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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHH270
Library VH
(Link to library)
Clone ID VHH270
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15685-1
Original site URL
Representative seq. ID VHH270P
(Link to Original site)
Representative DNA sequence 
>VHH270 (VHH270Q) /CSM/VH/VHH2-C/VHH270Q.Seq.d/
AACTATTTAAAAAAAAATAGAGAATATAGTTATATATATAACACACAAAAAAATTCATAT
CACATTATTGTTTTTCATTAATTTATAAACAATAATATATATATAATAAACACTCTATCA
TTTAATTTTAATAAAAAAAAAAXXXXXXXXXXGTCAAGGTGCTGTTCAAACCGCTCTCTT
ATTCTTGGCTCTCATTTCCATTCCAGTTATGTTGGTCATTAAACCATTGTTCATGAAGAG
ATTCCACTTCCAAGAAGTCGAAAGAAAGAAATTAGGTCACCACGAAGAGGAACATGATGA
TGAAGCTCTCTACACTGGTCATCACGGTGAAGAATTTGAAATGGGTGAAGTCTTTGTTCA
TCAAGTCATTCATACCATCGAGTTTGTTTTGGGTGCTGTTTCAAATACTGCCTCTTATCT
TCGTTTATGGGCTCTTTCATTGGCTCACTCTGAGTTATCTTCTGTATTTTGGGAACGTAT
TCTCATTGGTCAAGTCGAACGTGGTAATCCATTCTTGGCTTTCGTTGGTTTTGGTGCTTG
NNTANGCTCCTCAGTGGATGT
sequence update 2002.10.25
Translated Amino Acid sequence 
nylkknreysyiyntqknsyhiivfh*FINNNIYIINTLSFNFNKKK---

---QGAVQTALLFLALISIPVMLVIKPLFMKRFHFQEVERKKLGHHEEEHDDEALYTGHH
GEEFEMGEVFVHQVIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFWERILIGQVERG
NPFLAFVGFGAXXXSSVD
Translated Amino Acid sequence (All Frames) 
Frame A:
nylkknreysyiyntqknsyhiivfh*FINNNIYIINTLSFNFNKKK---

---vkvlfkplsyswlsfpfqlcwslnhcs*rdstskkskern*vttkrnmmmklstlvi
tvknlkwvkslfiksfipsslfwvlfqilplifvyglfhwltlsyllyfgnvfslvksnv
vihswlslvlvlxxapqwm

Frame B:
ti*kkieniviyithkkihitllffinl*tiiyi**tlyhlilikkk---

---srccsnrslilgshfhssyvgh*tivheeiplprsrkkeirsprrgt***sslhwss
r*ri*ng*slcssshsyhrvcfgccfkycllssfmgsfigsl*vifcilgtyshwssrtw
*silgfrwfwclxxllsgc

Frame C:
lfkkk*ri*lyi*htkkfishycfsliykq*yiynkhsii*f**kk---

---QGAVQTALLFLALISIPVMLVIKPLFMKRFHFQEVERKKLGHHEEEHDDEALYTGHH
GEEFEMGEVFVHQVIHTIEFVLGAVSNTASYLRLWALSLAHSELSSVFWERILIGQVERG
NPFLAFVGFGAXXXSSVD
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHH270 (VHH270Q) /CSM/VH/VHH2-C/VHH270Q.Seq.d/                        793   0.0
VHQ150 (VHQ150Q) /CSM/VH/VHQ1-C/VHQ150Q.Seq.d/                        769   0.0
VHP711 (VHP711Q) /CSM/VH/VHP7-A/VHP711Q.Seq.d/                        769   0.0
VHN341 (VHN341Q) /CSM/VH/VHN3-B/VHN341Q.Seq.d/                        769   0.0
VHN293 (VHN293Q) /CSM/VH/VHN2-D/VHN293Q.Seq.d/                        769   0.0
VHL159 (VHL159Q) /CSM/VH/VHL1-C/VHL159Q.Seq.d/                        769   0.0
VHK808 (VHK808Q) /CSM/VH/VHK8-A/VHK808Q.Seq.d/                        769   0.0
VHI202 (VHI202Q) /CSM/VH/VHI2-A/VHI202Q.Seq.d/                        769   0.0
VHG103 (VHG103Q) /CSM/VH/VHG1-A/VHG103Q.Seq.d/                        769   0.0
VHC159 (VHC159Q) /CSM/VH/VHC1-C/VHC159Q.Seq.d/                        769   0.0
own update 2004. 5.26
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ441090) Dictyostelium discoideum cDNA clone:ddv45k18, 3' ...   791   0.0    1
(U38803) Dictyostelium discoideum vacuolar proton ATPase 100...   767   0.0    1
(BJ446681) Dictyostelium discoideum cDNA clone:ddv63c13, 3' ...   767   0.0    1
(BJ446226) Dictyostelium discoideum cDNA clone:ddv62f03, 3' ...   767   0.0    1
(BJ444917) Dictyostelium discoideum cDNA clone:ddv57i24, 3' ...   767   0.0    1
(BJ444719) Dictyostelium discoideum cDNA clone:ddv57b11, 3' ...   767   0.0    1
(BJ443621) Dictyostelium discoideum cDNA clone:ddv53e15, 3' ...   767   0.0    1
(BJ443202) Dictyostelium discoideum cDNA clone:ddv52p02, 3' ...   767   0.0    1
(BJ441538) Dictyostelium discoideum cDNA clone:ddv47c02, 3' ...   767   0.0    1
(BJ440226) Dictyostelium discoideum cDNA clone:ddv43e01, 3' ...   767   0.0    1
dna update 2008.11. 7
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54E04) RecName: Full=Vacuolar proton translocating ATPase 100 ...   261   7e-69
U38803_1(U38803|pid:none) Dictyostelium discoideum vacuolar prot...   261   7e-69
AC093568_14(AC093568|pid:none) Oryza sativa chromosome 10 clone ...   131   1e-29
AL035679_18(AL035679|pid:none) Arabidopsis thaliana DNA chromoso...   130   2e-29
AK221976_1(AK221976|pid:none) Arabidopsis thaliana mRNA for hypo...   130   2e-29
AK227321_1(AK227321|pid:none) Arabidopsis thaliana mRNA for hypo...   130   2e-29
AK230210_1(AK230210|pid:none) Arabidopsis thaliana mRNA for hypo...   130   3e-29
AK101151_1(AK101151|pid:none) Oryza sativa Japonica Group cDNA c...   126   4e-28
CP000588_396(CP000588|pid:none) Ostreococcus lucimarinus CCE9901...   122   5e-27
AK229960_1(AK229960|pid:none) Arabidopsis thaliana mRNA for vacu...   118   1e-25
protein update 2009. 7.12
PSORT 

