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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHH251
Library VH
(Link to library)
Clone ID VHH251
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U14596-1
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>VHH251 (VHH251Q) /CSM/VH/VHH2-C/VHH251Q.Seq.d/
XXXXXXXXXXTCACCAGCACCATTTGCAACATTTGTTAAGNATTTAACATCAACTTCAAA
AGTTGTTTCACCACCATCTTACACTCAAAGATCCTTATCACATCATGGTTATATTCCAAA
ATCAACCACTAAATTAGTACCAAGAAGAGGCCCAAACAATGTAGATCTAGGTTTTTCAGT
TATACAAAATCAAAATGGTTTATTCCCAATTGATAAATTTATTACTAAACATTCAAAATC
TTTAAATATTAATACTACAAATGAAACTGAAGATACTTTAAGATCATTAAATACAAAGAG
TTCTTCATTATTTAATAATAATAATAATAATAATAATAATAATAACAATAGAAATGTTAA
TACAAATGTTAACGATTATCAAAATAATGGATTACCATCTTCTTCACTTTATAATAGTAA
TATTAATCAATTAAGTAACAACAACAATAATAATAATAATATTTATAATAT
sequence update 2002. 9.10
Translated Amino Acid sequence 
---PAPFATFVKXLTSTSKVVSPPSYTQRSLSHHGYIPKSTTKLVPRRGPNNVDLGFSVI
QNQNGLFPIDKFITKHSKSLNINTTNETEDTLRSLNTKSSSLFNNNNNNNNNNNNRNVNT
NVNDYQNNGLPSSSLYNSNINQLSNNNNNNNNIYN
Translated Amino Acid sequence (All Frames) 
Frame A:
---hqhhlqhllxi*hqlqklfhhhltlkdpyhimvifqnqpln*yqeeaqtm*i*vfql
ykikmvysqlinlllniqnl*ililqmklkil*dh*iqrvlhyliiiiiiiiiitiemli
qmltiikimdyhllhfiivilin*vtttiiiiifii


Frame B:
---tsticnic*xfninfkscfttilhskilitswlyskinh*istkkrpkqcrsrffsy
tkskwfipn**iyy*tfkifky*yyk*n*ryfkiikykeffii***********q*kc*y
kc*rlsk*witifftl***y*sik*qqq****yl*y


Frame C:
---PAPFATFVKXLTSTSKVVSPPSYTQRSLSHHGYIPKSTTKLVPRRGPNNVDLGFSVI
QNQNGLFPIDKFITKHSKSLNINTTNETEDTLRSLNTKSSSLFNNNNNNNNNNNNRNVNT
NVNDYQNNGLPSSSLYNSNINQLSNNNNNNNNIYN
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHO113 (VHO113Q) /CSM/VH/VHO1-A/VHO113Q.Seq.d/                        593   e-169
VHH251 (VHH251Q) /CSM/VH/VHH2-C/VHH251Q.Seq.d/                        593   e-169
SHG255 (SHG255Q) /CSM/SH/SHG2-C/SHG255Q.Seq.d/                        593   e-169
VHC265 (VHC265Q) /CSM/VH/VHC2-C/VHC265Q.Seq.d/                         44   0.002
CHM887 (CHM887Q) /CSM/CH/CHM8-D/CHM887Q.Seq.d/                         44   0.002
CHL551 (CHL551Q) /CSM/CH/CHL5-C/CHL551Q.Seq.d/                         44   0.002
AHN171 (AHN171Q) /CSM/AH/AHN1-C/AHN171Q.Seq.d/                         44   0.002
AHE402 (AHE402Q) /CSM/AH/AHE4-A/AHE402Q.Seq.d/                         44   0.002
SHF673 (SHF673Q) /CSM/SH/SHF6-D/SHF673Q.Seq.d/                         38   0.12
SLG418 (SLG418Q) /CSM/SL/SLG4-A/SLG418Q.Seq.d/                         36   0.46
own update 2004. 8. 3
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ441056) Dictyostelium discoideum cDNA clone:ddv45e14, 3' ...   593   e-169  2
(BJ445314) Dictyostelium discoideum cDNA clone:ddv59i03, 3' ...   593   e-169  2
(BJ406856) Dictyostelium discoideum cDNA clone:dds36m14, 3' ...   593   e-169  2
(BJ406576) Dictyostelium discoideum cDNA clone:dds35a20, 3' ...    38   0.058  2
(DU014473) 290198 Tomato MboI BAC Library Solanum lycopersic...    36   0.21   2
(AC200019) Spermophilus tridecemlineatus clone VMRC20-508N3,...    48   0.28   1
(AM976734) Sparus aurata EST, clone cDN13P0002I16, 5'-end se...    32   0.79   2
(AM973373) Sparus aurata EST, clone cDN11P0005D22, 5'-end se...    32   0.79   2
(AM954142) Sparus aurata EST, clone cDN02P0004P17, 5'-end se...    32   0.80   2
(EW981227) EST_sras_evg_782073 srasevg mixed_tissue Sebastes...    32   0.80   2
dna update 2008.11. 7
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54EQ8) RecName: Full=Nuclear pore complex protein Nup98-Nup96;...   201   5e-51
(Q4JK59) RecName: Full=Protein TET2; AltName: Full=Protein Ayu17...    35   0.83
DQ079067_1(DQ079067|pid:none) Mus musculus Ayu17-449 mRNA, compl...    35   0.83
CP001089_95(CP001089|pid:none) Geobacter lovleyi SZ, complete ge...    34   1.4
FM992688_937(FM992688|pid:none) Candida dubliniensis CD36 chromo...    32   5.4
AF167979_1(AF167979|pid:none) Dictyostelium discoideum protein p...    32   5.4
AB008268_2(AB008268|pid:none) Arabidopsis thaliana genomic DNA, ...    32   7.0
AM467639_2(AM467639|pid:none) Vitis vinifera contig VV78X003104....    32   9.1
protein update 2009. 3.10
PSORT 

 psg: 0.76  gvh: 0.47  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.48  mip: 0.08
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 68.0 %: mitochondrial
	 16.0 %: nuclear
	 12.0 %: cytoplasmic
	  4.0 %: cytoskeletal

>> prediction for VHH251 is mit
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID VHH251Z
3' end seq. 
>VHH251Z.Seq
NNNNNNNNNNTCACCAGCACCATTTGCAACATTTGTTAAGNATTTAACATCAACTTCAAA
AGTTGTTTCACCACCATCTTACACTCAAAGATCCTTATCACATCATGGTTATATTCCAAA
ATCAACCACTAAATTAGTACCAAGAAGAGGCCCAAACAATGTAGATCTAGGTTTTTCAGT
TATACAAAATCAAAATGGTTTATTCCCAATTGATAAATTTATTACTAAACATTCAAAATC
TTTAAATATTAATACTACAAATGAAACTGAAGATACTTTAAGATCATTAAATACAAAGAG
TTCTTCATTATTTAATAATAATAATAATAATAATAATAATAATAACAATAGAAATGTTAA
TACAAATGTTAACGATTATCAAAATAATGGATTACCATCTTCTTCACTTTATAATAGTAA
TATTAATCAATTAAGTAACAACAACAATAATAATAATAATATTTATAATAT
Length of 3' end seq. 471
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -