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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHH281
Library VH
(Link to library)
Clone ID VHH281
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U16208-1
Original site URL
Representative seq. ID VHH281P
(Link to Original site)
Representative DNA sequence 
>VHH281 (VHH281Q) /CSM/VH/VHH2-D/VHH281Q.Seq.d/
AAAAAACATTACAATTTAAAATAATATTTAATTATGAGTGAAACAACACCAACAAATGTT
ATTACAAGTTTATCAATTGAAGAAACGAATAGGATCAGAATTTCATTAGGTTTAAAACCA
TTAAAAGAAGAGTCAAAAGAAGTAAAACCACAAACAACAACAAATAATAATAATAATAAT
AATAATACAAATAGTGATACTTTAGAAGTATTGGATACAATTAATAGATTAAAAAAAAAA
AGAGAATTAAATTCAAAATTAGCAGGTAAATCTATAGCTGANCAATTATTANAAGGNGAA
AATGAAGATGAAGATGATGATNTATCAAGTTGGATAAATAAATCAAGACTAAATGAAAAA
CAAAANCAAATTGAAAGAAAGAAAGAATTNTTAAATAAAAAACANCAAAANCAXXXXXXX
XXXTACAACAAATAATAATAAAATTGATGATAATACAGTAACTTCATTATCAAATGAAAA
TAATAATAATAATAATGATAGTGATGATAATAATAATAATAGTAATAATAATAATAAAGA
TGAAGAAATGAAAAATATTGACGATGGCGATGATGATGATGACGAAAATGAAGAAGATGA
AGATTATAATAAATATAAAGAAAAAGAAAAGAAATATAATAATTTATCAGCAATATTACC
AGAGGAACCATTAGTATCAGGTAGTGTTTCAGCAGCATTAAGATTATTTGCACAAAAAGG
TGATTTACAACCAAATCCAGTTACAAACAATAGGAAAAGAGGTATTAATTATGATAATGA
TGAATCTACTGTAATTGAACATAAAGATGAATTTGGTAGAGTTATGAGTAGAAAAGAAGC
ATTTGTAAAGATGTCTCAAGTATTTCATGGTAAAAAATCTGGTAAAAATAAAATGGAGAA
AAGAAAAAGACTCTATGAGGAGGAATTAAAGAAAATGAAAATGGATTCAAACGATACTCC
ATTAGGTATGGTTAAATCATTCCAATCCTATCAAGAGAAAACAAATTCACCATATTTAGT
TTTATCAGGTGGTGGTGGTGCTGCAAATATTTTAAAACAAAATATTAATAATATCAAACT
ACAAATAAAAAATAAATTAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
kkhynlk*YLIMSETTPTNVITSLSIEETNRIRISLGLKPLKEESKEVKPQTTTNNNNNN
NNTNSDTLEVLDTINRLKKKRELNSKLAGKSIAXQLLXGENEDEDDDXSSWINKSRLNEK
QXQIERKKEXLNKKXQX---

---TTNNNKIDDNTVTSLSNENNNNNNDSDDNNNNSNNNNKDEEMKNIDDGDDDDDENEE
DEDYNKYKEKEKKYNNLSAILPEEPLVSGSVSAALRLFAQKGDLQPNPVTNNRKRGINYD
NDESTVIEHKDEFGRVMSRKEAFVKMSQVFHGKKSGKNKMEKRKRLYEEELKKMKMDSND
TPLGMVKSFQSYQEKTNSPYLVLSGGGGAANILKQNINNIKLQIKNKLK
Translated Amino Acid sequence (All Frames) 
Frame A:
kkhynlk*YLIMSETTPTNVITSLSIEETNRIRISLGLKPLKEESKEVKPQTTTNNNNNN
NNTNSDTLEVLDTINRLKKKRELNSKLAGKSIAXQLLXGENEDEDDDXSSWINKSRLNEK
QXQIERKKEXLNKKXQX---

---ynk***n***ysnfiik*k*******************r*rneky*rwr****rk*r
r*rl**i*rkrkei**fisnitrgtisir*cfssikiictkr*fttkssykq*ekry*l*
***iycn*t*r*iw*sye*krsickdvssisw*kiw*k*ngekkktl*ggikenengfkr
ysiryg*iipilsrenkftifsfirwwwcckyfktky**yqttnkk*ik

Frame B:
kniti*nni*l*vkqhqqmllqvyqlkkrigsefh*v*nh*kksqkk*nhkqqqiiiiii
iiqivil*kywiqlid*kkken*iqn*qvnl*lxnyxkxkmkmkmmxyqvg*inqd*mkn
kxklkerknx*iknxkx---

---TTNNNKIDDNTVTSLSNENNNNNNDSDDNNNNSNNNNKDEEMKNIDDGDDDDDENEE
DEDYNKYKEKEKKYNNLSAILPEEPLVSGSVSAALRLFAQKGDLQPNPVTNNRKRGINYD
NDESTVIEHKDEFGRVMSRKEAFVKMSQVFHGKKSGKNKMEKRKRLYEEELKKMKMDSND
TPLGMVKSFQSYQEKTNSPYLVLSGGGGAANILKQNINNIKLQIKNKLK

