>AFO186 (AFO186Q) /CSM/AF/AFO1-D/AFO186Q.Seq.d/
XXXXXXXXXXCGTTTTCACACCAGCATTACGTGCCGTTGGTAANATTGTCACTGGTGAAT
CTAGCCAAACTCAAATAGTCATCGATAATAATGGTGTTGAATTGATCACTGGTCTNTTGG
CCGTTCAAAAGAAATCCATTCGTAAAGAATCATGTTGGGCATTATCAAATATCACCGCTG
GTGAACCATCACAAATCGATGTTGTCGTATCAAATCCAAAGACAGTGACAACCTTGATCA
GTCTTCTCTCCCACAGTGAACATGACATTAAACGTGAAGCATGTTGGGCTTTATCAAACT
CTACCAATAATTCATCAACTAAATCAATTCAAACCTTGGTTCGTCACAATATCTTGAAAC
ATTTTATCGATTTATTAAACTCTCAAGATTTAGTTATCCTTAAAATCGTCTTGGAAGGTT
TAATCAATATCATTAAAGAAGGTGAAAAAACTAAAACTAAAACTGGTGTTAATCCATACG
TTAACTTGATCTCTGAAATGCAAGGTGAATCCATCATTTATGATCTTCAAGAGCATCAAT
CAAAAGATGTTTACAAGAAAGCTTTCGAACTTATTGAATTCTTTGAAAGTTCAGATTATT
CTGATTCTGAAAATTCTGAACCAAATATAAATCAAAATGGTCAATAGAATTTAGNTCAAA
TTATAAT

---VFTPALRAVGXIVTGESSQTQIVIDNNGVELITGLLAVQKKSIRKESCWALSNITAG
EPSQIDVVVSNPKTVTTLISLLSHSEHDIKREACWALSNSTNNSSTKSIQTLVRHNILKH
FIDLLNSQDLVILKIVLEGLINIIKEGEKTKTKTGVNPYVNLISEMQGESIIYDLQEHQS
KDVYKKAFELIEFFESSDYSDSENSEPNINQNGQ*nlxqii

Frame A:
---VFTPALRAVGXIVTGESSQTQIVIDNNGVELITGLLAVQKKSIRKESCWALSNITAG
EPSQIDVVVSNPKTVTTLISLLSHSEHDIKREACWALSNSTNNSSTKSIQTLVRHNILKH
FIDLLNSQDLVILKIVLEGLINIIKEGEKTKTKTGVNPYVNLISEMQGESIIYDLQEHQS
KDVYKKAFELIEFFESSDYSDSENSEPNINQNGQ*nlxqii


Frame B:
---fshqhyvplvxlslvnlaklk*ssiimvln*slvxwpfkrnpfvknhvghyqisplv
nhhksmlsyqiqrq*qp*svfsptvnmtlnvkhvglyqtlpiihqlnqfkpwfvtis*ni
lsiy*tlki*lslksswkv*sislkkvkklklklvlihtlt*slkckvnpsfmifksinq
kmftrklsnllnslkvqiililkilnqi*ikmvnri*xkl*


Frame C:
---fhtsitcrw*xchw*i*pnsnshr**wc*idhwsxgrskeihs*rimlgiikyhrw*
titnrccrikskdsdnldqsslpq*t*h*t*smlgfiklyq*fin*insnlgssqyletf
yrfiklsrfsyp*nrlgrfnqyh*rr*kn*n*nwc*sir*ldl*nar*ihhl*ssrasik
rclqesfrty*il*kfrlf*f*kf*tkykskwsiefxsnyn

