>AFO181 (AFO181Q) /CSM/AF/AFO1-D/AFO181Q.Seq.d/
ATATATTTTATTTGGGAAAAATAAATAAATAAATAAATAAATATGTCAGCTCCAGTTTTA
ACCACCAGTAGTGGTTCACCAATTGATAACAATTTAAACTCAATGACCGCAGGTGTTAAT
GGTCCAATTTTAATTCAAGATTTCACTTTAATTGATAAACTTGCTCATTTCGATCGTGAA
CGTATCCCAGAACGTGTTGTACATGCTAAAGGTGCTGGTGCCCATGGTTATTTCGAAGTT
ACCTCAAGTGATGTACCAAAATGGTGTAAAGCTAAATTCTTAAATAAAGTTGGTAAGAGA
ACACCAATCTTCGCCCGTTTCTCTACTGTTGGTGGTGAAAAAGGTTCATCCGATTCTGAA
AGAGATCCAAGAGGTTTCGCTGTCAAATTTTATACTGAAGAAGGTAACTTTGATATGGTC
GGTAATAATACTCCAGTTTTCTTTATTCGTGATCCATCAAAATTCCCAGATTTCATTCAT
ACACAAAAACGTAATCCACAAACAAATTGTAAAGATCCAAATATGTTTTGGGATTTTTTA
GGTCAAACACCAGAATCAACACATCAAGTATCAATTTTATTCTCTGATAGAGGTACACCA
AAATCATATCGTCATATGCATGGTTTCTCATCACATACCTTAAAATTTGTTAATXXXXXX
XXXXAGTTTCCATTCAATTGATGGAATTTGAAGATGCCTTAAAGTATCGTTTCAATCCAT
TCGATGTAACAAAGATTTGGTCACACAAAGACTATCCATTAATTCAAATTGGTCGTATGG
TTTTGGATCGTAATCCAGAGAATTACTTTGCTGAAGTTGAACAAGCTGCCTTCTCACCAT
CCCACATGGTTCCAGGTATTGAACCATCACCAGATAAGATGTTACAAGGTCGTCTCTTCT
CTTATCCAGATACTCATCGTCATCGTTTAGGTGTAAACTATCAACAAATCCCAGTCAATT
GTCCATTCGCCGTAAAGGGTGGTGTTAAAAACTACCAACGTGATGGTTTCATGGCTGTCA
ATGGTAATGGTGGTAAAGGTCCAAACTATCAACCAAACTCTTTCGGTGGTCCAGAGCCAC
ATCCAGAATTTGCTCAACATAAATTTGACGTCTCTGGTTTCGCTGCTCGTCAACCATACA
ATCATCCAAATGACGACTTTGTTCAACCAGGTGATTTATATAGACTCATGTCTGAAGATG
CCAAATCTCGTTTCGTCTCTAATTTAGTCGGTCACATGTCCGGTGTCACCATCAAAGAAA
TTCAAGTTCGTGCCGTTTCAAACTTTTACAAGGCTGACAAAGATTTAGGTGCTCGTTTAT
GTAAGGGTCTTGGTATTGATGTTAATNATGTCATCAAATTCGCTGCTAGATCAAACTTGT
AAATCAATATATTAAAA

iyfiwek*ink*INMSAPVLTTSSGSPIDNNLNSMTAGVNGPILIQDFTLIDKLAHFDRE
RIPERVVHAKGAGAHGYFEVTSSDVPKWCKAKFLNKVGKRTPIFARFSTVGGEKGSSDSE
RDPRGFAVKFYTEEGNFDMVGNNTPVFFIRDPSKFPDFIHTQKRNPQTNCKDPNMFWDFL
GQTPESTHQVSILFSDRGTPKSYRHMHGFSSHTLKFVN---

---VSIQLMEFEDALKYRFNPFDVTKIWSHKDYPLIQIGRMVLDRNPENYFAEVEQAAFS
PSHMVPGIEPSPDKMLQGRLFSYPDTHRHRLGVNYQQIPVNCPFAVKGGVKNYQRDGFMA
VNGNGGKGPNYQPNSFGGPEPHPEFAQHKFDVSGFAARQPYNHPNDDFVQPGDLYRLMSE
DAKSRFVSNLVGHMSGVTIKEIQVRAVSNFYKADKDLGARLCKGLGIDVNXVIKFAARSN
L*inilk

Frame A:
iyfiwek*ink*INMSAPVLTTSSGSPIDNNLNSMTAGVNGPILIQDFTLIDKLAHFDRE
RIPERVVHAKGAGAHGYFEVTSSDVPKWCKAKFLNKVGKRTPIFARFSTVGGEKGSSDSE
RDPRGFAVKFYTEEGNFDMVGNNTPVFFIRDPSKFPDFIHTQKRNPQTNCKDPNMFWDFL
GQTPESTHQVSILFSDRGTPKSYRHMHGFSSHTLKFVN---

---sfhsidgi*rclkvsfqsircnkdlvtqrlsinsnwsygfgs*srellc*s*tscll
tiphgsry*titr*dvtrsslllsryssssfrcklstnpsqlsirrkgwc*klpt*wfhg
cqw*ww*rsklstklfrwsratsricst*i*rlwfrcsstiqssk*rlcstr*fi*thv*
rcqisfrl*fsrshvrchhqrnssscrfkllqg*qrfrcsfm*gswy*c*xchqirc*ik
lvnqyik

Frame B:
yilfgknk*ink*icqlqf*ppvvvhqliti*tq*pqvlmvqf*fkisl*linllisivn
vsqnvlymlkvlvpmvisklpqvmyqngvklns*iklvrehqsspvslllvvkkvhpilk
eiqevslsnfilkkvtliwsviilqfslfvihqnsqisfihknvihkqivkiqicfgif*
vkhqnqhikyqfyslievhqnhivicmvshhip*nll---

---VSIQLMEFEDALKYRFNPFDVTKIWSHKDYPLIQIGRMVLDRNPENYFAEVEQAAFS
PSHMVPGIEPSPDKMLQGRLFSYPDTHRHRLGVNYQQIPVNCPFAVKGGVKNYQRDGFMA
VNGNGGKGPNYQPNSFGGPEPHPEFAQHKFDVSGFAARQPYNHPNDDFVQPGDLYRLMSE
DAKSRFVSNLVGHMSGVTIKEIQVRAVSNFYKADKDLGARLCKGLGIDVNXVIKFAARSN
L*inilk

Frame C:
ifylgkink*inkyvsssfnhq*wftn**qfklndrrc*wsnfnsrfhfn**tcsfrs*t
yprtcctc*rcwcpwlfrsylk*ctkmv*s*ilk*sw*entnlrpflycww*krfirf*k
rskrfrcqily*rr*l*ygr**yssflys*sikiprfhsytkt*stnkl*rskyvlgffr
sntrintssinfil**rytkiissyawflitylkic*---

---fpfn*wnlkmp*sivsihsm*qrfghtktih*fklvvwfwiviqritllklnklpsh
hptwfqvlnhhqircykvvssliqiliviv*v*tinksqsivhsp*rvvlkttnvmvswl
smvmvvkvqtinqtlsvvqshiqnllninltslvsllvnhtiiqmttlfnqviyidsclk
mpnlvssli*svtcpvspskkfkfvpfqtftrltki*vlvyvrvlvlmlxmssnslldqt
cksiy*

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO181 (AFO181Q) /CSM/AF/AFO1-D/AFO181Q.Seq.d/                       2619   0.0
SFJ685 (SFJ685Q) /CSM/SF/SFJ6-D/SFJ685Q.Seq.d/                       1439   0.0
FC-142 (FC-142Q) /CSM/CG/CG-FC/FC-142Q.Seq.d/                        1439   0.0
CFC537 (CFC537Q) /CSM/CF/CFC5-B/CFC537Q.Seq.d/                       1439   0.0
AFG841 (AFG841Q) /CSM/AF/AFG8-B/AFG841Q.Seq.d/                       1439   0.0
AFE580 (AFE580Q) /CSM/AF/AFE5-D/AFE580Q.Seq.d/                       1439   0.0
SFK640 (SFK640Q) /CSM/SF/SFK6-B/SFK640Q.Seq.d/                       1437   0.0
AFN369 (AFN369Q) /CSM/AF/AFN3-C/AFN369Q.Seq.d/                       1435   0.0
AFM195 (AFM195Q) /CSM/AF/AFM1-D/AFM195Q.Seq.d/                       1435   0.0
AFM764 (AFM764Q) /CSM/AF/AFM7-C/AFM764Q.Seq.d/                       1431   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF090443|AF090443.1 Dictyostelium discoideum catalase (cat) mRNA, complete cds.  1439  0.0  2
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.  1439  0.0  4
AB064338|AB064338.1 Candida boidinii CTA1 gene for peroxisomal catalase, complete cds.   111  6e-65  7
AP004595|AP004595.1 Oceanobacillus iheyensis HTE831 genomic DNA, section 3/13.    72  2e-34  7
E65314|E65314.1 Novel catalase, gene of said catalase and composition containing  said  catalase, and process for preparing catalase using the genetic engineering  technology.    78  8e-31  4
AJ400965|AJ400965.1 Proteus mirabilis ORF1 DNA, katA gene and menE gene, strain PR.    76  2e-27  3
AJ400964|AJ400964.1 Proteus mirabilis ORF1 DNA, katA gene and menE gene, wild type.    76  2e-27  3
AX536932|AX536932.1 Sequence 533 from Patent WO02064766.          94  6e-27  3
AB006327|AB006327.1 Candida albicans mRNA for catalase, complete cds.    86  2e-24  3
U40704|U40704.1 Candida albicans catalase (Cat) gene, complete cds.    78  3e-24  3

