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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO150
Library AF
(Link to library)
Clone ID AFO150
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15310-1
Original site URL
Representative seq. ID AFO150P
(Link to Original site)
Representative DNA sequence 
>AFO150 (AFO150Q) /CSM/AF/AFO1-C/AFO150Q.Seq.d/
TGGCCTACTGGAAATTATTATACCCCCTCACACTATATATAAATTTACATATATATAAAA
AAACACAAACACAAATAAAAAATTTAAAATGGCAGGAAACAAGAAACAAGTTAAAAAGAA
CACTAAACCAATCGTTGTTGATATTGATGATAAACCATTATTAGATACTTCAAATTTAGA
TACTGCAGTTCAAACAAGTGCATCAACTAAAAAAGACGGAAAAAAAGTTACTGCTACCAC
CACCACCACCACCCCAACTCCAACTCCAACCCCAACACCAACTCCAACAACTACTACCAC
AACAGAAAAAAAAAGTAAAGAAATTGAAATTGNACCAACACCATCAAATGTACCAGCTTC
AATTNCAGCATCAACTTCAAATGTTGNAATTGCACCAGCTGNNGAAGCACAAATNACTGN
TGACTTTNCAGNNGNNCCANANAAAAATNNNNNNAXXXXXXXXXXTNNNNNNTNNTNNNN
NNNNNNNCNNNNNNNNNNNNATGNNTNNTNATNNTCNTNATNTANNNNNTNNNNATCNNA
ANNNNCTNNNANAGTTGNNCCAACTCCANCAACAACCACTCAAAGAACAACCACTACTAA
ATCAACTCCAACTCCAACACCAACTCCAACTCCAGCTGCTACTAAAGTTGTTGAACAACC
AAAAGAAAAATCATCACCAGCACCAGTTAAAAAAGAAAAAGAAATTAANCAAAATAAAAA
AGAATCTGAAGGATTTTTATTCAGTTTAATGGAATCTTTAATTACTCCAGGTGTTCCATC
CGTAGTTTACAAAATCATTTACGTCGCCCTCATTGGTGTTTTATTATTTTCATTAGTACC
ATTATATTACGCTGGTTTAGATAGTATCTACAGTTATGGTGTCATCGCTTTAGTTTTAGG
TCTTGGTATTTCTTTAACTTTATTCATCAGTGAAATTCCAAGATTACAAGCTTCAAAGGA
ACAAAAATCAAAATCTGGTAACAAAAAATCAACTAC
sequence update 2001. 6. 1
Translated Amino Acid sequence 
glleiiipphtiykftyi*KNTNTNKKFKMAGNKKQVKKNTKPIVVDIDDKPLLDTSNLD
TAVQTSASTKKDGKKVTATTTTTTPTPTPTPTPTPTTTTTTEKKSKEIEIXPTPSNVPAS
IXASTSNVXIAPAXEAQXTXDFXXXPXKNXX---

---XXXXXXXXXXXXXXXXSXXXXXXSXXXXXVXPTPXTTTQRTTTTKSTPTPTPTPTPA
ATKVVEQPKEKSSPAPVKKEKEIXQNKKESEGFLFSLMESLITPGVPSVVYKIIYVALIG
VLLFSLVPLYYAGLDSIYSYGVIALVLGLGISLTLFISEIPRLQASKEQKSKSGNKKST
Translated Amino Acid sequence (All Frames) 
Frame A:
wptgnyytpshyi*iyiyikkhkhk*ki*ngrkqets*keh*tnrc*y***tiiryfkfr
ycssnkcin*krrkksycyhhhhhpnsnsnpntnsnnyyhnrkkk*rn*nxtntikctsf
nxsinfkcxnctsxxstnxx*lxxxxxxkxx---

---xxxxxxxxxxxxxxxxxxxxxxxixxxxxsxxnsxnnhsknnhy*insnsntnsnss
cy*sc*ttkrkiitsts*krkrn*xk*kri*rifiqfngifnysrcsirslqnhlrrphw
cfiifistiilrwfr*ylqlwchrfsfrswyffnfihq*nskitsfkgtkikiw*qkiny

Frame B:
glleiiipphtiykftyi*KNTNTNKKFKMAGNKKQVKKNTKPIVVDIDDKPLLDTSNLD
TAVQTSASTKKDGKKVTATTTTTTPTPTPTPTPTPTTTTTTEKKSKEIEIXPTPSNVPAS
IXASTSNVXIAPAXEAQXTXDFXXXPXKNXX---

---XXXXXXXXXXXXXXXXSXXXXXXSXXXXXVXPTPXTTTQRTTTTKSTPTPTPTPTPA
ATKVVEQPKEKSSPAPVKKEKEIXQNKKESEGFLFSLMESLITPGVPSVVYKIIYVALIG
VLLFSLVPLYYAGLDSIYSYGVIALVLGLGISLTLFISEIPRLQASKEQKSKSGNKKST

Frame C:
aywkllypltlyinlhiykktqtqiknlkwqetrnklkrtlnqsllilminhy*ilqi*i
lqfkqvhqlkktekklllppppppqlqlqpqhqlqqllpqqkkkvkklklxqhhqmyqlq
xqhqlqmlxlhqlxkhkxlxtxqxxxxkxxx---

