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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHH218
Library VH
(Link to library)
Clone ID VHH218
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHH218P
(Link to Original site)
Representative DNA sequence 
>VHH218 (VHH218Q) /CSM/VH/VHH2-A/VHH218Q.Seq.d/
ATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTGGTTGTACTTCACACGA
ATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCAGTTCANAAGTAACTGC
TGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCGTCGAAGGTGTTTGCGT
ACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCAAACCAGTCTCATTCGT
CAACAAAGTCGATCGTTTCTTATTANAACTCCAATTAAACACTGAAGAAGCTTATCTCTC
TTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACACTGAAGATAAGGAATT
CGGTGATGTCACTGTATCCCCAXXXXXXXXXXCTGATTTANGTTNCCNCNNTGCCGGTNA
AGNTTTNCCNCAANGNGTTTTNGATCANTGGGNTTCAANTGGNGNTGNCAACAAANATAA
AAAAGCCCCTGAANTNGCTTTANCCCCCAAAAAAAAAAAANGTTTAGCCCCAAAAATCCC
ANATTTTGACAAATTCCNCGAAAAACTNTGAACNANTTAAGTCGCCCNTTGAGNTTTCNA
AAATAGNAATCNAATTTAAAAAAAATTTTAAAAAAAAAATAATAAAAAAAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
SLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFSSXVTAALRVTDGALVVIDCVEGVCV
QTETVLRQAVAERIKPVSFVNKVDRFLLXLQLNTEEAYLSFRRAIESVNVIVGNTEDKEF
GDVTVSP---

---DLXXXXAGXXXPQXVXDXWXSXGXXNKXKKAPEXALXPKKKKXLAPKIPXFDKFXEK
L*tx*vax*xfxnxnxi*kkf*kknnkkkk
Translated Amino Acid sequence (All Frames) 
Frame A:
itslrnakgrqitswlyftrifnqsyrfprsr*fqfxsnccppcy*wcfsryrlrrrclr
tnrncitssrr*tyqtslirqqsrsflixtpikh*rslslfp*sy*irqchcw*h*r*gi
r*chcip---

---lixvxxxpvkxxxxxfxixgxqxxxxtxikkplxxlxppkkkxv*pqksxiltnsxk
nxexxkspxexskixixfkknfkkkiikkk

Frame B:
SLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFSSXVTAALRVTDGALVVIDCVEGVCV
QTETVLRQAVAERIKPVSFVNKVDRFLLXLQLNTEEAYLSFRRAIESVNVIVGNTEDKEF
GDVTVSP---

---*fxxpxcrxxfxxxxfxsxgfxwxxqqx*ksp*xxfxpqkkkxfspknpxf*qiprk
txnxlsrplxfxk*xsnlkkilkkk**kkk

Frame C:
hftskcqrktnyqlvvlhtnf*silsipqvtlisvxk*llpsvllmvl*slsiaskvfay
kpklyyvkpslnvsnqshsstksivsyxnsn*tlkklislsvellnpsmsllvtlkirns
vmslyp---

---DLXXXXAGXXXPQXVXDXWXSXGXXNKXKKAPEXALXPKKKKXLAPKIPXFDKFXEK
L*tx*vax*xfxnxnxi*kkf*kknnkkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHH218 (VHH218Q) /CSM/VH/VHH2-A/VHH218Q.Seq.d/                        805   0.0
VHQ469 (VHQ469Q) /CSM/VH/VHQ4-C/VHQ469Q.Seq.d/                        737   0.0
VHQ441 (VHQ441Q) /CSM/VH/VHQ4-B/VHQ441Q.Seq.d/                        737   0.0
VHQ438 (VHQ438Q) /CSM/VH/VHQ4-B/VHQ438Q.Seq.d/                        737   0.0
VHQ409 (VHQ409Q) /CSM/VH/VHQ4-A/VHQ409Q.Seq.d/                        737   0.0
VHQ395 (VHQ395Q) /CSM/VH/VHQ3-D/VHQ395Q.Seq.d/                        737   0.0
VHQ369 (VHQ369Q) /CSM/VH/VHQ3-C/VHQ369Q.Seq.d/                        737   0.0
VHQ359 (VHQ359Q) /CSM/VH/VHQ3-C/VHQ359Q.Seq.d/                        737   0.0
VHQ340 (VHQ340Q) /CSM/VH/VHQ3-B/VHQ340Q.Seq.d/                        737   0.0
VHQ311 (VHQ311Q) /CSM/VH/VHQ3-A/VHQ311Q.Seq.d/                        737   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(M26017) D.discoideum elongation factor 2 mRNA, complete cds.     737   0.0    2
(BJ422150) Dictyostelium discoideum cDNA clone:ddv45c06, 5' ...   745   0.0    1
(BJ427892) Dictyostelium discoideum cDNA clone:ddv63o22, 5' ...   737   0.0    1
(BJ427888) Dictyostelium discoideum cDNA clone:ddv63n23, 5' ...   737   0.0    1
(BJ427874) Dictyostelium discoideum cDNA clone:ddv63k23, 5' ...   737   0.0    1
(BJ427872) Dictyostelium discoideum cDNA clone:ddv63k21, 5' ...   737   0.0    1
(BJ427795) Dictyostelium discoideum cDNA clone:ddv63j18, 5' ...   737   0.0    1
(BJ427794) Dictyostelium discoideum cDNA clone:ddv63j17, 5' ...   737   0.0    1
(BJ427774) Dictyostelium discoideum cDNA clone:ddv63f15, 5' ...   737   0.0    1
(BJ427746) Dictyostelium discoideum cDNA clone:ddv63p09, 5' ...   737   0.0    1
dna update 2008.11. 7
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

