>AFO165 (AFO165Q) /CSM/AF/AFO1-C/AFO165Q.Seq.d/
ATTTTGTTTCACTTCAAACATTAAAAAAACACATCAAGAGTACTCATGTCTCACTTTGCA
AGAGCTAACATCCCAACACCGGTTAACCAACCAATGTTAAATTATGAAGTTGGTTCAACA
CATAGAAAATTATTAAAAGAAGCATGTGCTAAATTTAGAAACACAACAATCGATATTCCA
TGTGTTGTTGGTGGTAAAGAAATTCGTACTGGTGATGTTCAAAAGCAATTAATTTGCTCT
GATCATAATAAAGTATTGGCCACCTTCCATCAAGCCAATGCCGAATTACTCAAATTAGCC
GTTGAAAATTCCATGGAATCTAAAGCCCAATGGGAATCATTACCATTTGAAGCAAGAAGT
GCAGTTTTCCTTAAAGCTGCAGATTTGTTAAATACCAAGTACCGTTATGATGTCTTAGCT
TCTACTATGCTTGGTCAAGGTAAAACTGTTTGGCAAGCAGAAATTGATGCAGCAGCAGAA
GGTATTGATTTCCTCCGTTTCAATGTTAAATACGCTCAAGAAATTTACCAACAACAACCA
CCAGCCAATTCTGCAGGCTGTTGGAACATTTTAACCTATCAACCATTAXXXXXXXXXXGT
CAAAAATGTTCAGCTTGTTCACGTGCTTACATTCCACAATCTTTATGGCCCCAAATTAAA
GATAGATTAGTTACTGGTGTTAAATCAATGAAAATGGGTCAATCTGATGATTTCTCTTCA
TTCGTTTCAGCTGTCATCGATAAAAACTCTTTCAACAATATTCAATCATACATTGAACAC
GCTAAAGCTTCACCAGATGCTGAAATCATTGTCGGTGGTAAATGTGATAGCTCTGTTGGT
TGGTTTGTTGAACCAACTATCATCCTTGCCAAAGATCCACATTACAAATCAATGGAAGAA
GAAATTTTCGGTCCAGTCTTAACCATTTATGTTTATGAAGATTCTAAATTTGAAGAAACA
TTAAAAATCTGTGATGAAACTTCACCATATGCTTTAACTGGTTCAATCTTTTCAACTTGT
AGATATGCTATTGAAACCGCTCACAAATACTTGAAGAATGCTGCTGGTAACTTTTATATC
AATGATAAATGCACTGGTGCCGTCGTTGGTCAACAACCATTTGGTGGTTCTCGTGCTTCA
GGTACAAACGATAAAGCTGGTTCATCATTAAATCTTTTAAGATGGATCAGCGCCAGAACA
ATCAAAGAAAACTTTGTCCCACTCACTAGTTTCACTTATCCATACAGATGATCCAGAAAA
CTAAA

ilfhfkh*KNTSRVLMSHFARANIPTPVNQPMLNYEVGSTHRKLLKEACAKFRNTTIDIP
CVVGGKEIRTGDVQKQLICSDHNKVLATFHQANAELLKLAVENSMESKAQWESLPFEARS
AVFLKAADLLNTKYRYDVLASTMLGQGKTVWQAEIDAAAEGIDFLRFNVKYAQEIYQQQP
PANSAGCWNILTYQPL---

---QKCSACSRAYIPQSLWPQIKDRLVTGVKSMKMGQSDDFSSFVSAVIDKNSFNNIQSY
IEHAKASPDAEIIVGGKCDSSVGWFVEPTIILAKDPHYKSMEEEIFGPVLTIYVYEDSKF
EETLKICDETSPYALTGSIFSTCRYAIETAHKYLKNAAGNFYINDKCTGAVVGQQPFGGS
RASGTNDKAGSSLNLLRWISARTIKENFVPLTSFTYPYR*srkl

