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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO146
Library AF
(Link to library)
Clone ID AFO146
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16381-1
Original site URL
Representative seq. ID AFO146P
(Link to Original site)
Representative DNA sequence 
>AFO146 (AFO146Q) /CSM/AF/AFO1-B/AFO146Q.Seq.d/
ACTGTTGGCCTACTGGNATTGTAGTATTTTGTTCATATTCAAATTAAATATCAATACAAT
TAAAAAAAGTTAAAATTAAATTTGTAAAATCAATTTGTAACAAAAATTAGTAATTTAAAA
AAAAAAAAACCAAAAAAAAAAXXXXXXXXXXTGTCGAATGTCCAGATTTCCATANATGTG
TTGAAAAACGNGGTGGNATNTTAAGTTGTGAATTTGATCCNCCAAGACAACCAAGATCCC
TTGATTGGGCTGAAAATGAAAATGATGATCGTGACTATGATGATCGTGACTATGATGATG
ATGAATATGATGGTGATTATGATGGCCGTGACGGTGACTATGATGGTGATTATGATGGNG
ATTATGATGATGNCNATTATTATGGTGAT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
tvgllxl*yfvhiqikyqyn*kklklnl*NQFVTKISNLKKKKPKKK---

---VECPDFHXCVEKRGGXLSCEFDPPRQPRSLDWAENENDDRDYDDRDYDDDEYDGDYD
GRDGDYDGDYDGDYDDXXYYGD
Translated Amino Acid sequence (All Frames) 
Frame A:
tvgllxl*yfvhiqikyqyn*kklklnl*NQFVTKISNLKKKKPKKK---

---crmsrfpxmc*ktxwxxkl*i*sxkttkip*lg*k*k**s*l**s*l****i*w*l*
wp*r*l*w*l*wxl**xxllw*

Frame B:
llaywxcsilfifklnintikks*n*ickinl*qklvi*kkknqkk---

---VECPDFHXCVEKRGGXLSCEFDPPRQPRSLDWAENENDDRDYDDRDYDDDEYDGDYD
GRDGDYDGDYDGDYDDXXYYGD

Frame C:
cwptgivvfcsysn*isiqlkkvkikfvksicnkn**fkkkktkkk---

---snvqisixvlknxvxx*vvnlixqdnqdpliglkmkmmivtmmivtmmmmnmmvimm
avtvtmmvimmximmmxiimv
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO146 (AFO146Q) /CSM/AF/AFO1-B/AFO146Q.Seq.d/                        182   3e-45
SFI266 (SFI266Q) /CSM/SF/SFI2-C/SFI266Q.Seq.d/                        174   6e-43
SFI246 (SFI246Q) /CSM/SF/SFI2-B/SFI246Q.Seq.d/                        174   6e-43
SFI173 (SFI173Q) /CSM/SF/SFI1-D/SFI173Q.Seq.d/                        174   6e-43
SFH550 (SFH550Q) /CSM/SF/SFH5-C/SFH550Q.Seq.d/                        174   6e-43
SFE754 (SFE754Q) /CSM/SF/SFE7-C/SFE754Q.Seq.d/                        174   6e-43
SFE673 (SFE673Q) /CSM/SF/SFE6-D/SFE673Q.Seq.d/                        174   6e-43
SFE279 (SFE279Q) /CSM/SF/SFE2-D/SFE279Q.Seq.d/                        174   6e-43
SFC448 (SFC448Q) /CSM/SF/SFC4-B/SFC448Q.Seq.d/                        174   6e-43
SFC196 (SFC196Q) /CSM/SF/SFC1-D/SFC196Q.Seq.d/                        174   6e-43
own update 2004. 4.27
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X51892|X51892.1 Dictyostelium discoideum SP60 gene for spore coat protein.   159  2e-94  4
AC115685|AC115685.1 Dictyostelium discoideum chromosome 2 map 4718821-4752388 strain AX4, complete sequence.   159  4e-91  4
AC116977|AC116977.2 Dictyostelium discoideum chromosome 2 map 5515173-5817331 strain AX4, complete sequence.   159  3e-88  4
M26239|M26239.1 D.discoideum spore coat protein SP60 gene, complete cds.   159  1e-75  3
X52105|X52105.1 Dictyostelium discoideum SP60 gene for spore coat protein.    80  2e-25  2
M34546|M34546.1 D.discoideum spore coat 60 (sp60) gene, 5' flank.    88  9e-14  1
BM167254|BM167254.1 EST569777 PyBS Plasmodium yoelii yoelii cDNA clone PYCOG24 5' end, mRNA sequence.    42  2e-04  2
AF078801|AF078801.1 Hordeum vulgare degenerate retrotransposon cereba polyprotein pseudogene, partial sequence.    54  0.001  1
BV025443|BV025443.1 S212P6456FC5.T0 CZECHII/Ei Mus musculus STS genomic, sequence tagged site.    42  0.16  2
AC116612|AC116612.5 Homo sapiens BAC clone RP13-582L3 from 4, complete sequence.    40  0.31  2
dna update 2003. 9.12
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P15270) RecName: Full=Spore coat protein SP60; Flags: Precursor...    82   6e-15
protein update 2008.11.27
PSORT 

 psg: 0.23  gvh: 0.34  alm: 0.64  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.00
 nuc: 0.22  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: nuclear
	 28.0 %: cytoplasmic
	  8.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: plasma membrane

>> prediction for AFO146 is nuc
5' end seq. ID AFO146F
5' end seq. 
>AFO146F.Seq
ACTGTTGGCCTACTGGNATTGTAGTATTTTGTTCATATTCAAATTAAATATCAATACAAT
TAAAAAAAGTTAAAATTAAATTTGTAAAATCAATTTGTAACAAAAATTAGTAATTTAAAA
AAAAAAAAACCAAAAAAAAAA----------
Length of 5' end seq. 141
3' end seq. ID AFO146Z
3' end seq. 
>AFO146Z.Seq
----------TGTCGAATGTCCAGATTTCCATANATGTGTTGAAAAACGNGGTGGNATNT
TAAGTTGTGAATTTGATCCNCCAAGACAACCAAGATCCCTTGATTGGGCTGAAAATGAAA
ATGATGATCGTGACTATGATGATCGTGACTATGATGATGATGAATATGATGGTGATTATG
ATGGCCGTGACGGTGACTATGATGGTGATTATGATGGNGATTATGATGATGNCNATTATT
ATGGTGAT
Length of 3' end seq. 238
Connected seq. ID AFO146P
Connected seq. 
>AFO146P.Seq
ACTGTTGGCCTACTGGNATTGTAGTATTTTGTTCATATTCAAATTAAATATCAATACAAT
TAAAAAAAGTTAAAATTAAATTTGTAAAATCAATTTGTAACAAAAATTAGTAATTTAAAA
AAAAAAAAACCAAAAAAAAAA----------TGTCGAATGTCCAGATTTCCATANATGTG
TTGAAAAACGNGGTGGNATNTTAAGTTGTGAATTTGATCCNCCAAGACAACCAAGATCCC
TTGATTGGGCTGAAAATGAAAATGATGATCGTGACTATGATGATCGTGACTATGATGATG
ATGAATATGATGGTGATTATGATGGCCGTGACGGTGACTATGATGGTGATTATGATGGNG
ATTATGATGATGNCNATTATTATGGTGAT
Length of connected seq. 379
Full length Seq ID -
Full length Seq. -
Length of full length seq. -