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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHH149
Library VH
(Link to library)
Clone ID VHH149
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID
(Link to Original site)
Representative DNA sequence 
>VHH149 (VHH149Q) /CSM/VH/VHH1-C/VHH149Q.Seq.d/
XXXXXXXXXXTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCTG
AAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTTT
CAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAAC
AACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAA
ATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATTG
AATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATG
AATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCT
ATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCAC
CAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAACA
TCTCTATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAATCAATAAAGCTTCTCAT
ATA
sequence update 2002. 9.10
Translated Amino Acid sequence 
---EVTFDVDANGILNVSAEDKSTGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQ
QKDRVESKNKLENYAFTVKNSIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDE
YEDKMKALEAVVNPIMSKLYQEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseeni
sik*is*kiis**nq*sfsy
Translated Amino Acid sequence (All Frames) 
Frame A:
---EVTFDVDANGILNVSAEDKSTGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQ
QKDRVESKNKLENYAFTVKNSIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDE
YEDKMKALEAVVNPIMSKLYQEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseeni
sik*is*kiis**nq*sfsy


Frame B:
---klhlmlmqmvs*myqlkinqlvtnkrlqlpmikvvfqkkklkkwllmqrnsnnkmnn
kkivlnqrinwkimhsllkiqlkmkklqpkfqiqinqllnlklkvysng*nqikpqkrmn
mkir*kp*kqlsiqs*vnsikkevchkvvvcqvvcqmihqrvqttk*tn*idgifqrkts
lsnrflkk**vskinkashi


Frame C:
---syi*c*ckwylkcis*r*inw*qtkdynyq**rssfkrrn*kngc*creiqttr*tt
krsc*ike*igklcihc*kfn*r*kscsqnfrfr*iny*i*n*kctqmvriksnrrkg*i
*r*deslrsscqsnhe*tlsrrryatrwwyarwyvk*ftkefkqqsrrirlteffrgkhl
yqidflknnklvksiklli
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSH453 (VSH453Q) /CSM/VS/VSH4-C/VSH453Q.Seq.d/                       1176   0.0
VHQ260 (VHQ260Q) /CSM/VH/VHQ2-C/VHQ260Q.Seq.d/                       1176   0.0
VHP719 (VHP719Q) /CSM/VH/VHP7-A/VHP719Q.Seq.d/                       1176   0.0
VHP716 (VHP716Q) /CSM/VH/VHP7-A/VHP716Q.Seq.d/                       1176   0.0
VHP437 (VHP437Q) /CSM/VH/VHP4-B/VHP437Q.Seq.d/                       1176   0.0
VHP420 (VHP420Q) /CSM/VH/VHP4-A/VHP420Q.Seq.d/                       1176   0.0
VHP266 (VHP266Q) /CSM/VH/VHP2-C/VHP266Q.Seq.d/                       1176   0.0
VHP236 (VHP236Q) /CSM/VH/VHP2-B/VHP236Q.Seq.d/                       1176   0.0
VHP232 (VHP232Q) /CSM/VH/VHP2-B/VHP232Q.Seq.d/                       1176   0.0
VHP224 (VHP224Q) /CSM/VH/VHP2-A/VHP224Q.Seq.d/                       1176   0.0
own update 2002.12.29
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(X75263) D.discoideum (AX3) mRNA for heat shock cognate prot...  1176   0.0    1
(AU267475) Dictyostelium discoideum vegetative cDNA clone:VS...  1176   0.0    1
(AU039542) Dictyostelium discoideum slug cDNA, clone SLE721.     1176   0.0    1
(AU039449) Dictyostelium discoideum slug cDNA, clone SLH812.     1176   0.0    1
(AU034156) Dictyostelium discoideum slug cDNA, clone SLC111.     1176   0.0    1
(C92565) Dictyostelium discoideum slug cDNA, clone SSE694.       1176   0.0    1
(C90043) Dictyostelium discoideum slug cDNA, clone SSG133.       1176   0.0    1
(BJ446704) Dictyostelium discoideum cDNA clone:ddv63g16, 3' ...  1176   0.0    1
(BJ446275) Dictyostelium discoideum cDNA clone:ddv62p03, 3' ...  1176   0.0    1
(BJ446228) Dictyostelium discoideum cDNA clone:ddv62f05, 3' ...  1176   0.0    1
dna update 2008.11. 6
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   267   1e-70
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   267   1e-70
AF033852_1(AF033852|pid:none) Spinacia oleracea cytosolic heat s...   178   9e-44
(Q54BE0) RecName: Full=Heat shock cognate 70 kDa protein 3;           177   3e-43
FB790108_1(FB790108|pid:none) Sequence 9381 from Patent WO200803...   176   3e-43
(P24629) RecName: Full=Heat shock cognate 70 kDa protein 1;  &FB...   176   4e-43
AY253326_1(AY253326|pid:none) Nicotiana tabacum heat shock prote...   174   1e-42
(Q557E0) RecName: Full=Heat shock cognate 70 kDa protein 2;     ...   174   2e-42
AF025951_1(AF025951|pid:none) Dictyostelium discoideum heat-shoc...   174   2e-42
FB790112_1(FB790112|pid:none) Sequence 9385 from Patent WO200803...   174   2e-42
protein update 2009. 7.12
PSORT 

 psg: 0.75  gvh: 0.33  alm: 0.55  top: 0.53  tms: 0.00  mit: 0.12  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: cytoskeletal
	 12.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VHH149 is nuc
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID VHH149Z
3' end seq. 
>VHH149Z.Seq
NNNNNNNNNNTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTATCAGCTG
AAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGTCGTCTTT
CAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAAC
AACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAA
ATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATTG
AATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATG
AATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCT
ATCAAGAAGGAGGTATGCCACAAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCAC
CAAAGAGTTCAAACAACAAAGTAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAACA
TCTCTATCAAATAGATTTCTTAAAAAATAATAAGTTAGTAAAATCAATAAAGCTTCTCAT
ATA
Length of 3' end seq. 603
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -