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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHH618
Library VH
(Link to library)
Clone ID VHH618
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13955-1
Original site URL
Representative seq. ID VHH618P
(Link to Original site)
Representative DNA sequence 
>VHH618 (VHH618Q) /CSM/VH/VHH6-A/VHH618Q.Seq.d/
ATTTTTTAACCTCCATACTTTCACACACATAAAATTCAAATAAATATAAATATATCTATA
ATTTTTATATATATATATTTTTAAACTTTATTTTTTTTATATAATAACAAAATTTTTATT
TATTTATTTAATTTTAATAATAACAATTAAAAAATGATTGAAGGTAGTGGTAGTAAAACT
CCAAAGAGAAACTCTGATGAAATTTCAGTCAAGAGTTTAAGTATTCATGAAGCCAATACA
TTATCATCTGAACATGAACATGAAAGTGGAAATGAAGAATTAACCCTCAGTCAAATTGTT
ATTAGATCAGACTTTGCCAATGAATCCTCTGAAAGACAATCAAAGATCATTTTGGATAGA
TTCAATCAAGGTATTGGTAAAAACTCTGCATTTACAGATTCACCAGCACTTGGTCGTGGT
CTTTCACGTGTTGATGTTAGTTATTTTGTTATGGGTCATCGTAATCAAGTTGCAGAGTTA
TATGATCCACGTGGTCAACGTTTCAGTACTGTATTAACACCAGATCCAACTGTTAACATT
AAATACGTTGGTCCAGTTTCAAAGACTGCACACTACTTTGGTGCCTATGGTAAACATGTC
TTXXXXXXXXXXCGTGTAAAGAGTCATTTGGCTAGAGANTTATTCGATTATGGTGTTGAG
TNATCACGTTTACAAATTGAAACTATGAAAGTTTTAGATACTGAAATCGCAAAGAAACTC
GCNGGCCAATCTGTCACCTCTGCAGAGTTTACCCCTAAACAAGCAACCCTTGTCAAAGAG
TATGATATTA
sequence update 2002.10.25
Translated Amino Acid sequence 
if*ppyfhthkiqininisiifiyiyf*tlfflynnkifiylfnfnnnn*KMIEGSGSKT
PKRNSDEISVKSLSIHEANTLSSEHEHESGNEELTLSQIVIRSDFANESSERQSKIILDR
FNQGIGKNSAFTDSPALGRGLSRVDVSYFVMGHRNQVAELYDPRGQRFSTVLTPDPTVNI
KYVGPVSKTAHYFGAYGKHV---

---RVKSHLARXLFDYGVEXSRLQIETMKVLDTEIAKKLAGQSVTSAEFTPKQATLVKEY
DI
Translated Amino Acid sequence (All Frames) 
Frame A:
if*ppyfhthkiqininisiifiyiyf*tlfflynnkifiylfnfnnnn*KMIEGSGSKT
PKRNSDEISVKSLSIHEANTLSSEHEHESGNEELTLSQIVIRSDFANESSERQSKIILDR
FNQGIGKNSAFTDSPALGRGLSRVDVSYFVMGHRNQVAELYDPRGQRFSTVLTPDPTVNI
KYVGPVSKTAHYFGAYGKHV---

---RVKSHLARXLFDYGVEXSRLQIETMKVLDTEIAKKLAGQSVTSAEFTPKQATLVKEY
DI

Frame B:
ffnlhtfthikfk*i*iyl*flyiyifklyffyiitkflfiyliliitikk*lkvvvvkl
qretlmkfqsrv*vfmkpihyhlnmnmkvemkn*psvkllldqtlpmnplkdnqrsfwid
sikvlvktlhlqihqhlvvvfhvlmlvillwviviklqsymihvvnvsvly*hqiqlltl
ntlvqfqrlhttlvpmvnms---

---v*rviwlexysimvlsxhvyklkl*kf*ilksqrnsxanlsplqslplnkqplsksm
il

Frame C:
fltsilsht*nsnkykyiynfyiyiflnfiffi**qnfylfi*f***qlknd*r*w**ns
kekl**nfsqefkys*sqyiii*t*t*kwk*rinpqsncy*irlcq*il*ktikdhfg*i
qsryw*klciyrftstwswsftc*c*lfcygss*sscrvi*stwstfqycintrsnc*h*
irwssfkdctllwclw*tcl---