Frame A:
ilfhfkh*KNTSRVLMSHFARANIPTPVNQPMLNYEVGSTHRKLLKEACAKFRNTTIDIP
CVVGGKEIRTGDVQKQLICSDHNKVLATFHQANAELLKLAVENSMESKAQWESLPFEARS
AVFLKAADLLNTKYRYDVLASTMLGQGKTVWQAEIDAAAEGIDFLRFNVKYAQEIYQQQP
PANSAGCWNILTYQPL---

---vknvqlvhvltfhnlygpklkid*llvlnq*kwvnlmislhsfqlssiktlstifnh
tlntlklhqmlkslsvvnviallvgllnqlsslpkihitnqwkkkfsvqs*pfmfmkiln
lkkh*ksvmklhhml*lvqsfqlvdmllkpltnt*rmllvtfisminalvpslvnnhlvv
lvlqvqtiklvhh*if*dgsapeqskktlshslvslihtddpen*

Frame B:
fcftsnikkthqeyscltlqeltsqhrltnqc*imklvqhieny*kkhvlnletqqsifh
vllvvkkfvlvmfksn*faliiikywppsikpmpnysn*plkipwnlkpngnhyhlkqev
qfslklqic*ipstvmms*lllclvkvklfgkqklmqqqkvlissvsmlntlkkftnnnh
qpilqavgtf*pinh---

---skmfslftclhstifmapn*r*isywc*inengsi**flfirfschr*klfqqysii
h*tr*sftrc*nhcrw*m**lcwlvc*tnyhpcqrstlqingrrnfrsslnhlcl*rf*i
*rniknl**nfticfnwfnlfnl*icy*nrsqileeccw*llyq**mhwcrrwsttiwwf
scfrykr*swfiiksfkmdqrqnnqrklcpth*fhlsiqmiqktk

Frame C:
fvslqtlkkhiksthvslcks*hpntg*ptnvkl*swfnt*kiikrsmc*i*khnnrysm
ccww*rnsyw*cskainll*s**sighlpssqcritqisr*kfhgi*spmgiiti*skkc
sfp*scrfvkyqvpl*clsfyyawsr*nclasrn*cssrry*fppfqc*irsrnlptttt
sqfcrllehfnlsti---

---QKCSACSRAYIPQSLWPQIKDRLVTGVKSMKMGQSDDFSSFVSAVIDKNSFNNIQSY
IEHAKASPDAEIIVGGKCDSSVGWFVEPTIILAKDPHYKSMEEEIFGPVLTIYVYEDSKF
EETLKICDETSPYALTGSIFSTCRYAIETAHKYLKNAAGNFYINDKCTGAVVGQQPFGGS
RASGTNDKAGSSLNLLRWISARTIKENFVPLTSFTYPYR*srkl

