQGKDSVLLYLSDLWA
QVTPEVKMLRGFQKVDLMPAKSQQISFTLNAYEFSFIGVDNKITLESGQFIIRW*stir
Translated Amino Acid sequence (All Frames) 
Frame A:
xismni*lnsilyiyikr*kllkvyfychc***iy**vqhmevqle*allevkklkslkn
hvlleikensn*sivkfiqrnn*ikkilisclpeilllii*cqkcqslkklvk*lnwilq
h*hhqiqlqlmkqh*himlkliildhi*ihqyqvv*qvtfiilivvfg*i*lilfkpl--
-

---rs*nsrvifmislwiqmkysyynnwltlvnq*f*fwlklvqefyhqisstvvlqf*w
lisqvvkvvnqsptfswvm*ihlvvfl*liqvllvisvfhittntvkmvsplhssnlvmv
fliplltipispvaivnqslvnlvttlvf*vnpilslslsqtmvtfkvktlfsyislifg
lkllqklkc*evskklilcqlnlnksllpsmpmnspllvsiikll*nlvnsslggnqqlg

Frame B:
xfq*ifn*iqfyiyi*kdeny*kfiftvivdsefinkfniwkfn*skhcwr*rs*sh*kt
tyfwk*krtqirv*snlsketiksrky*fhvcqryfc**fnvknvnh*knwsndsigyyn
initkynyn**nnisilc*nllywiifkftsirwfsr*hssy***clvryd*yysnhc--
-

---eaetpg*yl*slygsk*stlittig*hw*tssfnfg*sssknfttrsrlqlccsfng
lspr**rw*tnrqhshg*cksiwsssfnlsryyw*yrcsilpqiq*kwchhstlpiw*ws
flyhf*lyqsrl*ql*tnlwsiw*lhwcfrsilyfhchchkqw*rsr*rlcslisl*slg
ssysrs*nvkrfpks*syas*istnlfypqcl*illywcr**nyfriwsihh*vvinn*

Frame C:
XFNEYLIKFNFIYIYKKMKTIKSLFLLSLLIVNLLISSTYGSSIRVSIVGGEEAEVIEKP
RTFGNKRELKLEYSQIYPKKQLNQENINFMSARDTFVDNLMSKMSITEKIGQMTQLDITT
LTSPNTITINETTLAYYAKTYYIGSYLNSPVSGGLAGDIHHINSSVWLDMINTIQTIV--
-

---KLKLQGDIYDLSMDPNEVLLLQQLVDTGKPVVLILVEARPRILPPDLVYSCAAVLMA
YLPGSEGGKPIANILMGNVNPSGRLPLTYPGTTGDIGVPYYHKYSENGVTTPLFQFGDGL
SYTTFNYTNLACSNCKPISGQSGNYTGVLGQSYTFTVTVTNNGNVQGKDSVLLYLSDLWA
QVTPEVKMLRGFQKVDLMPAKSQQISFTLNAYEFSFIGVDNKITLESGQFIIRW*stir
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI124 (VHI124Q) /CSM/VH/VHI1-A/VHI124Q.Seq.d/                       2337   0.0
VHH688 (VHH688Q) /CSM/VH/VHH6-D/VHH688Q.Seq.d/                       1380   0.0
VHC215 (VHC215Q) /CSM/VH/VHC2-A/VHC215Q.Seq.d/                       1376   0.0
VHA656 (VHA656Q) /CSM/VH/VHA6-C/VHA656Q.Seq.d/                       1376   0.0
VHA583 (VHA583Q) /CSM/VH/VHA5-D/VHA583Q.Seq.d/                       1374   0.0
VHB282 (VHB282Q) /CSM/VH/VHB2-D/VHB282Q.Seq.d/                       1372   0.0
CHL244 (CHL244Q) /CSM/CH/CHL2-B/CHL244Q.Seq.d/                       1372   0.0
VHQ110 (VHQ110Q) /CSM/VH/VHQ1-A/VHQ110Q.Seq.d/                       1370   0.0
CHI820 (CHI820Q) /CSM/CH/CHI8-A/CHI820Q.Seq.d/                       1366   0.0
VHN466 (VHN466Q) /CSM/VH/VHN4-C/VHN466Q.Seq.d/                       1362   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ441592) Dictyostelium discoideum cDNA clone:ddv47o05, 3' ...  1409   0.0    1
(BJ441518) Dictyostelium discoideum cDNA clone:ddv46o22, 3' ...  1362   0.0    2
(BJ437723) Dictyostelium discoideum cDNA clone:ddv35m04, 3' ...  1358   0.0    2
(BJ382310) Dictyostelium discoideum cDNA clone:ddc44g12, 3' ...  1344   0.0    3
(BJ445955) Dictyostelium discoideum cDNA clone:ddv61m06, 3' ...  1334   0.0    2
(BJ437941) Dictyostelium discoideum cDNA clone:ddv35i22, 3' ...  1334   0.0    2
(BJ436938) Dictyostelium discoideum cDNA clone:ddv32o14, 3' ...  1334   0.0    3
(BJ446533) Dictyostelium discoideum cDNA clone:ddv63c03, 3' ...  1330   0.0    3
(BJ438020) Dictyostelium discoideum cDNA clone:ddv36i03, 3' ...  1326   0.0    2
(BJ442826) Dictyostelium discoideum cDNA clone:ddv51h02, 3' ...  1314   0.0    2
dna update 2008.11.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