 psg: 0.85  gvh: 0.62  alm: 0.18  top: 0.50  tms: 0.07  mit: 0.35  mip: 0.09
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.19  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: endoplasmic reticulum
	 20.0 %: mitochondrial
	 20.0 %: cytoplasmic
	 16.0 %: Golgi
	 12.0 %: nuclear
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for VHH270 is end
5' end seq. ID VHH270F
5' end seq. 
>VHH270F.Seq
AACTATTTAAAAAAAAATAGAGAATATAGTTATATATATAACACACAAAAAAATTCATAT
CACATTATTGTTTTTCATTAATTTATAAACAATAATATATATATAATAAACACTCTATCA
TTTAATTTTAATAAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 152
3' end seq. ID VHH270Z
3' end seq. 
>VHH270Z.Seq
NNNNNNNNNNGTCAAGGTGCTGTTCAAACCGCTCTCTTATTCTTGGCTCTCATTTCCATT
CCAGTTATGTTGGTCATTAAACCATTGTTCATGAAGAGATTCCACTTCCAAGAAGTCGAA
AGAAAGAAATTAGGTCACCACGAAGAGGAACATGATGATGAAGCTCTCTACACTGGTCAT
CACGGTGAAGAATTTGAAATGGGTGAAGTCTTTGTTCATCAAGTCATTCATACCATCGAG
TTTGTTTTGGGTGCTGTTTCAAATACTGCCTCTTATCTTCGTTTATGGGCTCTTTCATTG
GCTCACTCTGAGTTATCTTCTGTATTTTGGGAACGTATTCTCATTGGTCAAGTCGAACGT
GGTAATCCATTCTTGGCTTTCGTTGGTTTTGGTGCTTGNNTANGCTCCTCAGTGGATGT
Length of 3' end seq. 419
Connected seq. ID VHH270P
Connected seq. 
>VHH270P.Seq
AACTATTTAAAAAAAAATAGAGAATATAGTTATATATATAACACACAAAAAAATTCATAT
CACATTATTGTTTTTCATTAATTTATAAACAATAATATATATATAATAAACACTCTATCA
TTTAATTTTAATAAAAAAAAAA----------GTCAAGGTGCTGTTCAAACCGCTCTCTT
ATTCTTGGCTCTCATTTCCATTCCAGTTATGTTGGTCATTAAACCATTGTTCATGAAGAG
ATTCCACTTCCAAGAAGTCGAAAGAAAGAAATTAGGTCACCACGAAGAGGAACATGATGA
TGAAGCTCTCTACACTGGTCATCACGGTGAAGAATTTGAAATGGGTGAAGTCTTTGTTCA
TCAAGTCATTCATACCATCGAGTTTGTTTTGGGTGCTGTTTCAAATACTGCCTCTTATCT
TCGTTTATGGGCTCTTTCATTGGCTCACTCTGAGTTATCTTCTGTATTTTGGGAACGTAT
TCTCATTGGTCAAGTCGAACGTGGTAATCCATTCTTGGCTTTCGTTGGTTTTGGTGCTTG
NNTANGCTCCTCAGTGGATGT
Length of connected seq. 551
Full length Seq ID -
Full length Seq. -
Length of full length seq. -