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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI120
Library VH
(Link to library)
Clone ID VHI120
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12489-1
Original site URL
Representative seq. ID VHI120P
(Link to Original site)
Representative DNA sequence 
>VHI120 (VHI120Q) /CSM/VH/VHI1-A/VHI120Q.Seq.d/
ATACTATTTCGTGTATATATAAAAATAATAATGAAGTCATTATTTTTAAATTTTATATTT
TTATTAATTTTTTTGGGGAGAGTATTTTGTCAAACATGTCCAGCAGGAACAACATTAGCT
AATGGAGCATGTGTTAAACAAGTTATAACTCAAATTACTACAGCCACTTGTAGATCAGGT
TCAAAGAGTGATTTTTACTGGTTATCAGGTTCTGCAAATCCATGTCCATCCGGATGGTCA
GTGGAATACCCAGTCAGAACTGCACCAATTACAAATAATCAATTAACTTGGTATTGGTGT
GTAGACATTTTAGATGTTGGAGGCTTCACTACAGAAGNTCTTTTCTCATCAGTGCTAAAT
TGTGGTGGTTAGCAAGCCTATACAATTGGTACAATCACCTTCTCAACAACCAATAAATAT
TTGGTTGTTTCATTTTGTTCAAATCAAGGTGATATGGTTGAATCAAATTCTAAACCAATA
ACATCTGATGCTACTAAAAATTTATGTACAAAAGGAAAAGGAACAATCACAGATGTTAGG
TACCTAGAXXXXXXXXXXGTAATGATATAAATGAATGTTCAACTAATAATGNCGGCTGTA
GTCAAGTTTGTACAAATNCAGCAGGTAGTTTCTCATGTTCATGTAATTCAGGGTNATTTA
TTGAGTACTGATAAAAAGACTTGTACAGATAAAAATGAATGTTTAACTAATAATGGTGGT
TGTACACAAGTTTGTACAAATTCAGTTGGTAGTTTCTCATGTTCATGTAATTCAGGTTTT
ATATTAAATTCAAATAAATTATCATGTGATGATATAAATGAATGCGATCAAAAGATTTGT
GGAACTGCAAATTGCACTAATATACCAGGAAGTTATCAATGTTCATGTCCAAGTGGTTAT
TCATTTACTGGTTCAGGTTGTATTGATATAGATGAATGCGATCAAAAGATTTGTGGCACT
GCAAATTGTACAAATTCACCAGGAAGTTACCAATGTTCATGTCCAAGTGGTTACTCATTT
ACAGGTTCAAGTTGTATTGATATTGATGAATGTTCTGCTGATAATGGTGGTTGCAGTCAA
ATTTGTACAAATTCAGTTGGTAGTTTCTCATGTTCATGTAATTCAGGTTTTACATTAAAT
TCAAATAAATTATCATGTGATGATATAAATGAATGTGATCAAAATATTTGTGGAACTGCG
AATTGTGCAAACTCTCCAGGAAGTTATCAATGTTCATGTCCAAATGGTTACTCATTTACA
GGTTCAGGTTGTATCGATATTGATGAATGTTCTACTGATAATGGTGGTTGTAGTCAAATT
TGTACAAATTCAGTTGGTAGTTTCTCATGTTCATGNATTCAGGTTATACTTTAAATGTNA
TAATAAAAAGTCTTG
sequence update 2002.10.25
Translated Amino Acid sequence 
ilfrvyikiimkslflnfiflliflgrvfcqtcpagttlangacvkqvitqittatcrsg
sksdfywlsgsanpcpsgwsveypvrtapitnnqltwywcvdildvggfttexlfssvln
cgg*QAYTIGTITFSTTNKYLVVSFCSNQGDMVESNSKPITSDATKNLCTKGKGTITDVR
YL---

---NDINECSTNNXGCSQVCTNXAGSFSCSCNSGXFIEY**kdlyr*k*mfn**wwlyts
lykfsw*flmfm*frfyikfk*iim**yk*mrskdlwncklh*ytrklsmfmskwlfiyw
frly*yr*mrskdlwhcklykftrklpmfmskwlliyrfkly*y**mfc**wwlqsnlyk
fsw*flmfm*frfyikfk*iim**yk*m*skylwncelcklsrklsmfmskwlliyrfrl
yry**mfy**wwl*snlykfsw*flmfmxsgytlnviiksl
Translated Amino Acid sequence (All Frames) 
Frame A:
ilfrvyikiimkslflnfiflliflgrvfcqtcpagttlangacvkqvitqittatcrsg
sksdfywlsgsanpcpsgwsveypvrtapitnnqltwywcvdildvggfttexlfssvln
cgg*QAYTIGTITFSTTNKYLVVSFCSNQGDMVESNSKPITSDATKNLCTKGKGTITDVR
YL---

