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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHH726
Library VH
(Link to library)
Clone ID VHH726
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11527-1
Original site URL
Representative seq. ID VHH726P
(Link to Original site)
Representative DNA sequence 
>VHH726 (VHH726Q) /CSM/VH/VHH7-B/VHH726Q.Seq.d/
AAATTGGGCAAGACTTGACCATTGTAAAAAAGGATGTGAATTAAAAAATGATTTTGAAGA
TGTTAAACCAACAACATTAACAGAGAGAGGAGCATTATTTGAATCAGCAAGATGTTTAAA
ATGTGCAGATGCACCATGTCAAAAAGGATGTCCAACTCAATTGGATATTAAATCATTTAT
TAGTTCAATTTCAACCAAAAACTATTATGGAGCAGCAAAGACAATTTTCTCTGATAATCC
ATTAGGTTTAACATGTGGTATGGTTTGTCCAGTTAGTTCATTATGTCAATATGGTTGTAA
TTTAGCAGCAACAGAAGAGGGACCAATCAATATTGGTGGTCTTCAACAATTTGCCACTGA
AGTATTCAAAAAGATGAATATTCCACAAATTCGTGATCCATCCTTAACACCATTGAGTCA
ATTACCAGAGAGTTATAAAGCAAAGATTGCTTTGATTGGTTGTGGTCCAACCTCAATTAG
TTGTGCAACATTTTTAGGTCGTTTAGGTTACACAGATGTCACCATCTTTGAAAAGGAACA
ATACTTGGGTGGTCTTAGCAGTAGTGAAATTCCAAACTATCGTCTCAATTATGAAGTGGT
TGAATTTGAAAXXXXXXXXXXTGTTTCAGGTAATGCTACTCGTCCAATTGCTTTACGTGC
AGTTTCTTCCATTAGAAAATCTTTACCAGATTATCCAATTATGGCAACTGGTGGTGCTGA
TTCTGCTGATGCCACTATTCAATTCCTCCATTGTGGTGCTTCAGTAGTTCAAATTTGTTC
ATCCGTTCAAAATCAAGATTTCACCGTCGTTCAAGATTATATCACTGGTCTTAAGACTTA
TCTTTACATGCAATCTCGTGAAGATTTATTGCAATGGGATGGTCAATCACCACCAAATGA
AATCATAAACAAAAATCCAAAATTAAAAGGTTTACCAAAATTCGGTAAATATCTTTTAGA
ACGTAATAGAATCGATACTGAAGAGAAACAAAACATTGATTTACAAAAAGTTAAGAATCC
ATTAGTACCATCACCAAATCCAACTCATCCAGTTCCATCTCTCAAAGATCAAATTAATCG
TGCAATCCCAAGAATTGGTCGTCATGATGATCTTAAACGTGATCAACAAGTAGTTGCTCT
TATCGATGAAGATAAATGTATCAATTGTGGTAAATGTTATATGACTTGTAATGATTCTGG
TTATCAAGCTATTAAATTCGATGGTAAAACTCATATTCCATTAGTCACTGATTTATGTAC
TGGTTGTGATTTATGTTTATCAGTTTGTCCAGTACCAGATTGCATTACAATGGTACCACG
TGAAACAATTTATGTACCAG
sequence update 2002.10.25
Translated Amino Acid sequence 
NWARLDHCKKGCELKNDFEDVKPTTLTERGALFESARCLKCADAPCQKGCPTQLDIKSFI
SSISTKNYYGAAKTIFSDNPLGLTCGMVCPVSSLCQYGCNLAATEEGPINIGGLQQFATE
VFKKMNIPQIRDPSLTPLSQLPESYKAKIALIGCGPTSISCATFLGRLGYTDVTIFEKEQ
YLGGLSSSEIPNYRLNYEVVEFE---

---VSGNATRPIALRAVSSIRKSLPDYPIMATGGADSADATIQFLHCGASVVQICSSVQN
QDFTVVQDYITGLKTYLYMQSREDLLQWDGQSPPNEIINKNPKLKGLPKFGKYLLERNRI
DTEEKQNIDLQKVKNPLVPSPNPTHPVPSLKDQINRAIPRIGRHDDLKRDQQVVALIDED
KCINCGKCYMTCNDSGYQAIKFDGKTHIPLVTDLCTGCDLCLSVCPVPDCITMVPRETIY
VP
Translated Amino Acid sequence (All Frames) 
Frame A:
klgkt*pl*krm*ikk*f*rc*tnninrersii*iskmfkmcrctmskrmsnsigy*iiy
*fnfnqkllwsskdnfl**sirfnmwyglss*fimsiwl*fssnrrgtnqywwsstich*
siqkdeystns*silntiesitrel*skdcfdwlwsnln*lcnifrsfrlhrchhl*kgt
ilgws*q**nsklssql*sg*i*---

---cfr*cyssncftcsffh*kiftrlsnygnwwc*fc*chysipplwcfsssnlfirsk
srfhrrsrlyhws*dlslhais*rfiamgwsittk*nhkqkskikrftkir*isfrt**n
ry*retkh*ftks*esistitksnssssisqrsn*scnpknwss**s*t*stsscsyr*r
*myqlw*mlydl**fwlssy*irw*nsysish*fmywl*fmfislsstrlhyngtt*nnl
ct

Frame B:
NWARLDHCKKGCELKNDFEDVKPTTLTERGALFESARCLKCADAPCQKGCPTQLDIKSFI
SSISTKNYYGAAKTIFSDNPLGLTCGMVCPVSSLCQYGCNLAATEEGPINIGGLQQFATE
VFKKMNIPQIRDPSLTPLSQLPESYKAKIALIGCGPTSISCATFLGRLGYTDVTIFEKEQ
YLGGLSSSEIPNYRLNYEVVEFE---

---VSGNATRPIALRAVSSIRKSLPDYPIMATGGADSADATIQFLHCGASVVQICSSVQN
QDFTVVQDYITGLKTYLYMQSREDLLQWDGQSPPNEIINKNPKLKGLPKFGKYLLERNRI
DTEEKQNIDLQKVKNPLVPSPNPTHPVPSLKDQINRAIPRIGRHDDLKRDQQVVALIDED
KCINCGKCYMTCNDSGYQAIKFDGKTHIPLVTDLCTGCDLCLSVCPVPDCITMVPRETIY
VP

Frame C:
igqdltivkkdvn*kmilkmlnqqh*qreehylnqqdv*nvqmhhvkkdvqlnwilnhll
vqfqpktimeqqrqfsliih*v*hvvwfvqlvhyvnmvvi*qqqkrdqsilvvfnnlplk
yskr*ifhkfvihp*hh*vnyqrvikqrll*lvvvqpqlvvqhf*vv*vtqmspslkrnn
twvvlavvkfqtivsimkwlnlk---

