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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHH274
Library VH
(Link to library)
Clone ID VHH274
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11369-1
Original site URL
Representative seq. ID VHH274P
(Link to Original site)
Representative DNA sequence 
>VHH274 (VHH274Q) /CSM/VH/VHH2-D/VHH274Q.Seq.d/
ATTATAATAATTTTTTAATTGCTTTTTAATTGTTTATTGTTTATTTTTTATTTTTTAATT
AATTATTTCATAATTTCATAATTTTTTAATTTTTTTAAAAAAAAAAAGCAAAAAGCAAAA
AAGCAAAGATTTGGTNTAATAAAAAAAAAANGANAAAAAGAACCNAAAAAAAAAXXXXXX
XXXXCCTTGGTTTAAGAATTCAACGTATGCCTTCCGATTCAAATAAAGATTTCTATCATC
CAAATGAATATTCCTATCTCACTGTAAATACTACCTCATCTCATGATATGAGTACCTTGA
GAGGTTGGTGGGAAGAAGATAGAGCTCGTTCTCAAGTTTTCTACAATAAAATCTTGGGAA
TGTTTGGTGATGCTCCTTACTTTTGTGAACCTTATGTTAGTGAGGCCGTTATCACTCAAC
ATTTACATTCTCAATCTATGATTTCAA
sequence update 2002.10.25
Translated Amino Acid sequence 
iiiif*llfncllfifyflinyfiis*FFNFFKKKKQKAKKQRFGXIKKKXXKEPKKK--
-

---LGLRIQRMPSDSNKDFYHPNEYSYLTVNTTSSHDMSTLRGWWEEDRARSQVFYNKIL
GMFGDAPYFCEPYVSEAVITQHLHSQSMIS
Translated Amino Acid sequence (All Frames) 
Frame A:
iiiif*llfncllfifyflinyfiis*FFNFFKKKKQKAKKQRFGXIKKKXXKEPKKK--
-

---pwfknstyafrfk*rflssk*iflshckyylis*yeylerlvgrr*ssfssflq*nl
gnvw*cslll*tlc**gryhstftfsiydf

Frame B:
l**ffncflivyclffif*liis*fhnfliflkkkskkqkskdlv**kkxxkknxkk---

---LGLRIQRMPSDSNKDFYHPNEYSYLTVNTTSSHDMSTLRGWWEEDRARSQVFYNKIL
GMFGDAPYFCEPYVSEAVITQHLHSQSMIS

Frame C:
ynnfliaf*lfivyflffn*lfhnfiif*ff*kkkakskkakiwxnkkkxxkrtxkk---

---lv*efnvclpiqikisiiqmnipisl*ilphlmi*vp*evggkkielvlkfstiksw
eclvmlltfvnlmlvrplslniyilnl*fq
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ322 (VHQ322Q) /CSM/VH/VHQ3-A/VHQ322Q.Seq.d/                        521   e-147
VHN809 (VHN809Q) /CSM/VH/VHN8-A/VHN809Q.Seq.d/                        521   e-147
VHM715 (VHM715Q) /CSM/VH/VHM7-A/VHM715Q.Seq.d/                        521   e-147
VHM111 (VHM111Q) /CSM/VH/VHM1-A/VHM111Q.Seq.d/                        521   e-147
VHL717 (VHL717Q) /CSM/VH/VHL7-A/VHL717Q.Seq.d/                        521   e-147
VHL374 (VHL374Q) /CSM/VH/VHL3-D/VHL374Q.Seq.d/                        521   e-147
VHK364 (VHK364Q) /CSM/VH/VHK3-C/VHK364Q.Seq.d/                        521   e-147
VHK286 (VHK286Q) /CSM/VH/VHK2-D/VHK286Q.Seq.d/                        521   e-147
VHH879 (VHH879Q) /CSM/VH/VHH8-D/VHH879Q.Seq.d/                        521   e-147
VHH274 (VHH274Q) /CSM/VH/VHH2-D/VHH274Q.Seq.d/                        521   e-147
own update 2002.12.29
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ446577) Dictyostelium discoideum cDNA clone:ddv63l05, 3' ...   521   e-144  1
(BJ444958) Dictyostelium discoideum cDNA clone:ddv58b04, 3' ...   521   e-144  1
(BJ444376) Dictyostelium discoideum cDNA clone:ddv56n03, 3' ...   521   e-144  1
(BJ444046) Dictyostelium discoideum cDNA clone:ddv55e03, 3' ...   521   e-144  1
(BJ443752) Dictyostelium discoideum cDNA clone:ddv54b05, 3' ...   521   e-144  1
(BJ443686) Dictyostelium discoideum cDNA clone:ddv53d19, 3' ...   521   e-144  1
(BJ443093) Dictyostelium discoideum cDNA clone:ddv51k22, 3' ...   521   e-144  1
(BJ443037) Dictyostelium discoideum cDNA clone:ddv51p15, 3' ...   521   e-144  1
(BJ441511) Dictyostelium discoideum cDNA clone:ddv46n20, 3' ...   521   e-144  1
(BJ441130) Dictyostelium discoideum cDNA clone:ddv45c20, 3' ...   521   e-144  1
dna update 2008.11. 7
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AP006841_3821(AP006841|pid:none) Bacteroides fragilis YCH46 DNA,...    94   2e-18
CR626927_3520(CR626927|pid:none) Bacteroides fragilis NCTC 9343,...    93   3e-18
BT055804_1(BT055804|pid:none) Zea mays full-length cDNA clone ZM...    89   4e-17
AY081315_1(AY081315|pid:none) Arabidopsis thaliana 4-alpha-gluca...    88   8e-17
FJ664262_1(FJ664262|pid:none) Annona cherimola disproportionatin...    87   1e-16
AE005176_696(AE005176|pid:none) Lactococcus lactis subsp. lactis...    85   9e-16
CP000139_1346(CP000139|pid:none) Bacteroides vulgatus ATCC 8482,...    84   1e-15
AP010656_444(AP010656|pid:none) Candidatus Azobacteroides pseudo...    84   2e-15
AY510449_1(AY510449|pid:none) Solanum tuberosum 4-alpha-glucanot...    82   5e-15
CP000140_3(CP000140|pid:none) Parabacteroides distasonis ATCC 85...    75   6e-13
protein update 2009. 7.12
PSORT 