---ckesfg*rxirlwc*vitftn*nyesfry*nrketrxpichlcrvyp*tsnpcqrv*
y
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHH618 (VHH618Q) /CSM/VH/VHH6-A/VHH618Q.Seq.d/                       1183   0.0
SFJ283 (SFJ283Q) /CSM/SF/SFJ2-D/SFJ283Q.Seq.d/                        888   0.0
AHI815 (AHI815Q) /CSM/AH/AHI8-A/AHI815Q.Seq.d/                        888   0.0
VHM373 (VHM373Q) /CSM/VH/VHM3-D/VHM373Q.Seq.d/                        886   0.0
FC-IC1242 (FC-IC1242Q) /CSM/FC-IC/FC-IC1242Q.Seq.d/                   694   0.0
SLA374 (SLA374Q) /CSM/SL/SLA3-D/SLA374Q.Seq.d/                        408   e-113
SFB121 (SFB121Q) /CSM/SF/SFB1-A/SFB121Q.Seq.d/                        327   1e-88
VHJ147 (VHJ147Q) /CSM/VH/VHJ1-B/VHJ147Q.Seq.d/                        315   6e-85
FC-IC0368 (FC-IC0368Q) /CSM/FC-IC/FC-IC0368Q.Seq.d/                   188   9e-47
SSM704 (SSM704Q) /CSM/SS/SSM7-A/SSM704Q.Seq.d/                         72   1e-11
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AF014049) Dictyostelium discoideum vacuolin A (vac A) mRNA,...   886   0.0    2
(BJ422472) Dictyostelium discoideum cDNA clone:ddv46c06, 5' ...   886   0.0    2
(BJ334893) Dictyostelium discoideum cDNA clone:dda48n04, 5' ...   886   0.0    2
(BJ425446) Dictyostelium discoideum cDNA clone:ddv55b19, 5' ...   884   0.0    2
(BJ389709) Dictyostelium discoideum cDNA clone:dds19e22, 5' ...   886   0.0    1
(AU272320) Dictyostelium discoideum gamete cDNA clone:FC-IC1...   698   0.0    2
(AU272319) Dictyostelium discoideum gamete cDNA clone:FC-IC1...   684   0.0    2
(AU060128) Dictyostelium discoideum slug cDNA, clone SLA374.      408   e-109  1
(BJ441214) Dictyostelium discoideum cDNA clone:ddv46c06, 3' ...   335   1e-87  1
(BJ444243) Dictyostelium discoideum cDNA clone:ddv55b19, 3' ...   327   4e-85  1
dna update 2008.11. 9
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54WZ2) RecName: Full=Vacuolin-B;                                    174   2e-42
AF014050_1(AF014050|pid:none) Dictyostelium discoideum vacuolin ...   174   2e-42
(Q54WZ3) RecName: Full=Vacuolin-C;                                    144   2e-33
CP000482_3543(CP000482|pid:none) Pelobacter propionicus DSM 2379...    35   2.8
protein update 2009. 3.10
PSORT 

 psg: 0.32  gvh: 0.43  alm: 0.51  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: cytoplasmic
	 16.0 %: mitochondrial
	 12.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal

>> prediction for VHH618 is cyt
5' end seq. ID VHH618F
5' end seq. 
>VHH618F.Seq
ATTTTTTAACCTCCATACTTTCACACACATAAAATTCAAATAAATATAAATATATCTATA
ATTTTTATATATATATATTTTTAAACTTTATTTTTTTTATATAATAACAAAATTTTTATT
TATTTATTTAATTTTAATAATAACAATTAAAAAATGATTGAAGGTAGTGGTAGTAAAACT
CCAAAGAGAAACTCTGATGAAATTTCAGTCAAGAGTTTAAGTATTCATGAAGCCAATACA
TTATCATCTGAACATGAACATGAAAGTGGAAATGAAGAATTAACCCTCAGTCAAATTGTT
ATTAGATCAGACTTTGCCAATGAATCCTCTGAAAGACAATCAAAGATCATTTTGGATAGA
TTCAATCAAGGTATTGGTAAAAACTCTGCATTTACAGATTCACCAGCACTTGGTCGTGGT
CTTTCACGTGTTGATGTTAGTTATTTTGTTATGGGTCATCGTAATCAAGTTGCAGAGTTA
TATGATCCACGTGGTCAACGTTTCAGTACTGTATTAACACCAGATCCAACTGTTAACATT
AAATACGTTGGTCCAGTTTCAAAGACTGCACACTACTTTGGTGCCTATGGTAAACATGTC
TTNNNNNNNNNN
Length of 5' end seq. 612
3' end seq. ID VHH618Z
3' end seq. 
>VHH618Z.Seq
NNNNNNNNNNCGTGTAAAGAGTCATTTGGCTAGAGANTTATTCGATTATGGTGTTGAGTN
ATCACGTTTACAAATTGAAACTATGAAAGTTTTAGATACTGAAATCGCAAAGAAACTCGC
NGGCCAATCTGTCACCTCTGCAGAGTTTACCCCTAAACAAGCAACCCTTGTCAAAGAGTA
TGATATTA
Length of 3' end seq. 188
Connected seq. ID VHH618P
Connected seq. 
>VHH618P.Seq
ATTTTTTAACCTCCATACTTTCACACACATAAAATTCAAATAAATATAAATATATCTATA
ATTTTTATATATATATATTTTTAAACTTTATTTTTTTTATATAATAACAAAATTTTTATT
TATTTATTTAATTTTAATAATAACAATTAAAAAATGATTGAAGGTAGTGGTAGTAAAACT
CCAAAGAGAAACTCTGATGAAATTTCAGTCAAGAGTTTAAGTATTCATGAAGCCAATACA
TTATCATCTGAACATGAACATGAAAGTGGAAATGAAGAATTAACCCTCAGTCAAATTGTT
ATTAGATCAGACTTTGCCAATGAATCCTCTGAAAGACAATCAAAGATCATTTTGGATAGA
TTCAATCAAGGTATTGGTAAAAACTCTGCATTTACAGATTCACCAGCACTTGGTCGTGGT
CTTTCACGTGTTGATGTTAGTTATTTTGTTATGGGTCATCGTAATCAAGTTGCAGAGTTA
TATGATCCACGTGGTCAACGTTTCAGTACTGTATTAACACCAGATCCAACTGTTAACATT
AAATACGTTGGTCCAGTTTCAAAGACTGCACACTACTTTGGTGCCTATGGTAAACATGTC
TT----------CGTGTAAAGAGTCATTTGGCTAGAGANTTATTCGATTATGGTGTTGAG
TNATCACGTTTACAAATTGAAACTATGAAAGTTTTAGATACTGAAATCGCAAAGAAACTC
GCNGGCCAATCTGTCACCTCTGCAGAGTTTACCCCTAAACAAGCAACCCTTGTCAAAGAG
TATGATATTA
Length of connected seq. 780
Full length Seq ID -
Full length Seq. -
Length of full length seq. -