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF090443_1(AF090443|pid:none) Dictyostelium discoideum catalase ...   493   e-138
AY378080_1(AY378080|pid:none) Tetrahymena thermophila putative c...   326   1e-87
(O93662) RecName: Full=Catalase;          EC=1.11.1.6;  &AJ00593...   321   4e-86
CP000300_481(CP000300|pid:none) Methanococcoides burtonii DSM 62...   315   2e-84
(Q9XZD5) RecName: Full=Peroxisomal catalase;          EC=1.11.1....   312   2e-83
CQ796333_1(CQ796333|pid:none) Sequence 109 from Patent WO2004027...   306   8e-82
AE017333_3935(AE017333|pid:none) Bacillus licheniformis DSM 13, ...   306   8e-82
CP000560_826(CP000560|pid:none) Bacillus amyloliquefaciens FZB42...   301   5e-80
(P26901) RecName: Full=Vegetative catalase;          EC=1.11.1.6...   300   8e-80
(P90682) RecName: Full=Catalase;          EC=1.11.1.6;  &Y10611_...   299   1e-79

 psg: 0.89  gvh: 0.57  alm: 0.44  top: 0.40  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 28.0 %: nuclear
	 24.0 %: cytoplasmic
	 20.0 %: extracellular, including cell wall
	 20.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for AFO181 is nuc

>AFO181F.Seq
ATATATTTTATTTGGGAAAAATAAATAAATAAATAAATAAATATGTCAGCTCCAGTTTTA
ACCACCAGTAGTGGTTCACCAATTGATAACAATTTAAACTCAATGACCGCAGGTGTTAAT
GGTCCAATTTTAATTCAAGATTTCACTTTAATTGATAAACTTGCTCATTTCGATCGTGAA
CGTATCCCAGAACGTGTTGTACATGCTAAAGGTGCTGGTGCCCATGGTTATTTCGAAGTT
ACCTCAAGTGATGTACCAAAATGGTGTAAAGCTAAATTCTTAAATAAAGTTGGTAAGAGA
ACACCAATCTTCGCCCGTTTCTCTACTGTTGGTGGTGAAAAAGGTTCATCCGATTCTGAA
AGAGATCCAAGAGGTTTCGCTGTCAAATTTTATACTGAAGAAGGTAACTTTGATATGGTC
GGTAATAATACTCCAGTTTTCTTTATTCGTGATCCATCAAAATTCCCAGATTTCATTCAT
ACACAAAAACGTAATCCACAAACAAATTGTAAAGATCCAAATATGTTTTGGGATTTTTTA
GGTCAAACACCAGAATCAACACATCAAGTATCAATTTTATTCTCTGATAGAGGTACACCA
AAATCATATCGTCATATGCATGGTTTCTCATCACATACCTTAAAATTTGTTAAT------
----