D21259_1(D21259|pid:none) Entamoeba histolytica gene for elongat...   166   6e-40
(Q06193) RecName: Full=Elongation factor 2;          Short=EF-2;...   166   6e-40
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   166   7e-40
AM494972_26(AM494972|pid:none) Leishmania braziliensis chromosom...   166   7e-40
CT005272_19(CT005272|pid:none) Leishmania major strain Friedlin,...   165   1e-39
EU624179_1(EU624179|pid:none) Lomentaria orcadensis elongation f...   164   2e-39
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   164   3e-39
EU624167_1(EU624167|pid:none) Gastroclonium clavatum elongation ...   164   3e-39
EF033570_1(EF033570|pid:none) Plocamium maggsiae voucher G0167 e...   163   4e-39
EF033551_1(EF033551|pid:none) Dilsea carnosa voucher GWS000746 e...   163   5e-39
protein update 2009. 7.12
PSORT 

 psg: 0.75  gvh: 0.46  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.13  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: cytoplasmic
	 32.0 %: nuclear
	  4.0 %: cytoskeletal

>> prediction for VHH218 is cyt
5' end seq. ID VHH218F
5' end seq. 
>VHH218F.Seq
ATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTGGTTGTACTTCACACGA
ATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCAGTTCANAAGTAACTGC
TGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCGTCGAAGGTGTTTGCGT
ACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCAAACCAGTCTCATTCGT
CAACAAAGTCGATCGTTTCTTATTANAACTCCAATTAAACACTGAAGAAGCTTATCTCTC
TTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACACTGAAGATAAGGAATT
CGGTGATGTCACTGTATCCCCANNNNNNNNNN
Length of 5' end seq. 392
3' end seq. ID VHH218Z
3' end seq. 
>VHH218Z.Seq
NNNNNNNNNNCTGATTTANGTTNCCNCNNTGCCGGTNAAGNTTTNCCNCAANGNGTTTTN
GATCANTGGGNTTCAANTGGNGNTGNCAACAAANATAAAAAAGCCCCTGAANTNGCTTTA
NCCCCCAAAAAAAAAAAANGTTTAGCCCCAAAAATCCCANATTTTGACAAATTCCNCGAA
AAACTNTGAACNANTTAAGTCGCCCNTTGAGNTTTCNAAAATAGNAATCNAATTTAAAAA
AAATTTTAAAAAAAAAATAATAAAAAAAAAAAA
Length of 3' end seq. 273
Connected seq. ID VHH218P
Connected seq. 
>VHH218P.Seq
ATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTGGTTGTACTTCACACGA
ATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCAGTTCANAAGTAACTGC
TGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCGTCGAAGGTGTTTGCGT
ACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCAAACCAGTCTCATTCGT
CAACAAAGTCGATCGTTTCTTATTANAACTCCAATTAAACACTGAAGAAGCTTATCTCTC
TTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACACTGAAGATAAGGAATT
CGGTGATGTCACTGTATCCCCA----------CTGATTTANGTTNCCNCNNTGCCGGTNA
AGNTTTNCCNCAANGNGTTTTNGATCANTGGGNTTCAANTGGNGNTGNCAACAAANATAA
AAAAGCCCCTGAANTNGCTTTANCCCCCAAAAAAAAAAAANGTTTAGCCCCAAAAATCCC
ANATTTTGACAAATTCCNCGAAAAACTNTGAACNANTTAAGTCGCCCNTTGAGNTTTCNA
AAATAGNAATCNAATTTAAAAAAAATTTTAAAAAAAAAATAATAAAAAAAAAAAA
Length of connected seq. 645
Full length Seq ID -
Full length Seq. -
Length of full length seq. -