---fqvmllvqllyvqflplenlyqiiqlwqlvvlillmplfnssivvlq*fkfvhpfki
kispsfkiislvlrliftcnlvkiycngmvnhhqmks*tkiqn*kvyqnsvnif*nvies
ilkrnktliykklrih*yhhqiqliqfhlskiklivqsqelvvmmilnvink*lllsmki
nvsivvnvi*lvmilvikllnsmvklifh*sliyvlvviyvyqfvqyqialqwyhvkqfm
yq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHH726 (VHH726Q) /CSM/VH/VHH7-B/VHH726Q.Seq.d/                       2597   0.0
CHR689 (CHR689Q) /CSM/CH/CHR6-D/CHR689Q.Seq.d/                       1425   0.0
CHI778 (CHI778Q) /CSM/CH/CHI7-D/CHI778Q.Seq.d/                       1425   0.0
SHD855 (SHD855Q) /CSM/SH/SHD8-C/SHD855Q.Seq.d/                       1419   0.0
CHL263 (CHL263Q) /CSM/CH/CHL2-C/CHL263Q.Seq.d/                       1419   0.0
SHK871 (SHK871Q) /CSM/SH/SHK8-C/SHK871Q.Seq.d/                       1417   0.0
SHG712 (SHG712Q) /CSM/SH/SHG7-A/SHG712Q.Seq.d/                       1417   0.0
VHO413 (VHO413Q) /CSM/VH/VHO4-A/VHO413Q.Seq.d/                       1409   0.0
VHI412 (VHI412Q) /CSM/VH/VHI4-A/VHI412Q.Seq.d/                       1409   0.0
SHJ895 (SHJ895Q) /CSM/SH/SHJ8-D/SHJ895Q.Seq.d/                       1409   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AF545064) Dictyostelium discoideum dihydropyrimidine dehydr...  1425   0.0    1
(BJ441294) Dictyostelium discoideum cDNA clone:ddv46d07, 3' ...  1425   0.0    1
(BJ386290) Dictyostelium discoideum cDNA clone:ddc57a24, 3' ...  1425   0.0    1
(BJ380965) Dictyostelium discoideum cDNA clone:ddc39l19, 3' ...  1425   0.0    1
(BJ405441) Dictyostelium discoideum cDNA clone:dds31n14, 3' ...  1419   0.0    1
(BJ382418) Dictyostelium discoideum cDNA clone:ddc44m16, 3' ...  1419   0.0    1
(BJ408397) Dictyostelium discoideum cDNA clone:dds45n18, 3' ...  1417   0.0    1
(BJ406986) Dictyostelium discoideum cDNA clone:dds37h03, 3' ...  1417   0.0    1
(BJ445320) Dictyostelium discoideum cDNA clone:ddv59j04, 3' ...  1386   0.0    2
(BJ441559) Dictyostelium discoideum cDNA clone:ddv47h04, 3' ...  1386   0.0    2
dna update 2008.11.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55FT1) RecName: Full=Dihydropyrimidine dehydrogenase [NADP+]; ...   497   e-139
(Q12882) RecName: Full=Dihydropyrimidine dehydrogenase [NADP+]; ...   302   2e-80
A54718(A54718) dihydropyrimidine dehydrogenase (NADP) (EC 1.3.1....   302   2e-80
AK291217_1(AK291217|pid:none) Homo sapiens cDNA FLJ76825 complet...   302   2e-80
BC131778_1(BC131778|pid:none) Homo sapiens dihydropyrimidine deh...   302   2e-80
AL356457_2(AL356457|pid:none) Human DNA sequence from clone RP11...   302   2e-80
(Q8CHR6) RecName: Full=Dihydropyrimidine dehydrogenase [NADP+]; ...   301   4e-80
BC080003_1(BC080003|pid:none) Xenopus laevis MGC81821 protein, m...   299   1e-79
(Q5R895) RecName: Full=Dihydropyrimidine dehydrogenase [NADP+]; ...   298   4e-79
EU850439_1(EU850439|pid:none) Adineta vaga dihydrogenpyrimidine ...   291   4e-77
protein update 2009. 7.13
PSORT 