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO186 (AFO186Q) /CSM/AF/AFO1-D/AFO186Q.Seq.d/                        848   0.0
SFK791 (SFK791Q) /CSM/SF/SFK7-D/SFK791Q.Seq.d/                        841   0.0
SFG195 (SFG195Q) /CSM/SF/SFG1-D/SFG195Q.Seq.d/                        841   0.0
SFE893 (SFE893Q) /CSM/SF/SFE8-D/SFE893Q.Seq.d/                        841   0.0
CHD227 (CHD227Q) /CSM/CH/CHD2-B/CHD227Q.Seq.d/                        841   0.0
CHB147 (CHB147Q) /CSM/CH/CHB1-B/CHB147Q.Seq.d/                        841   0.0
CFC222 (CFC222Q) /CSM/CF/CFC2-A/CFC222Q.Seq.d/                        841   0.0
AFG889 (AFG889Q) /CSM/AF/AFG8-D/AFG889Q.Seq.d/                        841   0.0
AFG251 (AFG251Q) /CSM/AF/AFG2-C/AFG251Q.Seq.d/                        841   0.0
AFA661 (AFA661Q) /CSM/AF/AFA6-C/AFA661Q.Seq.d/                        841   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC115684|AC115684.2 Dictyostelium discoideum chromosome 2 map 3108975-3191114 strain AX4, complete sequence.   841  0.0  3
AL402370|AL402370.1 T3 end of clone AT0AA001E07 of library AT0AA from strain CBS 4311 of Saccharomyces servazzii.    46  0.55  1
BX571703|BX571703.1 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone DKEY-188E21.    40  0.65  5
AL935163|AL935163.7 Zebrafish DNA sequence from clone CH211-206K20.    40  1.1  4
AF529881|AF529881.1 Plasmodium falciparum 3D7 karyopherin alpha gene, complete cds.    38  1.1  2
AC011829|AC011829.7 Homo sapiens chromosome 11 clone RP11-72M10 map 11, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    44  2.2  1
X80176|X80176.1 F.rubripes gene for D3-like dopamine receptor.    44  2.2  1
AC079103|AC079103.2 Homo sapiens chromosome 11 clone RP11-240P4 map 11, WORKING DRAFT SEQUENCE, 24 unordered pieces.    44  2.2  1
AC116768|AC116768.5 Mus musculus clone RP23-395P10, WORKING DRAFT SEQUENCE, 7 ordered pieces.    44  2.2  1
AL138652|AL138652.1 Arabidopsis thaliana DNA chromosome 3, BAC clone T18B22.    38  3.0  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q76P29) RecName: Full=Importin subunit alpha-B; AltName: Full=K...   137   3e-31
AE017344_469(AE017344|pid:none) Cryptococcus neoformans var. neo...   130   3e-29
CP000881_79(CP000881|pid:none) Hemiselmis andersenii chromosome ...   130   3e-29
CP000589_311(CP000589|pid:none) Ostreococcus lucimarinus CCE9901...   130   3e-29
(O94374) RecName: Full=Importin subunit alpha-2; AltName: Full=K...   130   3e-29
BT063489_1(BT063489|pid:none) Zea mays full-length cDNA clone ZM...   129   6e-29
BT041626_1(BT041626|pid:none) Zea mays full-length cDNA clone ZM...   129   6e-29
AJ557449_1(AJ557449|pid:none) Xenopus laevis mRNA for importin a...   129   7e-29
(Q60960) RecName: Full=Importin subunit alpha-1; AltName: Full=K...   128   1e-28
(P83953) RecName: Full=Importin subunit alpha-1; AltName: Full=K...   128   1e-28

 psg: 0.89  gvh: 0.43  alm: 0.35  top: 0.40  tms: 0.00  mit: 0.31  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: vesicles of secretory system
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial

>> prediction for AFO186 is cyt

>AFO186Z.Seq
----------CGTTTTCACACCAGCATTACGTGCCGTTGGTAANATTGTCACTGGTGAAT
CTAGCCAAACTCAAATAGTCATCGATAATAATGGTGTTGAATTGATCACTGGTCTNTTGG
CCGTTCAAAAGAAATCCATTCGTAAAGAATCATGTTGGGCATTATCAAATATCACCGCTG
GTGAACCATCACAAATCGATGTTGTCGTATCAAATCCAAAGACAGTGACAACCTTGATCA
GTCTTCTCTCCCACAGTGAACATGACATTAAACGTGAAGCATGTTGGGCTTTATCAAACT
CTACCAATAATTCATCAACTAAATCAATTCAAACCTTGGTTCGTCACAATATCTTGAAAC
ATTTTATCGATTTATTAAACTCTCAAGATTTAGTTATCCTTAAAATCGTCTTGGAAGGTT
TAATCAATATCATTAAAGAAGGTGAAAAAACTAAAACTAAAACTGGTGTTAATCCATACG
TTAACTTGATCTCTGAAATGCAAGGTGAATCCATCATTTATGATCTTCAAGAGCATCAAT
CAAAAGATGTTTACAAGAAAGCTTTCGAACTTATTGAATTCTTTGAAAGTTCAGATTATT
CTGATTCTGAAAATTCTGAACCAAATATAAATCAAAATGGTCAATAGAATTTAGNTCAAA
TTATAAT