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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHH212
Library VH
(Link to library)
Clone ID VHH212
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHH212P
(Link to Original site)
Representative DNA sequence 
>VHH212 (VHH212Q) /CSM/VH/VHH2-A/VHH212Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCAGGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTGTATCCCCAGXXXXXXXXXXTTGATCATTGG
GNTTCAANTGGNGNTGTCAANAAANATAAAAAAGCCNCTGAAGTNGCTTTANCCCCCAAA
AAAAAAAAANGTTTAGCCCCANAAATNCCANATTTTGACAAATTCCCCGAAAAACNNTAA
ACNANTTAAGTCGCC
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSGRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTEDKEFGDVTVSP---

---DHWXSXGXVXKXKKAXEVALXPKKKKXLAPXXPXFDKFPEKX*tx*va
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dicqvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlkirnsvmslypq---

---liigxqxxxsxxikkplkxlxppkkkxv*pxkxxiltnspknxkxxks

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvrsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*h*r*gir*chcip---

---*slgfxwxcqxx*ksx*sxfxpqkkkxfspxnxxf*qiprktxnxlsr

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSGRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTEDKEFGDVTVSP---

---DHWXSXGXVXKXKKAXEVALXPKKKKXLAPXXPXFDKFPEKX*tx*va
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHH212 (VHH212Q) /CSM/VH/VHH2-A/VHH212Q.Seq.d/                       1269   0.0
VHQ469 (VHQ469Q) /CSM/VH/VHQ4-C/VHQ469Q.Seq.d/                       1219   0.0
VHQ441 (VHQ441Q) /CSM/VH/VHQ4-B/VHQ441Q.Seq.d/                       1219   0.0
VHQ438 (VHQ438Q) /CSM/VH/VHQ4-B/VHQ438Q.Seq.d/                       1219   0.0
VHQ395 (VHQ395Q) /CSM/VH/VHQ3-D/VHQ395Q.Seq.d/                       1219   0.0
VHQ369 (VHQ369Q) /CSM/VH/VHQ3-C/VHQ369Q.Seq.d/                       1219   0.0
VHQ311 (VHQ311Q) /CSM/VH/VHQ3-A/VHQ311Q.Seq.d/                       1219   0.0
VHQ288 (VHQ288Q) /CSM/VH/VHQ2-D/VHQ288Q.Seq.d/                       1219   0.0
VHQ248 (VHQ248Q) /CSM/VH/VHQ2-B/VHQ248Q.Seq.d/                       1219   0.0
VHQ212 (VHQ212Q) /CSM/VH/VHQ2-A/VHQ212Q.Seq.d/                       1219   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ422166) Dictyostelium discoideum cDNA clone:ddv45g04, 5' ...  1225   0.0    1
(BJ427892) Dictyostelium discoideum cDNA clone:ddv63o22, 5' ...  1217   0.0    1
(BJ427888) Dictyostelium discoideum cDNA clone:ddv63n23, 5' ...  1217   0.0    1
(BJ427874) Dictyostelium discoideum cDNA clone:ddv63k23, 5' ...  1217   0.0    1
(BJ427872) Dictyostelium discoideum cDNA clone:ddv63k21, 5' ...  1217   0.0    1
(BJ427795) Dictyostelium discoideum cDNA clone:ddv63j18, 5' ...  1217   0.0    1
(BJ427794) Dictyostelium discoideum cDNA clone:ddv63j17, 5' ...  1217   0.0    1
(BJ427744) Dictyostelium discoideum cDNA clone:ddv63o12, 5' ...  1217   0.0    1
(BJ427732) Dictyostelium discoideum cDNA clone:ddv63m09, 5' ...  1217   0.0    1
(BJ427728) Dictyostelium discoideum cDNA clone:ddv63l10, 5' ...  1217   0.0    1
dna update 2008.11. 7
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   272   8e-72
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   267   3e-70
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   266   4e-70
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   266   7e-70
AM494972_26(AM494972|pid:none) Leishmania braziliensis chromosom...   266   7e-70
CT005272_19(CT005272|pid:none) Leishmania major strain Friedlin,...   265   1e-69
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   265   1e-69
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   265   2e-69
AB095183_1(AB095183|pid:none) Cyanidioschyzon merolae gene for e...   263   6e-69
CU638744_366(CU638744|pid:none) Podospora anserina genomic DNA c...   263   6e-69
protein update 2009. 7.12
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.13  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 56.0 %: cytoplasmic
	 28.0 %: nuclear
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar
	  4.0 %: plasma membrane

>> prediction for VHH212 is cyt
5' end seq. ID VHH212F
5' end seq. 
>VHH212F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCAGGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTGTATCCCCAGNNNNNNNNNN
Length of 5' end seq. 649
3' end seq. ID VHH212Z
3' end seq. 
>VHH212Z.Seq
NNNNNNNNNNTTGATCATTGGGNTTCAANTGGNGNTGTCAANAAANATAAAAAAGCCNCT
GAAGTNGCTTTANCCCCCAAAAAAAAAAAANGTTTAGCCCCANAAATNCCANATTTTGAC
AAATTCCCCGAAAAACNNTAAACNANTTAAGTCGCC
Length of 3' end seq. 156
Connected seq. ID VHH212P
Connected seq. 
>VHH212P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCAGGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATAAGGAATTCGGTGATGTCACTGTATCCCCAG----------TTGATCATTGG
GNTTCAANTGGNGNTGTCAANAAANATAAAAAAGCCNCTGAAGTNGCTTTANCCCCCAAA
AAAAAAAAANGTTTAGCCCCANAAATNCCANATTTTGACAAATTCCCCGAAAAACNNTAA
ACNANTTAAGTCGCC
Length of connected seq. 785
Full length Seq ID -
Full length Seq. -
Length of full length seq. -