---xxxxxxxxxxxmxxxxxxxxxxxxxxlxxlxqlxqqplkeqpllnqlqlqhqlqlql
llkllnnqkknhhqhqlkkkkklxkikknlkdfysv*wnl*llqvfhp*ftksftspslv
fyyfh*yhyitlv*ivstvmvssl*f*vlvfl*lyssvkfqdyklqrnknqnlvtknql
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO150 (AFO150Q) /CSM/AF/AFO1-C/AFO150Q.Seq.d/                        545   e-154
SLE566 (SLE566Q) /CSM/SL/SLE5-C/SLE566Q.Seq.d/                        537   e-151
SLC662 (SLC662Q) /CSM/SL/SLC6-C/SLC662Q.Seq.d/                        537   e-151
SLB648 (SLB648Q) /CSM/SL/SLB6-B/SLB648Q.Seq.d/                        537   e-151
SLB638 (SLB638Q) /CSM/SL/SLB6-B/SLB638Q.Seq.d/                        537   e-151
SLA475 (SLA475Q) /CSM/SL/SLA4-D/SLA475Q.Seq.d/                        537   e-151
SFJ228 (SFJ228Q) /CSM/SF/SFJ2-B/SFJ228Q.Seq.d/                        537   e-151
SFI764 (SFI764Q) /CSM/SF/SFI7-C/SFI764Q.Seq.d/                        537   e-151
SFG629 (SFG629Q) /CSM/SF/SFG6-B/SFG629Q.Seq.d/                        537   e-151
SFE114 (SFE114Q) /CSM/SF/SFE1-A/SFE114Q.Seq.d/                        537   e-151
own update 2004. 4.27
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BE651335|BE651335.1 UI-M-BH3-asz-d-01-0-UI.r1 NIH_BMAP_M_S4 Mus musculus cDNA clone UI-M-BH3-asz-d-01-0-UI 5', mRNA sequence.    62  1e-05  1
BU604682|BU604682.1 AGENCOURT_10050672 NIH_MGC_144 Mus musculus cDNA clone IMAGE:6535829 5', mRNA sequence.    62  1e-05  1
BQ747766|BQ747766.1 UI-M-FA0-bxr-d-02-0-UI.r1 NIH_BMAP_FA0 Mus musculus cDNA clone IMAGE:5711953 5', mRNA sequence.    62  1e-05  1
BE688495|BE688495.1 uw46c08.x1 Soares_mammary_gland_NMLMG Mus musculus cDNA clone IMAGE:3465038 3', mRNA sequence.    62  1e-05  1
BE687711|BE687711.1 uw46c08.y1 Soares_mammary_gland_NMLMG Mus musculus cDNA clone IMAGE:3465038 5', mRNA sequence.    62  1e-05  1
BB271873|BB271873.2 Mus musculus 10 days neonate cortex cDNA, RIKEN full-length enriched library, clone:A830059M14, 3' end partial sequence.    62  1e-05  1
AL672276|AL672276.9 Mouse DNA sequence from clone RP23-124L1 on chromosome 4.    62  1e-05  1
AW495180|AW495180.1 UI-M-BH3-auw-d-07-0-UI.s1 NIH_BMAP_M_S4 Mus musculus cDNA clone UI-M-BH3-auw-d-07-0-UI 3', mRNA sequence.    62  1e-05  1
AW490807|AW490807.1 UI-M-BH3-asz-d-01-0-UI.s1 NIH_BMAP_M_S4 Mus musculus cDNA clone UI-M-BH3-asz-d-01-0-UI 3', mRNA sequence.    62  1e-05  1
BB641856|BB641856.1 Mus musculus 10 days neonate cortex cDNA, RIKEN full-length enriched library, clone:A830059M14, 5' end partial sequence.    62  1e-05  1
dna update 2003. 7.17
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54SG3) RecName: Full=Uncharacterized transmembrane protein DDB...   196   1e-48
AL136535_2(AL136535|pid:none) S.pombe chromosome II p1 p23A10.  ...    39   0.34
AP009179_1281(AP009179|pid:none) Sulfurovum sp. NBC37-1 genomic ...    37   0.77
AM295250_1660(AM295250|pid:none) Staphylococcus carnosus subsp. ...    36   2.2
AP008934_718(AP008934|pid:none) Staphylococcus saprophyticus sub...    34   8.5
AJ938182_2050(AJ938182|pid:none) Staphylococcus aureus RF122 com...    34   8.5
AB078343_3(AB078343|pid:none) Staphylococcus aureus genes for Se...    34   8.5
BA000033_2095(BA000033|pid:none) Staphylococcus aureus subsp. au...    34   8.5
EU490746_1(EU490746|pid:none) Gomphichis sp. Trujillo 379 hypoth...    34   8.5
AP009351_2080(AP009351|pid:none) Staphylococcus aureus subsp. au...    34   8.5
protein update 2009. 4.11
PSORT 

 psg: 0.57  gvh: 0.42  alm: 0.18  top: 0.47  tms: 0.13  mit: 0.30  mip: 0.00
 nuc: 0.11  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 40.0 %: endoplasmic reticulum
	 20.0 %: plasma membrane
	 16.0 %: mitochondrial
	  8.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal
	  4.0 %: cytoplasmic

