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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHH687
Library VH
(Link to library)
Clone ID VHH687
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VHH687P
(Link to Original site)
Representative DNA sequence 
>VHH687 (VHH687Q) /CSM/VH/VHH6-D/VHH687Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAXXXXXXXXXXATCCAAAGA
TGATCCAAAAGCTCGTGCTAACTATCTCGCTGATAACCACGAATGGGATAAAAATGATGC
CATGAACATTTGGTCTTTCGGTCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCAC
CAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGC
CACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGA
TGTCACCCTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCG
TCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTT
AGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCG
TAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGC
TCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGG
TCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGA
TAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAAT
CCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTNAGTTTTCA
AA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLL---

---SKDDPKARANYLADNHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSFV
GAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPTL
LEPIYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTADL
RSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti*
valsfq
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy---

---iqr*skssc*lsr**prmg*k*chehlvfrsrr*rcqsprqchqrcsife*n*rffr
wcfpmghqrrccl**kherypfqsl*chpph*chpqrwwsnhpncssctlcc*inclpns
lrtnllsrnycprkrywwyllctqss*rycyw*rtsyrfpiilcqssftsirilrfhr*f
tfphcrssfptmclrslgfnwccqqr*esh*srfshqkekrfsprnprs*qiprktlnnl
srtxfs

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsfli---

---SKDDPKARANYLADNHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSFV
GAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPTL
LEPIYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTADL
RSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti*
valsfq

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLL---

---pkmiqklvltislittngikmmp*tfglsvqkvtvpisssmspkvfni*mklkilsl
vlsngppkkvlfvmkt*evsvsifmmspstlmpstevvvkssqllvvysmlln*lppqls
*nqst**kllpqktllvvftlysivvevlllvknvvsvphyslskliyqy*npsvspliy
vptlpvklshnvssiiglqlvlstkirkplksl*pperekv*pqksqiltnstknskqfk
shxvfk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHH687 (VHH687Q) /CSM/VH/VHH6-D/VHH687Q.Seq.d/                       2397   0.0
VHO711 (VHO711Q) /CSM/VH/VHO7-A/VHO711Q.Seq.d/                       1443   0.0
VHK672 (VHK672Q) /CSM/VH/VHK6-C/VHK672Q.Seq.d/                       1443   0.0
VHK338 (VHK338Q) /CSM/VH/VHK3-B/VHK338Q.Seq.d/                       1443   0.0
VHJ680 (VHJ680Q) /CSM/VH/VHJ6-D/VHJ680Q.Seq.d/                       1443   0.0
VHJ671 (VHJ671Q) /CSM/VH/VHJ6-C/VHJ671Q.Seq.d/                       1443   0.0
VHJ541 (VHJ541Q) /CSM/VH/VHJ5-B/VHJ541Q.Seq.d/                       1443   0.0
VHI270 (VHI270Q) /CSM/VH/VHI2-C/VHI270Q.Seq.d/                       1443   0.0
VHH733 (VHH733Q) /CSM/VH/VHH7-B/VHH733Q.Seq.d/                       1443   0.0
VHH502 (VHH502Q) /CSM/VH/VHH5-A/VHH502Q.Seq.d/                       1443   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ445614) Dictyostelium discoideum cDNA clone:ddv60f03, 3' ...  1443   0.0    1
(BJ443368) Dictyostelium discoideum cDNA clone:ddv52o18, 3' ...  1443   0.0    1
(BJ442939) Dictyostelium discoideum cDNA clone:ddv51l09, 3' ...  1443   0.0    1
(BJ442780) Dictyostelium discoideum cDNA clone:ddv50o20, 3' ...  1443   0.0    1
(BJ442687) Dictyostelium discoideum cDNA clone:ddv50m18, 3' ...  1443   0.0    1
(BJ442548) Dictyostelium discoideum cDNA clone:ddv50a11, 3' ...  1443   0.0    1
(BJ441725) Dictyostelium discoideum cDNA clone:ddv47k18, 3' ...  1443   0.0    1
(BJ441507) Dictyostelium discoideum cDNA clone:ddv46m22, 3' ...  1443   0.0    1
(BJ441285) Dictyostelium discoideum cDNA clone:ddv46b09, 3' ...  1443   0.0    1
(BJ441209) Dictyostelium discoideum cDNA clone:ddv46c01, 3' ...  1443   0.0    1
dna update 2008.11.10
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   301   3e-80
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   300   7e-80
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   296   8e-79
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   295   2e-78
BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   295   2e-78
BT059038_1(BT059038|pid:none) Salmo salar clone ssal-rgf-521-328...   295   2e-78
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   295   2e-78
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   295   2e-78
AK032997_1(AK032997|pid:none) Mus musculus 12 days embryo male w...   295   2e-78
(P05197) RecName: Full=Elongation factor 2;          Short=EF-2;...   295   2e-78
protein update 2009. 7.13
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHH687 is cyt
5' end seq. ID VHH687F
5' end seq. 
>VHH687F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTANNNNNNNNNN
Length of 5' end seq. 531
3' end seq. ID VHH687Z
3' end seq. 
>VHH687Z.Seq
NNNNNNNNNNATCCAAAGATGATCCAAAAGCTCGTGCTAACTATCTCGCTGATAACCACG
AATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAACGGTGCCA
ATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCG
TTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTA
TCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGGTGGTC
AAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTC
TCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTT
ACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCC
CATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATT
TACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAA
TTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAA
AAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTT
AAGTCGCACTNAGTTTTCAAA
Length of 3' end seq. 741
Connected seq. ID VHH687P
Connected seq. 
>VHH687P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTA----------ATCCAAAGA
TGATCCAAAAGCTCGTGCTAACTATCTCGCTGATAACCACGAATGGGATAAAAATGATGC
CATGAACATTTGGTCTTTCGGTCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCAC
CAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGC
CACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGA
TGTCACCCTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCG
TCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTT
AGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCG
TAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGC
TCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGG
TCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGA
TAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAAT
CCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCACTNAGTTTTCA
AA
Length of connected seq. 1252
Full length Seq ID -
Full length Seq. -
Length of full length seq. -