 psg: 0.47  gvh: 0.20  alm: 0.50  top: 0.53  tms: 0.00  mit: 0.34  mip: 0.07
 nuc: 0.25  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 80.0 %: nuclear
	 16.0 %: cytoplasmic
	  4.0 %: plasma membrane

>> prediction for VHH274 is nuc
5' end seq. ID VHH274F
5' end seq. 
>VHH274F.Seq
ATTATAATAATTTTTTAATTGCTTTTTAATTGTTTATTGTTTATTTTTTATTTTTTAATT
AATTATTTCATAATTTCATAATTTTTTAATTTTTTTAAAAAAAAAAAGCAAAAAGCAAAA
AAGCAAAGATTTGGTNTAATAAAAAAAAAANGANAAAAAGAACCNAAAAAAAAANNNNNN
NNNN
Length of 5' end seq. 184
3' end seq. ID VHH274Z
3' end seq. 
>VHH274Z.Seq
NNNNNNNNNNCCTTGGTTTAAGAATTCAACGTATGCCTTCCGATTCAAATAAAGATTTCT
ATCATCCAAATGAATATTCCTATCTCACTGTAAATACTACCTCATCTCATGATATGAGTA
CCTTGAGAGGTTGGTGGGAAGAAGATAGAGCTCGTTCTCAAGTTTTCTACAATAAAATCT
TGGGAATGTTTGGTGATGCTCCTTACTTTTGTGAACCTTATGTTAGTGAGGCCGTTATCA
CTCAACATTTACATTCTCAATCTATGATTTCAA
Length of 3' end seq. 273
Connected seq. ID VHH274P
Connected seq. 
>VHH274P.Seq
ATTATAATAATTTTTTAATTGCTTTTTAATTGTTTATTGTTTATTTTTTATTTTTTAATT
AATTATTTCATAATTTCATAATTTTTTAATTTTTTTAAAAAAAAAAAGCAAAAAGCAAAA
AAGCAAAGATTTGGTNTAATAAAAAAAAAANGANAAAAAGAACCNAAAAAAAAA------
----CCTTGGTTTAAGAATTCAACGTATGCCTTCCGATTCAAATAAAGATTTCTATCATC
CAAATGAATATTCCTATCTCACTGTAAATACTACCTCATCTCATGATATGAGTACCTTGA
GAGGTTGGTGGGAAGAAGATAGAGCTCGTTCTCAAGTTTTCTACAATAAAATCTTGGGAA
TGTTTGGTGATGCTCCTTACTTTTGTGAACCTTATGTTAGTGAGGCCGTTATCACTCAAC
ATTTACATTCTCAATCTATGATTTCAA
Length of connected seq. 437
Full length Seq ID -
Full length Seq. -
Length of full length seq. -