Frame C:
ktlqfkiifnye*nntnkcyykfin*rne*dqnfirfktikrrvkrskttnnnk******
*yk**yfrsigyn**ikkkkrikfkisr*iys*xiixrxk*r*r**xikldk*iktk*kt
kxn*kkerixk*ktxkx---

---qqiiiklmiiq*lhyqmkiiiiimivmiiiiiviiiikmkk*kiltmammmmtkmkk
mkiiinikkkkrniiiyqqyyqrnh*yqvvfqqh*dylhkkviynqiqlqtigkevlimi
mmnll*lnikmnlvel*vekkhl*rclkyfmvknlvkikwrkekdsmrrn*rk*kwiqti
lh*vwlnhsnpikrkqihhi*fyqvvvvlqif*nkiliisnyk*kin*k
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHH281 (VHH281Q) /CSM/VH/VHH2-D/VHH281Q.Seq.d/                        450   e-125
VSC863 (VSC863Q) /CSM/VS/VSC8-C/VSC863Q.Seq.d/                        165   2e-39
VSB834 (VSB834Q) /CSM/VS/VSB8-B/VSB834Q.Seq.d/                        109   9e-23
SSM246 (SSM246Q) /CSM/SS/SSM2-B/SSM246Q.Seq.d/                         50   7e-05
VHP325 (VHP325Q) /CSM/VH/VHP3-B/VHP325Q.Seq.d/                         48   3e-04
VHO501 (VHO501Q) /CSM/VH/VHO5-A/VHO501Q.Seq.d/                         48   3e-04
VHL758 (VHL758Q) /CSM/VH/VHL7-C/VHL758Q.Seq.d/                         48   3e-04
SFI587 (SFI587Q) /CSM/SF/SFI5-D/SFI587Q.Seq.d/                         48   3e-04
AFB383 (AFB383Q) /CSM/AF/AFB3-D/AFB383Q.Seq.d/                         48   3e-04
AFB319 (AFB319Q) /CSM/AF/AFB3-A/AFB319Q.Seq.d/                         48   3e-04
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ441121) Dictyostelium discoideum cDNA clone:ddv45a22, 3' ...   452   e-169  2
(BJ422381) Dictyostelium discoideum cDNA clone:ddv45a22, 5' ...   321   e-145  3
(AU262845) Dictyostelium discoideum vegetative cDNA clone:VS...   161   2e-44  3
(AU262279) Dictyostelium discoideum vegetative cDNA clone:VS...   105   4e-18  1
(DN907062) 21813.3 Developing Tubers Solanum tuberosum cDNA ...    48   0.041  2
(AC114263) Dictyostelium discoideum chromosome 2 map 215673-...    40   0.27   6
(DR924738) EST1116277 Aquilegia cDNA library Aquilegia formo...    38   0.55   2
(DT758368) EST1192217 Aquilegia cDNA library Aquilegia formo...    38   0.57   2
(CZ321028) ZMMBF0023A17r ZMMBF Zea mays genomic clone ZMMBF0...    38   0.69   2
(DR917799) EST1109338 Aquilegia cDNA library Aquilegia formo...    38   0.70   2
dna update 2008.11. 7
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC076544_1(BC076544|pid:none) Danio rerio squamous cell carcinom...    69   2e-10
AK223016_1(AK223016|pid:none) Homo sapiens mRNA for squamous cel...    68   7e-10
AF299060_1(AF299060|pid:none) Tetraodon nigroviridis squamous-ce...    66   2e-09
AF042797_4(AF042797|pid:none) Takifugu rubripes FRG1 (FRG1), N-a...    66   2e-09
AF129931_1(AF129931|pid:none) Mus musculus hypoxia associated fa...    65   3e-09
(Q5XIW8) RecName: Full=U4/U6.U5 tri-snRNP-associated protein 1; ...    65   3e-09
BC028823_1(BC028823|pid:none) Mus musculus squamous cell carcino...    65   3e-09
(Q9Z315) RecName: Full=U4/U6.U5 tri-snRNP-associated protein 1; ...    65   3e-09
BC043853_1(BC043853|pid:none) Xenopus laevis similar to squamous...    65   4e-09
AF292118_1(AF292118|pid:none) Takifugu rubripes clone 1-h10 squa...    64   9e-09
protein update 2009. 7.12
PSORT 

 psg: 0.75  gvh: 0.42  alm: 0.45  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.08  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: vacuolar
	  4.0 %: endoplasmic reticulum
	  4.0 %: peroxisomal