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO165 (AFO165Q) /CSM/AF/AFO1-C/AFO165Q.Seq.d/                       2407   0.0
VFB850 (VFB850Q) /CSM/VF/VFB8-C/VFB850Q.Seq.d/                       1312   0.0
AFH573 (AFH573Q) /CSM/AF/AFH5-D/AFH573Q.Seq.d/                       1310   0.0
VFG887 (VFG887Q) /CSM/VF/VFG8-D/VFG887Q.Seq.d/                       1302   0.0
VFE114 (VFE114Q) /CSM/VF/VFE1-A/VFE114Q.Seq.d/                       1302   0.0
CFI826 (CFI826Q) /CSM/CF/CFI8-B/CFI826Q.Seq.d/                       1302   0.0
CFF617 (CFF617Q) /CSM/CF/CFF6-A/CFF617Q.Seq.d/                       1302   0.0
AFG621 (AFG621Q) /CSM/AF/AFG6-A/AFG621Q.Seq.d/                       1302   0.0
AFE247 (AFE247Q) /CSM/AF/AFE2-B/AFE247Q.Seq.d/                       1302   0.0
SFK660 (SFK660Q) /CSM/SF/SFK6-C/SFK660Q.Seq.d/                       1300   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AL031786|AL031786.1 S.pombe chromosome II cosmid c24C6.           88  9e-15  4
D89230|D89230.1 Schizosaccharomyces pombe mRNA, partial cds, clone: SY 1373.    80  8e-11  1
AX620724|AX620724.1 Sequence 3687 from Patent WO02094868.         48  1e-08  4
CF192429|CF192429.1 l4g02j2.f1 Cryptococcus neoformans strain B3501 Cryptococcus neoformans var. neoformans cDNA clone l4g02j2 3', mRNA sequence.    70  7e-08  1
AR353913|AR353913.1 Sequence 31 from patent US 6593114.           48  5e-06  4
AL416406|AL416406.1 T3 end of clone AX0AA014C08 of library AX0AA from strain CBS 7064 of Pichia farinosa.    56  1e-05  2
AC074026|AC074026.14 Staphylococcus aureus clone sabac-16, complete sequence.    48  1e-05  6
AX768536|AX768536.1 Sequence 122 from Patent WO03011899.          48  2e-05  3
AL416312|AL416312.1 T7 end of clone AX0AA013D07 of library AX0AA from strain CBS 7064 of Pichia farinosa.    48  6e-05  2
AL393468|AL393468.1 T3 end of clone AR0AA011B08 of library AR0AA from strain CBS 732 of Zygosaccharomyces rouxii.    60  7e-05  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RA2) RecName: Full=Delta-1-pyrroline-5-carboxylate dehydroge...   441   e-122
EF157670_5(EF157670|pid:none) Uncultured bacterium CBNPD1 BAC cl...   297   4e-79
CP001087_1672(CP001087|pid:none) Desulfobacterium autotrophicum ...   291   2e-77
CP000140_2188(CP000140|pid:none) Parabacteroides distasonis ATCC...   291   4e-77
AP009380_1401(AP009380|pid:none) Porphyromonas gingivalis ATCC 3...   275   3e-72
CU466930_1098(CU466930|pid:none) Candidatus Cloacamonas acidamin...   273   7e-72
CU207366_389(CU207366|pid:none) Gramella forsetii KT0803 complet...   272   2e-71
CP000251_828(CP000251|pid:none) Anaeromyxobacter dehalogenans 2C...   272   2e-71
CP001359_872(CP001359|pid:none) Anaeromyxobacter dehalogenans 2C...   271   3e-71
CP001034_1725(CP001034|pid:none) Natranaerobius thermophilus JW/...   271   3e-71

 psg: 0.75  gvh: 0.46  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.47  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 84.0 %: mitochondrial
	  8.0 %: cytoplasmic
	  4.0 %: plasma membrane
	  4.0 %: nuclear

>> prediction for AFO165 is mit

>AFO165F.Seq
ATTTTGTTTCACTTCAAACATTAAAAAAACACATCAAGAGTACTCATGTCTCACTTTGCA
AGAGCTAACATCCCAACACCGGTTAACCAACCAATGTTAAATTATGAAGTTGGTTCAACA
CATAGAAAATTATTAAAAGAAGCATGTGCTAAATTTAGAAACACAACAATCGATATTCCA
TGTGTTGTTGGTGGTAAAGAAATTCGTACTGGTGATGTTCAAAAGCAATTAATTTGCTCT
GATCATAATAAAGTATTGGCCACCTTCCATCAAGCCAATGCCGAATTACTCAAATTAGCC
GTTGAAAATTCCATGGAATCTAAAGCCCAATGGGAATCATTACCATTTGAAGCAAGAAGT
GCAGTTTTCCTTAAAGCTGCAGATTTGTTAAATACCAAGTACCGTTATGATGTCTTAGCT
TCTACTATGCTTGGTCAAGGTAAAACTGTTTGGCAAGCAGAAATTGATGCAGCAGCAGAA
GGTATTGATTTCCTCCGTTTCAATGTTAAATACGCTCAAGAAATTTACCAACAACAACCA
CCAGCCAATTCTGCAGGCTGTTGGAACATTTTAACCTATCAACCATTA----------