---vmi*mnvqlimxavvkfvqxqqvvshvhviqgxllstdkktctdknecltnnggctq
vctnsvgsfscscnsgfilnsnklscddinecdqkicgtanctnipgsyqcscpsgysft
gsgcididecdqkicgtanctnspgsyqcscpsgysftgsscididecsadnggcsqict
nsvgsfscscnsgftlnsnklscddinecdqnicgtancanspgsyqcscpngysftgsg
cididecstdnggcsqictnsvgsfscsxiqvil*mx**kvl

Frame B:
yyfvyi*k***shyf*ilyfy*ffwgeyfvkhvqqeqh*lmehvlnkl*lkllqplvdqv
qrviftgyqvlqihvhpdgqwntqselhqlqiin*lgigv*tf*mleaslqkxfshqc*i
vvvskpiqlvqspsqqpiniwlfhfvqikviwlnqilnq*hlmllkiyvqkekeqsqmlg
t*---

---**yk*mfn**xrl*sslykxsr*flmfm*frviy*vlikrlvqikmnv*limvvvhk
fvqiqlvvshvhviqvly*iqinyhvmi*mnaikrfvelqialiyqevinvhvqvvihll
vqvvli*mnaikrfvalqivqihqevtnvhvqvvthlqvqvvlilmnvllimvvavkfvq
iqlvvshvhviqvlh*iqinyhvmi*mnvikifvelrivqtlqevinvhvqmvthlqvqv
vsilmnvllimvvvvkfvqiqlvvshvhxfrlyfkcxnkks

Frame C:
tisciyknnneviifkfyifinffgesilsnmssrnnis*wsmc*tsynsnyyshl*irf
ke*fllvirfcksmsirmvsgipsqnctnyk*sinlvlvcrhfrcwrlhyrxsflisakl
wwlaslynwynhllnnq*ifgcfilfksr*yg*ikf*tnni*cy*kfmykrkrnnhrc*v
pr---