 psg: 0.53  gvh: 0.43  alm: 0.35  top: 0.53  tms: 0.00  mit: 0.18  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 68.0 %: cytoplasmic
	 20.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VHH726 is cyt
5' end seq. ID VHH726F
5' end seq. 
>VHH726F.Seq
AAATTGGGCAAGACTTGACCATTGTAAAAAAGGATGTGAATTAAAAAATGATTTTGAAGA
TGTTAAACCAACAACATTAACAGAGAGAGGAGCATTATTTGAATCAGCAAGATGTTTAAA
ATGTGCAGATGCACCATGTCAAAAAGGATGTCCAACTCAATTGGATATTAAATCATTTAT
TAGTTCAATTTCAACCAAAAACTATTATGGAGCAGCAAAGACAATTTTCTCTGATAATCC
ATTAGGTTTAACATGTGGTATGGTTTGTCCAGTTAGTTCATTATGTCAATATGGTTGTAA
TTTAGCAGCAACAGAAGAGGGACCAATCAATATTGGTGGTCTTCAACAATTTGCCACTGA
AGTATTCAAAAAGATGAATATTCCACAAATTCGTGATCCATCCTTAACACCATTGAGTCA
ATTACCAGAGAGTTATAAAGCAAAGATTGCTTTGATTGGTTGTGGTCCAACCTCAATTAG
TTGTGCAACATTTTTAGGTCGTTTAGGTTACACAGATGTCACCATCTTTGAAAAGGAACA
ATACTTGGGTGGTCTTAGCAGTAGTGAAATTCCAAACTATCGTCTCAATTATGAAGTGGT
TGAATTTGAAANNNNNNNNNN
Length of 5' end seq. 621
3' end seq. ID VHH726Z
3' end seq. 
>VHH726Z.Seq
NNNNNNNNNNTGTTTCAGGTAATGCTACTCGTCCAATTGCTTTACGTGCAGTTTCTTCCA
TTAGAAAATCTTTACCAGATTATCCAATTATGGCAACTGGTGGTGCTGATTCTGCTGATG
CCACTATTCAATTCCTCCATTGTGGTGCTTCAGTAGTTCAAATTTGTTCATCCGTTCAAA
ATCAAGATTTCACCGTCGTTCAAGATTATATCACTGGTCTTAAGACTTATCTTTACATGC
AATCTCGTGAAGATTTATTGCAATGGGATGGTCAATCACCACCAAATGAAATCATAAACA
AAAATCCAAAATTAAAAGGTTTACCAAAATTCGGTAAATATCTTTTAGAACGTAATAGAA
TCGATACTGAAGAGAAACAAAACATTGATTTACAAAAAGTTAAGAATCCATTAGTACCAT
CACCAAATCCAACTCATCCAGTTCCATCTCTCAAAGATCAAATTAATCGTGCAATCCCAA
GAATTGGTCGTCATGATGATCTTAAACGTGATCAACAAGTAGTTGCTCTTATCGATGAAG
ATAAATGTATCAATTGTGGTAAATGTTATATGACTTGTAATGATTCTGGTTATCAAGCTA
TTAAATTCGATGGTAAAACTCATATTCCATTAGTCACTGATTTATGTACTGGTTGTGATT
TATGTTTATCAGTTTGTCCAGTACCAGATTGCATTACAATGGTACCACGTGAAACAATTT
ATGTACCAG
Length of 3' end seq. 729
Connected seq. ID VHH726P
Connected seq. 
>VHH726P.Seq
AAATTGGGCAAGACTTGACCATTGTAAAAAAGGATGTGAATTAAAAAATGATTTTGAAGA
TGTTAAACCAACAACATTAACAGAGAGAGGAGCATTATTTGAATCAGCAAGATGTTTAAA
ATGTGCAGATGCACCATGTCAAAAAGGATGTCCAACTCAATTGGATATTAAATCATTTAT
TAGTTCAATTTCAACCAAAAACTATTATGGAGCAGCAAAGACAATTTTCTCTGATAATCC
ATTAGGTTTAACATGTGGTATGGTTTGTCCAGTTAGTTCATTATGTCAATATGGTTGTAA
TTTAGCAGCAACAGAAGAGGGACCAATCAATATTGGTGGTCTTCAACAATTTGCCACTGA
AGTATTCAAAAAGATGAATATTCCACAAATTCGTGATCCATCCTTAACACCATTGAGTCA
ATTACCAGAGAGTTATAAAGCAAAGATTGCTTTGATTGGTTGTGGTCCAACCTCAATTAG
TTGTGCAACATTTTTAGGTCGTTTAGGTTACACAGATGTCACCATCTTTGAAAAGGAACA
ATACTTGGGTGGTCTTAGCAGTAGTGAAATTCCAAACTATCGTCTCAATTATGAAGTGGT
TGAATTTGAAA----------TGTTTCAGGTAATGCTACTCGTCCAATTGCTTTACGTGC
AGTTTCTTCCATTAGAAAATCTTTACCAGATTATCCAATTATGGCAACTGGTGGTGCTGA
TTCTGCTGATGCCACTATTCAATTCCTCCATTGTGGTGCTTCAGTAGTTCAAATTTGTTC
ATCCGTTCAAAATCAAGATTTCACCGTCGTTCAAGATTATATCACTGGTCTTAAGACTTA
TCTTTACATGCAATCTCGTGAAGATTTATTGCAATGGGATGGTCAATCACCACCAAATGA
AATCATAAACAAAAATCCAAAATTAAAAGGTTTACCAAAATTCGGTAAATATCTTTTAGA
ACGTAATAGAATCGATACTGAAGAGAAACAAAACATTGATTTACAAAAAGTTAAGAATCC
ATTAGTACCATCACCAAATCCAACTCATCCAGTTCCATCTCTCAAAGATCAAATTAATCG
TGCAATCCCAAGAATTGGTCGTCATGATGATCTTAAACGTGATCAACAAGTAGTTGCTCT
TATCGATGAAGATAAATGTATCAATTGTGGTAAATGTTATATGACTTGTAATGATTCTGG
TTATCAAGCTATTAAATTCGATGGTAAAACTCATATTCCATTAGTCACTGATTTATGTAC
TGGTTGTGATTTATGTTTATCAGTTTGTCCAGTACCAGATTGCATTACAATGGTACCACG
TGAAACAATTTATGTACCAG
Length of connected seq. 1330
Full length Seq ID -
Full length Seq. -
Length of full length seq. -