A49881(A49881)beta-glucosidase (EC 3.2.1.21) precursor, lysosoma...   428   e-118
CP000159_2777(CP000159|pid:none) Salinibacter ruber DSM 13855, c...   154   8e-36
CP000083_2301(CP000083|pid:none) Colwellia psychrerythraea 34H, ...   145   3e-33
CP000383_3519(CP000383|pid:none) Cytophaga hutchinsonii ATCC 334...   144   9e-33
CP000383_2243(CP000383|pid:none) Cytophaga hutchinsonii ATCC 334...   135   4e-30
CP000383_2239(CP000383|pid:none) Cytophaga hutchinsonii ATCC 334...   133   2e-29
CP000685_3497(CP000685|pid:none) Flavobacterium johnsoniae UW101...   128   4e-28
CP000388_3699(CP000388|pid:none) Pseudoalteromonas atlantica T6c...   123   1e-26
CP001146_680(CP001146|pid:none) Dictyoglomus thermophilum H-6-12...   122   2e-26
CP001032_813(CP001032|pid:none) Opitutus terrae PB90-1, complete...   121   5e-26
protein update 2009. 7.13
PSORT 

 psg: 0.74  gvh: 0.73  alm: 0.26  top: 0.07  tms: 0.07  mit: 0.34  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.79  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 20.0 %: cytoplasmic
	 20.0 %: nuclear
	 20.0 %: endoplasmic reticulum
	 16.0 %: Golgi
	 12.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for VHI124 is cyt
5' end seq. ID VHI124F
5' end seq. 
>VHI124F.Seq
ATNATTTCAATGAATATTTAATTAAATTCAATTTTATATATATATATAAAAAGATGAAAA
CTATTAAAAGTTTATTTTTACTGTCATTGTTGATAGTGAATTTATTAATAAGTTCAACAT
ATGGAAGTTCAATTAGAGTAAGCATTGTTGGAGGTGAAGAAGCTGAAGTCATTGAAAAAC
CACGTACTTTTGGAAATAAAAGAGAACTCAAATTAGAGTATAGTCAAATTTATCCAAAGA
AACAATTAAATCAAGAAAATATTAATTTCATGTCTGCCAGAGATACTTTTGTTGATAATT
TAATGTCAAAAATGTCAATCACTGAAAAAATTGGTCAAATGACTCAATTGGATATTACAA
CATTAACATCACCAAATACAATTACAATTAATGAAACAACATTAGCATATTATGCTAAAA
CTTATTATATTGGATCATATTTAAATTCACCAGTATCAGGTGGTTTAGCAGGTGACATTC
ATCATATTAATAGTAGTGTTTGGTTAGATATGATTAATACTATTCAAACCATTGTTNNNN
NNNNNN
Length of 5' end seq. 546
3' end seq. ID VHI124Z
3' end seq. 
>VHI124Z.Seq
NNNNNNNNNNAGAAGCTGAAACTCCAGGGTGATATTTATGATCTCTCTATGGATCCAAAT
GAAGTACTCTTATTACAACAATTGGTTGACACTGGTAAACCAGTAGTTTTAATTTTGGTT
GAAGCTCGTCCAAGAATTTTACCACCAGATCTCGTCTACAGTTGTGCTGCAGTTTTAATG