>AFO181Z.Seq
----------AGTTTCCATTCAATTGATGGAATTTGAAGATGCCTTAAAGTATCGTTTCA
ATCCATTCGATGTAACAAAGATTTGGTCACACAAAGACTATCCATTAATTCAAATTGGTC
GTATGGTTTTGGATCGTAATCCAGAGAATTACTTTGCTGAAGTTGAACAAGCTGCCTTCT
CACCATCCCACATGGTTCCAGGTATTGAACCATCACCAGATAAGATGTTACAAGGTCGTC
TCTTCTCTTATCCAGATACTCATCGTCATCGTTTAGGTGTAAACTATCAACAAATCCCAG
TCAATTGTCCATTCGCCGTAAAGGGTGGTGTTAAAAACTACCAACGTGATGGTTTCATGG
CTGTCAATGGTAATGGTGGTAAAGGTCCAAACTATCAACCAAACTCTTTCGGTGGTCCAG
AGCCACATCCAGAATTTGCTCAACATAAATTTGACGTCTCTGGTTTCGCTGCTCGTCAAC
CATACAATCATCCAAATGACGACTTTGTTCAACCAGGTGATTTATATAGACTCATGTCTG
AAGATGCCAAATCTCGTTTCGTCTCTAATTTAGTCGGTCACATGTCCGGTGTCACCATCA
AAGAAATTCAAGTTCGTGCCGTTTCAAACTTTTACAAGGCTGACAAAGATTTAGGTGCTC
GTTTATGTAAGGGTCTTGGTATTGATGTTAATNATGTCATCAAATTCGCTGCTAGATCAA
ACTTGTAAATCAATATATTAAAA

>AFO181P.Seq
ATATATTTTATTTGGGAAAAATAAATAAATAAATAAATAAATATGTCAGCTCCAGTTTTA
ACCACCAGTAGTGGTTCACCAATTGATAACAATTTAAACTCAATGACCGCAGGTGTTAAT
GGTCCAATTTTAATTCAAGATTTCACTTTAATTGATAAACTTGCTCATTTCGATCGTGAA
CGTATCCCAGAACGTGTTGTACATGCTAAAGGTGCTGGTGCCCATGGTTATTTCGAAGTT
ACCTCAAGTGATGTACCAAAATGGTGTAAAGCTAAATTCTTAAATAAAGTTGGTAAGAGA
ACACCAATCTTCGCCCGTTTCTCTACTGTTGGTGGTGAAAAAGGTTCATCCGATTCTGAA
AGAGATCCAAGAGGTTTCGCTGTCAAATTTTATACTGAAGAAGGTAACTTTGATATGGTC
GGTAATAATACTCCAGTTTTCTTTATTCGTGATCCATCAAAATTCCCAGATTTCATTCAT
ACACAAAAACGTAATCCACAAACAAATTGTAAAGATCCAAATATGTTTTGGGATTTTTTA
GGTCAAACACCAGAATCAACACATCAAGTATCAATTTTATTCTCTGATAGAGGTACACCA
AAATCATATCGTCATATGCATGGTTTCTCATCACATACCTTAAAATTTGTTAAT------
----AGTTTCCATTCAATTGATGGAATTTGAAGATGCCTTAAAGTATCGTTTCAATCCAT
TCGATGTAACAAAGATTTGGTCACACAAAGACTATCCATTAATTCAAATTGGTCGTATGG
TTTTGGATCGTAATCCAGAGAATTACTTTGCTGAAGTTGAACAAGCTGCCTTCTCACCAT
CCCACATGGTTCCAGGTATTGAACCATCACCAGATAAGATGTTACAAGGTCGTCTCTTCT
CTTATCCAGATACTCATCGTCATCGTTTAGGTGTAAACTATCAACAAATCCCAGTCAATT
GTCCATTCGCCGTAAAGGGTGGTGTTAAAAACTACCAACGTGATGGTTTCATGGCTGTCA
ATGGTAATGGTGGTAAAGGTCCAAACTATCAACCAAACTCTTTCGGTGGTCCAGAGCCAC
ATCCAGAATTTGCTCAACATAAATTTGACGTCTCTGGTTTCGCTGCTCGTCAACCATACA
ATCATCCAAATGACGACTTTGTTCAACCAGGTGATTTATATAGACTCATGTCTGAAGATG
CCAAATCTCGTTTCGTCTCTAATTTAGTCGGTCACATGTCCGGTGTCACCATCAAAGAAA
TTCAAGTTCGTGCCGTTTCAAACTTTTACAAGGCTGACAAAGATTTAGGTGCTCGTTTAT
GTAAGGGTCTTGGTATTGATGTTAATNATGTCATCAAATTCGCTGCTAGATCAAACTTGT
AAATCAATATATTAAAA