---NDINECSTNNXGCSQVCTNXAGSFSCSCNSGXFIEY**kdlyr*k*mfn**wwlyts
lykfsw*flmfm*frfyikfk*iim**yk*mrskdlwncklh*ytrklsmfmskwlfiyw
frly*yr*mrskdlwhcklykftrklpmfmskwlliyrfkly*y**mfc**wwlqsnlyk
fsw*flmfm*frfyikfk*iim**yk*m*skylwncelcklsrklsmfmskwlliyrfrl
yry**mfy**wwl*snlykfsw*flmfmxsgytlnviiksl
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI120 (VHI120Q) /CSM/VH/VHI1-A/VHI120Q.Seq.d/                       2524   0.0
VHM873 (VHM873Q) /CSM/VH/VHM8-D/VHM873Q.Seq.d/                       1459   0.0
VHO340 (VHO340Q) /CSM/VH/VHO3-B/VHO340Q.Seq.d/                         54   6e-06
VHH326 (VHH326Q) /CSM/VH/VHH3-B/VHH326Q.Seq.d/                         54   6e-06
SSI148 (SSI148Q) /CSM/SS/SSI1-B/SSI148Q.Seq.d/                         40   0.089
AFO729 (AFO729Q) /CSM/AF/AFO7-B/AFO729Q.Seq.d/                         40   0.089
VHA317 (VHA317Q) /CSM/VH/VHA3-A/VHA317Q.Seq.d/                         38   0.35
SSM107 (SSM107Q) /CSM/SS/SSM1-A/SSM107Q.Seq.d/                         38   0.35
VHL560 (VHL560Q) /CSM/VH/VHL5-C/VHL560Q.Seq.d/                         36   1.4
VHK341 (VHK341Q) /CSM/VH/VHK3-B/VHK341Q.Seq.d/                         36   1.4
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ441556) Dictyostelium discoideum cDNA clone:ddv47g05, 3' ...  1628   0.0    1
(BJ444553) Dictyostelium discoideum cDNA clone:ddv56b20, 3' ...  1263   0.0    4
(BJ422799) Dictyostelium discoideum cDNA clone:ddv47g05, 5' ...   934   0.0    2
(BJ425753) Dictyostelium discoideum cDNA clone:ddv56b20, 5' ...   170   1e-91  7
(BJ422228) Dictyostelium discoideum cDNA clone:ddv45d07, 5' ...    54   1e-10  5
(BJ426563) Dictyostelium discoideum cDNA clone:ddv59p09, 5' ...    54   4e-08  3
(FC651941) CAXW12205.fwd CAXW Lottia gigantea from female go...    46   4e-07  3
(AC116963) Dictyostelium discoideum chromosome 2 map 4657875...    54   4e-06  10
(FC651466) CAXW11941.fwd CAXW Lottia gigantea from female go...    46   2e-05  3
(CN220623) RJA067C11.ab1 RJbrain Gallus gallus cDNA 5', mRNA...    40   8e-05  4
dna update 2008.11.11
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC116963_25(AC116963|pid:none) Dictyostelium discoideum chromoso...   241   4e-62
AE014296_1363(AE014296|pid:none) Drosophila melanogaster chromos...   203   6e-52
BT032860_1(BT032860|pid:none) Drosophila melanogaster RE24628 fu...   203   6e-52
DQ900937_1(DQ900937|pid:none) Ciona intestinalis gamma-carboxygl...   206   1e-51
EU854565_1(EU854565|pid:none) Danio rerio fibrillin 4 (fbn4) mRN...   206   1e-51
AB177798_1(AB177798|pid:none) Homo sapiens FBN3 gene for fibrill...   201   4e-50
AB177799_1(AB177799|pid:none) Homo sapiens FBN3 gene for fibrill...   201   4e-50
AB384635_1(AB384635|pid:none) Synthetic construct DNA, clone: pF...   201   4e-50
(Q75N90) RecName: Full=Fibrillin-3; Flags: Precursor;  &AY165863...   201   4e-50
U03272_1(U03272|pid:none) Human fibrillin-2 mRNA, complete cds.       200   9e-50
protein update 2009. 7.13
PSORT 