>> prediction for AFO150 is end
5' end seq. ID AFO150F
5' end seq. 
>AFO150F.Seq
TGGCCTACTGGAAATTATTATACCCCCTCACACTATATATAAATTTACATATATATAAAA
AAACACAAACACAAATAAAAAATTTAAAATGGCAGGAAACAAGAAACAAGTTAAAAAGAA
CACTAAACCAATCGTTGTTGATATTGATGATAAACCATTATTAGATACTTCAAATTTAGA
TACTGCAGTTCAAACAAGTGCATCAACTAAAAAAGACGGAAAAAAAGTTACTGCTACCAC
CACCACCACCACCCCAACTCCAACTCCAACCCCAACACCAACTCCAACAACTACTACCAC
AACAGAAAAAAAAAGTAAAGAAATTGAAATTGNACCAACACCATCAAATGTACCAGCTTC
AATTNCAGCATCAACTTCAAATGTTGNAATTGCACCAGCTGNNGAAGCACAAATNACTGN
TGACTTTNCAGNNGNNCCANANAAAAATNNNNNNA----------
Length of 5' end seq. 455
3' end seq. ID AFO150Z
3' end seq. 
>AFO150Z.Seq
----------TNNNNNNTNNTNNNNNNNNNNNCNNNNNNNNNNNNATGNNTNNTNATNNT
CNTNATNTANNNNNTNNNNATCNNAANNNNCTNNNANAGTTGNNCCAACTCCANCAACAA
CCACTCAAAGAACAACCACTACTAAATCAACTCCAACTCCAACACCAACTCCAACTCCAG
CTGCTACTAAAGTTGTTGAACAACCAAAAGAAAAATCATCACCAGCACCAGTTAAAAAAG
AAAAAGAAATTAANCAAAATAAAAAAGAATCTGAAGGATTTTTATTCAGTTTAATGGAAT
CTTTAATTACTCCAGGTGTTCCATCCGTAGTTTACAAAATCATTTACGTCGCCCTCATTG
GTGTTTTATTATTTTCATTAGTACCATTATATTACGCTGGTTTAGATAGTATCTACAGTT
ATGGTGTCATCGCTTTAGTTTTAGGTCTTGGTATTTCTTTAACTTTATTCATCAGTGAAA
TTCCAAGATTACAAGCTTCAAAGGAACAAAAATCAAAATCTGGTAACAAAAAATCAACTA
C
Length of 3' end seq. 531
Connected seq. ID AFO150P
Connected seq. 
>AFO150P.Seq
TGGCCTACTGGAAATTATTATACCCCCTCACACTATATATAAATTTACATATATATAAAA
AAACACAAACACAAATAAAAAATTTAAAATGGCAGGAAACAAGAAACAAGTTAAAAAGAA
CACTAAACCAATCGTTGTTGATATTGATGATAAACCATTATTAGATACTTCAAATTTAGA
TACTGCAGTTCAAACAAGTGCATCAACTAAAAAAGACGGAAAAAAAGTTACTGCTACCAC
CACCACCACCACCCCAACTCCAACTCCAACCCCAACACCAACTCCAACAACTACTACCAC
AACAGAAAAAAAAAGTAAAGAAATTGAAATTGNACCAACACCATCAAATGTACCAGCTTC
AATTNCAGCATCAACTTCAAATGTTGNAATTGCACCAGCTGNNGAAGCACAAATNACTGN
TGACTTTNCAGNNGNNCCANANAAAAATNNNNNNA----------TNNNNNNTNNTNNNN
NNNNNNNCNNNNNNNNNNNNATGNNTNNTNATNNTCNTNATNTANNNNNTNNNNATCNNA
ANNNNCTNNNANAGTTGNNCCAACTCCANCAACAACCACTCAAAGAACAACCACTACTAA
ATCAACTCCAACTCCAACACCAACTCCAACTCCAGCTGCTACTAAAGTTGTTGAACAACC
AAAAGAAAAATCATCACCAGCACCAGTTAAAAAAGAAAAAGAAATTAANCAAAATAAAAA
AGAATCTGAAGGATTTTTATTCAGTTTAATGGAATCTTTAATTACTCCAGGTGTTCCATC
CGTAGTTTACAAAATCATTTACGTCGCCCTCATTGGTGTTTTATTATTTTCATTAGTACC
ATTATATTACGCTGGTTTAGATAGTATCTACAGTTATGGTGTCATCGCTTTAGTTTTAGG
TCTTGGTATTTCTTTAACTTTATTCATCAGTGAAATTCCAAGATTACAAGCTTCAAAGGA
ACAAAAATCAAAATCTGGTAACAAAAAATCAACTAC
Length of connected seq. 986
Full length Seq ID -
Full length Seq. -
Length of full length seq. -