>> prediction for VHH281 is cyt
5' end seq. ID VHH281F
5' end seq. 
>VHH281F.Seq
AAAAAACATTACAATTTAAAATAATATTTAATTATGAGTGAAACAACACCAACAAATGTT
ATTACAAGTTTATCAATTGAAGAAACGAATAGGATCAGAATTTCATTAGGTTTAAAACCA
TTAAAAGAAGAGTCAAAAGAAGTAAAACCACAAACAACAACAAATAATAATAATAATAAT
AATAATACAAATAGTGATACTTTAGAAGTATTGGATACAATTAATAGATTAAAAAAAAAA
AGAGAATTAAATTCAAAATTAGCAGGTAAATCTATAGCTGANCAATTATTANAAGGNGAA
AATGAAGATGAAGATGATGATNTATCAAGTTGGATAAATAAATCAAGACTAAATGAAAAA
CAAAANCAAATTGAAAGAAAGAAAGAATTNTTAAATAAAAAACANCAAAANCANNNNNNN
NNN
Length of 5' end seq. 423
3' end seq. ID VHH281Z
3' end seq. 
>VHH281Z.Seq
NNNNNNNNNNTACAACAAATAATAATAAAATTGATGATAATACAGTAACTTCATTATCAA
ATGAAAATAATAATAATAATAATGATAGTGATGATAATAATAATAATAGTAATAATAATA
ATAAAGATGAAGAAATGAAAAATATTGACGATGGCGATGATGATGATGACGAAAATGAAG
AAGATGAAGATTATAATAAATATAAAGAAAAAGAAAAGAAATATAATAATTTATCAGCAA
TATTACCAGAGGAACCATTAGTATCAGGTAGTGTTTCAGCAGCATTAAGATTATTTGCAC
AAAAAGGTGATTTACAACCAAATCCAGTTACAAACAATAGGAAAAGAGGTATTAATTATG
ATAATGATGAATCTACTGTAATTGAACATAAAGATGAATTTGGTAGAGTTATGAGTAGAA
AAGAAGCATTTGTAAAGATGTCTCAAGTATTTCATGGTAAAAAATCTGGTAAAAATAAAA
TGGAGAAAAGAAAAAGACTCTATGAGGAGGAATTAAAGAAAATGAAAATGGATTCAAACG
ATACTCCATTAGGTATGGTTAAATCATTCCAATCCTATCAAGAGAAAACAAATTCACCAT
ATTTAGTTTTATCAGGTGGTGGTGGTGCTGCAAATATTTTAAAACAAAATATTAATAATA
TCAAACTACAAATAAAAAATAAATTAAAAA
Length of 3' end seq. 690
Connected seq. ID VHH281P
Connected seq. 
>VHH281P.Seq
AAAAAACATTACAATTTAAAATAATATTTAATTATGAGTGAAACAACACCAACAAATGTT
ATTACAAGTTTATCAATTGAAGAAACGAATAGGATCAGAATTTCATTAGGTTTAAAACCA
TTAAAAGAAGAGTCAAAAGAAGTAAAACCACAAACAACAACAAATAATAATAATAATAAT
AATAATACAAATAGTGATACTTTAGAAGTATTGGATACAATTAATAGATTAAAAAAAAAA
AGAGAATTAAATTCAAAATTAGCAGGTAAATCTATAGCTGANCAATTATTANAAGGNGAA
AATGAAGATGAAGATGATGATNTATCAAGTTGGATAAATAAATCAAGACTAAATGAAAAA
CAAAANCAAATTGAAAGAAAGAAAGAATTNTTAAATAAAAAACANCAAAANCA-------
---TACAACAAATAATAATAAAATTGATGATAATACAGTAACTTCATTATCAAATGAAAA
TAATAATAATAATAATGATAGTGATGATAATAATAATAATAGTAATAATAATAATAAAGA
TGAAGAAATGAAAAATATTGACGATGGCGATGATGATGATGACGAAAATGAAGAAGATGA
AGATTATAATAAATATAAAGAAAAAGAAAAGAAATATAATAATTTATCAGCAATATTACC
AGAGGAACCATTAGTATCAGGTAGTGTTTCAGCAGCATTAAGATTATTTGCACAAAAAGG
TGATTTACAACCAAATCCAGTTACAAACAATAGGAAAAGAGGTATTAATTATGATAATGA
TGAATCTACTGTAATTGAACATAAAGATGAATTTGGTAGAGTTATGAGTAGAAAAGAAGC
ATTTGTAAAGATGTCTCAAGTATTTCATGGTAAAAAATCTGGTAAAAATAAAATGGAGAA
AAGAAAAAGACTCTATGAGGAGGAATTAAAGAAAATGAAAATGGATTCAAACGATACTCC
ATTAGGTATGGTTAAATCATTCCAATCCTATCAAGAGAAAACAAATTCACCATATTTAGT
TTTATCAGGTGGTGGTGGTGCTGCAAATATTTTAAAACAAAATATTAATAATATCAAACT
ACAAATAAAAAATAAATTAAAAA
Length of connected seq. 1093
Full length Seq ID -
Full length Seq. -
Length of full length seq. -