>AFO182 (AFO182Q) /CSM/AF/AFO1-D/AFO182Q.Seq.d/
XXXXXXXXXXTCAACCAGAACAAACTAAAAGATTAGCTGAAAAATTAGGTTTCTCAAAAA
AAAATATTTCAATGGCTCAAGATCAAATGAAAAAATTATATGACTTTTTCATTAAGAATG
ATTGTACATTAGTAGAGATTAACCCATTAGCAGAGACAGCATCTGGTGATGTATTATGTA
TGGATGCCAAATTGAATTTCGATGATAATGCTGCATTCCGTCATCCAGATATTTTCAAAT
TAAGAGATAAATCTCAAGAGGATCCACGTGAAGTTAAAGCAGCAGAGTTTGATTTGAACT
ACATTGGTTTGGATGGCAATATTGGTTGTTTAGTAAATGGTGCAGGTTTAGCAATGGCAT
CAATGGATATCATTAAACTCTATGGCGGTAGCCCAGCAAACTTTTTAGATGTTGGTGGTG
GTGCCACTCAGAAACAAGTCACTGAAGCAATCAAACTCATCAGTAGCGATAAAAAAGTTA
AATCAATTTTAGTCAATATTTTCGGTGGTATCATGAAATGTGATGTCATTGCTTTAGGTA
TCATCGCTGCTTTGAAGGAGTTATCAATCGCCACCCCATTAGTAGTTCGTCTCCAAGGTA
CCAACGTTGAAGCTGCAAAGAAAATCATGTAA

---QPEQTKRLAEKLGFSKKNISMAQDQMKKLYDFFIKNDCTLVEINPLAETASGDVLCM
DAKLNFDDNAAFRHPDIFKLRDKSQEDPREVKAAEFDLNYIGLDGNIGCLVNGAGLAMAS
MDIIKLYGGSPANFLDVGGGATQKQVTEAIKLISSDKKVKSILVNIFGGIMKCDVIALGI
IAALKELSIATPLVVRLQGTNVEAAKKIM*

Frame A:
---QPEQTKRLAEKLGFSKKNISMAQDQMKKLYDFFIKNDCTLVEINPLAETASGDVLCM
DAKLNFDDNAAFRHPDIFKLRDKSQEDPREVKAAEFDLNYIGLDGNIGCLVNGAGLAMAS
MDIIKLYGGSPANFLDVGGGATQKQVTEAIKLISSDKKVKSILVNIFGGIMKCDVIALGI
IAALKELSIATPLVVRLQGTNVEAAKKIM*


Frame B:
---nqnklkd*lkn*vsqkkifqwlkik*knymtfslrmivh**rlth*qrqhlvmyyvw
mpn*ismimlhsviqifsn*einlkrihvklkqqsli*ttlvwmailvv**mvqv*qwhq
wislnsmavaqqtf*mlvvvplrnkslkqsnssvaikklnqf*sifsvvs*nvmsll*vs
sll*rsyqspph**fvskvptlklqrksc


Frame C:
---trtn*kis*kirflkkkyfngsrsnekii*lfh*e*lyisrd*pisrdsiw*cimyg
cqiefr**ccipssryfqikr*isrgst*s*ssrv*felhwfgwqywlfskwcrfsngin
gyh*tlwr*psklfrcwwwchsetsh*snqthq*r*ks*infsqyfrwyhem*chcfryh
rcfegvinrhpisssspryqr*sckenhv