 psg: 0.91  gvh: 0.32  alm: 0.48  top: 0.47  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 24.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for VHI120 is cyt
5' end seq. ID VHI120F
5' end seq. 
>VHI120F.Seq
ATACTATTTCGTGTATATATAAAAATAATAATGAAGTCATTATTTTTAAATTTTATATTT
TTATTAATTTTTTTGGGGAGAGTATTTTGTCAAACATGTCCAGCAGGAACAACATTAGCT
AATGGAGCATGTGTTAAACAAGTTATAACTCAAATTACTACAGCCACTTGTAGATCAGGT
TCAAAGAGTGATTTTTACTGGTTATCAGGTTCTGCAAATCCATGTCCATCCGGATGGTCA
GTGGAATACCCAGTCAGAACTGCACCAATTACAAATAATCAATTAACTTGGTATTGGTGT
GTAGACATTTTAGATGTTGGAGGCTTCACTACAGAAGNTCTTTTCTCATCAGTGCTAAAT
TGTGGTGGTTAGCAAGCCTATACAATTGGTACAATCACCTTCTCAACAACCAATAAATAT
TTGGTTGTTTCATTTTGTTCAAATCAAGGTGATATGGTTGAATCAAATTCTAAACCAATA
ACATCTGATGCTACTAAAAATTTATGTACAAAAGGAAAAGGAACAATCACAGATGTTAGG
TACCTAGANNNNNNNNNN
Length of 5' end seq. 558
3' end seq. ID VHI120Z
3' end seq. 
>VHI120Z.Seq
NNNNNNNNNNGTAATGATATAAATGAATGTTCAACTAATAATGNCGGCTGTAGTCAAGTT
TGTACAAATNCAGCAGGTAGTTTCTCATGTTCATGTAATTCAGGGTNATTTATTGAGTAC
TGATAAAAAGACTTGTACAGATAAAAATGAATGTTTAACTAATAATGGTGGTTGTACACA
AGTTTGTACAAATTCAGTTGGTAGTTTCTCATGTTCATGTAATTCAGGTTTTATATTAAA
TTCAAATAAATTATCATGTGATGATATAAATGAATGCGATCAAAAGATTTGTGGAACTGC
AAATTGCACTAATATACCAGGAAGTTATCAATGTTCATGTCCAAGTGGTTATTCATTTAC
TGGTTCAGGTTGTATTGATATAGATGAATGCGATCAAAAGATTTGTGGCACTGCAAATTG
TACAAATTCACCAGGAAGTTACCAATGTTCATGTCCAAGTGGTTACTCATTTACAGGTTC
AAGTTGTATTGATATTGATGAATGTTCTGCTGATAATGGTGGTTGCAGTCAAATTTGTAC
AAATTCAGTTGGTAGTTTCTCATGTTCATGTAATTCAGGTTTTACATTAAATTCAAATAA
ATTATCATGTGATGATATAAATGAATGTGATCAAAATATTTGTGGAACTGCGAATTGTGC
AAACTCTCCAGGAAGTTATCAATGTTCATGTCCAAATGGTTACTCATTTACAGGTTCAGG
TTGTATCGATATTGATGAATGTTCTACTGATAATGGTGGTTGTAGTCAAATTTGTACAAA
TTCAGTTGGTAGTTTCTCATGTTCATGNATTCAGGTTATACTTTAAATGTNATAATAAAA
AGTCTTG
Length of 3' end seq. 847
Connected seq. ID VHI120P
Connected seq. 
>VHI120P.Seq
ATACTATTTCGTGTATATATAAAAATAATAATGAAGTCATTATTTTTAAATTTTATATTT
TTATTAATTTTTTTGGGGAGAGTATTTTGTCAAACATGTCCAGCAGGAACAACATTAGCT
AATGGAGCATGTGTTAAACAAGTTATAACTCAAATTACTACAGCCACTTGTAGATCAGGT
TCAAAGAGTGATTTTTACTGGTTATCAGGTTCTGCAAATCCATGTCCATCCGGATGGTCA
GTGGAATACCCAGTCAGAACTGCACCAATTACAAATAATCAATTAACTTGGTATTGGTGT
GTAGACATTTTAGATGTTGGAGGCTTCACTACAGAAGNTCTTTTCTCATCAGTGCTAAAT
TGTGGTGGTTAGCAAGCCTATACAATTGGTACAATCACCTTCTCAACAACCAATAAATAT
TTGGTTGTTTCATTTTGTTCAAATCAAGGTGATATGGTTGAATCAAATTCTAAACCAATA
ACATCTGATGCTACTAAAAATTTATGTACAAAAGGAAAAGGAACAATCACAGATGTTAGG
TACCTAGA----------GTAATGATATAAATGAATGTTCAACTAATAATGNCGGCTGTA
GTCAAGTTTGTACAAATNCAGCAGGTAGTTTCTCATGTTCATGTAATTCAGGGTNATTTA
TTGAGTACTGATAAAAAGACTTGTACAGATAAAAATGAATGTTTAACTAATAATGGTGGT
TGTACACAAGTTTGTACAAATTCAGTTGGTAGTTTCTCATGTTCATGTAATTCAGGTTTT
ATATTAAATTCAAATAAATTATCATGTGATGATATAAATGAATGCGATCAAAAGATTTGT
GGAACTGCAAATTGCACTAATATACCAGGAAGTTATCAATGTTCATGTCCAAGTGGTTAT
TCATTTACTGGTTCAGGTTGTATTGATATAGATGAATGCGATCAAAAGATTTGTGGCACT
GCAAATTGTACAAATTCACCAGGAAGTTACCAATGTTCATGTCCAAGTGGTTACTCATTT
ACAGGTTCAAGTTGTATTGATATTGATGAATGTTCTGCTGATAATGGTGGTTGCAGTCAA
ATTTGTACAAATTCAGTTGGTAGTTTCTCATGTTCATGTAATTCAGGTTTTACATTAAAT
TCAAATAAATTATCATGTGATGATATAAATGAATGTGATCAAAATATTTGTGGAACTGCG
AATTGTGCAAACTCTCCAGGAAGTTATCAATGTTCATGTCCAAATGGTTACTCATTTACA
GGTTCAGGTTGTATCGATATTGATGAATGTTCTACTGATAATGGTGGTTGTAGTCAAATT
TGTACAAATTCAGTTGGTAGTTTCTCATGTTCATGNATTCAGGTTATACTTTAAATGTNA
TAATAAAAAGTCTTG
Length of connected seq. 1385
Full length Seq ID -
Full length Seq. -
Length of full length seq. -