>AFO165Z.Seq
----------GTCAAAAATGTTCAGCTTGTTCACGTGCTTACATTCCACAATCTTTATGG
CCCCAAATTAAAGATAGATTAGTTACTGGTGTTAAATCAATGAAAATGGGTCAATCTGAT
GATTTCTCTTCATTCGTTTCAGCTGTCATCGATAAAAACTCTTTCAACAATATTCAATCA
TACATTGAACACGCTAAAGCTTCACCAGATGCTGAAATCATTGTCGGTGGTAAATGTGAT
AGCTCTGTTGGTTGGTTTGTTGAACCAACTATCATCCTTGCCAAAGATCCACATTACAAA
TCAATGGAAGAAGAAATTTTCGGTCCAGTCTTAACCATTTATGTTTATGAAGATTCTAAA
TTTGAAGAAACATTAAAAATCTGTGATGAAACTTCACCATATGCTTTAACTGGTTCAATC
TTTTCAACTTGTAGATATGCTATTGAAACCGCTCACAAATACTTGAAGAATGCTGCTGGT
AACTTTTATATCAATGATAAATGCACTGGTGCCGTCGTTGGTCAACAACCATTTGGTGGT
TCTCGTGCTTCAGGTACAAACGATAAAGCTGGTTCATCATTAAATCTTTTAAGATGGATC
AGCGCCAGAACAATCAAAGAAAACTTTGTCCCACTCACTAGTTTCACTTATCCATACAGA
TGATCCAGAAAACTAAA

>AFO165P.Seq
ATTTTGTTTCACTTCAAACATTAAAAAAACACATCAAGAGTACTCATGTCTCACTTTGCA
AGAGCTAACATCCCAACACCGGTTAACCAACCAATGTTAAATTATGAAGTTGGTTCAACA
CATAGAAAATTATTAAAAGAAGCATGTGCTAAATTTAGAAACACAACAATCGATATTCCA
TGTGTTGTTGGTGGTAAAGAAATTCGTACTGGTGATGTTCAAAAGCAATTAATTTGCTCT
GATCATAATAAAGTATTGGCCACCTTCCATCAAGCCAATGCCGAATTACTCAAATTAGCC
GTTGAAAATTCCATGGAATCTAAAGCCCAATGGGAATCATTACCATTTGAAGCAAGAAGT
GCAGTTTTCCTTAAAGCTGCAGATTTGTTAAATACCAAGTACCGTTATGATGTCTTAGCT
TCTACTATGCTTGGTCAAGGTAAAACTGTTTGGCAAGCAGAAATTGATGCAGCAGCAGAA
GGTATTGATTTCCTCCGTTTCAATGTTAAATACGCTCAAGAAATTTACCAACAACAACCA
CCAGCCAATTCTGCAGGCTGTTGGAACATTTTAACCTATCAACCATTA----------GT
CAAAAATGTTCAGCTTGTTCACGTGCTTACATTCCACAATCTTTATGGCCCCAAATTAAA
GATAGATTAGTTACTGGTGTTAAATCAATGAAAATGGGTCAATCTGATGATTTCTCTTCA
TTCGTTTCAGCTGTCATCGATAAAAACTCTTTCAACAATATTCAATCATACATTGAACAC
GCTAAAGCTTCACCAGATGCTGAAATCATTGTCGGTGGTAAATGTGATAGCTCTGTTGGT
TGGTTTGTTGAACCAACTATCATCCTTGCCAAAGATCCACATTACAAATCAATGGAAGAA
GAAATTTTCGGTCCAGTCTTAACCATTTATGTTTATGAAGATTCTAAATTTGAAGAAACA
TTAAAAATCTGTGATGAAACTTCACCATATGCTTTAACTGGTTCAATCTTTTCAACTTGT
AGATATGCTATTGAAACCGCTCACAAATACTTGAAGAATGCTGCTGGTAACTTTTATATC
AATGATAAATGCACTGGTGCCGTCGTTGGTCAACAACCATTTGGTGGTTCTCGTGCTTCA
GGTACAAACGATAAAGCTGGTTCATCATTAAATCTTTTAAGATGGATCAGCGCCAGAACA
ATCAAAGAAAACTTTGTCCCACTCACTAGTTTCACTTATCCATACAGATGATCCAGAAAA
CTAAA