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO182 (AFO182Q) /CSM/AF/AFO1-D/AFO182Q.Seq.d/                       1179   0.0
VFN542 (VFN542Q) /CSM/VF/VFN5-B/VFN542Q.Seq.d/                       1120   0.0
VFM410 (VFM410Q) /CSM/VF/VFM4-A/VFM410Q.Seq.d/                       1120   0.0
VFI611 (VFI611Q) /CSM/VF/VFI6-A/VFI611Q.Seq.d/                       1120   0.0
AFE579 (AFE579Q) /CSM/AF/AFE5-D/AFE579Q.Seq.d/                       1112   0.0
AFL226 (AFL226Q) /CSM/AF/AFL2-B/AFL226Q.Seq.d/                       1090   0.0
SLE579 (SLE579Q) /CSM/SL/SLE5-D/SLE579Q.Seq.d/                       1021   0.0
CFD402 (CFD402Q) /CSM/CF/CFD4-A/CFD402Q.Seq.d/                       1003   0.0
AFB805 (AFB805Q) /CSM/AF/AFB8-A/AFB805Q.Seq.d/                        983   0.0
VFA849 (VFA849Q) /CSM/VF/VFA8-C/VFA849Q.Seq.d/                        963   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC115594|AC115594.2 Dictyostelium discoideum chromosome 2 map 4071862-4101005 strain AX4, complete sequence.  1102  0.0  2
AW053653|AW053653.1 L30-1426T3 Ice plant Lambda Uni-Zap XR expression library, 30 hours NaCl treatment Mesembryanthemum crystallinum cDNA clone L30-1426 5', mRNA sequence.    54  2e-22  5
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    62  1e-20  6
BI127167|BI127167.1 G003P42Y Populus cambium cDNA library Populus tremula x Populus tremuloides cDNA, mRNA sequence.    58  2e-16  3
CB819690|CB819690.1 EST 682 Green Apricot Fruit Lambda Zap II Library Prunus armeniaca cDNA clone ba003j04 3', mRNA sequence.    54  3e-16  4
BQ966505|BQ966505.1 QHB27A24.yg.ab1 QH_ABCDI sunflower RHA801 Helianthus annuus cDNA clone QHB27A24, mRNA sequence.    72  3e-16  3
AP001515|AP001515.2 Bacillus halodurans genomic DNA, section 9/14.    66  2e-15  3
BM276594|BM276594.1 Yv4-10 Scabies lambda zap express cDNA library Sarcoptes scabiei cDNA, mRNA sequence.    56  3e-15  4
AF493055|AF493055.1 Neocallimastix patriciarum succinyl-CoA synthetase, beta subunit (scsB) mRNA, complete cds.    46  9e-14  5
BQ969678|BQ969678.1 QHB39D22.yg.ab1 QH_ABCDI sunflower RHA801 Helianthus annuus cDNA clone QHB39D22, mRNA sequence.    62  2e-13  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC115594_9(AC115594|pid:none) Dictyostelium discoideum chromosom...   404   e-111
(Q55AI5) RecName: Full=Succinyl-CoA ligase [ADP-forming] subunit...   404   e-111
BC118443_1(BC118443|pid:none) Bos taurus succinate-CoA ligase, A...   272   6e-72
AJ851731_1(AJ851731|pid:none) Gallus gallus mRNA for hypothetica...   270   2e-71
BC166998_1(BC166998|pid:none) Rattus norvegicus succinate-Coenzy...   270   3e-71
BC059701_1(BC059701|pid:none) Danio rerio succinate-CoA ligase, ...   270   3e-71
AF058955_1(AF058955|pid:none) Mus musculus ATP-specific succinyl...   269   4e-71
(O97580) RecName: Full=Succinyl-CoA ligase [ADP-forming] subunit...   269   4e-71
AK150594_1(AK150594|pid:none) Mus musculus bone marrow macrophag...   269   4e-71
(Q4R517) RecName: Full=Succinyl-CoA ligase [ADP-forming] subunit...   267   2e-70

 psg: 0.64  gvh: 0.48  alm: 0.30  top: 0.40  tms: 0.07  mit: 0.28  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: mitochondrial
	 20.0 %: cytoplasmic
	 20.0 %: nuclear
	 12.0 %: endoplasmic reticulum
	  8.0 %: Golgi
	  4.0 %: vacuolar
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for AFO182 is mit

>AFO182Z.Seq
----------TCAACCAGAACAAACTAAAAGATTAGCTGAAAAATTAGGTTTCTCAAAAA
AAAATATTTCAATGGCTCAAGATCAAATGAAAAAATTATATGACTTTTTCATTAAGAATG
ATTGTACATTAGTAGAGATTAACCCATTAGCAGAGACAGCATCTGGTGATGTATTATGTA
TGGATGCCAAATTGAATTTCGATGATAATGCTGCATTCCGTCATCCAGATATTTTCAAAT
TAAGAGATAAATCTCAAGAGGATCCACGTGAAGTTAAAGCAGCAGAGTTTGATTTGAACT
ACATTGGTTTGGATGGCAATATTGGTTGTTTAGTAAATGGTGCAGGTTTAGCAATGGCAT
CAATGGATATCATTAAACTCTATGGCGGTAGCCCAGCAAACTTTTTAGATGTTGGTGGTG
GTGCCACTCAGAAACAAGTCACTGAAGCAATCAAACTCATCAGTAGCGATAAAAAAGTTA
AATCAATTTTAGTCAATATTTTCGGTGGTATCATGAAATGTGATGTCATTGCTTTAGGTA
TCATCGCTGCTTTGAAGGAGTTATCAATCGCCACCCCATTAGTAGTTCGTCTCCAAGGTA
CCAACGTTGAAGCTGCAAAGAAAATCATGTAA