GCTTATCTCCCAGGTAGTGAAGGTGGTAAACCAATCGCCAACATTCTCATGGGTAATGTA
AATCCATCTGGTCGTCTTCCTTTAACTTATCCAGGTACTACTGGTGATATCGGTGTTCCA
TATTACCACAAATACAGTGAAAATGGTGTCACCACTCCACTCTTCCAATTTGGTGATGGT
CTTTCTTATACCACTTTTAACTATACCAATCTCGCCTGTAGCAATTGTAAACCAATCTCT
GGTCAATCTGGTAACTACACTGGTGTTTTAGGTCAATCCTATACTTTCACTGTCACTGTC
ACAAACAATGGTAACGTTCAAGGTAAAGACTCTGTTCTCTTATATCTCTCTGATCTTTGG
GCTCAAGTTACTCCAGAAGTTAAAATGTTAAGAGGTTTCCAAAAAGTTGATCTTATGCCA
GCTAAATCTCAACAAATCTCTTTTACCCTCAATGCCTATGAATTCTCCTTTATTGGTGTC
GATAATAAAATTACTTTAGAATCTGGTCAATTCATCATTAGGTGGTAATCAACAATTAGG
T
Length of 3' end seq. 721
Connected seq. ID VHI124P
Connected seq. 
>VHI124P.Seq
ATNATTTCAATGAATATTTAATTAAATTCAATTTTATATATATATATAAAAAGATGAAAA
CTATTAAAAGTTTATTTTTACTGTCATTGTTGATAGTGAATTTATTAATAAGTTCAACAT
ATGGAAGTTCAATTAGAGTAAGCATTGTTGGAGGTGAAGAAGCTGAAGTCATTGAAAAAC
CACGTACTTTTGGAAATAAAAGAGAACTCAAATTAGAGTATAGTCAAATTTATCCAAAGA
AACAATTAAATCAAGAAAATATTAATTTCATGTCTGCCAGAGATACTTTTGTTGATAATT
TAATGTCAAAAATGTCAATCACTGAAAAAATTGGTCAAATGACTCAATTGGATATTACAA
CATTAACATCACCAAATACAATTACAATTAATGAAACAACATTAGCATATTATGCTAAAA
CTTATTATATTGGATCATATTTAAATTCACCAGTATCAGGTGGTTTAGCAGGTGACATTC
ATCATATTAATAGTAGTGTTTGGTTAGATATGATTAATACTATTCAAACCATTGTT----
------AGAAGCTGAAACTCCAGGGTGATATTTATGATCTCTCTATGGATCCAAATGAAG
TACTCTTATTACAACAATTGGTTGACACTGGTAAACCAGTAGTTTTAATTTTGGTTGAAG
CTCGTCCAAGAATTTTACCACCAGATCTCGTCTACAGTTGTGCTGCAGTTTTAATGGCTT
ATCTCCCAGGTAGTGAAGGTGGTAAACCAATCGCCAACATTCTCATGGGTAATGTAAATC
CATCTGGTCGTCTTCCTTTAACTTATCCAGGTACTACTGGTGATATCGGTGTTCCATATT
ACCACAAATACAGTGAAAATGGTGTCACCACTCCACTCTTCCAATTTGGTGATGGTCTTT
CTTATACCACTTTTAACTATACCAATCTCGCCTGTAGCAATTGTAAACCAATCTCTGGTC
AATCTGGTAACTACACTGGTGTTTTAGGTCAATCCTATACTTTCACTGTCACTGTCACAA
ACAATGGTAACGTTCAAGGTAAAGACTCTGTTCTCTTATATCTCTCTGATCTTTGGGCTC
AAGTTACTCCAGAAGTTAAAATGTTAAGAGGTTTCCAAAAAGTTGATCTTATGCCAGCTA
AATCTCAACAAATCTCTTTTACCCTCAATGCCTATGAATTCTCCTTTATTGGTGTCGATA
ATAAAATTACTTTAGAATCTGGTCAATTCATCATTAGGTGGTAATCAACAATTAGGT
Length of connected seq. 1247
Full length Seq ID -
Full length Seq. -
Length of full length seq. -